Antimicrobial peptide HPA3NT3-A2 effectively inhibits biofilm formation in mice infected with drug-resistant bacteria

2019 ◽  
Vol 7 (12) ◽  
pp. 5068-5083 ◽  
Author(s):  
Jong-Kook Lee ◽  
Loredana Mereuta ◽  
Tudor Luchian ◽  
Yoonkyung Park
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Peptide ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Resistant Bacteria ◽  
Bacterial Biofilms ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Serious Infections

Bacterial biofilms formed through secretion of extracellular polymeric substances (EPS) have been implicated in many serious infections and can increase antibiotic resistance by a factor of more than 1000.

scholarly journals Novel Seleno- and Thio-Urea Containing Dihydropyrrol-2-One Analogues as Antibacterial Agents

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 321
Author(s):  
Shekh Sabir ◽  
Tsz Tin Yu ◽  
Rajesh Kuppusamy ◽  
Basmah Almohaywi ◽  
George Iskander ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Quorum Sensing ◽  
Antibacterial Agents ◽  
Inhibitory Concentration ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Positive Bacterium ◽  
Quorum Sensing Inhibition ◽  
Drug Resistant Bacteria

The quorum sensing (QS) system in multi-drug-resistant bacteria such as P. aeruginosa is primarily responsible for the development of antibiotic resistance and is considered an attractive target for antimicrobial drug discovery. In this study, we synthesised a series of novel selenourea and thiourea-containing dihydropyrrol-2-one (DHP) analogues as LasR antagonists. The selenium DHP derivatives displayed significantly better quorum-sensing inhibition (QSI) activities than the corresponding sulphur analogues. The most potent analogue 3e efficiently inhibited the las QS system by 81% at 125 µM and 53% at 31 µM. Additionally, all the compounds were screened for their minimum inhibitory concentration (MIC) against the Gram-positive bacterium S. aureus, and interestingly, only the selenium analogues showed antibacterial activity, with 3c and 3e being the most potent with a MIC of 15.6 µM.

scholarly journals All d-Lysine Analogues of the Antimicrobial Peptide HPA3NT3-A2 Increased Serum Stability and without Drug Resistance

2020 ◽  
Vol 21 (16) ◽  
pp. 5632
Author(s):  
Jong-Kook Lee ◽  
Yoonkyung Park
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Peptide ◽  
Proteolytic Enzymes ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Peptide Bonds ◽  
Drug Resistant Bacteria ◽  
Antibiotic Drugs ◽  
Strong Antimicrobial Activity

Novel antibiotic drugs are urgently needed because of the increase in drug-resistant bacteria. The use of antimicrobial peptides has been suggested to replace antibiotics as they have strong antimicrobial activity and can be extracted from living organisms such as insects, marine organisms, and mammals. HPA3NT3-A2 ([Ala1,8] HPA3NT3) is an antimicrobial peptide that is an analogue of the HP (2–20) peptide derived from Helicobacter pylori ribosomal protein L1. Although this peptide was shown to have strong antimicrobial activity against drug-resistant bacteria, it also showed lower toxicity against sheep red blood cells (RBCs) and HaCaT cells compared to HPA3NT3. The l-Lys residues of HPA3NT3-A2 was substituted with d-Lys residues (HPA3NT3-A2D; [d-Lys2,5,6,9,10,15] HPA3NT3-A2) to prevent the cleavage of peptide bonds by proteolytic enzymes under physiological conditions. This peptide showed an increased half-life and maintained its antimicrobial activity in the serum against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) (pathogen). Furthermore, the antimicrobial activity of HPA3NT3-A2D was not significantly affected in the presence of mono- or divalent ions (Na+, Mg2+, Ca2+). Finally, l- or d-HPA3NT3-A2 peptides exhibited the strongest antimicrobial activity against antibiotic-resistant bacteria and failed to induce resistance in Staphylococcus aureus after 12 passages.

Silver nanoflower coupled with low dose antibiotics enables highly effective Drug-resistant bacteria eradication

2021 ◽  
Author(s):  
Xin Li ◽  
Xianting Ding ◽  
Khan Zara Ahmad ◽  
Jie He ◽  
Hongxia Li ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Low Dose ◽  
Multidrug Resistant ◽  
Effective Drug ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Multidrug Resistant Bacteria ◽  
Effective Strategies ◽  
Drug Resistant Bacteria ◽  
Highly Effective

Due to the global overuse of antibiotics, multidrug-resistant bacteria (MDR) continuously call for effective strategies to tackle the antibiotic resistance crisis. Here, we develop a silver nanomaterial with a petal-like...

Biotechnological War against Biofilms. Could Phages Mean the End of Device-Related Infections?

2007 ◽  
Vol 30 (9) ◽  
pp. 805-812 ◽  
Author(s):  
J.L. Del Pozo ◽  
M. Alonso ◽  
C.R. Arciola ◽  
R. Gonzalez ◽  
J. Leiva ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Biofilm Formation ◽  
Therapeutic Agents ◽  
Antibiotic Activity ◽  
Therapeutic Use ◽  
Resistant Bacteria ◽  
Clinical Settings ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Great Prevalence

Microorganisms universally attach to surfaces, resulting in biofilm formation. These biofilms entail a serious problem in daily clinical practice because of the great prevalence of implantable device-related infections. Differences in antibiotic activity against planktonic and sessile bacteria may relate to clinical failures in the treatment of biofilm-related infections (BRI). Bacteriophages have several characteristics that make them potentially attractive therapeutic agents in some selected clinical settings, like for example BRI. They are highly specific and very effective in lysing targeted bacteria, moreover, they appear to be safe for humans. Many studies have shown the potential of phages for the treatment of infectious diseases in plants and animals, including infections with highly drug-resistant bacteria. The therapeutic use of bacteriophages, possibly in combination with antibiotics, may be a valuable approach in BRI. However, many important questions still remain that must be addressed before phages can be endorsed for therapeutic use in humans.

scholarly journals NATURAL ANTIMICROBIALS IN THE PIPELINE AND POSSIBLE SYNERGISM WITH ANTIBIOTICS TO OVERCOME MICROBIAL RESISTANCE

2019 ◽  
pp. 15-21
Author(s):  
EMTENAN M HANAFI ◽  
ENAS N DANIAL
Keyword(s):  
Quorum Sensing ◽  
Bioactive Compounds ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Quorum Sensing Inhibitors ◽  
Drug Resistant Bacteria ◽  
Multiple Drug Resistant

The unresponsive use of antibiotics led to the appearance of multiple drug-resistant bacteria strains. Studying the mechanism by which bacteria can resist antibiotics, the so called quorum sensing and biofilm formation, enabled the researchers to find bioactive compounds, derived from eukaryotes and prokaryotes. The disrupt of this mechanism is called quorum sensing inhibitors or quorum quenchers. This article provides an overview on the current research done on such bioactive compounds, the possible use of them as antibiotic alternatives, what are the advantage and disadvantages, the source from which it has been extracted, and how it may succeed to overcome bacterial resistance. The recommendation of researchers is to use some of these natural antimicrobial compounds combined to lower doses of antibiotics for treatment, the fastest way to limit the adverse effects of the exploitation of antibiotics and to avoid bacterial resistance.

Photosensitized reactive chlorine species-mediated therapeutic destruction of drug-resistant bacteria using plasmonic core–shell Ag@AgCl nanocubes as an external nanomedicine

Nanoscale ◽  
2020 ◽  
Vol 12 (24) ◽  
pp. 12970-12984 ◽  
Author(s):  
Suresh Thangudu ◽  
Sagar Sunil Kulkarni ◽  
Raviraj Vankayala ◽  
Chi-Shiun Chiang ◽  
Kuo Chu Hwang
Keyword(s):  
Antibiotic Resistance ◽  
Rapid Growth ◽  
Bacterial Infections ◽  
Resistant Bacteria ◽  
Core Shell ◽  
Drug Resistant ◽  
Bacterial Antibiotic Resistance ◽  
Drug Resistant Bacteria

Due to the rapid growth of drug-resistant bacterial infections, there is an urgent need to develop innovative antimicrobial strategies to conquer the bacterial antibiotic resistance problems.

Integration of antimicrobial peptides and gold nanorods for bimodal antibacterial applications

2020 ◽  
Vol 8 (16) ◽  
pp. 4447-4457
Author(s):  
Jin Chen ◽  
Tingting Dai ◽  
Jiawei Yu ◽  
Xiahong Dai ◽  
Richai Chen ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Antimicrobial Peptide ◽  
Gold Nanorods ◽  
Bacterial Activity ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Resistant Bacteria

We integrate the anti-bacterial activity of BF2b antimicrobial peptide and the photothermal sterilization of gold nanorods to kill drug-resistant bacteria.

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