scholarly journals Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process

2021 ◽  
Vol 22 (14) ◽  
pp. 7331
Author(s):  
Justyna Szczykutowicz ◽  
Joanna Tkaczuk-Włach ◽  
Mirosława Ferens-Sieczkowska
Keyword(s):  
Immune Response ◽  
Seminal Plasma ◽  
Immune Modulation ◽  
Lectin Affinity Chromatography ◽  
Human Seminal Plasma ◽  
Plant Lectins ◽  
Healthy Pregnancy ◽  
Endogenous Lectins ◽  
Maternal Immune Response ◽  
Inducible Protein

In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy. Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells. Such interaction may be involved in modulation of the maternal immune response. Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding. In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers. By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive. Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable. STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes. Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.

Human serum albumin as a new interacting partner of prolactin inducible protein in human seminal plasma

2012 ◽  
Vol 50 (2) ◽  
pp. 317-322 ◽  
Author(s):  
Sanjay Kumar ◽  
Anil Kumar Tomar ◽  
Sudhuman Singh ◽  
Mayank Saraswat ◽  
Sarman Singh ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Seminal Plasma ◽  
Human Seminal Plasma ◽  
Inducible Protein

Quantification studies in human seminal plasma samples identify prolactin inducible protein as a plausible marker of azoospermia

Biomarkers ◽  
2012 ◽  
Vol 17 (6) ◽  
pp. 545-551 ◽  
Author(s):  
Anil Kumar Tomar ◽  
Balwinder Singh Sooch ◽  
Sarman Singh ◽  
Savita Yadav
Keyword(s):  
Seminal Plasma ◽  
Plasma Samples ◽  
Human Seminal Plasma ◽  
Inducible Protein

scholarly journals Maternal Serum Cytokine Concentrations in Healthy Pregnancy and Preeclampsia

2021 ◽  
Vol 2021 ◽  
pp. 1-33
Author(s):  
Toni Spence ◽  
Philip J. Allsopp ◽  
Alison J. Yeates ◽  
Maria S. Mulhern ◽  
J. J. Strain ◽  
...  
Keyword(s):  
Immune Response ◽  
Maternal Serum ◽  
T Cell Subsets ◽  
Second Trimester ◽  
Limited Information ◽  
Serum Cytokine ◽  
Healthy Pregnancy ◽  
Tnf Α ◽  
Maternal Immune Response ◽  
In Pregnancy

The maternal immune response is essential for successful pregnancy, promoting immune tolerance to the fetus while maintaining innate and adaptive immunity. Uncontrolled, increased proinflammatory responses are a contributing factor to the pathogenesis of preeclampsia. The Th1/Th2 cytokine shift theory, characterised by bias production of Th2 anti-inflammatory cytokine midgestation, was frequently used to reflect the maternal immune response in pregnancy. This theory is simplistic as it is based on limited information and does not consider the role of other T cell subsets, Th17 and Tregs. A range of maternal peripheral cytokines have been measured in pregnancy cohorts, albeit the changes in individual cytokine concentrations across gestation is not well summarised. Using available data, this review was aimed at summarising changes in individual maternal serum cytokine concentrations throughout healthy pregnancy and evaluating their association with preeclampsia. We report that TNF-α increases as pregnancy progresses, IL-8 decreases in the second trimester, and IL-4 concentrations remain consistent throughout gestation. Lower second trimester IL-10 concentrations may be an early predictor for developing preeclampsia. Proinflammatory cytokines (TNF-α, IFN-γ, IL-2, IL-8, and IL-6) are significantly elevated in preeclampsia. More research is required to determine the usefulness of using cytokines, particularly IL-10, as early biomarkers of pregnancy health.

Seminal plasma peptides may determine maternal immune response that alters success or failure of pregnancy in the abortion-prone CBAxDBA/2 model

2013 ◽  
Vol 99 (1-2) ◽  
pp. 46-53 ◽  
Author(s):  
David A. Clark ◽  
Mona Rahmati ◽  
Claudia Gohner ◽  
Armand Bensussan ◽  
Udo R. Markert ◽  
...  
Keyword(s):  
Immune Response ◽  
Seminal Plasma ◽  
Maternal Immune Response

scholarly journals V-ATPase upregulation during early pregnancy: a possible link to establishment of an inflammatory response during preimplantation period of pregnancy

Reproduction ◽  
2012 ◽  
Vol 143 (5) ◽  
pp. 713-725 ◽  
Author(s):  
Mukesh K Jaiswal ◽  
Timothy M Mallers ◽  
Benjamin Larsen ◽  
Joanne Kwak-Kim ◽  
Gerard Chaouat ◽  
...  
Keyword(s):  
Immune Response ◽  
Seminal Plasma ◽  
Preimplantation Embryos ◽  
Polarization Ratio ◽  
Successful Pregnancy ◽  
Chemotactic Protein ◽  
Maternal Immune Response ◽  
Anti Inflammatory ◽  
Inflammatory Milieu ◽  
Inflammatory Macrophages

Various mechanisms exist to prevent a potentially deleterious maternal immune response that results in compromising survival of semiallogeneic fetus. In pregnancy, there is a necessary early preimplantation inflammatory stage followed by a postimplantation anti-inflammatory stage. Thus, there is a biphasic ‘immune response’ observed during the course of pregnancy. We provide the evidence that capacitation of sperm induced the expression of a2 isoform of V-ATPase (ATP6V0A2 referred to as a2V), leukemia inhibitory factor (Lif),Il1b, andTnfin the sperm. Capacitated sperm also released cleaved N-terminal domain of a2V-ATPase (a2NTD), which upregulates the gene expression ofLif,Il1b,Tnf, and monocyte chemotactic protein-1 (Ccl2(Mcp1)) in the uterus. Unfertilized eggs had low a2V expression, but after fertilization, the expression of a2V increased in zygotes. This increased level of a2V expression was maintained in preimplantation embryos. Seminal plasma was necessary for upregulation of a2V expression in preimplantation embryos, as mating with seminal vesicle-deficient males failed to elicit an increase in a2V expression in preimplantation embryos. The infiltration of macrophages into the uterus was significantly increased after insemination of both sperm and seminal plasma during the preimplantation period of pregnancy. This dynamic infiltration into the uterus corresponded with the uterine a2V expression through the induction ofCcl2expression. Furthermore, the polarization ratio of M1:M2 (pro-inflammatory/anti-inflammatory) macrophages in the uterus fluctuated from a ratio of 1.60 (day 1) to 1.45 (day 4) when female mice were inseminated with both sperm and seminal plasma. These data provide evidence that exposure to semen may initiate an inflammatory milieu by inducing a2V and cytokine/chemokine expression, which triggers the influx of macrophages into the preimplantation uterus during the onset of pregnancy and ultimately leads to successful pregnancy outcome.

Crystal Structure of the Novel Complex Formed between Zinc α2-Glycoprotein (ZAG) and Prolactin-Inducible Protein (PIP) from Human Seminal Plasma

2008 ◽  
Vol 384 (3) ◽  
pp. 663-672 ◽  
Author(s):  
Md. Imtaiyaz Hassan ◽  
Sameeta Bilgrami ◽  
Vijay Kumar ◽  
Nagendra Singh ◽  
Savita Yadav ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Seminal Plasma ◽  
The Novel ◽  
Human Seminal Plasma ◽  
Novel Complex ◽  
Inducible Protein
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