scholarly journals The Influence of Temperature and Host Gender on Bacterial Communities in the Asian Citrus Psyllid

Insects ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1054
Author(s):  
Rui-Xu Jiang ◽  
Feng Shang ◽  
Hong-Bo Jiang ◽  
Wei Dou ◽  
Tomislav Cernava ◽  
...  
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
High Throughput ◽  
Bacterial Communities ◽  
High Temperature Treatment ◽  
Asian Citrus Psyllid ◽  
Abiotic Factor ◽  
Insect Physiology ◽  
And Gender ◽  
Low Temperature Treatments

The Asian citrus psyllid, D. citri Kuwayama is the primary vector for Candidatus Liberibacter asiaticus (CLas), which causes a destructive disease in citrus plants. Bacterial symbionts are important determinants of insect physiology, and they can be impacted by many external factors. Temperature is an important abiotic factor affecting insect physiology, and it is also known that differences in symbiont proportions may vary in different insect genders. To date, it is unclear how the symbionts of D. citri are affected by temperature and gender. This study used high-throughput sequencing of 16S ribosomal RNA amplicons to determine how temperature and gender affect the bacterial communities present in D. citri. We identified 27 amplicon sequence variants (ASVs) belonging to 10 orders, seven classes, and five phyla. The dominant phylum was Proteobacteria (99.93%). Other phyla, including Firmicutes, Bacteroidota, Deinococcota, Cyanobacteria, and Actinobacteriota, were less abundant (<0.1%). Profftella (71.77–81.59%) and Wolbachia (18.39–28.22%) were the predominant taxa in all samples. Under high-temperature treatment, Profftella was more common in females, while Wolbachia had a higher abundance in males. In males, Profftella was more abundant under low-temperature treatments than under high-temperature treatments. In contrast, Wolbachia showed a higher abundance under high-temperature treatments than under low-temperature treatments. An RT-qPCR (quantitative real-time PCR) approach confirmed the results obtained with high-throughput DNA sequencing. Our results provide a basis for understanding the co-adaptation of D. citri and its symbionts to environmental temperature stress.

Endometrial Thermal Balloon Ablation Using a High Temperature, Pulsed System: A Mathematical Model

2003 ◽  
Vol 125 (6) ◽  
pp. 841-851 ◽  
Author(s):  
Daniel M. Reinders ◽  
Susan A. Baldwin ◽  
Joel L. Bert
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Treatment Time ◽  
Uterine Cavity ◽  
Temperature Treatment ◽  
High Temperature Treatment ◽  
Low Temperature Treatment ◽  
Balloon Ablation ◽  
Pulsed Treatment ◽  
Thermal Balloon

A new endometrial thermal balloon ablation treatment for menorrhagia is modeled mathematically to predict its efficacy and safety. A device preheats a fluid to 173°C within a reservoir external to the uterus, and then pulses this fluid without further heating between the reservoir and the balloon for 2.1 min of treatment time. The model predicted this treatment to result in consistent immediate tissue death (coagulation) depths of 3.4±0.1 mm for uterine cavities of 7 to 26 mL, and that eventual necrosis (tissue death that would occur 1–5 days post burn) may occur to depths of 6.5±0.2 mm. Whereas, burn depths varied with uterine cavity volume when a low temperature treatment (constant 75°C for 15 min) was modeled (2.3–2.9 mm and 6.8–8.2 mm, for immediate tissue death and eventual necrosis respectively). Similarly, the high temperature, pulsed treatment was less sensitive to blood perfusion rate than the low temperature treatment. Predicted eventual necrosis depth was 1.5 mm less for the high temperature, pulsed treatment than that predicted for a low temperature treatment (constant 87°C for 7 min) for the same immediate tissue death depth (3.5 mm), indicating that the new high temperature treatment may result in less damage to non targeted tissues.

Respiration and mass loss rates of aspen and pine leaf litter decomposing in laboratory microcosms

1988 ◽  
Vol 66 (10) ◽  
pp. 1948-1959 ◽  
Author(s):  
Barry R. Taylor ◽  
Dennis Parkinson
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Mass Loss ◽  
Linear Function ◽  
Leaf Litter ◽  
Populus Tremuloides ◽  
Moisture Level ◽  
Respiration Rates ◽  
Mass Losses ◽  
Low Temperature Treatments

Respiration rates and mass losses of decomposing pine (Pinus contorta Loud. × P. banksiana Lamb.) and aspen (Populus tremuloides Michx.) leaf litter were compared in laboratory microcosms for a range of temperature and moisture levels. For both litter types, a pair of high-temperature treatments (18, 26 °C) and a pair of low-temperature treatments (2, 10 °C) were distinguishable on the basis of respiration rate, mass loss, shape of the respiration curve, and (for pine) estimated microbial efficiency. Respiration rates in high-temperature treatments showed an initial increase to a wide peak (wider and later at 18 than at 26 °C), followed by a sharp decline; respiration of low-temperature treatments was nearly constant through time or declined slowly. Moisture level (15, 30, or 60 mL∙week−1 watering rate) was less important than temperature in determination of mass losses or respiration rates. Aspen respiration at 18 and 26 °C peaked sooner and declined more rapidly at higher moisture levels than at lower ones; at 2 and 10 °C, higher moisture levels inhibited respiration owing to saturation. Mass loss of pine needles after 153 days decay was a linear function of temperature (R2 = 0.92). The best regression describing mass loss of aspen litter after 130 days decay was a linear function of both temperature and moisture, without interaction (R2 = 0.82). Moisture level became more influential as temperature increased. Researchers are cautioned about the limitations of cumulative respiration curves, and alternatives, such as ANOVA, correlation, and the runs test, are suggested.

scholarly journals Temperature-induced Glucosinolate Accumulation Is Associated with Expression of BrMYB Transcription Factors

HortScience ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Veronica L. Justen ◽  
Vincent A. Fritz
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Gene Expression Analysis ◽  
Tissue Type ◽  
Temperature Dependent ◽  
Temperature Regimes ◽  
Health Promoting ◽  
Transcription Factor Expression ◽  
Low Temperature Treatments

Turnips (Brassica rapa. subsp. rapa L.) produce glucosinolates (GSLs), thioglucosides whose hydrolyzed derivatives have been shown to provide chemopreventive benefits. Two cultivars of turnips [‘Just Right’ (JR) and ‘Scarlet Queen’ (SQ)] were grown under three different temperature regimes to assess the role of temperature on GSL production in roots and shoots. When compared with low-temperature treatments, high-temperature treatments increased total and individual GSLs in a tissue- and genotype-specific manner. When compared with low-temperature treatments, total GSLs were ≈70% and 130% higher in JR shoots and roots, respectively, grown at high-temperature treatments. High temperatures also increased total GSLs in SQ shoots and roots by ≈80% and 85%, respectively, when compared with low temperatures. Gluconasturtiin (GNS, 2-phenylethyl GSL) concentration was inversely correlated with temperature with high-temperature treatments resulting in 20% and 48% less GNS than low-temperature treatments in JR and SQ roots, respectively. The indolic GSL, 1-methoxyglucobrassicin (1MGB; 1-methoxy-3-ylmethyl GSL), was the root GSL most elevated by increased temperature resulting in a 1000% increase on average in both cultivars between the low- and high-temperature treatments. These results show promise for the use of temperature to enhance the health-promoting properties of turnip because 1MGB has potent chemopreventive effects. Gene expression analysis suggests that some BrMYB transcription factor expression levels are associated with temperature-dependent changes in GSL accumulation; however, this association varies between cultivar and tissue type.

Exsolution and inversion in pigeonite from the Whin Sill, Northern England

1978 ◽  
Vol 36 (1) ◽  
pp. 474-475
Author(s):  
P.P.K. Smith
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Homogeneous Nucleation ◽  
Silicate Mineral ◽  
Antiphase Boundaries ◽  
Two Phase ◽  
Primitive Form ◽  
The Core ◽  
Nucleation Mechanisms ◽  
And Inversion

Grains of pigeonite, a calcium-poor silicate mineral of the pyroxene group, from the Whin Sill dolerite have been ion-thinned and examined by TEM. The pigeonite is strongly zoned chemically from the composition Wo8En64FS28 in the core to Wo13En34FS53 at the rim. Two phase transformations have occurred during the cooling of this pigeonite:- exsolution of augite, a more calcic pyroxene, and inversion of the pigeonite from the high- temperature C face-centred form to the low-temperature primitive form, with the formation of antiphase boundaries (APB's). Different sequences of these exsolution and inversion reactions, together with different nucleation mechanisms of the augite, have created three distinct microstructures depending on the position in the grain.In the core of the grains small platelets of augite about 0.02μm thick have farmed parallel to the (001) plane (Fig. 1). These are thought to have exsolved by homogeneous nucleation. Subsequently the inversion of the pigeonite has led to the creation of APB's.

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