scholarly journals Calprotectin and Receptor for Advanced Glycation End Products as a Potential Biomarker in Abdominal Aortic Aneurysm

2020 ◽  
Vol 9 (4) ◽  
pp. 927
Author(s):  
Willy Hauzer ◽  
Wojciech Witkiewicz ◽  
Jan Gnus
Keyword(s):  
Abdominal Aortic Aneurysm ◽  
Aortic Aneurysm ◽  
Advanced Glycation End Products ◽  
Endovascular Aneurysm Repair ◽  
Control Group ◽  
Advanced Glycation ◽  
Abdominal Aortic ◽  
Potential Biomarker ◽  
Glycation End Products ◽  
End Products

Experiments conducted in recent years on animals and research works worldwide show a linkage between calprotectin and occurrence and development of the abdominal aortic aneurysm (AAA). Additionally, a correlation between the level of the receptor for advanced glycation end products (RAGE) and the diameter of the abdominal aorta was found. The purpose of this study was to investigate whether calprotectin and the RAGE plasma level may be a biomarker of human AAA occurrence. We determined two groups of research participants: a group of 32 patients aged 53–88 undergoing primary endovascular aneurysm repair and a control group of 43 volunteers aged 59–82 without the AAA. All the patients from the study group had their blood samples drawn in order to determine the level of calprotectin and RAGE in plasma. The second follow-up examination was carried out after three months. The concentration of calprotectin and RAGE in plasma was determined with the use of the immunoenzymatic method (ELISA). The study showed that patients with the AAA had significantly higher mean calprotectin and RAGE plasma levels (p = 0.0001 and p = 0.0002, respectively) as compared to the control group. After the AAA repair operations, the level of concentration of the calprotectin decreased significantly (p = 0.0002). So far, no studies on the connection between the increase of the calprotectin and RAGE in the patient’s plasma with the AAA have been published. Calprotectin may be a promising biomarker related to the occurrence of AAA. Larger studies are needed to fully elucidate and confirm the role of calprotectin in the development and progression of the aneurysm.

scholarly journals Skin accumulation of advanced glycation end products is increased in patients with an abdominal aortic aneurysm

2017 ◽  
Vol 66 (6) ◽  
pp. 1696-1703.e1 ◽  
Author(s):  
Jeltje Boersema ◽  
Lisanne C. de Vos ◽  
Thera P. Links ◽  
Douwe J. Mulder ◽  
Andries J. Smit ◽  
...  
Keyword(s):  
Abdominal Aortic Aneurysm ◽  
Aortic Aneurysm ◽  
Advanced Glycation End Products ◽  
Advanced Glycation ◽  
Abdominal Aortic ◽  
Skin Accumulation ◽  
Glycation End Products ◽  
End Products

scholarly journals MiR-92b-3p is Induced by Advanced Glycation End Products and Involved in the Pathogenesis of Diabetic Nephropathy

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Li-ping Wang ◽  
Jia-nan Geng ◽  
Bo Sun ◽  
Cheng-bo Sun ◽  
Yan Shi ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Mirna Expression ◽  
Expression Profiles ◽  
Rat Kidney ◽  
Luciferase Reporter ◽  
Control Group ◽  
Age Group ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products

Purpose. The current study aims to examine the effects of advanced glycation end products (AGEs) on the microRNA (miRNA) expression profile in the kidney tissues of rats. Methods. Wistar rats were randomly divided into three equal experiment groups: the AGE group, the RSA group, and the control group. The rats in the AGE group and the RSA group were administered with advanced glycation end products (AGEs) and rat serum albumin (RSA) via the tail vein, respectively, whereas the control group received PBS. Total RNA was prepared from the rat kidney tissues, and the miRNA expression profiles in different experiment groups were compared by microarray analysis. The expression levels of selected differential miRNAs were verified by RT-qPCR. Target gene prediction was conducted using algorithms such as TargetScan, miRanda, and PICTar. Functional analysis was performed to determine the putative biological roles of the validated miRNAs. Results. The microarray study revealed 451 upregulated and 320 downregulated miRNAs in the AGE group compared with the RSA group (p<0.05). Seven miRNAs, including miR-21-5p, miR-92b-3p, miR-140-3p, miR-196a-5p, miR-181b-5p, miR-186-5p, and miR-192-5p, were screened and verified using RT-qPCR, of which, the change of miR-92b-3p was the most obvious according to the miRNA expression different multiple and p value. Furthermore, the expression trend of miR-92b-3p measured by RT-qPCR was shown to be consistent with the microarray results. Bioinformatics analysis and luciferase reporter assay identified Smad7 was a direct target of miR-92b-3p. Both immunohistochemical and western blotting showed that Smad7 expression was significantly suppressed in the kidney tissues from the AGE group compared with the control and RSA groups. Conclusion. The results of the current study suggested that miR-92b-3p could mediate AGE-induced development of renal abnormalities through targeting Smad7 in rats with DN.

scholarly journals Soluble Receptor for Advanced Glycation End Product: A Biomarker for Acute Coronary Syndrome

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Louise J. N. Jensen ◽  
Allan Flyvbjerg ◽  
Mette Bjerre
Keyword(s):  
Acute Coronary Syndrome ◽  
Advanced Glycation End Products ◽  
Acute Ischemia ◽  
Soluble Receptor ◽  
Advanced Glycation ◽  
Coronary Syndrome ◽  
Potential Biomarker ◽  
Glycation End Products ◽  
End Products ◽  
Artery Disease

The receptor of advanced glycation end products (RAGE) and its ligands are linked to the pathogenesis of coronary artery disease (CAD), and circulating soluble receptor of advanced glycation end products (sRAGE), reflecting the RAGE activity, is suggested as a potential biomarker. Elevated sRAGE levels are reported in relation to acute ischemia and this review focuses on the role of sRAGE as a biomarker for the acute coronary syndrome (ACS). The current studies demonstrated that sRAGE levels are elevated in relation to ACS, however during a very narrow time period, indicating that the time of sampling needs attention. Interestingly, activation of RAGE may influence the pathogenesis and reflection in sRAGE levels in acute and stable CAD differently.

scholarly journals Cardioprotective effect of Volvariella volvacea in streptozotocin administered rats

2016 ◽  
Vol 11 (4) ◽  
pp. 903 ◽  
Author(s):  
Soosaimanickam Carmel Punitha ◽  
Muthu Rajasekaran
Keyword(s):  
Advanced Glycation End Products ◽  
Glycosylated Hemoglobin ◽  
Lipid Peroxides ◽  
Protein Carbonyls ◽  
Control Group ◽  
Volvariella Volvacea ◽  
Advanced Glycation ◽  
Group I ◽  
Glycation End Products ◽  
End Products

<p class="Abstract">The present study examined the cardioprotective role of methanol extract of the edible mushroom <em>Volvariella volvacea</em> against oxidative stress in hyperglycemic rats. Rats divided into 6 groups were administered with nicotinamide and streptozotocin intraperitoneally, except Group I (control). Group II served as diabetic control. Group III was given glibenclamide. Two groups (IV and V) of rats received (200 and 400 mg/kg) mushroom extracts orally for 30 days and Group VI received vitamin E (40 mg/kg). After the treatment period, lipid peroxides, carbonyl end products, advanced glycation end products, reduced glutathione, glutathione peroxidase, glutathione-S transferase, catalase, superoxide dismutase and non-enzymatic anti-oxidants (vitamin C and E) were assessed in the heart tissues of experimental animals. Glycosylated hemoglobin was estimated in blood. Electrocardiography recordings of the treated groups were also done. The results showed that mushroom extract treatment reduced the lipid peroxides, advanced glycation end products and protein carbonyls significantly and reversed the altered anti-oxidant enzymes, and the vitamins.</p><p class="Abstract"><strong>Video Clips</strong>:</p><p><a href="https://www.youtube.com/v/wn01m31-XhY">Hemolysate preparation</a>: 2 min 50 sec</p><p><a href="https://www.youtube.com/v/B0OLbU-NdEM">Mixing, glycosylated hemoglobin separation and reading absorbance</a>: 3 min 10 sec</p><p> </p>

scholarly journals The Association of Serum Thrombomodulin with Endothelial Injuring Factors in Abdominal Aortic Aneurysm

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Magdalena Budzyń ◽  
Bogna Gryszczyńska ◽  
Wacław Majewski ◽  
Zbigniew Krasiński ◽  
Magdalena Paulina Kasprzak ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Abdominal Aortic Aneurysm ◽  
Endothelial Damage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Advanced Glycation ◽  
Abdominal Aortic ◽  
Glycation End Products ◽  
End Products ◽  
And Oxidative Stress ◽  
Age And Sex

Background.The aim of the present study was to evaluate the concentration of serum thrombomodulin (sTM) in the AAA patients and to examine its correlation with various factors which may potentially participate in the endothelial injury.Materials and Methods.Forty-one patients with AAA were involved and divided into subgroups based on different criteria. Concentration of sTM was measured using enzyme-linked-immunosorbent assay (ELISA). The results were compared with those obtained in 30 healthy age- and sex-matched volunteers.Results.The higher concentration of sTM was observed in AAA patients compared with those in controls volunteers [2.37 (1.97–2.82) ng/mL versus 3.93 (2.43–9.20) ng/mL,P< 0.001]. An elevated sTM associated significantly with increased triglycerides (TAG) [P= 0.022], cholesterol [P= 0.029], hsCRP [P= 0.031], and advanced glycation end products (AGEs) [P= 0.033].Conclusions.The elevation of serum sTM level suggests that endothelial damage occurs in AAA pathogenesis. The correlations observed indicate that lipids abnormalities, inflammation, and oxidative stress may be involved in this destructive process.

scholarly journals Role of Advanced Glycation End Products in Assessment of Diabetes Mellitus as a Risk Factor for Retinal Vein Occlusion

2021 ◽  
Vol 11 (17) ◽  
pp. 7934
Author(s):  
Karolina Kaźmierczak ◽  
Paweł Żuchowski ◽  
Katarzyna Łapińska-Duczmal ◽  
Katarzyna Zabel ◽  
Zofia Sikorska ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Retinal Vein Occlusion ◽  
Advanced Glycation End Products ◽  
Systemic Hypertension ◽  
Control Group ◽  
Skin Autofluorescence ◽  
Advanced Glycation ◽  
Vein Occlusion ◽  
Glycation End Products ◽  
End Products

Aim: In this study, we aimed to assess the correlation between diabetes mellitus (DM) and the retinal vein occlusion (RVO) based on skin autofluorescence (SAF) measurement, which reflects the accumulation of advanced glycation end products (AGE) in patients who have undergone an episode of central retinal vein occlusion (CRVO) or branch retinal vein occlusion (BRVO). Material and methods: In total, 23 patients (16 males, 7 females) with RVO were included in this study. Among these 23 participants, 12 (52%) had been diagnosed with CRVO and 11 (48%) with BRVO. The control group consisted of 14 healthy volunteers (11 females, 3 males). To calculate the risk of cardiovascular diseases (CVD) and DM, we conducted SAF examinations. We compared the SAF levels in three groups of patients: (1) with CRVO, (2) with BRVO, and (3) the control group. Basic demographic and clinical information and detailed history of the concurrent diagnoses of systemic diseases, such as systemic hypertension (HTN), DM, hyperlipidemia (HL), and heart diseases, were obtained. Results: In total, 10 (43.5%) patients were diagnosed with DM, 6 (55%) in the BRVO group and 4 (33%) in the CRVO group. The mean SAF value was significantly higher in the BRVO group than in the control group (2.64 a.u. and 2.35 a.u., respectively) (p = 0.023). More patients with risk of DM were identified in the CRVO group than in the BRVO group (p = 0.024). Conclusions: The advanced glycation end products (AGE) in the skin autofluorescence (SAF) is a viable method of evaluating the risk of DM in patients with RVO. We confirmed a correlation between RVO and DM, which was significantly pronounced in the CRVO form, although further carefully devised studies on the relationship between RVO and DM with a larger number of responders should be conducted in the future.

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