Comparative Study of the Genetic and Biochemical Variability of Polyscias filicifolia (Araliaceae) Regenerants Obtained by Indirect and Direct Somatic Embryogenesis as a Source of Triterpenes

Polyscias filicifolia (Araliaceae) is broadly used in traditional medicine in Southeast Asia due to its antimicrobial, immunomodulating and cytotoxic activities. The main groups of compounds responsible for pharmacological effects are believed to be oleanolic triterpene saponins. However, Polyscias plants demonstrate relatively slow growth in natural conditions, which led to applying a developing sustainable source of plant material via primary (PSE), secondary (DSE) and direct somatic embryogenesis from DSE (TSE). The AFLP and metAFLP genotyping resulted in 1277 markers, amplified by a total of 24 pairs of selective primers. Only 3.13% of the markers were polymorphic. The analysis of variance showed that the PSE and TSE regenerants differed only in terms of root number, while the DSE plantlets differed for all studied morphological characteristics. Further, the chemical analysis revealed that oleanolic acid (439.72 µg/g DW), ursolic acid (111.85 µg/g DW) and hederagenin (19.07 µg/g DW) were determined in TSE regenerants. Our results indicate that direct somatic embryogenesis ensures the production of homogeneous plant material, which can serve as a potential source of triterpene compounds. Plants obtained via somatic embryogenesis could also be reintroduced into the natural environment to protect and preserve its biodiversity.

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Erratum to: Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-016-9777-2 ◽

2016 ◽

Vol 52 (5) ◽

pp. 520-520

Author(s):

Chayanika Bhattacharya ◽

Anandamoy Dam ◽

Joydeep Karmakar ◽

Tapas Kumar Bandyopadhyay

Keyword(s):

Somatic Embryogenesis ◽

Genetic Homogeneity ◽

Direct Somatic Embryogenesis ◽

Regenerated Plants ◽

Homogeneity Assessment ◽

Anthurium Andraeanum

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Influence of Abscisic Acid and Sucrose on Somatic Embryogenesis in CactusCopiapoa tenuissimaRitt. formamostruosa

The Scientific World JOURNAL ◽

10.1155/2013/513985 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 13

Author(s):

J. Lema-Rumińska ◽

K. Goncerzewicz ◽

M. Gabriel

Keyword(s):

Somatic Embryogenesis ◽

Abscisic Acid ◽

Somatic Embryos ◽

Sucrose Concentration ◽

Turgor Pressure ◽

Direct Somatic Embryogenesis ◽

Fresh Weight ◽

High Concentration ◽

Globular Stage ◽

Indirect Somatic Embryogenesis

Having produced the embryos of cactusCopiapoa tenuissimaRitt. formamonstruosaat the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μM on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA (0, 0.1, 1 μM) and sucrose (1, 3, 5%). The results showed that a low concentration of ABA (0-1 μM) stimulates the elongation of embryos at the globular stage and the number of correct embryos in direct somatic embryogenesis, while a high ABA concentration (10–100 μM) results in growth inhibition and turgor pressure loss of somatic embryos. The indirect somatic embryogenesis study in this cactus suggests that lower ABA concentrations enhance the increase in calli fresh weight, while a high concentration of 10 μM ABA or more changes calli color and decreases its proliferation rate. However, in the case of indirect somatic embryogenesis, ABA had no effect on the number of somatic embryos and their maturation. Nevertheless, we found a positive effect of sucrose concentration for both the number of somatic embryos and the increase in calli fresh weight.

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Organogenesis, direct somatic embryogenesis, and shoot proliferation of Rheum spiciforme Royle: an endemic and vulnerable medicinal herb from Indian Trans Himalayas

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-021-10211-2 ◽

2021 ◽

Author(s):

Mohd Ishfaq Khan ◽

Anwar Shahzad ◽

Irfan Bashir Ganie ◽

Shahzad A. Pandith

Keyword(s):

Somatic Embryogenesis ◽

Shoot Proliferation ◽

Medicinal Herb ◽

Direct Somatic Embryogenesis

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Production ofAgrobacterium-mediated transgenic fertile plants by direct somatic embryogenesis from immature zygotic embryos ofDatura innoxia

Plant Molecular Biology ◽

10.1007/bf00014673 ◽

1994 ◽

Vol 25 (6) ◽

pp. 995-1009 ◽

Cited By ~ 14

Author(s):

Corinne Ducrocq ◽

Rajbir S. Sangwan ◽

Brigitte S. Sangwan-Norreel

Keyword(s):

Somatic Embryogenesis ◽

Direct Somatic Embryogenesis ◽

Zygotic Embryos ◽

Immature Zygotic Embryos

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The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v27i2.146 ◽

2011 ◽

Vol 27 (2) ◽

Author(s):

Rina Arimarsetiowati

Keyword(s):

Tissue Culture ◽

Somatic Embryogenesis ◽

Coffea Arabica ◽

Callus Formation ◽

Culture Technique ◽

Plant Production ◽

Direct Somatic Embryogenesis ◽

Arabica Coffee ◽

Randomized Design ◽

Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

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The Effect of Slow-grown in Vitro Storage Under Different Light Spectra on Banana Plantlets Cv. Prata Catarina (AAB).

10.21203/rs.3.rs-597268/v1 ◽

2021 ◽

Author(s):

Paulo Hercilio Viegas Rodrigues ◽

Emerson Oliveira ◽

Christian Demetrio ◽

Guilherme Ambrosano ◽

Sônia Maria Stefano Piedade

Keyword(s):

Plant Material ◽

Slow Growth ◽

Shoot Cultures ◽

Ms Medium ◽

In Vitro Storage ◽

Light Spectra ◽

Slow Growth Storage ◽

C 50 ◽

Commercial Micropropagation

Abstract Maintaining updated in vitro plant subcultures is essential for commercial micropropagation and tissue culture research. In unusual situations, the subcultures can be delay and the slow-growth in vitro storage technic could be applied to reduce the loss of plant material. The present study aimed to evaluate the slow-growth in vitro storage of banana plantlets (‘Prata Catarina’; group AAB) under different light spectra. Shoot cultures in MS medium without plant growth regulators were maintained under blue (B), red (R), red plus blue (R2B), and white (CW) light spectra (25°C ± 2°C; 50 µmol m -2 s -1 ) for up to 140 days. The plantlets maintained under the R, CW, and R2B spectra did not survive after 140 days of in vitro slow-growth storage. The plantlets maintained under the B spectrum survived after 140 days of in vitro slow-growth storage and showed little browning.

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The Chemical Environment at Maturation Stage in Pinus spp. Somatic Embryogenesis: Implications in the Polyamine Profile of Somatic Embryos and Morphological Characteristics of the Developed Plantlets

Frontiers in Plant Science ◽

10.3389/fpls.2021.771464 ◽

2021 ◽

Vol 12 ◽

Author(s):

Antonia Maiara Marques do Nascimento ◽

Luiza Giacomolli Polesi ◽

Franklin Panato Back ◽

Neusa Steiner ◽

Miguel Pedro Guerra ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Amino Acid ◽

Somatic Embryos ◽

Pinus Halepensis ◽

Morphological Characteristics ◽

Chemical Environment ◽

Acid Mixture ◽

Maturation Stage ◽

Maturation Medium ◽

Pinus Spp

Changes in the chemical environment at the maturation stage in Pinus spp. somatic embryogenesis will be a determinant factor in the conversion of somatic embryos to plantlets. Furthermore, the study of biochemical and morphological aspects of the somatic embryos could enable the improvement of somatic embryogenesis in Pinus spp. In the present work, the influence of different amino acid combinations, carbohydrate sources, and concentrations at the maturation stage of Pinus radiata D. Don and Pinus halepensis Mill. was analyzed. In P. radiata, the maturation medium supplemented with 175 mM of sucrose and an increase in the amino acid mixture (1,100 mgL–1 of L-glutamine, 1,050 mgL–1 of L-asparagine, 350 mgL–1 of L-arginine, and 35 mgL–1 of L-proline) promoted bigger embryos, with a larger stem diameter and an increase in the number of roots in the germinated somatic embryos, improving the acclimatization success of this species. In P. halepensis, the maturation medium supplemented with 175 mM of maltose improved the germination of somatic embryos. The increase in the amount of amino acids in the maturation medium increased the levels of putrescine in the germinated somatic embryos of P. halepensis. We detected significant differences in the amounts of polyamines between somatic plantlets of P. radiata and P. halepensis; putrescine was less abundant in both species. For the first time, in P. radiata and P. halepensis somatic embryogenesis, we detected the presence of cadaverine, and its concentration changed according to the species.

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