scholarly journals PAMAM-calix-dendrimers: Synthesis and Thiacalixarene Conformation Effect on DNA Binding

2021 ◽  
Vol 22 (21) ◽  
pp. 11901
Author(s):  
Olga Mostovaya ◽  
Pavel Padnya ◽  
Igor Shiabiev ◽  
Timur Mukhametzyanov ◽  
Ivan Stoikov
Keyword(s):  
Convenient Method ◽  
First Generation ◽  
Pamam Dendrimers ◽  
Cone Conformation ◽  
Partial Cone ◽  
Sperm Dna ◽  
Vis Spectroscopy ◽  
High Yields ◽  
First Time ◽  
Salmon Sperm Dna

A convenient method for the synthesis of the first generation PAMAM dendrimers based on the thiacalix[4]arene has been developed for the first time. Three new PAMAM-calix-dendrimers with the macrocyclic core in cone, partial cone, and 1,3-alternate conformations were obtained with high yields. The interaction of the obtained compounds with salmon sperm DNA resulted in the formation of the associates of the size up to 200 nm, as shown by the UV-Vis spectroscopy, DLS, and TEM. It was demonstrated by the CD method that the structure of the DNA did not undergo significant changes upon binding. The PAMAM-calix-dendrimer based on the macrocycle in cone conformation stabilized DNA and prevented its degradation.

scholarly journals Arylamine Analogs of Methylene Blue: Substituent Effect on Aggregation Behavior and DNA Binding

2021 ◽  
Vol 22 (11) ◽  
pp. 5847
Author(s):  
Alena Khadieva ◽  
Olga Mostovaya ◽  
Pavel Padnya ◽  
Valeriy Kalinin ◽  
Denis Grishaev ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Dna Binding ◽  
Dna Interaction ◽  
Sulfanilic Acid ◽  
New Drugs ◽  
Dimer Formation ◽  
Sperm Dna ◽  
Vis Spectroscopy ◽  
New Generation ◽  
First Time

The synthesis of new phenothiazine derivatives, analogs of Methylene Blue, is of particular interest in the design of new drugs, as well as in the development of a new generation of agents for photodynamic therapy. In this study, two new derivatives of phenothiazine, i.e., 3,7-bis(4-aminophenylamino)phenothiazin-5-ium chloride dihydrochloride (PTZ1) and 3,7-bis(4-sulfophenylamino)phenothiazin-5-ium chloride (PTZ2), are synthesized for the first time and characterized by NMR, IR spectroscopy, HRMS and elemental analysis. The interaction of the obtained compounds PTZ1 and PTZ2 with salmon sperm DNA is investigated. It is shown by UV-Vis spectroscopy and DFT calculations that substituents in arylamine fragments play a crucial role in dimer formation and interaction with DNA. In the case of PTZ1, two amine groups promote H-aggregate formation and DNA interactions through groove binding and intercalation. In the case of PTZ2, sulfanilic acid fragments prevent any dimer formation and DNA binding due to electrostatic repulsion. DNA interaction mechanisms are studied and confirmed by UV-vis and fluorescence spectroscopy in comparison with Methylene Blue. The obtained results open significant opportunities for the development of new drugs and photodynamic agents.

Synthesis of new porphyrin dimers linked by diamines and their supramolecular assemblies

2015 ◽  
Vol 19 (07) ◽  
pp. 874-886 ◽  
Author(s):  
Elena A. Mikhalitsyna ◽  
Vladimir S. Tyurin ◽  
Irina P. Beletskaya
Keyword(s):  
Supramolecular Assembly ◽  
Copper Cation ◽  
Supramolecular Assemblies ◽  
Amination Reaction ◽  
Vis Spectroscopy ◽  
Palladium Catalyzed ◽  
High Yields ◽  
First Time ◽  
Porphyrin Dimers

For the first time the bisporphyrins connected via diamine bridges were synthesized by palladium catalyzed Buchwald–Hartwig amination reaction with high yields. The diamine bridges include linear, cyclic and macrocyclic types. Homo- and heteronuclear metallocomplexes of bisporphyrins were investigated with UV-vis spectroscopy in the processes of supramolecular assembly. The interaction with diazabicyclo[2.2.2]octane (DABCO) and cesium and diammonium cations led to the coordination dimers. Control of the conformation of the bisporphyrin was realized via coordination of diamine moiety with copper cation.

scholarly journals Catechol-Containing Schiff Bases on Thiacalixarene: Synthesis, Copper (II) Recognition, and Formation of Organic-Inorganic Copper-Based Materials

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2334
Author(s):  
Pavel Padnya ◽  
Ksenia Shibaeva ◽  
Maxim Arsenyev ◽  
Svetlana Baryshnikova ◽  
Olga Terenteva ◽  
...  
Keyword(s):  
Nitrogen Atom ◽  
Ir Spectroscopy ◽  
Schiff Bases ◽  
Selective Recognition ◽  
Physical Methods ◽  
Imine Group ◽  
Partial Cone ◽  
Vis Spectroscopy ◽  
First Time ◽  
Thermal Stable

For the first time, a series of catechol-containing Schiff bases, tetrasubstituted at the lower rim thiacalix[4]arene derivatives in three stereoisomeric forms, cone, partial cone, and 1,3-alternate, were synthesized. The structure of the obtained compounds was proved by modern physical methods, such as NMR, IR spectroscopy, and HRMS. Selective recognition (Kb difference by three orders of magnitude) of copper (II) cation in the series of d-metal cations (Cu2+, Ni2+, Co2+, Zn2+) was shown by UV-vis spectroscopy. Copper (II) ions are coordinated at the nitrogen atom of the imine group and the nearest oxygen atom of the catechol fragment in the thiacalixarene derivatives. High thermal stable organic-inorganic copper-based materials were obtained on the base of 1,3-alternate + Cu (II) complexes.

Spectroscopic and electrochemical approaches for the Analysis of interaction between textile dye Reactive Red 231 and salmon sperm DNA

2021 ◽  
Author(s):  
Amira El-shobaky ◽  
Reda Elshafey ◽  
Abd-Elgawad Mohamed RADI
Keyword(s):  
Differential Pulse ◽  
Textile Dye ◽  
Artificial Agents ◽  
Visible Spectroscopy ◽  
Anodic Current ◽  
Sperm Dna ◽  
Vis Spectroscopy ◽  
Pulse Voltammetry ◽  
Dna Complex ◽  
Salmon Sperm Dna

DNA is one of the most critical targets of many artificial agents listed as carcinogens. Most of them irreversibly bind to the DNA inducing genome mutation; therefore, it is vital to study the nature of binding of these molecules to anticipate their toxicity. The interaction between textile dye reactive red 231 and salmon sperm double-strand DNA (ss-dsDNA) was investigated applying cyclic voltammetry (CV), differential pulse voltammetry (DPV) and ultraviolet-visible spectroscopy (UV/vis spectroscopy). The changes in the anodic current signals of dye were observed in the presence and absence of ss dsDNA at a glassy carbon electrode (GCE) using CV. The diffusion coefficient (D) was found to be 2.2× 10-7 and 9.5× 10-8 cm2s-1 from the CV data for the free dye and the dye-DNA complex, respectively. The electrochemical and UV/vis spectroscopy indicated a 1:1 complex formation of dye with DNA. The binding constant (kb) between the dye and DNA was calculated to be 5.4×105M-1 and 4.9×105M-1 in pH 4.0, using CV and UV/vis spectroscopy, respectively. The overall results suggest that dye binds to DNA through the combined effect of intercalation and electrostatic interaction. The damage of the DNA was also detected through the changes in the voltammetric behaviour of the dye.

An Expeditious One-Pot Three-Component Synthesis of 4-Aryl-3,4-dihydrobenzo[g] quinoline-2,5,10(1H)-triones under Green Conditions

2020 ◽  
Vol 23 (23) ◽  
pp. 2626-2634
Author(s):  
Saiedeh Kamalifar ◽  
Hamzeh Kiyani
Keyword(s):  
Reaction Medium ◽  
Meldrum's Acid ◽  
Meldrum’S Acid ◽  
One Pot ◽  
One Pot Synthesis ◽  
Substituted Benzaldehydes ◽  
High Yields ◽  
First Time ◽  
Green Conditions

: An efficient and facial one-pot synthesis of 4-aryl-3,4-dihydrobenzo[g]quinoline- 2,5,10(1H)-triones was developed for the first time. The process proceeded via the three-component cyclocondensation of 2-amino-1,4-naphthoquinone with Meldrum’s acid and substituted benzaldehydes under green conditions. The fused 3,4-dihydropyridin-2(1H)- one-ring naphthoquinones have been synthesized with good to high yields in refluxing ethanol as a green reaction medium. This protocol is simple and effective as well as does not involve the assistance of the catalyst, additive, or hazardous solvents.

QUINACRINE FLUORESCENCE AND Q-BANDING PATTERNS OF HUMAN CHROMOSOMES.: I. Effects of Varying Factors

1975 ◽  
Vol 17 (1) ◽  
pp. 81-92 ◽  
Author(s):  
C. C. Lin ◽  
H. van de Sande ◽  
W. K. Smink ◽  
D. R. Newton
Keyword(s):  
Exposure Time ◽  
Uv Light ◽  
Human Chromosomes ◽  
Concave Mirror ◽  
Banding Patterns ◽  
Fluorescent Microscope ◽  
Sperm Dna ◽  
Quinacrine Fluorescence ◽  
Well Differentiated ◽  
Salmon Sperm Dna

Various factors involved in the production of "Q-bands" have been studied. It was found that a Zeiss standard WL fluorescent microscope required a shorter exposure time for photography as compared to a Zeiss photomicroscope. The minimal exposure time was obtained when the standard WL microscope was equipped with a UV light source containing a DC powered mercury burner and a concave mirror. Further, the pH and type of water used in the staining, washing and mounting of the slide were also important factors in producing clear and well differentiated "Q-bands". It also appears that the factors involved in the production of "Q-bands" effect the enhancement or quenching of fluorescence by poly d(A-T).poly d(A-T) and salmon sperm DNA or poly dG∙poly dC respectively. This preliminary report also suggests that DNA or polynucleotides with a specific base sequence may play an important role in Q-banding patterns on chromosomes.

A CONVENIENT METHOD OF OBTAINING ASCOSPORES FROM BAKERS' YEAST

1949 ◽  
Vol 27c (4) ◽  
pp. 179-189 ◽  
Author(s):  
A. M. Adams
Keyword(s):  
Convenient Method ◽  
Sodium Acetate ◽  
Solid Medium ◽  
Genetic Research ◽  
Yeast Cells ◽  
Sporulation Medium ◽  
Direct Transfer ◽  
Yeast Cultures ◽  
High Yields ◽  
Solid Nutrient Medium

The superiority of methods involving the use of sporulation media containing acetate, first introduced by Stantial and Elder, over several commonly employed methods is established. A new method for obtaining ascospores from bakers' yeast cultures is recommended involving the direct transfer of vegetative cells from a solid nutrient medium to a solid medium containing acetate. High yields of ascospores are consistently produced after seven days' incubation. This method should lend itself particularly to use in the preparation of ascospores for instructional work, and for genetic research in yeast, and may also find application in yeast taxonomy. The technique recommended is as follows: vegetative yeast cells are multiplied on tomato juice agar or on dextrose nutrient agar, and are then transferred to a solid sporulation medium containing 0.04% dextrose, 0.14% anhydrous sodium acetate, and 2% agar.

Gold(I) Complexes Bearing P∩N-Ligands: An Unprecedented Twelve-membered Ring Structure Stabilized by Aurophilic Interactions

2009 ◽  
Vol 64 (11-12) ◽  
pp. 1513-1524 ◽  
Author(s):  
Uwe Monkowius ◽  
Manfred Zabel ◽  
Michel Fleck ◽  
Hartmut Yersin
Keyword(s):  
Solid State ◽  
Ring Structure ◽  
Phosphine Ligands ◽  
X Ray Crystallography ◽  
Aggregation Pattern ◽  
Elemental Analyses ◽  
High Yields ◽  
First Time ◽  
Solid State Structures ◽  
Aurophilic Interactions

The P∩N-ligands Ph2Pqn, 1, Ph2 Piqn, 2, Ph2 Ppym, 3, and the As∩N-ligands Ph2Asqn, 4, Ph2Asiqn, 5, (Ph = phenyl, qn = 8-quinoline, iqn = 1-isoquinoline, pym = 2-pyrimidine) have been synthesized, the ligands 2 and 5 for the first time. Their ligand properties were probed by the synthesis of gold(I) complexes. Reaction with (tht)AuCl (tht = tetrahydrothiophene) yielded the chlorogold complexes Ph2RP-Au-Cl (R = qn, 6; iqn, 7; pym, 8) and Ph2RAs-Au-Cl (R = qn, 9; iqn, 10) in high yields. Further treatment of 7 and 8 with one equivalent of AgBF4 provided the complexes [(Ph2Piqn)Au]BF4, 11, [(Ph2Ppym)Au]BF4, 12, and [(Ph2Piqn)Au(tht)]BF4, 14. For comparison, the previously reported complex [(Ph2Ppy)Au]BF4 (py = pyridine), 13, was re-investigated. The compounds were characterized by elemental analyses, mass spectrometry and NMR spectroscopy. In addition, the solid-state structures of 2, 3, 6, 7, 9 - 14 have been determined by X-ray crystallography. The chloro-gold compounds crystallize in the common rod-like structure known from R3EAuCl (R = aryl, E = P, As) complexes without further aggregation via aurophilic interactions. In all cases the phosphine acts as a monodentate ligand. In the solid state compounds 11 - 13 feature an unprecedented cyclic trinuclear aggregation pattern, in which the Au(I) atoms are linearly coordinated by the bridging phosphine ligands forming a cyclic (P-Au-N)3 arrangement. The resulting twelvemembered ring is further stabilized by Au · · · Au interactions. Due to the presence of these Au · · · Au contacts, 11 - 13 are emissive in the solid state but not in solution

scholarly journals Optimization of Green Synthesis of Silver Nanoparticles from Leaf Extracts ofPimenta dioica(Allspice)

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Akshay Rajeev Geetha ◽  
Elizabeth George ◽  
Akshay Srinivasan ◽  
Jameel Shaik
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Synthesis Method ◽  
Leaf Extracts ◽  
Synthesis Of Nanoparticles ◽  
Hot Air ◽  
Vis Spectroscopy ◽  
Pimenta Dioica ◽  
First Time

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.

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