scholarly journals First Record of the Entomopathogenic Nematode Steinernema litorale (Filipjev) (Rhabditida: Steinernematidae) and Its Symbiotic Bacterium from Turkey, and Its Efficacy Capability

Insects ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 144 ◽  
Author(s):  
Esengül Özdemir ◽  
Şerife Bayram ◽  
İ. Alper Susurluk
Keyword(s):  
Galleria Mellonella ◽  
Sandy Loam ◽  
Entomopathogenic Nematode ◽  
Symbiotic Bacterium ◽  
First Record ◽  
Soil Samples ◽  
Insect Larvae ◽  
Nature Park ◽  
First Time ◽  
Control Insect

The entomopathogenic nematode Steinernema litorale was isolated from Çamkoru Nature Park located in Ankara, Turkey, in September 2018. Steinernema litorale was recovered in 1 of 67 soil samples from a natural forest area; the soil was characterised as sandy loam. The isolated nematode S. litorale was identified based on morphological and molecular parameters. The symbiotic bacterium of S. litorale was determined as Xenorhabdus bovienii. Steinernema litorale was found for the first time in Turkey and the Middle East. The virulence of the isolate was tested on Galleria mellonella larvae. Different concentrations of the nematode (10, 25, 50, 75, and 100 infective juveniles (IJs/larvae) were used. While the LC50 values at 48 h, 72 h, and 96 h were 153.419, 51.005, and 15.439 IJs, respectively, and the LT50 values at 75 IJs and 100 IJs showed that this isolate is capable to control insect larvae within 50.083 and 36.266 h, respectively.

First record of female intersex in Hirschmanniella shamimi Ahmad, 1972 (Nematoda: Pratylenchidae), with a checklist of intersexes in plant nematodes

Zootaxa ◽  
2009 ◽  
Vol 1973 (1) ◽  
pp. 61-68 ◽  
Author(s):  
KAN ZHUO ◽  
JINLING LIAO ◽  
RUQIANG CUI ◽  
YUZHONG LI
Keyword(s):  
Guangdong Province ◽  
First Record ◽  
Reproductive Organs ◽  
Soil Samples ◽  
Rice Roots ◽  
First Time ◽  
Female Reproductive Organs

Two intersexes of Hirschmanniella shamimi are described and illustrated for the first time from soil samples around rice roots in Guangzhou, Guangdong Province, China. The two intersexes have developed female reproductive organs as well as rudimentary male sexual characters. A checklist of intersexes in plant nematodes is also given here.

scholarly journals Intraspecific variation among isolates of the entomopathogenic nematode Steinernema feltiae from Bull Island, Ireland

Nematology ◽  
2009 ◽  
Vol 11 (3) ◽  
pp. 439-451 ◽  
Author(s):  
Martin Downes ◽  
Conor Meade ◽  
Stephen Boyle ◽  
Alec Rolston ◽  
Thomae Kakouli-Duarte
Keyword(s):  
Intraspecific Variation ◽  
Galleria Mellonella ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Laboratory Culture ◽  
Steinernema Feltiae ◽  
Host Insect ◽  
Large Numbers ◽  
Insect Mortality ◽  
Control Insect

AbstractThe application of large numbers of entomopathogenic nematodes (EPN) to control insect pests of agriculture is likely to have an impact on the local EPN fauna, yet little is known about the intraspecific relationships between EPN populations, particularly with regard to phylogeny and outbreeding. Here we assess the fitness, with regards to fecundity, host insect mortality and time taken to produce progeny, of isolates of Steinernema feltiae from Bull Island, Ireland. Exon-primed, intron-crossing (EPIC) PCR was used to examine intraspecific phylogenies between S. feltiae isolates, and identified up to three possible colonisation events of Bull Island. EPIC-PCR grouped two isolates, 33.D.(2) and 59.F.(2), separately from the remaining ten S. feltiae isolates These same two isolates consistently performed poorly in all fitness assessments. Following the crossbreeding of all isolates in Galleria mellonella, the number of host cadavers exhibiting emerging infective juveniles was significantly fewer than expected and there were significant differences between isolates in the number of days until progeny were observed. Host insect mortality varied between 40 and 87%. Such intraspecific variation may be a result of adaptation to different microhabitats of Bull Island, which in turn may be accentuated by laboratory culture practices.

scholarly journals First report of the genus Heterorhabditis (Nematoda: Heterorhabditidae) from continental Portugal

2009 ◽  
Vol 46 (1) ◽  
pp. 45-48 ◽  
Author(s):  
V. Valadas ◽  
P. Vieira ◽  
S. Oliveira ◽  
M. Mota
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Dna ◽  
Galleria Mellonella ◽  
Entomopathogenic Nematode ◽  
Morphological Characterization ◽  
Soil Samples ◽  
First Report ◽  
Three Samples ◽  
Its Regions ◽  
Trapping Method

AbstractUntil recently, the only entomopathogenic nematode (EPN) species reported from continental Portugal, was of the genus Steinernema. Following a national survey of EPNs in continental Portugal, several natural and managed habitats have been surveyed in the southern part of the country. From 57 soil samples collected using the Galleria mellonella trapping method, three samples yielded EPN. Morphological characterization and sequence analysis of the ITS regions of ribosomal DNA allowed the identification of EPN isolates as Heterorhabditis bacteriophora, representing the first report of this genus for continental Portugal.

Mechanisms of specificity of association between the nematode Steinernema scapterisci and its symbiotic bacterium

Parasitology ◽  
1997 ◽  
Vol 114 (5) ◽  
pp. 483-488 ◽  
Author(s):  
P. S. GREWAL ◽  
M. MATSUURA ◽  
V. CONVERSE
Keyword(s):  
Galleria Mellonella ◽  
Entomopathogenic Nematode ◽  
Symbiotic Bacterium ◽  
Bacterial Symbiont ◽  
Steinernema Carpocapsae ◽  
Infective Juvenile ◽  
Symbiotic Bacteria ◽  
Bacterial Symbionts ◽  
First Report ◽  
Successful Isolation

We suggest a new mechanism for the maintenance of specificity of the association between the entomopathogenic nematode Steinernema scapterisci and its symbiotic bacteria. We evaluated the development and reproduction of infective and non-infective juvenile S. scapterisci in monoxenic combinations with its symbiotic bacteria, Xenorhabdus sp. ‘S’ and with the bacterial symbiont of Steinernema carpocapsae and Steinernema riobravis. Although development of non-infective stages occurred on all Xenorhabdus spp., the development of infective juveniles to the 4th stage (‘dauer’ recovery) was significantly delayed and reduced with X. nematophilus and Xenorhabdus sp. ‘R’, the bacterial symbionts of S. carpocapsae and S. riobravis, respectively. ‘Dauer’ recovery improved significantly when the cultures of X. nematophilus and Xenorhabdus sp. ‘R’ were supplemented with cell-free filtrates from Xenorhabdus sp. ‘S’. The infective juvenile S. scapterisci produced in all 3 cultures were virulent to Galleria mellonella larvae, confirming successful retention of Xenorhabdus from other steinernematids in their intestine. In fact, S. scapterisci infective juveniles containing X. nematophilus or Xenorhabdus sp. ‘R’ were more virulent to G. mellonella than those containing their natural symbiont, Xenorhabdus sp. ‘S’. We believe that this is the first demonstration of the symbiont-specific exit of infective juveniles from the ‘dauer’ phase which represents the finest level of specificity of bacteria–nematode association. This is also the first report of successful isolation of the natural symbiont of S. scapterisci.

scholarly journals A survey of entomopathogenic nematodes of the families Steinernematidae and Heterorhabditidae (Nematoda: Rhabditida) in the north-west of Iran

Nematology ◽  
2009 ◽  
Vol 11 (1) ◽  
pp. 107-116 ◽  
Author(s):  
Naser Eivazian Kary ◽  
Gholamreza Niknam ◽  
Seyed Abolgasem Mohammadi ◽  
Christine Griffin ◽  
Mohammad Moghaddam
Keyword(s):  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Heterorhabditis Bacteriophora ◽  
Common Species ◽  
Soil Samples ◽  
Steinernema Carpocapsae ◽  
Steinernema Feltiae ◽  
North West ◽  
The North ◽  
First Time

AbstractDuring 2002-2004, a survey of entomopathogenic nematodes was conducted for the first time in Iran throughout the three provinces in the north-west of the country. Soil samples were tested for the presence of steinernematid and heterorhabditid nematodes by baiting with Galleria mellonella larvae. Of the 833 soil samples studied 27 were positive for entomopathogenic nematodes (3.2%), with 17 (2.0%) containing Heterorhabditis and ten (1.2%) Steinernema isolates. Morphological and molecular studies were carried out to characterise isolates. The Heterorhabditis isolates were identified as Heterorhabditis bacteriophora and Steinernema as Steinernema carpocapsae, S. bicornutum and S. feltiae. Heterorhabditis bacteriophora was the most common species, which was isolated from 17 sites across the three provinces. Steinernema feltiae was the most common species of Steinernema, which was isolated from eight sites but in only two provinces. Steinernema carpocapsae and S. bicornutum were each isolated from only one site. Steinernema spp. were isolated mainly from orchards and grasslands but Heterorhabditis was isolated mainly from grasslands and alfalfa fields.

Photorhabdus luminescens subsp. noenieputensis subsp. nov., a symbiotic bacterium associated with a novel Heterorhabditis species related to Heterorhabditis indica

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1853-1858 ◽  
Author(s):  
Tiarin Ferreira ◽  
Carol van Reenen ◽  
Sylvie Pagès ◽  
Patrick Tailliez ◽  
Antoinette P. Malan ◽  
...  
Keyword(s):  
Type Species ◽  
Galleria Mellonella ◽  
Entomopathogenic Nematode ◽  
Symbiotic Bacterium ◽  
Tween 20 ◽  
Haemolytic Activity ◽  
Phenotypic Traits ◽  
Photorhabdus Luminescens ◽  
Content Type ◽  
Link Type

The bacterial symbiont AM7T, isolated from a novel entomopathogenic nematode species of the genus Heterorhabditis, displays the main phenotypic traits of the genus Photorhabdus and is highly pathogenic to Galleria mellonella. Phylogenetic analysis based on a multigene approach (16S rRNA, recA, gyrB, dnaN, gltX and infB) confirmed the classification of isolate AM7T within the species Photorhabdus luminescens and revealed its close relatedness to Photorhabdus luminescens subsp. caribbeanensis , P. luminescens subsp. akhurstii and P. luminescens subsp. hainanensis . The five concatenated protein-encoding sequences (4197 nt) of strain AM7T revealed 95.8, 95.4 and 94.9 % nucleotide identity to sequences of P. luminescens subsp. caribbeanensis HG29T, P. luminescens subsp. akhurstii FRG04T and P. luminescens subsp. hainanensis C8404T, respectively. These identity values are less than the threshold of 97 % proposed for classification within one of the existing subspecies of P. luminescens . Unlike other strains described for P. luminescens , strain AM7T produces acid from adonitol, sorbitol and xylitol, assimilates xylitol and has no lipase activity on medium containing Tween 20 or 60. Strain AM7T is differentiated from P. luminescens subsp. caribbeanensis by the assimilation of N-acetylglucosamine and the absence of haemolytic activity. Unlike P. luminescens subsp. akhurstii , strain AM7T does not assimilate mannitol, and it is distinguished from P. luminescens subsp. hainanensis by the assimilation of trehalose and citrate, the inability to produce indole from tryptophan and the presence of acetoin production and urease activity. Strain AM7T ( = ATCC BAA-2407T  = DSM 25462T) belongs to a novel subspecies, and is proposed as the type strain of Photorhabdus luminescens subsp. noenieputensis sp. nov.

Use of a monoclonal antibody in a field evaluation of the persistence and infectivity of Steinernema n. sp. D1 (Nematoda: Steinernematidae)

Nematology ◽  
2000 ◽  
Vol 2 (4) ◽  
pp. 425-434 ◽  
Author(s):  
Paul Richardson ◽  
Gary Keane ◽  
Steve Long ◽  
Deena Willmott
Keyword(s):  
Monoclonal Antibody ◽  
Galleria Mellonella ◽  
Entomopathogenic Nematode ◽  
Field Evaluation ◽  
Soil Samples ◽  
Bacterial Symbiont ◽  
Low Level ◽  
Anticorps Monoclonal ◽  
Phorid Flies ◽  
Introduced Population

AbstractAt the end of a mushroom cropping house trial designed to determine the infectivity, to phorid flies, of the entomopathogenic nematode Steinernema D1 (isolate IOW45), the spent, nematode-infested substrate was deposited as a stack on the edge of a field. The introduced population of Steinernema D1 (IOW45) was monitored by bi-monthly sampling for eight months. The infectivity, to Galleria mellonella larvae, of nematodes extracted from cores of substrate taken from the stack, was determined. There was a decline (P < 0.01) in the numbers of nematodes extracted on each of the sample dates. After the first sample was taken, fewer (P < 0.001) nematodes were recovered from the top layer of the stack than from the bottom or middle layers. Nematode infectivity declined (P < 0.001) over the first two months in the field and then remained at a constant low level until month 8, when no G. mellonella were parasitised. After removal of the stack, subsequent soil samples taken from plots that had been underlying, or adjacent to it, were baited with G. mellonella larvae. Samples of the haemolymph taken from larvae that died in the soil were tested with a monoclonal antibody specific to the bacterial symbiont (Xenorhabdus sp.) of Steinernema D1 (IOW45). Using this method, Steinernema D1 (IOW45) was detectable in soil that had been underlying the compost stack for as long as 12 months after compost deposition. Utilisation d'anticorps monoclonaux pour l'évaluation au champ de la persistanceet du pouvoir infestant de Steinernema n. sp. D1 (Nematoda: Steinernematidae) - A la fin d'un essai sur une culture de champignons sous abri, essai destiné à déterminer le pouvoir infestant du nématode entomopathogène Steinernema D1 (isolat IOW45) envers des Diptères Phoridae, le substrat utilisé, infesté de nématodes, a été rassemblé en une meule, au coin du champ. La population de Steinernema D1 (IOW45) a ensuite été évaluée par des prélèvements bimensuels pendant 8 mois. Le pouvoir infestant des nématodes envers des larves de Galleria mellonella a été déterminé en prélevant des carottes dans la meule de substrat. Il s'est révélé que le nombre de nématodes extraits diminue (P < 0,01) au cours des prélèvements successifs. Après le premier prélèvement, il est récupéré moins de nématodes (P < 0,001) au sommet de la meule qu'au milieu ou au bas de celle-ci. Au champ, le pouvoir infestant du nématode décroit (P < 0,001) pendant les 2 premiers mois et reste ensuite au même niveau jusqu'au huitième mois où aucun G. mellonella n'est plus parasité. Après enlèvement de la meule, des échantillons supplémentaires dans le sol situé sous la meule ou à proximité de celle-ci ont été pourvus d'appâts (larves de G. mellonella). Des échantillons d'hémolymphe provenant de larves mortes dans le sol ont été testés à l'aide d'un anticorps monoclonal spécifique de la bactérie symbiote (Xenorhabdus sp.) de Steinernema D1 (IOW45). En utilisant cette méthode, Steinernema D1 (IOW45) peut être détecté dans le sol situé sous la meule pendant une période d'au moins 2 mois après l'enlèvement du compost.

scholarly journals First record of Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) in Slovenia

2009 ◽  
Vol 46 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Ž. Laznik ◽  
T. Tóth ◽  
T. Lakatos ◽  
M. Vidrih ◽  
S. Trdan
Keyword(s):  
Laboratory Experiments ◽  
Entomopathogenic Nematodes ◽  
Plant Protection ◽  
Entomopathogenic Nematode ◽  
First Record ◽  
Soil Samples ◽  
Steinernema Feltiae ◽  
Molecular Technique ◽  
Practical Application ◽  
Recorded Instance

AbstractIn October 2007 we examined 80 soil samples from 16 different locations in the central part of Slovenia (the Notranjska region) and confirmed the presence of entomopathogenic nematodes only in two soil samples. This represents the first recorded instance of an entomopathogenic nematode in Slovenia. In sample B30 we confirmed the presence of Steinernema feltiae (Rhabditida: Steinernematidae) by means of a molecular technique. In Slovenia the application of entomopathogenic nematodes was hitherto possible only in laboratory experiments, while the Rules on Biological Plant Protection made the practical application of exotic organisms in the domestic environment entirely impossible. After the first record of the entomopathogenic nematode S. feltiae we expect the aformentioned agent to become an important alternative to insecticides in plant protection against pest insects.

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