scholarly journals Identification of FadT as a Novel Quorum Quenching Enzyme for the Degradation of Diffusible Signal Factor in Cupriavidus pinatubonensis Strain HN-2

2021 ◽  
Vol 22 (18) ◽  
pp. 9862
Author(s):  
Xudan Xu ◽  
Tian Ye ◽  
Wenping Zhang ◽  
Tian Zhou ◽  
Xiaofan Zhou ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Xanthomonas Campestris ◽  
Cell Communication ◽  
Maximal Activity ◽  
Quorum Quenching ◽  
Site Directed Mutagenesis ◽  
Diffusible Signal Factor ◽  
High Enzymatic Activity ◽  
Xanthomonas Campestris Pv Campestris ◽  
Potential Use

Quorum sensing (QS) is a microbial cell–cell communication mechanism and plays an important role in bacterial infections. QS-mediated bacterial infections can be blocked through quorum quenching (QQ), which hampers signal accumulation, recognition, and communication. The pathogenicity of numerous bacteria, including Xanthomonas campestris pv. campestris (Xcc), is regulated by diffusible signal factor (DSF), a well-known fatty acid signaling molecule of QS. Cupriavidus pinatubonensis HN-2 could substantially attenuate the infection of XCC through QQ by degrading DSF. The QQ mechanism in strain HN-2, on the other hand, is yet to be known. To understand the molecular mechanism of QQ in strain HN-2, we used whole-genome sequencing and comparative genomics studies. We discovered that the fadT gene encodes acyl-CoA dehydrogenase as a novel QQ enzyme. The results of site-directed mutagenesis demonstrated the requirement of fadT gene for DSF degradation in strain HN-2. Purified FadT exhibited high enzymatic activity and outstanding stability over a broad pH and temperature range with maximal activity at pH 7.0 and 35 °C. No cofactors were required for FadT enzyme activity. The enzyme showed a strong ability to degrade DSF. Furthermore, the expression of fadT in Xcc results in a significant reduction in the pathogenicity in host plants, such as Chinese cabbage, radish, and pakchoi. Taken together, our results identified a novel DSF-degrading enzyme, FadT, in C. pinatubonensis HN-2, which suggests its potential use in the biological control of DSF-mediated pathogens.

scholarly journals Characterization of a Novel Quorum-Quenching Bacterial Strain, Burkholderia anthina HN-8, and Its Biocontrol Potential against Black Rot Disease Caused by Xanthomonas campestris pv. campestris

2020 ◽  
Vol 8 (10) ◽  
pp. 1485
Author(s):  
Tian Ye ◽  
Wenping Zhang ◽  
Zhixuan Feng ◽  
Xinghui Fan ◽  
Xudan Xu ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Xanthomonas Campestris ◽  
Bacterial Pathogens ◽  
Quorum Quenching ◽  
Gas Chromatography Mass Spectrometry ◽  
Black Rot ◽  
Degrading Bacterium ◽  
Diffusible Signal Factor ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris

Diffusible signal factor (DSF) is a type of cis unsaturated fatty acid, with a chemical structure of 11-methyl-2-dodecylene acid. DSF is widely conserved in a variety of Gram-negative bacterial pathogens and is involved in the regulation of pathogenic virulence. Quorum quenching (QQ) is a promising strategy for preventing and controlling quorum sensing (QS)-mediated bacterial infections by interfering with the QS system of pathogens. In this study, a novel DSF-degrading bacterium, Burkholderia anthina strain HN-8, was isolated and characterized for its degradation ability and potential biocontrol of black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). The HN-8 strain exhibited superb DSF degradation activity and completely degraded 2 mM DSF within 48 h. In addition, we present the first evidence of bacterium having a metabolic pathway for the complete degradation and metabolism of DSF. Analysis of DSF metabolic products by gas chromatography–mass spectrometry led to the identification of dodecanal as the main intermediate product, revealing that DSF could be degraded via oxidation–reduction. Furthermore, application of strain HN-8 as a potent biocontrol agent was able to significantly reduce the severity of black rot disease in radishes and Chinese cabbage. Taken together, these results shed light on the QQ mechanisms of DSF, and they provide useful information showing the potential for the biocontrol of infectious diseases caused by DSF-dependent bacterial pathogens.

The cis -2-dodecenoic acid (BDSF) quorum sensing system in Burkholderia cenocepacia

2022 ◽  
Author(s):  
Mingfang Wang ◽  
Xia Li ◽  
Shihao Song ◽  
Chaoyu Cui ◽  
Lian-Hui Zhang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Bacterial Infections ◽  
Xanthomonas Campestris ◽  
Cell Communication ◽  
Burkholderia Cenocepacia ◽  
Bacterial Cells ◽  
Communication Signals ◽  
Signal Perception ◽  
Sensing System ◽  
Diffusible Signal Factor

It has been demonstrated that quorum sensing (QS) is widely employed by bacterial cells to coordinately regulate various group behaviors. Diffusible signal factor (DSF)-type signals have emerged as a growing family of conserved cell-cell communication signals. In addition to the DSF signal initially identified in Xanthomonas campestris pv. campestris, B urkholderia d iffusible s ignal f actor (BDSF, cis -2-dodecenoic acid) has been recognized as a conserved DSF-type signal with specific characteristics in both signal perception and transduction from DSF signals. Here, we review the history and current progress of the research of this type of signal, especially focusing on its biosynthesis, signaling pathways, and biological functions. We also discuss and explore the huge potential of targeting this kind of QS system as a new therapeutic strategy to control bacterial infections and diseases.

scholarly journals Cupriavidus sp. HN-2, a Novel Quorum Quenching Bacterial Isolate, is a Potent Biocontrol Agent Against Xanthomonas campestris pv. campestris

2019 ◽  
Vol 8 (1) ◽  
pp. 45 ◽  
Author(s):  
Tian Ye ◽  
Tian Zhou ◽  
Qiting Li ◽  
Xudan Xu ◽  
Xinghui Fan ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Xanthomonas Campestris ◽  
Biocontrol Agent ◽  
Bacterial Isolate ◽  
Bacterial Pathogens ◽  
Acid Methyl Ester ◽  
Quorum Quenching ◽  
Gas Chromatography Mass Spectrometry ◽  
Biochemical Basis ◽  
Xanthomonas Campestris Pv Campestris

Diffusible signal factor (DSF) represents a family of widely conserved quorum sensing (QS) signals involved in the regulation of virulence factor production in many Gram-negative bacterial pathogens. Quorum quenching, which disrupts QS either by degradation of QS signals or interference of signal generation or perception, is a promising strategy for prevention and control of QS-mediated bacterial infections. In this study, a novel DSF-degrading strain, HN-2, was isolated from contaminated soil and identified as Cupriavidus sp. The isolate exhibited superior DSF degradation activity and completely degraded 2 mmol·L–1 of DSF within 24 h. Analysis of the degradation products of DSF by gas chromatography–mass spectrometry led to the identification of trans-2-decenoic acid methyl ester as the main intermediate product, suggesting that DSF could be degraded by oxidation and hydroxylation. Moreover, this study presents for the first time, evidence that Cupriavidus sp. can reduce the black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). Application of the HN-2 strain as a biocontrol agent could substantially reduce the disease severity. These findings reveal the biochemical basis of a highly efficient DSF-degrading bacterial isolate and present a useful agent for controlling infectious diseases caused by DSF-dependent bacterial pathogens.

scholarly journals Whole-Genome Sequencing Analysis of Quorum Quenching Bacterial Strain Acinetobacter lactucae QL-1 Identifies the FadY Enzyme for Degradation of the Diffusible Signal Factor

2020 ◽  
Vol 21 (18) ◽  
pp. 6729 ◽  
Author(s):  
Tian Ye ◽  
Tian Zhou ◽  
Xudan Xu ◽  
Wenping Zhang ◽  
Xinghui Fan ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Xanthomonas Campestris ◽  
Molecular Mechanisms ◽  
Bacterial Pathogens ◽  
Quorum Quenching ◽  
Site Directed Mutagenesis ◽  
Whole Genome ◽  
Sequencing Analysis ◽  
Diffusible Signal Factor

The diffusible signal factor (DSF) is a fatty acid signal molecule and is widely conserved in various Gram-negative bacteria. DSF is involved in the regulation of pathogenic virulence in many bacterial pathogens, including Xanthomonas campestris pv. campestris (Xcc). Quorum quenching (QQ) is a potential approach for preventing and controlling DSF-mediated bacterial infections by the degradation of the DSF signal. Acinetobacter lactucae strain QL-1 possesses a superb DSF degradation ability and effectively attenuates Xcc virulence through QQ. However, the QQ mechanisms in strain QL-1 are still unknown. In the present study, whole-genome sequencing and comparative genomics analysis were conducted to identify the molecular mechanisms of QQ in strain QL-1. We found that the fadY gene of QL-1 is an ortholog of XccrpfB, a known DSF degradation gene, suggesting that strain QL-1 is capable of inactivating DSF by QQ enzymes. The results of site-directed mutagenesis indicated that fadY is required for strain QL-1 to degrade DSF. The determination of FadY activity in vitro revealed that the fatty acyl-CoA synthetase FadY had remarkable catalytic activity. Furthermore, the expression of fadY in transformed Xcc strain XC1 was investigated and shown to significantly attenuate bacterial pathogenicity on host plants, such as Chinese cabbage and radish. This is the first report demonstrating a DSF degradation enzyme from A. lactucae. Taken together, these findings shed light on the QQ mechanisms of A. lactucae strain QL-1, and provide useful enzymes and related genes for the biocontrol of infectious diseases caused by DSF-dependent bacterial pathogens.

scholarly journals Structural and Functional Characterization of Diffusible Signal Factor Family Quorum-Sensing Signals Produced by Members of the Burkholderia cepacia Complex

2010 ◽  
Vol 76 (14) ◽  
pp. 4675-4683 ◽  
Author(s):  
Yinyue Deng ◽  
Ji'en Wu ◽  
Leo Eberl ◽  
Lian-Hui Zhang
Keyword(s):  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
High Performance ◽  
Xanthomonas Campestris ◽  
Functional Characterization ◽  
Cell Communication ◽  
Burkholderia Cenocepacia ◽  
Burkholderia Cepacia Complex ◽  
Communication Signals ◽  
Diffusible Signal Factor

ABSTRACT Previous work has shown that Burkholderia cenocepacia produces the diffusible signal factor (DSF) family signal cis-2-dodecenoic acid (C12:Δ2, also known as BDSF), which is involved in the regulation of virulence. In this study, we determined whether C12:Δ2 production is conserved in other members of the Burkholderia cepacia complex (Bcc) by using a combination of high-performance liquid chromatography, mass spectrometry, and bioassays. Our results show that five Bcc species are capable of producing C12:Δ2 as a sole DSF family signal, while four species produce not only C12:Δ2 but also a new DSF family signal, which was identified as cis,cis-11-methyldodeca-2,5-dienoic acid (11-Me-C12:Δ2,5). In addition, we demonstrate that the quorum-sensing signal cis-11-methyl-2-dodecenoic acid (11-Me-C12:Δ2), which was originally identified in Xanthomonas campestris supernatants, is produced by Burkholderia multivorans. It is shown that, similar to 11-Me-C12:Δ2 and C12:Δ2, the newly identified molecule 11-Me-C12:Δ2,5 is a potent signal in the regulation of biofilm formation, the production of virulence factors, and the morphological transition of Candida albicans. These data provide evidence that DSF family molecules are highly conserved bacterial cell-cell communication signals that play key roles in the ecology of the organisms that produce them.

scholarly journals The roles of histidine kinases in sensing host plant and cell–cell communication signal in a phytopathogenic bacterium

2019 ◽  
Vol 374 (1767) ◽  
pp. 20180311 ◽  
Author(s):  
Fang-Fang Wang ◽  
Wei Qian
Keyword(s):  
Xanthomonas Campestris ◽  
Cell Communication ◽  
Research Progress ◽  
Bacterial Cells ◽  
Histidine Kinases ◽  
Phytopathogenic Bacterium ◽  
Bacterial Diseases ◽  
Signal Perception ◽  
Communication Signal ◽  
Diffusible Signal Factor

It has long been known that phytopathogenic bacteria react to plant-specific stimuli or environmental factors. However, how bacterial cells sense these environmental cues remains incompletely studied. Recently, three kinds of histidine kinases (HKs) were identified as receptors to perceive plant-associated or quorum-sensing signals. Among these kinases, HK VgrS detects iron depletion by binding to ferric iron via an ExxE motif, RpfC binds diffusible signal factor (DSF) by its N-terminal peptide and activates its autokinase activity through relaxation of autoinhibition, and PcrK specifically senses plant hormone–cytokinin and elicits bacterial responses to oxidative stress. These HKs are critical sensors that regulate the virulence of a Gram-negative bacterium, Xanthomonas campestris pv. campestris . Research progress on the signal perception of phytopathogenic bacterial HKs suggests that inter-kingdom signalling between host plants and pathogens controls pathogenesis and can be used as a potential molecular target to protect plants from bacterial diseases. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.

scholarly journals Characterization of a Novel N-Acylhomoserine Lactonase RmmL from Ruegeria mobilis YJ3

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 370 ◽  
Author(s):  
Xiulei Cai ◽  
Min Yu ◽  
Hu Shan ◽  
Xiaorong Tian ◽  
Yanfen Zheng ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
High Performance ◽  
Vibrio Anguillarum ◽  
Cell Communication ◽  
Quorum Quenching ◽  
Amino Acid Sequence Analysis ◽  
Chromatography Analysis ◽  
Potential Strategy

Gram-negative bacteria utilize N-acylhomoserine lactones (AHLs) as quorum sensing (QS) signaling molecules for intercellular communication. Cell-to-cell communication depends on cell population density, and AHL-dependent QS is related to the production of multiple genes including virulence factors. Quorum quenching (QQ), signal inactivation by enzymatic degradation, is a potential strategy for attenuating QS regulated bacterial infections. Both Gram-positive and -negative bacteria have QQ enzymes that can degrade AHLs. In our previous study, strain Ruegeria mobilis YJ3, isolated from healthy shrimp, showed strong AHLs degradative activity. In the current study, an AHL lactonase (designated RmmL) was cloned and characterized from Ruegeria mobilis YJ3. Amino acid sequence analysis showed that RmmL has a conserved “HXHXDH” motif and clusters together with lactonase AidC that belongs to the metallo-β-lactamase superfamily. Recombinant RmmL could degrade either short- or long-chain AHLs in vitro. High-performance liquid chromatography analysis indicated that RmmL works as an AHL lactonase catalyzing AHL ring-opening by hydrolyzing lactones. Furthermore, RmmL can reduce the production of pyocyanin by Pseudomonas aeruginosa PAO1, while for the violacein and the extracellular protease activities by Chromobacterium violaceum CV026 and Vibrio anguillarum VIB72, no significant reduction was observed. This study suggests that RmmL might be used as a therapeutic agent in aquaculture.

scholarly journals Killing Effect of Bacillus Velezensis FZB42 on a Xanthomonas Campestris pv. Campestris (Xcc) Strain Newly Isolated from Cabbage Brassica Oleracea Convar. Capitata (L.): A Metabolomic Study

2021 ◽  
Vol 9 (7) ◽  
pp. 1410
Author(s):  
Hynek Mácha ◽  
Helena Marešová ◽  
Tereza Juříková ◽  
Magdaléna Švecová ◽  
Oldřich Benada ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Inhibitory Effect ◽  
Side Chain ◽  
Bacillus Velezensis ◽  
Killing Effect ◽  
Close Observation ◽  
High Concentrations ◽  
Xanthomonas Campestris Pv Campestris ◽  
Potential Use ◽  
Metabolomic Study

The potential use of Bacillus velezensis FZB42 for biological control of various phytopathogens has been documented over the past few years, but its antagonistic interactions with xanthomonads has not been studied in detail. Novel aspects in this study consist of close observation of the death of Xanthomonas campestris pv. campestris cells in a co-culture with B. velezensis FZB42, and quantification of lipopeptides and a siderophore, bacillibactin, involved in the killing process. A new robust Xcc-SU isolate tolerating high concentrations of ferric ions was used. In a co-culture with the antagonist, the population of Xcc-SU was entirely destroyed within 24–48 h, depending on the number of antagonist cells used for inoculation. No inhibitory effect of Xcc-SU on B. velezensis was observed. Bacillibactin and lipopeptides (surfactin, fengycin, and bacillomycin) were present in the co-culture and the monoculture of B. velezensis. Except for bacillibactin, the maximum contents of lipopeptides were higher in the antagonist monoculture compared with the co-culture. Scanning electron microscopy showed that the death of Xcc-SU bacteria in co-culture was caused by cell lysis, leading to an enhanced occurrence of distorted cells and cell ghosts. Analysis by mass spectrometry showed four significant compounds, bacillibactin, surfactin, fengycin, and bacillomycin D amongst a total of 24 different forms detected in the co-culture supernatant: Different forms of surfactin and fengycin with variations in their side-chain length were also detected. These results demonstrate the ability of B. velezensis FZB42 to act as a potent antagonistic strain against Xcc.

scholarly journals Potential Use of some Petal Extracts against Xanthomonas campestris pv. campestris

Our Nature ◽  
1970 ◽  
Vol 9 (1) ◽  
pp. 100-106
Author(s):  
S.K. Bhardwaj ◽  
S.K. Singla ◽  
R.K. Bhardwaj
Keyword(s):  
Xanthomonas Campestris ◽  
Inhibitory Effect ◽  
Tagetes Erecta ◽  
Rosa Damascena ◽  
Causal Organism ◽  
Chrysanthemum Coronarium ◽  
Lathyrus Odoratus ◽  
Xanthomonas Campestris Pv Campestris ◽  
Potential Use ◽  
Anthocephalus Cadamba

The aqueous petal-extracts of 20 plants were screened by agar diffusion methods for their antibacterial activity against Xanthomonas campestris pv. campestris, a causal organism of black rot of cabbage and cauliflower. X. campestris pv. campestris was found most sensitive to the petal extracts of Tagetes erecta and Chrysanthemum coronarium. Some of the other plants such as Acacia fernesiana, Anthocephalus cadamba, Bombax malabaricum, Lathyrus odoratus, Rosa damascena and Thevetia nerifolia also showed the inhibitory effect against the test bacteria.DOI: http://dx.doi.org/10.3126/on.v9i1.5739

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