Intermittent Hypoxic Therapy Inhibits Allogenic Bone-Graft Resorption by Inhibition of Osteoclastogenesis in a Mouse Model

Systemic Intermittent Hypoxic Therapy (IHT) relies on the adaptive response to hypoxic stress. We investigated allogenic bone-graft resorption in the lumbar spine in 48 mice. The mice were exposed to IHT for 1 week before surgery or 1 week after surgery and compared with controls after 1 and 4 weeks. Complete graft resorption was observed in 33–36% of the animals in the control group, but none in the preoperative IHT group. Increased bone-graft volume was demonstrated by micro-computed tomography in the preoperative IHT group after 1 week (p = 0.03) while a non-significant difference was observed after 4 weeks (p = 0.12). There were no significant differences in the postoperative IHT group. Increased concentration of immune cells was localized in the graft area, and more positive tartrate-resistant acid phosphatase (TRAP) staining was found in controls compared with IHT allogenic bone grafts. Systemic IHT resulted in a significant increase of the major osteoclast inhibitor osteoprotegerin as well as osteogenic and angiogenic regulators Tgfbr3, Fst3l, Wisp1, and Vegfd. Inflammatory cytokines and receptor activator of nuclear factor kappa-B ligand (RANKL) stimulators IL-6, IL-17a, IL-17f, and IL-23r increased after 1 and 4 weeks, and serum RANKL expression remained constant while Ccl3 and Ccl5 decreased. We conclude that the adaptive response to IHT activates numerous pathways leading to inhibition of osteoclastic activity and inhibition of allogenic bone-graft resorption.

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Replacement Process of Carbonate Apatite by Alveolar Bone in a Rat Extraction Socket

Materials ◽

10.3390/ma14164457 ◽

2021 ◽

Vol 14 (16) ◽

pp. 4457

Author(s):

Xiaoxu Zhang ◽

Ikiru Atsuta ◽

Ikue Narimatsu ◽

Nobuyuki Ueda ◽

Ryosuke Takahashi ◽

...

Keyword(s):

Bone Formation ◽

Alveolar Bone ◽

Autogenous Bone ◽

Control Group ◽

Tartrate Resistant Acid Phosphatase ◽

Carbonate Apatite ◽

Micro Computed Tomography ◽

Extraction Socket ◽

Bone Replacement ◽

Significant Difference

The objective of this study was to investigate a bone graft substitute containing carbonate apatite (CO3Ap) to analyze bone replacement and the state of bone formation in vitro and in vivo compared with autogenous bone (AB) or control. An osteoclast precursor cell line was cultured with AB or CO3Ap, and morphological analysis using scanning electron microscopy and a tartrate-resistant acid phosphatase activity assay were performed. The right maxillary first and second molars of Wistar rats were extracted and compensated by AB or CO3Ap granules. Following implantation, the bone formation state was evaluated after 3, 5, 7, 14, and 28 days of surgery by micro-computed tomography and immunohistostaining. The osteoclast-like cell morphology was typical with many cell protrusions in the AB and CO3Ap groups. Additionally, the number of osteoclast-like cells formed in the culture increased in each group; however, there was no significant difference between the AB and CO3Ap groups. Five days after tooth extraction, osteoclasts were observed near CO3Ap. The bone thickness in the CO3Ap group was significantly increased than that in the control group and the bone formation in the CO3Ap group increased by the same level as that in the AB group. CO3Ap is gradually absorbed by osteoclasts in the extraction socket and is easily replaced by alveolar bone. The process of bone replacement by osteoclasts is similar to that of autologous bone. By observing the process of bone replacement in more detail, it may be possible to gain a better understanding of the bone formation and control the amount of bone after surgery.

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Regenerative Therapy Modality for Treatment of True Combined Endodontic-Periodontal Lesions: A Randomized Controlled Clinical Trial

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18126220 ◽

2021 ◽

Vol 18 (12) ◽

pp. 6220

Author(s):

Reham AlJasser ◽

Sundus Bukhary ◽

Mohammed AlSarhan ◽

Dalal Alotaibi ◽

Saleh AlOraini ◽

...

Keyword(s):

Bone Graft ◽

Bone Grafting ◽

Endodontic Treatment ◽

Control Group ◽

Controlled Clinical Trial ◽

Bony Defect ◽

Keratinized Tissue ◽

Group I ◽

Significant Difference ◽

Grafting Procedure

The aim of this in-vivo study was to evaluate/compare the clinical periodontal parameters in patients with true combined endo-perio lesions (EPL), treated with gutta-percha (GP) and mineral trioxide (MTA) as an obturation material alone and with addition of bone grafting in such lesions. 120 Saudi patients (mean age = 41yrs) diagnosed with true combined EPL participated in this study. Group I (control group, n = 30) was treated with conventional endodontic treatment using GP for obturation. Group II (n = 30) was treated with conventional endodontic treatment using MTA for obturation. Group III (n = 30) was treated with conventional endodontic treatment using GP for obturation + grafting procedure to fill the bony defect. Group IV (n = 30) was treated with conventional endodontic treatment using MTA for obturation + grafting procedure to fill the bony defect. Clinical parameters (Pocket depth (PD); Clinical attachment loss (CAL); keratinized tissue width (KTW); gingival phenotype (G.Ph.) and Cone Beam Computed Tomography Periapical Index (CBCTPAI)) were recorded and compared at baseline, 3, 6, 12 months’ interval. For the groups III and IV, CBCTPAI showed significant difference (p < 0.0001) with the other groups at 6 months and 1-year interval. The group with MTA + bone graft showed 76% and 90% patients with 0 score at 6 months and 1-year follow-up, respectively. Comparison of mean values of PD among study groups at 3 months, 6 months and 1 year showed significant difference at 3 months, whereas the mean PD values of subjects in GP + bone graft showed significantly higher PD values than other 3 groups (p = 0.025). Use of GP and MTA for root canal obturation along with periodontal therapy and bone augmentation helps in resolving complex endo-perio lesions. Bone grafting in addition to obturation with MTA was found to be the best treatment strategy in management of EPL cases and is recommended for clinicians who are treating EPL patients.

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PTH1-34 improves devitalized allogenic bone graft healing in a murine femoral critical size defect

Injury ◽

10.1016/j.injury.2021.03.063 ◽

2021 ◽

Author(s):

Tito Rocha ◽

Amanda S. Cavalcanti ◽

Ana Carolina Leal ◽

Rhayra B. Dias ◽

Rafaela Sartore da Costa ◽

...

Keyword(s):

Bone Graft ◽

Critical Size ◽

Critical Size Defect ◽

Allogenic Bone ◽

Graft Healing ◽

Allogenic Bone Graft

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Fate of allogenic bone graft for sternal support in pectus excavatum repair

Asian Cardiovascular and Thoracic Annals ◽

10.1177/0218492315598423 ◽

2015 ◽

Vol 23 (8) ◽

pp. 961-965 ◽

Cited By ~ 1

Author(s):

Mahmoud Ashour ◽

Mohommad Rafay ◽

Waleed Saleh ◽

Mohamed Ahmed ◽

Hassan Robaidi ◽

...

Keyword(s):

Bone Graft ◽

Pectus Excavatum ◽

Allogenic Bone ◽

Allogenic Bone Graft

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Supercritical CO2 Viral-Inactivated Allogenic Bone Graft in Maxillary Sinus Augmentation Procedures: 10-Year Retrospective Clinical and Radiographic Results

The International Journal of Periodontics & Restorative Dentistry ◽

10.11607/prd.4877 ◽

2021 ◽

Vol 41 (3) ◽

pp. 433-441

Author(s):

Jean-Jacques Chalard

Keyword(s):

Bone Graft ◽

Maxillary Sinus ◽

Supercritical Co2 ◽

Sinus Augmentation ◽

Allogenic Bone ◽

Maxillary Sinus Augmentation ◽

Allogenic Bone Graft

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Histomorphometric Evaluation of Socket Preservation Using Autogenous Tooth Biomaterial and BM-MSC in Dogs

Scanning ◽

10.1155/2021/6676149 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Jin-Hyun Kim ◽

Puneet Wadhwa ◽

HongXin Cai ◽

Dong-Hyung Kim ◽

Bing Cheng Zhao ◽

...

Keyword(s):

Bone Graft ◽

Alveolar Bone ◽

Control Group ◽

Ridge Preservation ◽

Graft Material ◽

Socket Preservation ◽

Bone Graft Material ◽

Premolar Extraction ◽

Bone Width ◽

Significant Difference

This study is aimed at assessing the dimensional alterations occurring in the alveolar bone after premolar extraction in dogs with histomorphometric and histological analysis. After atraumatic premolar extraction, tooth-derived bone graft material was grafted in the extraction socket of the premolar region in the lower jaws of six dogs in two experimental groups. In the second experimental group, BM-MSCs were added together with the graft. The control was left untreated on the opposite side. After twelve weeks, all six animals were sacrificed. Differences in alveolar bone height crests lingually and buccally, and alveolar bone width at 1, 3, and 5 mm infracrestally, were examined. Histologic study revealed osteoconductive properties of tooth biomaterial. A statistically significant difference was detected between the test and control groups. In the test groups, a reduced loss of vertical and horizontal alveolar bone dimensions compared with the control group was observed. Tooth bone graft material may be considered useful for alveolar ridge preservation after tooth extraction, as it could limit the natural bone resorption process.

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The Short-term Follow-up Results of Open Wedge High Tibial Osteotomy with Using an Aescula Open Wedge Plate and an Allogenic Bone Graft: The Minimum 1-Year Follow-up Results

Clinics in Orthopedic Surgery ◽

10.4055/cios.2010.2.1.47 ◽

2010 ◽

Vol 2 (1) ◽

pp. 47 ◽

Cited By ~ 41

Author(s):

Su Chan Lee ◽

Kwang Am Jung ◽

Chang Hyun Nam ◽

Soong Hyun Jung ◽

Seung Hyun Hwang

Keyword(s):

Bone Graft ◽

High Tibial Osteotomy ◽

Tibial Osteotomy ◽

Open Wedge ◽

Short Term ◽

Allogenic Bone ◽

Wedge High Tibial Osteotomy ◽

Allogenic Bone Graft ◽

Wedge Plate

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Interaction of Anabolic Androgenic Steroids and Induced Tooth Movement in Rats

Brazilian Dental Journal ◽

10.1590/0103-6440201601119 ◽

2017 ◽

Vol 28 (4) ◽

pp. 504-510

Author(s):

Lilian Mary Karakida ◽

Cristiano Miranda de Araujo ◽

Aline Cristina Batista Rodrigues Johann ◽

Elisa Souza Camargo ◽

Orlando Motohiro Tanaka ◽

...

Keyword(s):

Blood Vessels ◽

Periodontal Ligament ◽

Tooth Movement ◽

Biological Effects ◽

Anabolic Androgenic Steroids ◽

Control Group ◽

Tartrate Resistant Acid Phosphatase ◽

Significant Difference ◽

Androgenic Steroids ◽

Orthodontic Devices

Abstract This study evaluated the interaction between tooth movement and two anabolic androgenic steroids (AAS), Deposteron® and Nebido®. One hundred Wistar rats were divided into 3 groups: control (C) n=30, Nebido experimental (N) n=35 and Deposteron experimental (D) n=35. The control group was subdivided into 6 subgroups: 1, 2, 3, 5, 7 and 14. The experimental groups were subdivided into 7 subgroups: 0, 1, 2, 3, 5, 7 and 14, which corresponded to the day of animal’s euthanasia after applying orthodontic force. Orthodontic devices were used to induce tooth movement using 50 cN of reciprocal force between the maxillary right first molar and the maxillary incisors. After euthanasia, the tissues were processed and stained with hematoxylin and eosin (HE) and tartrate-resistant acid phosphatase (TRAP). Osteoclasts, Howship’s lacunae and blood vessels were quantified. Groups N and D showed acceleration in the reorganization of the periodontal ligament compared to group C. The peak of the histological events occurred in group C on day 5 and in groups N and D on day 3 after installation of the orthodontic device. There was a statistically significant difference in the number of osteoclasts (p<0.05) between groups N3 and C3, and between groups N3 and D3. Supra-physiological doses of the AAS Nebido® and Deposteron® altered the number of osteoclasts, Howship’s lacunae and blood vessels, accelerating the reorganization of the periodontal ligament, resulting in accelerated biological effects from the induced tooth movement in rats.

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