Evaluation of the anticarcinogenic effect of solanumrastratum dunal (Mexican thistle) on breast cancer cells MDA.

84 Background: The use of medicinal plants has been very common since ancient times, used for the treatment of several diseases such as cancer. In many towns in Mexico are used extracts from plants such as Solanum rastratum Dunal (buffalo bur, spiny nightshade, Colorado bur, Kansas thistle, Mexican thistle, and Texas thistle), which has shown to be effective in improving symptoms of some diseases conditions, there are reports indicating its use in the treatment of some types of cancer, such as the breast and cervical cancer. For this reason, in this work the aim was to evaluate the anticarcinogenic effect of extracts of Solanum rastratum Dunal on human breast cancer cells MDA. Methods: The plant was collected, cleaned, separate the different sections, were dried in darkness in special panels at room temperature; subsequently the extracts were prepared from the leaf, stem and flower-fruit using solvents of different polarity (methanol, ethyl acetate and hexane). Extracts were analyzed in cultured MDA cells line and the cytotoxic effect was evaluated by MTT technique. Results: The results showed that the stem and leaf extracts were those which showed higher inhibitory effect on cell viability, with those obtained with ethyl acetate which showed better anti-carcinogenic effect, while the hexane extract of the flower and fruit mixture which was showed higher inhibitory effect on this part of the plant; however generally the ethyl acetate extract obtained from the leaf was the one with greater cytotoxic effect in this cell line. Conclusions: The results of this work lead to the conclusion that is greater cytotoxic effect of ethyl acetate extract of the leaf than the other extracts tested. These results are contrasting with the traditional use of the extract of this plant, since traditionally people employ the aqueous extract of this plant, which would be equivalent to methanolic extract used in this study, which was who presented the lower cytotoxic effect of three solvents studied.

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Correction: Ou-Yang, F. et al. Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima). Int. J. Mol. Sci. 2019, 20, 3238

International Journal of Molecular Sciences ◽

10.3390/ijms22020668 ◽

2021 ◽

Vol 22 (2) ◽

pp. 668

Author(s):

Fu Ou-Yang ◽

I-Hsuan Tsai ◽

Jen-Yang Tang ◽

Ching-Yu Yen ◽

Yuan-Bin Cheng ◽

...

Keyword(s):

Breast Cancer ◽

Ethyl Acetate ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Ethyl Acetate Extract ◽

Published Paper

The authors would like to make corrections to their published paper [...]

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Crude ethyl acetate extract of marine microalga,Chaetoceros calcitrans, induces Apoptosis in MDA-MB-231 breast cancer cells

Pharmacognosy Magazine ◽

10.4103/0973-1296.126650 ◽

2014 ◽

Vol 10 (37) ◽

pp. 1 ◽

Cited By ~ 8

Author(s):

SuPeng Loh ◽

Noorjahan BanuMohamed Alitheen ◽

SuHua Goh ◽

FatimahMd Yusoff ◽

SweeKeong Yap

Keyword(s):

Breast Cancer ◽

Ethyl Acetate ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Ethyl Acetate Extract ◽

Marine Microalga ◽

Chaetoceros Calcitrans

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Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima)

International Journal of Molecular Sciences ◽

10.3390/ijms20133238 ◽

2019 ◽

Vol 20 (13) ◽

pp. 3238 ◽

Cited By ~ 3

Author(s):

Fu Ou-Yang ◽

I-Hsuan Tsai ◽

Jen-Yang Tang ◽

Ching-Yu Yen ◽

Yuan-Bin Cheng ◽

...

Keyword(s):

Breast Cancer ◽

Oxidative Stress ◽

Dna Damage ◽

Ethyl Acetate ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Ethyl Acetate Extract ◽

Mechanistic Study ◽

Breast Cancer Cell Lines ◽

Antitumor Effects

Extracts from the Nepenthes plant have anti-microorganism and anti-inflammation effects. However, the anticancer effect of the Nepenthes plant is rarely reported, especially for breast cancer cells. Here, we evaluate the antitumor effects of the ethyl acetate extract of Nepenthes thorellii x (ventricosa x maxima) (EANT) against breast cancer cells. Cell viability and flow cytometric analyses were used to analyze apoptosis, oxidative stress, and DNA damage. EANT exhibits a higher antiproliferation ability to two breast cancer cell lines (MCF7 and SKBR3) as compared to normal breast cells (M10). A mechanistic study demonstrates that EANT induces apoptosis in breast cancer cells with evidence of subG1 accumulation and annexin V increment. EANT also induces glutathione (GSH) depletion, resulting in dramatic accumulations of reactive oxygen species (ROS) and mitochondrial superoxide (MitoSOX), as well as the depletion of mitochondrial membrane potential (MMP). These oxidative stresses attack DNA, respectively leading to DNA double strand breaks and oxidative DNA damage in γH2AX and 8-oxo-2′deoxyguanosine (8-oxodG) assays. Overall these findings clearly revealed that EANT induced changes were suppressed by the ROS inhibitor. In conclusion, our results have shown that the ROS-modulating natural product (EANT) has antiproliferation activity against breast cancer cells through apoptosis, oxidative stress, and DNA damage.

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Monascus-fermented red mold rice exhibits cytotoxic effect and induces apoptosis on human breast cancer cells

Applied Microbiology and Biotechnology ◽

10.1007/s00253-012-4279-6 ◽

2012 ◽

Vol 97 (3) ◽

pp. 1269-1278 ◽

Cited By ~ 19

Author(s):

Chu-I Lee ◽

Chun-Lin Lee ◽

Jyi-Faa Hwang ◽

Yi-Hsin Lee ◽

Jyh-Jye Wang

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Cytotoxic Effect ◽

Human Breast Cancer Cells ◽

Human Breast ◽

Red Mold Rice

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Combined Luteolin and Indole-3-Carbinol Synergistically Constrains ERα-Positive Breast Cancer by Dual Inhibiting Estrogen Receptor Alpha and Cyclin-Dependent Kinase 4/6 Pathway in Cultured Cells and Xenograft Mice

Cancers ◽

10.3390/cancers13092116 ◽

2021 ◽

Vol 13 (9) ◽

pp. 2116

Author(s):

Xiaoyong Wang ◽

Lijuan Zhang ◽

Qi Dai ◽

Hongzong Si ◽

Longyun Zhang ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cell ◽

Breast Cancer Cells ◽

Cultured Cells ◽

Inhibitory Effect ◽

Cyclin Dependent Kinase ◽

Xenograft Mice ◽

The Individual

The high concentrations of individual phytochemicals in vitro studies cannot be physiologically achieved in humans. Our solution for this concentration gap between in vitro and human studies is to combine two or more phytochemicals. We screened 12 phytochemicals by pairwise combining two compounds at a low level to select combinations exerting the synergistic inhibitory effect of breast cancer cell proliferation. A novel combination of luteolin at 30 μM (LUT30) and indole-3-carbinol 40 μM (I3C40) identified that this combination (L30I40) synergistically constrains ERα+ breast cancer cell (MCF7 and T47D) proliferation only, but not triple-negative breast cancer cells. At the same time, the individual LUT30 and I3C40 do not have this anti-proliferative effect in ERα+ breast cancer cells. Moreover, this combination L30I40 does not have toxicity on endothelial cells compared to the current commercial drugs. Similarly, the combination of LUT and I3C (LUT10 mg + I3C10 mg/kg/day) (IP injection) synergistically suppresses tumor growth in MCF7 cells-derived xenograft mice, but the individual LUT (10 mg/kg/day) and I3C (20 mg/kg/day) do not show an inhibitory effect. This combination synergistically downregulates two major therapeutic targets ERα and cyclin dependent kinase (CDK) 4/6/retinoblastoma (Rb) pathway, both in cultured cells and xenograft tumors. These results provide a solid foundation that a combination of LUT and I3C may be a practical approach to treat ERα+ breast cancer cells after clinical trials.

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Inhibitory effect of BMP-2 on the proliferation of breast cancer cells

Molecular Medicine Reports ◽

10.3892/mmr.2012.962 ◽

2012 ◽

Vol 6 (3) ◽

pp. 615-620 ◽

Cited By ~ 16

Author(s):

ANAN CHEN ◽

DINGDING WANG ◽

XUETING LIU ◽

SHUILIAN HE ◽

ZHIHONG YU ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Inhibitory Effect

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A novel radiolytic rotenone derivative, rotenoisin A, displays potent anticarcinogenic activity in breast cancer cells

Journal of Radiation Research ◽

10.1093/jrr/rrab005 ◽

2021 ◽

Author(s):

Dong-ho Bak ◽

Seong Hee Kang ◽

Chul-hong Park ◽

Byung Yeoup Chung ◽

Hyoung-Woo Bai

Keyword(s):

Breast Cancer ◽

Adverse Effects ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Parent Compound ◽

Inhibitory Effect ◽

Chemotherapeutic Agents ◽

Epidermal Keratinocytes ◽

Functional Changes ◽

Structural Modifications

Abstract Chemotherapy for cancer treatment has therapeutic limitations, such as drug resistance, excessive toxic effects and undesirable adverse effects. Therefore, efforts to improve the safety and efficacy of chemotherapeutic agents are essential. Ionizing radiation can improve physiological and pharmacological properties by transforming structural modifications of the drug. In this study, in order to reduce the adverse effects of rotenone and increase anticancer activity, a new radiolytic rotenone derivative called rotenoisin A was generated through radiolytic transformation. Our findings showed that rotenoisin A inhibited the proliferation of breast cancer cells and increased the rate of apoptosis, whereas it had no inhibitory effect on primary epidermal keratinocytes compared with rotenone. Moreover, rotenoisin A-induced DNA damage by increasing reactive oxygen species (ROS) accumulation. It was also confirmed not only to alter the composition ratio of mitochondrial proteins, but also to result in structural and functional changes. The anticancer effect and molecular signalling mechanisms of rotenoisin A were consistent with those of rotenone, as previously reported. Our study suggests that radiolytic transformation of highly toxic compounds may be an alternative strategy for maintaining anticancer effects and reducing the toxicity of the parent compound.

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