scholarly journals Genetic Analysis of Hexaploid Wheat (Triticum aestivum L.) Using the Complete Sequencing of Chloroplast DNA and Haplotype Analysis of the Wknox1 Gene

2021 ◽  
Vol 22 (23) ◽  
pp. 12723
Author(s):  
Mari Gogniashvili ◽  
Yoshihiro Matsuoka ◽  
Tengiz Beridze

The aim of the presented study is a genetic characterization of the hexaploid wheat Triticum aestivum L. Two approaches were used for the genealogical study of hexaploid wheats—the complete sequencing of chloroplast DNA and PCR-based haplotype analysis of the fourth intron of Wknox1d and of the fifth-to-sixth-exon region of Wknox1b. The complete chloroplast DNA sequences of 13 hexaploid wheat samples were determined: Free-threshing—T. aestivum subsp. aestivum, one sample; T. aestivum subsp. compactum, two samples; T. aestivum subsp. sphaerococcum, one sample; T. aestivum subsp. carthlicoides, four samples. Hulled—T. aestivum subsp. spelta, three samples; T. aestivum subsp. vavilovii jakubz., two samples. The comparative analysis of complete cpDNA sequences of 20 hexaploid wheat samples (13 samples in this article plus 7 samples sequenced in this laboratory in 2018) was carried out. PCR-based haplotype analysis of the fourth intron of Wknox1d and of the fifth-to-sixth exon region of Wknox1b of all 20 hexaploid wheat samples was carried out. The 20 hexaploid wheat samples (13 samples in this article plus 7 samples in 2018) can be divided into two groups—T. aestivum subsp. spelta, three samples and T. aestivum subsp. vavilovii collected in Armenia, and the remaining 16 samples, including T. aestivum subsp. vavilovii collected in Europe (Sweden). If we take the cpDNA of Chinese Spring as a reference, 25 SNPs can be identified. Furthermore, 13–14 SNPs can be identified in T. aestivum subsp. spelta and subsp. vavilovii (Vav1). In the other samples up to 11 SNPs were detected. 22 SNPs are found in the intergenic regions, 2 found in introns, and 10 SNPs were found in the genes, of which seven are synonymous. PCR-based haplotype analysis of the fourth intron of Wknox1d and the fifth-to-sixth-exon region of Wknox1b provides an opportunity to make an assumption that hexaploid wheats T. aestivum subsp. macha var. palaeocolchicum and var. letshckumicum differ from other macha samples by the absence of a 42 bp insertion in the fourth intron of Wknox1d. One possible explanation for this observation would be that two Aegilops tauschii Coss. (A) and (B) participated in the formation of hexaploids through the D genome: Ae. tauschii (A)—macha (1–5, 7, 8, 10–12), and Ae. tauschii (B)—macha M6, M9, T. aestivum subsp. aestivum cv. ‘Chinese Spring’ and cv. ‘Red Doly’.

Genome ◽  
1993 ◽  
Vol 36 (5) ◽  
pp. 913-918 ◽  
Author(s):  
Michael E. Devey ◽  
Gary E. Hart

Hybridization of radiolabeled wheat DNA probes to genomic DNA digests of compensating nullisomic-tetrasomic lines and ditelosomic lines of hexaploid wheat (Triticum aestivum L. cv. Chinese Spring) can be used to identify intergenomic RFLPs. Sixty-three PstI/BamHI genomic DNA probes and eight cDNA probes were used to determine the chromosomal locations of 223 DNA fragments that define a minimum of 189 RFLP loci. Eighty-four percent of the genomic DNA clones hybridize to fragments located in homoeologous chromosomes and 16% hybridize to fragments located in one chromosome only or to fragments located in nonhomoeologous chromosomes. All of the cDNA probes hybridize to fragments located in homoeologous chromosomes.Key words: aneuploids, genetic mapping.


Genetics ◽  
1983 ◽  
Vol 103 (2) ◽  
pp. 313-321
Author(s):  
Paula R Neuman ◽  
J G Waines ◽  
K W Hilu ◽  
D Barnhart

ABSTRACT Two-dimensional paper chromatography was performed on methanol extracts of leaves of hexaploid bread wheat, Triticum aestivum L. em. Thell. cultivar Chinese Spring, and of the available nullisomic-tetrasomic compensating lines, the tetrasomic lines and the ditelocentric lines. The chromatograms had 27 spots identified as flavonoids and six representing phenolic acids. Some of the areas were complex and contained more than one compound. Four flavonoids were identified as under the control of gene(s) on chromosome arms 1DS, 4DL, 5AS and 6BS. A phenolic glycoside was concluded to be controlled by a gene(s) on chromosome arm 7BL. Gene(s) on chromosome arm 4DL affected the amount of compounds in two other spots, and gene(s) on chromosome arm 4BS reduced the level of all flavonoid compounds. The individual compounds in some of the complex spots may be under the control of gene(s) on homoeologous chromosomes.


2003 ◽  
Vol 31 (3-4) ◽  
pp. 331-338 ◽  
Author(s):  
S. Allouis ◽  
G. Moore ◽  
A. Bellec ◽  
R. Sharp ◽  
P. Faivre Rampant ◽  
...  

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 468-473 ◽  
Author(s):  
Ernest D. P. Whelan ◽  
G. B. Schaalje

Aneuploid seedlings of the common wheat (Triticum aestivum L.) cv. Chinese Spring (CS) that are nullisomic or telosomic for the long arm of chromosome 6D are susceptible to chilling injury under prolonged exposure to 6 °C; normal euploids or telosomics for the short arm are not. Studies of seedling grown for various durations at 20 °C prior to growth at 6 °C showed that chilling injury was a juvenile phenomenon and that the extent of injury was inversely proportional to the duration of growth at 20 °C to a maximum of about 14 days. When reciprocal crosses were made between susceptible 6D nullisomics or long-arm ditelocentrics of CS and resistant 6D nullisomics of three spring and one winter wheat cultivars, progenies from aneuploid F1 hybrids all segregated for susceptibility as a recessive trait and at a frequency approximating a dihybrid ratio; no cytoplasmic effects were detected. Aneuploids of the group 6 homoeologues of the spring wheat cvs. Cadet and Rescue were resistant, as were group 6 whole-chromosome substitutions of eight different donor wheats in the recipient parent CS and 56 other euploids tested. Genes for resistance to chilling injury appear to involve the group 6 chromosomes and the short arm of 6D in Chinese Spring. In contrast with chilling injury, all aneuploid lines with only four doses of the "corroded" loci on group 6 chromosomes exhibited chlorotic symptoms.Key words: Triticum aestivum, chilling injury.


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