Effects of Androgen Receptor Inhibition on Kanamycin-Induced Hearing Loss in Rats

Megalin has been proposed as an endocytic receptor for aminoglycosides as well as estrogen and androgen. We aimed to investigate the otoprotective effects of antiandrogens (flutamide, FM) on kanamycin (KM)-induced hearing loss in rats. Rats were divided into four groups. The KM group was administered KM (20 mg/kg/day) for 5 days, while the FM group received FM (15 mg/kg/day) for 10 days. In the KM + FM group, KM and FM (15 mg/kg/day) were simultaneously injected for 5 days and then FM was injected for 5 days. Auditory brainstem responses were measured. Western blotting and/or quantitative reverse transcriptase-polymerase chain reaction were performed for megalin, cytochrome P450 1A1 (Cyp1a1), Cyp1b1, metallothionein 1A (MT1A), MT2A, tumor necrosis factor (TNF)-α, caspase 3, and cleaved caspase 3. The FM + KM group showed attenuated auditory thresholds when compared with the KM group at 4, 8, 16, and 32 kHz (all p < 0.05). The KM + FM group showed lower megalin and Cyp1b1 levels than the KM group (all p < 0.05). The KM + FM group revealed lower MT1A, TNFα, and caspase 3 protein levels, compared with those in the KM group (all p < 0.05). Androgen receptor inhibition protects against cochlear injuries in KM-induced hearing loss rats by attenuating megalin expression, revealing anti-inflammatory and anti-apoptotic effects.

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Applicability of Evoked Auditory Brainstem Responses with Complex Stimuli in Adults with Hearing Loss

International Archives of Otorhinolaryngology ◽

10.1055/s-0037-1605341 ◽

2017 ◽

Vol 22 (03) ◽

pp. 239-244 ◽

Cited By ~ 1

Author(s):

Bruna Peixe ◽

Débora Silva ◽

Eliara Biaggio ◽

Rúbia Bruno ◽

Taissane Sanguebuche ◽

...

Keyword(s):

Hearing Loss ◽

Auditory Brainstem ◽

Auditory Brainstem Responses ◽

Study Group ◽

Auditory Thresholds ◽

High Frequencies ◽

Complex Stimuli ◽

Brainstem Response ◽

Normal Standards ◽

Complex Manner

Introduction The use of the speech-evoked auditory brainstem response (ABR) shows how the brainstem operates up to the subcortex in a more complex manner than when the click-evoked ABR is used. Objective To study the applicability of the speech-evoked ABR in adults with hearing loss. Methods The sample was composed of a study group of 11 subjects, with ages ranging between 18 and 59 years, and auditory thresholds within normal standards, with loss of up to 65 dB at high frequencies or up to moderately severe symmetric sensorineural hearing loss. The sample underwent a basic audiological assessment, as well as speech-evoked ABR and click-evoked ABR, in which waves I, III and V, and V, A, C, D, E, F were respectively marked. The electrophysiological assessments were performed using the SmartEP device (Intelligent Hearing Systems, Miami, FL, US). Results For the speech-evoked ABR, the reference values were used in the identification and analysis of the study group. Those values found for the study group were: V = 8.56; A = 10.97; C = 21.33; D = 29.51; E = 37.93; F = 46.96; and O = 55.97. In the comparison between groups, the study group presented an increase in latency only in wave C. Conclusion The speech-evoked ABR can be performed in subjects with up to moderately severe hearing loss, and the test proved to be appropriate, because, unlike the click-evoked ABR, the former does not suffer influence of peripheral hearing loss.

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Effects of Presbycusis and Other Types of Hearing Loss on Auditory Brainstem Responses

International Journal of Audiology ◽

10.3109/14992028609042141 ◽

1986 ◽

Vol 15 (4) ◽

pp. 179-185

Author(s):

Ulf Rosenhall ◽

Kai Pedersen ◽

Mats Dotevall

Keyword(s):

Hearing Loss ◽

Auditory Brainstem ◽

Auditory Brainstem Responses

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Hearing recovery from deafness caused by bromate intoxication

The Journal of Laryngology & Otology ◽

10.1017/s0022215118002001 ◽

2018 ◽

Vol 132 (11) ◽

pp. 1039-1041 ◽

Cited By ~ 1

Author(s):

J Suzuki ◽

Y Takanashi ◽

A Koyama ◽

Y Katori

Keyword(s):

Hearing Loss ◽

Sensorineural Hearing Loss ◽

Pure Tone ◽

Pure Tone Audiometry ◽

Auditory Brainstem ◽

Sensorineural Hearing ◽

Auditory Brainstem Responses ◽

Common Symptom ◽

Hearing Recovery ◽

Continuous Haemodiafiltration

AbstractObjectivesSodium bromate is a strong oxidant, and bromate intoxication can cause irreversible severe-to-profound sensorineural hearing loss. This paper reports the first case in the English literature of bromate-induced hearing loss with hearing recovery measured by formal audiological assessment.Case reportA 72-year-old woman was admitted to hospital with complaints of profound hearing loss, nausea, diarrhoea and anuria after bromate ingestion in a suicide attempt. On admission, pure tone audiometry and auditory brainstem responses showed profound bilateral deafness. Under the diagnosis of bromate-induced acute renal failure and sensorineural hearing loss, continuous haemodiafiltration was performed. When dialysis was discontinued, pure tone audiometry and auditory brainstem responses showed partial threshold recovery from profound deafness.ConclusionSevere-to-profound sensorineural hearing loss is a common symptom of bromate intoxication. Bromate-induced hearing loss may be partially treated, and early application of continuous haemodiafiltration might be useful as a treatment for this intractable condition.

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Auditory Brainstem Responses in a Case of High-Frequency Conductive Hearing Loss

Journal of Speech and Hearing Disorders ◽

10.1044/jshd.5004.346 ◽

1985 ◽

Vol 50 (4) ◽

pp. 346-350 ◽

Cited By ~ 8

Author(s):

Michael P. Gorga ◽

Jan K. Reiland ◽

Kathryn A. Beauchaine

Keyword(s):

Hearing Loss ◽

High Frequency ◽

Conductive Hearing Loss ◽

Auditory Brainstem ◽

Intensity Function ◽

Auditory Brainstem Responses ◽

Normal Limits ◽

Interpeak Latency ◽

Lower Limits ◽

Conductive Hearing

Click-evoked auditory brainstem responses were measured in a patient with high-frequency conductive hearing loss. As is typical in cases of conductive hearing loss, Wave I latency was prolonged beyond normal limits. Interpeak latency differences were just below the lower limits of the normal range. The Wave V latency-intensity function, however was abnormally steep. This pattern is explained by the hypothesis that the slope of the latency-intensity function is determined principally by the configuration of the hearing loss. In cases of high-frequency hearing loss (regardless of the etiology), the response may be dominated by more apical regions of the cochlea at lower intensities and thus have a longer latency.

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Comparison of auditory steady-state responses and auditory brainstem responses in audiometric assessment of adults with sensorineural hearing loss

Auris Nasus Larynx ◽

10.1016/j.anl.2008.04.009 ◽

2009 ◽

Vol 36 (2) ◽

pp. 140-145 ◽

Cited By ~ 27

Author(s):

Yu-Hsing Lin ◽

Hsu-Chueh Ho ◽

Hung-Pin Wu

Keyword(s):

Hearing Loss ◽

Steady State ◽

Sensorineural Hearing Loss ◽

Auditory Brainstem ◽

Sensorineural Hearing ◽

Auditory Brainstem Responses ◽

Steady State Responses ◽

Auditory Steady State Responses ◽

State Responses

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Otoprotective Effects of Zingerone on Cisplatin-Induced Ototoxicity

International Journal of Molecular Sciences ◽

10.3390/ijms21103503 ◽

2020 ◽

Vol 21 (10) ◽

pp. 3503 ◽

Cited By ~ 2

Author(s):

Chang Ho Lee ◽

Da-hye Lee ◽

So Min Lee ◽

So Young Kim

Keyword(s):

Caspase 3 ◽

Auditory Brainstem ◽

Control Group ◽

Heme Oxygenase 1 ◽

Sprague Dawley ◽

Necrosis Factor Alpha ◽

Auditory Thresholds ◽

Expression Levels ◽

Brainstem Response ◽

Group A

Previous studies have described the effects of zingerone (ZO) on cisplatin (CXP)-induced injury to the kidneys, liver, and other organs but not to the cochlea. This study aimed to investigate the effects of ZO on CXP-induced ototoxicity. Eight-week-old Sprague–Dawley rats were used and divided into a control group, a CXP group, and a CXP + ZO group. Rats in the CXP group received 5 mg/kg/day CXP intraperitoneally for five days. Rats in the CXP + ZO group received 5 mg/kg/day CXP intraperitoneally for five days and 50 mg/kg/day ZO intraperitoneally for seven days. Auditory brainstem response thresholds (ABRTs) were measured before (day 0) and after (day 10) drug administration. Cochlear histology was examined using hematoxylin and eosin (H&E) staining and cochlear whole mounts. The expression levels of cytochrome P450 (CYP)1A1, CYP1B1, inducible nitric oxide synthase (iNOS), nuclear factor kappa B (NFκB), tumor necrosis factor alpha (TNFα), and interleukin 6 (IL6) were estimated using quantitative reverse transcription-polymerase chain reaction. The expression levels of heme oxygenase 1 (HO1) and caspase 3 were analyzed via Western blotting. The auditory thresholds at 4, 8, and 16 kHz were attenuated in the CXP + ZO group compared with the CXP group. The mRNA expression levels of CYP1A1, CYP1B1, iNOS, NFκB, TNFα, and IL6 were lower in the CXP + ZO group than in the CXP group. The protein expression levels of HO1 and caspase 3 were lower in the CXP + ZO group than in the CXP group. Cotreatment with ZO exerted otoprotective effects against CXP-induced cochlear injury via antioxidative and anti-inflammatory activities involving CYPs, iNOS, NFκB, and TNFα.

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