scholarly journals Histomorphometric Evaluation of Socket Preservation Using Autogenous Tooth Biomaterial and BM-MSC in Dogs

Scanning ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Jin-Hyun Kim ◽  
Puneet Wadhwa ◽  
HongXin Cai ◽  
Dong-Hyung Kim ◽  
Bing Cheng Zhao ◽  
...  
Keyword(s):  
Bone Graft ◽  
Alveolar Bone ◽  
Control Group ◽  
Ridge Preservation ◽  
Graft Material ◽  
Socket Preservation ◽  
Bone Graft Material ◽  
Premolar Extraction ◽  
Bone Width ◽  
Significant Difference

This study is aimed at assessing the dimensional alterations occurring in the alveolar bone after premolar extraction in dogs with histomorphometric and histological analysis. After atraumatic premolar extraction, tooth-derived bone graft material was grafted in the extraction socket of the premolar region in the lower jaws of six dogs in two experimental groups. In the second experimental group, BM-MSCs were added together with the graft. The control was left untreated on the opposite side. After twelve weeks, all six animals were sacrificed. Differences in alveolar bone height crests lingually and buccally, and alveolar bone width at 1, 3, and 5 mm infracrestally, were examined. Histologic study revealed osteoconductive properties of tooth biomaterial. A statistically significant difference was detected between the test and control groups. In the test groups, a reduced loss of vertical and horizontal alveolar bone dimensions compared with the control group was observed. Tooth bone graft material may be considered useful for alveolar ridge preservation after tooth extraction, as it could limit the natural bone resorption process.

scholarly journals Socket preservation

2015 ◽  
Vol 27 (3) ◽  
Author(s):  
Caecilia Susetya Wahyu Nurhaeini ◽  
Ira Komara
Keyword(s):  
Connective Tissue ◽  
Bone Graft ◽  
Tooth Loss ◽  
Tooth Extraction ◽  
Graft Material ◽  
Alveolar Ridge ◽  
Socket Preservation ◽  
Bone Graft Material ◽  
Clinically Significant

Alveolar ridge will commonly decrease in volume and change morphologically, as a result of a tooth loss. These changes are usually clinically significant and can make placement of a conventional protesa  or an implant more difficult. Socket preservation after tooth extraction can minimize ridge resorption. By using socket preservation techniques, it is possible to preserve the height and width of the ridge. Socket preservation can be done by atraumatic tooth extraction, placement of bone graft material, membrane, combination of bone graft and membrane, and connective tissue graf.

scholarly journals Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

Materials ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 18 ◽  
Author(s):  
Jungwon Lee ◽  
Young-Jun Lim ◽  
Bongju Kim ◽  
Ki-Tae Koo ◽  
Yong-Moo Lee
Keyword(s):  
Bone Graft ◽  
Double Layer ◽  
Single Layer ◽  
Test Group ◽  
Control Group ◽  
Collagen Membrane ◽  
Ridge Preservation ◽  
Graft Material ◽  
Membrane Technique ◽  
Layer Membrane

The purpose of this study was to compare the histologic and radiologic differences between single- and double-layer collagen membrane techniques in flapless ridge preservation. The mandibular fourth premolar and first molar of four beagle dogs were used in the experiment. Mesial roots of the teeth were extracted and root canal treatment was performed at the distal roots. Ridge preservation was performed at the extraction sites using synthetic bone graft material. A single layer (control group) or double layer (test group) of non-crosslinked collagen membrane was applied following bone graft application. Three months later, the animals were sacrificed and micro-computed tomography (micro-CT) and histomorphometric analyses were conducted. Nonparametric Mann–Whitney test was performed to compare between the control and test groups. The vertical difference between buccal and lingual crests of control and test groups was 1.28 ± 0.41 and 0.53 ± 0.37 mm, respectively (p = 0.026). The mineralized bone area in control and test groups was 31.48% ± 7.41% and 42.25% ± 9.73%, respectively (p = 0.041). Within the limit of this study, ridge preservation using the double-layer membrane technique showed a reduced buccal bone resorption and improved new-bone formation in the ridge compared to that using the single-layer membrane technique.

scholarly journals Extraction socket preservation using a collagen plug combined withplatelet-rich plasma (PRP): A comparative clinico-radiographic study

2020 ◽  
Vol 14 (2) ◽  
pp. 139-145
Author(s):  
Numaan Nisar ◽  
Kumar Nilesh ◽  
Mushtaq Ishaq Parkar ◽  
Prashant Punde
Keyword(s):  
Wound Healing ◽  
Alveolar Bone ◽  
Root Anatomy ◽  
Extraction Socket ◽  
Socket Preservation ◽  
Bone Width ◽  
Alveolar Bone Remodeling ◽  
Significant Difference ◽  
Novel Material ◽  
The Difference

Background. Alveolar bone remodeling after tooth loss results in reduced ridge dimensions in horizontaland vertical planes. To prevent this, various authors have proposed different ridge preservationtechniques. A collagen plug is a novel material that has shown promising results in preserving thealveolar bone. PRP has also yielded favorable outcomes in wound healing and promoted osteoinductionand osteoconduction Methods. Thirty patients of both sexes with an age range of 30–18 years requiring bilateral extractionof teeth with similar tooth root anatomy in the maxilla or mandible were included in the study. Theextraction of teeth was carried out atraumatically. The patients’ arches were randomly divided andlabeled as the test or control sides. Bone width was measured on both sides. A collagen plug, with PRP,was placed, and the extraction socket was sutured on the test side. The control side was just sutured. Abaseline RVG was taken to record the apico-coronal height. The patients were recalled after 10 days forsuture removal and evaluation of wound healing. Parameters were re-evaluated at three and six monthspostoperatively. The data were subjected to t-test and one-way ANOVA. Results. The height of the crestal bone on the grafted side was more when compared to the non-graftedside three and six months after tooth extractions, and the difference was statically significant (P<0.001).No statistically significant difference was seen in the width of the alveolar bone three and six monthsafter tooth extraction (P>0.05). Conclusion. Collagen and PRP provided reasonable socket preservation as simple and inexpensiveoptions as compared to other materials.

scholarly journals TOXICOLOGICAL SCREENING OF ELLAGIC ACID IN POMEGRANATE FRUIT AND HYDROXYAPATITE COMBINATION AS BONE GRAFT MATERIAL ON BHK-21 FIBROBLAST CELL

2020 ◽  
Vol 5 (1) ◽  
pp. 98
Author(s):  
Agung Satria Wardhana ◽  
Isyana Erlita ◽  
Intan Nirwana ◽  
Hendrik Setiabudi
Keyword(s):  
Bone Graft ◽  
Ellagic Acid ◽  
Alternative Therapy ◽  
Fibroblast Cell ◽  
Control Group ◽  
Graft Material ◽  
Control Group Design ◽  
Bone Graft Material ◽  
Post Test

Background: Bone graft is an alternative therapy for periodontitis and other bone destructive lesions. Several studies had revealed Ellagic Acid (EA) ability in increasing osteogenesis process. EA contains polyphenols, such as Ellagitannin, Gallotannin, and Anthocyanin, which demonstrate anti-inflammatory and antibacterial activity as well as growth factor stimulating effect. EA combination with bone graft material (hydroxyapatite) is anticipated to enhance bone osteogenesis yet no investigation was performed to identify its toxicity towards fibroblast cell. Objective: To analyze EA toxicity on fibroblast cell in vitro. Methods: This was a true experimental study using post-test only with control group design. Fibroblast cell was exposed with EA in eight different concentrations: 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5%. Control group comprised of cell control and media group. All groups were exposed to MTT Assay test and measured using Elisa Reader. Result: The calculation of cell viability value in EA groups at 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5% concentration were 88.2%, 92.3%, 97.5%, 89.5%, 84.2%, 90.7%, 88.9% and 89.4% respectively. Conclusion: All EA and hydroxyapatite combinations are not toxic towards BHK-21 fibroblast cells.

scholarly journals Effects of hydroxyapatite gypsum puger scaffold applied to rat alveolar bone sockets on osteoclasts, osteoblasts and the trabecular bone area

2019 ◽  
Vol 52 (1) ◽  
pp. 13
Author(s):  
Amiyatun Naini ◽  
I Ketut Sudiana ◽  
Mohammad Rubianto ◽  
Utari Kresnoadi ◽  
Faurier Dzar Eljabbar Latief
Keyword(s):  
Bone Resorption ◽  
Trabecular Bone ◽  
Bone Tissue ◽  
Alveolar Bone ◽  
Control Group ◽  
Bone Area ◽  
Mandibular Incisor ◽  
Socket Preservation ◽  
Significant Difference ◽  
Trabecular Bone Area

Background: Damage to bone tissue resulting from tooth extraction will cause alveolar bone resorption. Therefore, a material for preserving alveolar sockets capable of maintaining bone is required. Hydroxyapatite Gypsum Puger (HAGP) is a bio-ceramic material that can be used as an alternative material for alveolar socket preservation. The porous and rough surface of HAGP renders it a good medium for osteoblast cells to penetrate and attach themselves to. In general, bone mass is regulated through a remodeling process consisting of two phases, namely; bone formation by osteoblasts and bone resorption by osteoclasts. Purpose: This research aims to identify the effects of HAGP scaffold application on the number of osteoblasts and osteoclasts, as well as on the width of trabecular bone area in the alveolar sockets of rats. Methods: This research used Posttest Only Control Group Design. There were three research groups, namely: a group with 2.5% HAGP scaffold, a group with 5% HAGP scaffold and a group with 10% HAGP scaffold. The number of samples in each group was six. HAGP scaffold at concentrations of 2.5%, 5% and 10% was then mixed with PEG (Polyethylene Glycol). The Wistar rats were anesthetized intra-muscularly with 100 mg/ml of ketamine and 20 mg/ml of xylazine base at a ratio of 1:1 with a dose of 0.08-0.2 ml/kgBB. Extraction of the left mandibular incisor was performed before 0.1 ml preservation of HAGP scaffold + PEG material was introduced into the extraction sockets and suturing was performed. 7 days after preparation of the rat bone tissue, an Hematoxilin Eosin staining process was conducted in order that observation under a microscope could be performed. Results: There were significant differences in both the number of osteoclasts and osteoblasts between the 2.5% HAGP group, the 5% HAGP group and the 10% HAGP group (p = 0.000). Similarly, significant differences in the width of the trabecular bone area existed between the 5% HAGP group and the 10% HAGP group, as well as between the 2.5% HAGP group and the 10% HAGP group (p=0.000). In contrast, there was no significant difference in the width of the trabecular bone area between the 2.5% HAGP group and the 5% HAGP group. Conclusion: The application of HAGP scaffold can reduce osteoclasts, increase osteoblasts and extend the trabecular area in the alveolar bone sockets of rats.

Accelerated Soft and Hard Tissue Healing In Extraction Sockets Augmented With Bone Allograft and Amnio- Fluid, Clinical and Histological Report of Ten Cases

2019 ◽  
Author(s):  
Mohamed Maksoud
Keyword(s):  
Bone Graft ◽  
Case Series ◽  
Bone Allograft ◽  
Gingival Tissue ◽  
Graft Material ◽  
Extraction Socket ◽  
Socket Preservation ◽  
Bone Graft Material ◽  
Keratinized Tissue ◽  
Bone Biopsies

Preservation of the dental extraction sockets through bone grafting has been a key procedure to maintain the volumetric aspect of the bone walls. This has been proven successful in bone preservation for future implant insertion or prosthetic treatment. Several materials of bone allograft, xenografts, synthetics and membranes were successful in socket preservation however the healing duration and the outcome of bone formed differs. Ten patients selected with posterior extraction sockets for the augmentation procedure, the bone graft mass mixed with Amnio-Spark fluid (Salvin Dental Specialties, Charlotte, NC, USA) and the wound secured with sutures and left to heal with no covering membrane. Post-operative visits scheduled at two, four and twelve weeks’ intervals for monitoring of the healing and core biopsies were harvested from the augmented extraction sockets and examined histologically at the twelve’s week. At two weeks, there was considerable formation of tissue covering the bone graft material which continued to progress into keratinized tissue in four weeks. Core bone biopsies taken at twelve weeks demonstrated a pattern of viable bone formation with minor remnants of the bone graft material. This case series will demonstrate the effectiveness of the amnio- fluid in rapid formation of gingival tissue on top of the augmented extraction socket wounds. Additionally, it will histologically confirm expedited bone remodeling into the extraction socket following the augmentation

Evaluation of Alveolar Bone Support of the Permanent Canine in Cleft and Noncleft Patients

2006 ◽  
Vol 43 (6) ◽  
pp. 678-682 ◽  
Author(s):  
Simon Boyarskiy ◽  
Hyung Jun Choi ◽  
Kitae Park
Keyword(s):  
Retrospective Study ◽  
Bone Graft ◽  
Alveolar Bone ◽  
Control Group ◽  
Significant Difference ◽  
History Of ◽  
Group A ◽  
Support Ratio ◽  
Bone Height ◽  
Average Bone

Objective: To quantitatively compare the alveolar bone support ratio of the permanent canine in cleft patients who received secondary alveolar bone graft with that of the population without clefts. Design: Retrospective study utilizing periapical radiographs of the subjects with and without clefts. Setting: Hospital and university based. Patients: Eighteen unilateral and 9 bilateral cleft patients who had secondary bone graft procedures. Main Outcome Measures: Alveolar bone support of the permanent canine utilizing the ratio of bone height to root length. Results: Average bone support for the permanent canine was 88.55% in patients with clefts and 95.59% in patients with no history of clefts. This difference was statistically significant. There was no statistically significant difference in alveolar bone support ratio between the unilateral and bilateral cleft patients. Conclusions: Although alveolar bone support was significantly higher in the noncleft control group, a successful level of alveolar bone support was achieved for the permanent canine on the cleft site after secondary bone graft. There was no difference in alveolar bone support achieved for the permanent canine whether the type of the cleft was unilateral or bilateral.

Guided Tissue Regeneration of the Mixture of Human Tooth-Ash and Plaster of Paris in Dogs

2007 ◽  
Vol 330-332 ◽  
pp. 1327-1330 ◽  
Author(s):  
Ha Ra Ku ◽  
Hyun Seon Jang ◽  
S.G. Kim ◽  
Moon Jin Jeong ◽  
Joo Cheol Park ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
Human Tooth ◽  
Control Group ◽  
Graft Material ◽  
Bone Graft Material ◽  
Plaster Of Paris ◽  
Experimental Group

This study evaluated the efficacy of periodontal tissue regeneration using guided tissue regeneration (GTR) with a mixture of tooth ash and plaster as a bone substitute material in the treatment of class II furcation defects in dogs. GTR was performed at the site of a surgically formed mandibular premolar bifurcation area in dogs. Four adult dogs were used in the animal study. The animals were in a good periodontal state without any systemic disease. A resorbable membrane (Bio-Gide®, Swiss) and bone graft material using a mixture of tooth ash and plaster were used to examine the biological activity. The defect in the control group was filled a Bio-Gide® only. For the experimental group, the defects were filled with a mixture of tooth ash and plaster and covered by a Bio-Gide®. Oral rinsing with 0.12% chlorhexidine was performed twice a day for 2 weeks. In control group, new bone formation was observed adjacent to the preexisting bone. Soft tissue ingrowth was also noted. In experimental group, new lamellar type trabecular bone formation as well as centum and PDL regeneration was observed in the base of the infrabony pocket. More bone regeneration would be expected when GTR is performed using a mixture of human tooth-ash and plaster of Paris as a bone graft material.

scholarly journals Loading and Release Profile Assay of Carbonated Hydroxyapatite Incorporated with Propolis as Bone Graft Material

2020 ◽  
Vol 25 (2) ◽  
pp. 121
Author(s):  
Indi Kusumawati ◽  
Suryono Suryono ◽  
Ahmad Syaify
Keyword(s):  
Bone Graft ◽  
Bacterial Adhesion ◽  
Alveolar Bone ◽  
Antibacterial Property ◽  
Graft Material ◽  
Synthetic Material ◽  
Carbonated Hydroxyapatite ◽  
Bone Graft Material ◽  
Absorbance Reading ◽  
The Difference

Periodontitis can lead to the destruction of the alveolar bone. The loss of the alveolar bone can be treated using carbonated hydroxyapatite (CHA) as a bone graft material. However, CHA is an alloplastic graft whose primary function is to act as a scaffold, but it is unable to stimulate the process of bone regeneration. Carbonated hydroxyapatite is an avascular synthetic material, which will increase the risk of bacterial adhesion on site that can lead to unsuccessful periodontal therapy. The incorporation of propolis into CHA is expected to add antibacterial capability into CHA. Besides its antibacterial property, propolis also has a bone regenerating effect. Mixing CHA with propolis needs to consider the process of loading the active ingredients into the carrier. The release of propolis is expected to occur gradually over a lengthy period. The purpose of this study was to analyze the loading and releasing assay for propolis incorporated with CHA. A propolis solution of 5%, 7.5%, and 10% was each incorporated into 10 mg of CHA. The loading percentage and releasing assay of propolis were measured. The absorbance reading was done at 289 nm using a UV-vis. It was shown that a 10% propolis solution had the highest loading percentage (32.08%), while the 5% propolis solution had the smallest loading percentage (10.63%). The propolis releasing profiles in all concentration groups were similar. The difference in propolis concentration incorporated with CHA affected the loading percentage but did not affect the propolis releasing assay.

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