scholarly journals Erratum: Tran et al. Early Cellular Responses Induced by Sedimentary Calcite-Processed Particles in Bright Yellow 2 Tobacco Cultured Cells. Int. J. Mol. Sci. 2020, 21, 4279

2021 ◽  
Vol 22 (13) ◽  
pp. 6863
Author(s):  
Daniel Tran ◽  
Tingting Zhao ◽  
Delphine Arbelet-Bonnin ◽  
Takashi Kadono ◽  
Patrice Meimoun ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Cellular Responses ◽  
Bright Yellow ◽  
Published Paper

The authors would like to remove the scientific consortium ‘Camille Nous’ from the author list and the Author Contributions section in the published paper [...]

scholarly journals Early Cellular Responses Induced by Sedimentary Calcite-Processed Particles in Bright Yellow 2 Tobacco Cultured Cells

2020 ◽  
Vol 21 (12) ◽  
pp. 4279 ◽  
Author(s):  
Daniel Tran ◽  
Tingting Zhao ◽  
Delphine Arbelet-Bonnin ◽  
Takashi Kadono ◽  
Patrice Meimoun ◽  
...  
Keyword(s):  
Co2 Fixation ◽  
Cultured Cells ◽  
Cellular Responses ◽  
Ros Generation ◽  
Oxygen Species ◽  
Anion Channels ◽  
Cellular Events ◽  
Bright Yellow ◽  
Limestone Rock ◽  
Micrometer Size

Calcite processed particles (CaPPs, Megagreen®) elaborated from sedimentary limestone rock, and finned by tribomecanic process were found to increase photosynthetic CO2 fixation grapevines and stimulate growth of various cultured plants. Due to their processing, the CaPPs present a jagged shape with some invaginations below the micrometer size. We hypothesised that CaPPs could have a nanoparticle (NP)-like effects on plants. Our data show that CaPPs spontaneously induced reactive oxygen species (ROS) in liquid medium. These ROS could in turn induce well-known cellular events such as increase in cytosolic Ca2+, biotic ROS generation and activation of anion channels indicating that these CaPPs could activate various signalling pathways in a NP-like manner.

scholarly journals Complex Proteolytic Processing Acts on Delta, a Transmembrane Ligand for Notch, during Drosophila Development

1998 ◽  
Vol 9 (7) ◽  
pp. 1709-1723 ◽  
Author(s):  
Kristin M. Klueg ◽  
Todd R. Parody ◽  
Marc A.T. Muskavitch
Keyword(s):  
Cell Fate ◽  
Cultured Cells ◽  
Cellular Responses ◽  
Proteolytic Processing ◽  
Cell Fate Specification ◽  
A Cell ◽  
Membrane Bound ◽  
Delta Functions ◽  
Intracellular Domains

Delta functions as a cell nonautonomous membrane-bound ligand that binds to Notch, a cell-autonomous receptor, during cell fate specification. Interaction between Delta and Notch leads to signal transduction and elicitation of cellular responses. During our investigations to further understand the biochemical mechanism by which Delta signaling is regulated, we have identified four Delta isoforms inDrosophila embryonic and larval extracts. We have demonstrated that at least one of the smaller isoforms, Delta S, results from proteolysis. Using antibodies to the Delta extracellular and intracellular domains in colocalization experiments, we have found that at least three Delta isoforms exist in vivo, providing the first evidence that multiple forms of Delta exist during development. Finally, we demonstrate that Delta is a transmembrane ligand that can be taken up by Notch-expressing Drosophila cultured cells. Cell culture experiments imply that full-length Delta is taken up by Notch-expressing cells. We present evidence that suggests this uptake occurs by a nonphagocytic mechanism.

scholarly journals Genetic Analysis of the Drosophila Gsα Gene

Genetics ◽  
2001 ◽  
Vol 158 (3) ◽  
pp. 1189-1201 ◽  
Author(s):  
William J Wolfgang ◽  
Ashwini Hoskote ◽  
Ian J H Roberts ◽  
Shannon Jackson ◽  
Michael Forte
Keyword(s):  
Cultured Cells ◽  
Protein Complexes ◽  
Cellular Responses ◽  
Signal Transduction Pathways ◽  
Intracellular Camp ◽  
Loss Of Function ◽  
Wild Type ◽  
Gsα Gene ◽  
Developmental Contexts ◽  
Stimulation Of

Abstract One of the best understood signal transduction pathways activated by receptors containing seven transmembrane domains involves activation of heterotrimeric G-protein complexes containing Gsα, the subsequent stimulation of adenylyl cyclase, production of cAMP, activation of protein kinase A (PKA), and the phosphorylation of substrates that control a wide variety of cellular responses. Here, we report the identification of “loss-of-function” mutations in the Drosophila Gsα gene (dgs). Seven mutants have been identified that are either complemented by transgenes representing the wild-type dgs gene or contain nucleotide sequence changes resulting in the production of altered Gsα protein. Examination of mutant alleles representing loss-of-Gsα function indicates that the phenotypes generated do not mimic those created by mutational elimination of PKA. These results are consistent with the conclusion reached in previous studies that activation of PKA, at least in these developmental contexts, does not depend on receptor-mediated increases in intracellular cAMP, in contrast to the predictions of models developed primarily on the basis of studies in cultured cells.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

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