scholarly journals Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa

2021 ◽  
Vol 22 (5) ◽  
pp. 2773
Author(s):  
Mika Yamanaka-Takaichi ◽  
Yukari Mizukami ◽  
Koji Sugawara ◽  
Kishiko Sunami ◽  
Yuichi Teranishi ◽  
...  
Keyword(s):  
Mast Cell ◽  
Nasal Mucosa ◽  
Perceived Stress ◽  
Ex Vivo ◽  
Neutralizing Antibody ◽  
Nasal Allergy ◽  
Corticotropin Releasing Hormone ◽  
Human Nasal Mucosa ◽  
Releasing Hormone

Psychological stress exacerbates mast cell (MC)-dependent inflammation, including nasal allergy, but the underlying mechanisms are not thoroughly understood. Because the key stress-mediating neurohormone, corticotropin-releasing hormone (CRH), induces human skin MC degranulation, we hypothesized that CRH may be a key player in stress-aggravated nasal allergy. In the current study, we probed this hypothesis in human nasal mucosa MCs (hM-MCs) in situ using nasal polyp organ culture and tested whether CRH is required for murine M-MC activation by perceived stress in vivo. CRH stimulation significantly increased the number of hM-MCs, stimulated both their degranulation and proliferation ex vivo, and increased stem cell factor (SCF) expression in human nasal mucosa epithelium. CRH also sensitized hM-MCs to further CRH stimulation and promoted a pro-inflammatory hM-MC phenotype. The CRH-induced increase in hM-MCs was mitigated by co-administration of CRH receptor type 1 (CRH-R1)-specific antagonist antalarmin, CRH-R1 small interfering RNA (siRNA), or SCF-neutralizing antibody. In vivo, restraint stress significantly increased the number and degranulation of murine M-MCs compared with sham-stressed mice. This effect was mitigated by intranasal antalarmin. Our data suggest that CRH is a major activator of hM-MC in nasal mucosa, in part via promoting SCF production, and that CRH-R1 antagonists such as antalarmin are promising candidate therapeutics for nasal mucosa neuroinflammation induced by perceived stress.

In vivo action of a new octadecaneuropeptide antagonist on neuropeptide Y and corticotropin-releasing hormone mRNA levels in rat

2005 ◽  
Vol 141 (2) ◽  
pp. 156-160 ◽  
Author(s):  
V. Compère ◽  
S. Li ◽  
J. Leprince ◽  
M.C. Tonon ◽  
H. Vaudry ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Mrna Levels ◽  
Corticotropin Releasing Hormone ◽  
Releasing Hormone

Ibuprofen augments bradykinin-induced glycoconjugate secretion by human nasal mucosa in vivo

1992 ◽  
Vol 89 (5) ◽  
pp. 1032-1039 ◽  
Author(s):  
J BARANIUK ◽  
P SILVER ◽  
M KALINER ◽  
P BARNES
Keyword(s):  
Nasal Mucosa ◽  
Human Nasal Mucosa

Permeation of polysucrose 15 000 across the human nasal mucosa in vivo

Allergy ◽  
1997 ◽  
Vol 52 (12) ◽  
pp. 1229-1232 ◽  
Author(s):  
M. Andersson ◽  
L. Greiff ◽  
H. Öman ◽  
C. Svensson ◽  
P. Wollmer ◽  
...  
Keyword(s):  
Nasal Mucosa ◽  
Human Nasal Mucosa

Predicting Risk of Preterm Birth: The Roles of Stress, Clinical Risk Factors, and Corticotropin-Releasing Hormone

2002 ◽  
Vol 4 (1) ◽  
pp. 54-64 ◽  
Author(s):  
R. Jeanne Ruiz ◽  
Judith Fullerton ◽  
Charles E. L. Brown ◽  
Donald J. Dudley
Keyword(s):  
Risk Factors ◽  
Preterm Birth ◽  
Perceived Stress ◽  
Gestational Age ◽  
Maternal Plasma ◽  
Clinical Risk Factors ◽  
Corticotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Clinical Risk ◽  
Prospective Longitudinal

The relationships and predictive abilities of perceived stress, selected clinical risk factors, and corticotropin-releasing hormone (CRH) levels in maternal plasma were investigated for their association with preterm labor (PTL), preterm birth, and gestational age at delivery. An exploratory, prospective, longitudinal research design was used to measure CRH 4 times over pregnancy, perceived stress at 24 and 32 weeks of pregnancy, clinical risk factors, and genitourinary infections in low-income women. Multiple regression analyses revealed that a model of measurement of perceived stress at 24 or 32 weeks, CRH at 24 or 32 weeks, and PTL (indicated by a diagnosis by the physicians on the medical record and greater than 5 contractions per hour on the fetal monitor) was predictive of as much as 0.23 to 0.27 of the variance in gestational age at birth. Entering ethnicity as a variable into a model did not improve the predictive value. An analysis of variance between Caucasian and Hispanic ethnic groups revealed differences in CRH levels. However, simple regression analysis of ethnicity as a predictor showed a weak r 2 with no significance for prediction. There was some evidence of an association of smoking with stress and both PTL and preterm birth. The measurement of stress combined with the measurement of CRH from maternal plasma may improve the prediction of which pregnant women are at risk for preterm birth. The measurement of CRH has potential as an early biological marker of preterm birth.

scholarly journals Effects of corticotropin-releasing hormone on locus coeruleus neurons in vivo: a microdialysis study using a novel bilateral approach

2001 ◽  
pp. 359-363 ◽  
Author(s):  
S Asbach ◽  
C Schulz ◽  
H Lehnert
Keyword(s):  
Locus Coeruleus ◽  
Fused Silica ◽  
Corticotropin Releasing Hormone ◽  
Release Of Noradrenaline ◽  
Releasing Hormone ◽  
Significant Prolongation ◽  
Bilateral Approach ◽  
Microdialysis Study ◽  
Na Release

OBJECTIVE: Stress-induced release of noradrenaline (NA) from locus coeruleus (LC) neurons is mainly regulated by corticotropin-releasing hormone (CRH). Tyrosine is a precursor of NA and plays an intriguing role in the regulation of NA release. DESIGN: We studied the effects of injecting CRH into the LC using a novel bilateral approach which relies on the mainly ipsilateral projections of LC neurons allowing stimulation of one hemisphere while using the other as control. To analyze the modification of the CRH effect, tyrosine was given intraperitoneally. A combination of CRH and its antagonist d-Phe was administered for validation of the specificity of CRH effects. METHODS: Wistar rats were used in all experiments. Injections were made through fused silica capillaries implanted into both LCs and microdialysis samples were collected bilaterally from the prefrontal cortex (PFM) every 20 min for 1 h before and 3 h after injections. The effects of LC stimulation were investigated by determining 3-methoxy-4-hydroxyphenylglycol (MHPG) in the dialysates. RESULTS: Following CRH injection into one LC and contralateral infusion of artificial cerebrospinal fluid (aCSF), MHPG levels, which are indicative of NA release, increased only in the ipsilateral PFM. These effects were blocked by d-Phe. Simultaneous administration of tyrosine i.p. led to a significant prolongation of MHPG release. CONCLUSIONS: These data provide the first physiological evidence of unilateral LC projections with the bilateral stimulation design proving to be a very valuable tool for the study of LC firing rate, to decrease number of animals and time expenditure. Prolongation of MHPG release after tyrosine supplementation is most likely due to increased NA synthesis.

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