scholarly journals The Influence of Gestational Diabetes Mellitus upon the Selected Parameters of the Maternal and Fetal System of Insulin-Like Growth Factors (IGF-1, IGF-2, IGFBP1-3)—A Review and a Clinical Study

2020 ◽  
Vol 9 (10) ◽  
pp. 3256
Author(s):  
Tomasz Gęca ◽  
Anna Kwaśniewska
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Peripheral Blood ◽  
Control Group ◽  
Study Group ◽  
Igfbp 3 ◽  
Insulin Like Growth Factors

Background: Gestational diabetes mellitus (GDM), defined as impaired glucose tolerance with onset or first recognition in pregnancy, increases the risk of not only maternal but also fetal and neonatal complications. Given the structural similarity of insulin-like growth factors with insulin and participation of components of the insulin-like growth factor system in glucose homeostasis, we hypothesized that the IGF axis is involved in the development of GDM complications or its pathogenesis. The aim of this study was to evaluate the effect of GDM on the selected parameters of the insulin-like growth factors (IGF-1, IGF-2, IGFBP1-3) in the maternal and fetal blood. Methods: The clinical material of this case-control study included 109 pregnant women and their offspring. The study group (n = 120) consisted of 60 patients with diagnosed gestational diabetes and their newborn babies. The control group (n = 98) comprised 49 healthy parturients and their offspring. We measured the concentrations of IGF-1, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, insulin and glucose made by the ELISA method in peripheral blood serum in patients suffering from GDM and pregnant women without GDM, and in the umbilical cord blood of newborn babies born to them. Results: The analysis of concentrations of IGF-1, -2 and IGFBP-3 in peripheral blood as well as umbilical cord blood did not demonstrate a statistically significant difference between the study group and the control group. Significantly lower concentration of IGFBP-1, IGFBP-2 in peripheral blood and in umbilical cord blood was detected in the study group in comparison to the control group. A statistically positive correlation between the concentration of IGF-1 in umbilical cord serum of newborn babies born to women with gestational diabetes and the length of a baby after its birth was observed. Conclusions: Gestational diabetes mellitus does not significantly affect the concentrations of IGF-1, -2, IGFBP-3 in the peripheral blood and umbilical cord blood, but has the greatest influence on maternal and fetal IGFBP-2 concentrations. A positive correlation between the concentration of IGF-1 in umbilical cord blood and the length of a newborn suggests an influence of IGF-1 on the process of fetal development.

scholarly journals Gestational Diabetes Mellitus in Pregnancy Increased Erythropoietin Level Affecting Differentiation Potency of Haematopoietic Stem Cell of Umbilical Cord Blood

2021 ◽  
Vol 8 ◽  
Author(s):  
Mohd Razif Mohd Idris ◽  
Fazlina Nordin ◽  
Zaleha Abdullah Mahdy ◽  
S. Fadilah Abd Wahid
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cell ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
In Utero ◽  
Haematopoietic Stem Cell ◽  
Control Group

Background: The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and haematopoietic stem cell (HSC) quality. The aim of this study is to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC in terms of differentiation potency including the UCB parameters used for banking and transplantation purposes.Methods: UCB-HSC was collected from 42 GDM and 38 normal pregnancies. UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme linked immunoassay (ELISA) technique.Result: The UCB parameters; volume, total nucleated count (TNC) and total CD34+ cells were significantly reduced in the GDM group compared to the control group. The number of HSC progenitors' colonies were significantly reduced in the GDM group except for progenitor BFU-E, which was significantly increased (GDM = 94.19 ± 6.21, Control = 73.61 ± 2.73, p = 0.010). This data was associated with higher EPO level in GDM group. However, the insulin level in the GDM group was comparable to the Control group.Conclusion: Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy such as GDM might affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

scholarly journals Gestational diabetes mellitus in pregnancy increased erythropoietin level affecting differentiation potency of haematopoietic stem cell of umbilical cord blood

2020 ◽  
Author(s):  
Mohd Razif Mohd Idris ◽  
Fazlina Nordin ◽  
Zaleha Abdullah Mahdy ◽  
S Fadilah Abd Wahid
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cell ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
In Utero ◽  
Haematopoietic Stem Cell ◽  
Control Group

Abstract Background The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines, and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and quality of haematopoietic stem cell (HSC). This study aims to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC regarding its differentiation potency, including the UCB parameters used for banking and transplantation purposes. Methods UCB-HSC was collected from 42 GDM and 38 normal pregnancies. UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme-linked immunoassay (ELISA) technique. Results The UCB parameters, i.e., volume, total nucleated count (TNC), and total CD34+ cells, were significantly reduced in the GDM group compared to the control group. The number of HSC progenitor colonies was significantly reduced in the GDM group, except for progenitor BFU-E, which was significantly increased (GDM = 94.19 ± 6.21, Control = 73.61 ± 2.73, p = 0.010). This data was associated with a higher EPO level in the GDM group. However, the insulin level in the GDM group was comparable to the control group. Conclusion Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy, such as GDM might affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

scholarly journals Gestational diabetes mellitus in pregnancy increased erythropoietin level affecting differentiation potency of haematopoietic stem cell of umbilical cord blood

2020 ◽  
Author(s):  
Fazlina Nordin ◽  
Mohd Razif Mohd Idris ◽  
Zaleha Abdullah Mahdy ◽  
S Fadilah Abd Wahid
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cell ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Haematopoietic Stem Cell ◽  
Control Group ◽  
In Utero Environment

Abstract Background The in utero environment has many factors that can support cell differentiation. Cytokines, chemokines and growth factors play big roles in haematopoietic mechanisms. Some diseases like gestational diabetes mellitus (GDM) might affect the environment and haematopoietic stem cell (HSC) quality. The aim of this study is to investigate the adverse effects of GDM on umbilical cord blood (UCB) HSC in terms of differentiation potency including the UCB parameters used for banking and transplantation purposes. Methods UCB-HSC was isolated and further enriched using immuno-magnetic separation beads (MACS). The UCB-HSC were cultured in methylcellulose media to investigate the differentiation potency. The level of erythropoietin (EPO) and insulin in the UCB plasma was measured using enzyme linked immunoassay (ELISA) technique. Results The UCB parameters; volume, total nucleated count (TNC) and total CD34+ cells were significantly reduced in the GDM group compared to the control group. The number of HSC progenitors’ colonies were significantly reduced in the GDM group except for progenitor BFU-E, which was significantly increased (GDM=94.19 ± 6.21, Control=73.61 ± 2.73, p=0.010 ). This data was associated with higher EPO level in GDM group. However, the insulin level in the GDM group was comparable to the Control group. Conclusion Our results suggest that the changes in the in utero environment due to abnormalities during pregnancy such as GDM affect the differentiation potency of UCB-HSC. These findings can be considered as an additional parameter for the inclusion and exclusion criteria for UCB banking, particularly for mothers with GDM.

scholarly journals Mid-late pregnancy maternal glycolipids in gestational diabetes mellitus (GDM) fluctuated in glycosylated hemoglobin levels of 5.5%-6.4% associated with umbilical cord blood of amino acid and carnitine profiles: a nested case-cohort observational study

2020 ◽  
Author(s):  
long yuhang ◽  
Le Chen ◽  
Yaochao Yang ◽  
Wei Liu ◽  
Hanxi Zhang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Amino Acid ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Glycosylated Hemoglobin ◽  
Control Group ◽  
Diabetic Medicine

Abstract Background To explore the relationship between maternal-neonatal clinical data and the changes of cord blood amino acid and carnitine levels between pregnant women with gestational diabetes mellitus (GDM) when glycosylated hemoglobin levels of 5.5%-6.4% excluded diabetic medicine as GDMa group and the control group. Methods In all, 312 qualified participants were recruited with permission from the ethical department of the Fifth Affiliated Hospital of Sun Yat-Sen University and divided into two groups (GDMa and non-GDM) after adjusting for maternal age and body mass index (BMI) between the 1 June 2017 and 30 April 2020. All maternal-neonatal data were collected and analysed at the centre. Results Interestingly, glycine in cord blood was not only significantly different between groups(594.19 vs 407.45, P < 0.001), but also associated among those pregnant women in GDM when glycosylated hemoglobin levels of 5.5%-6.4% through the standard of diet control and exercise (b = 0.449, P = 0.010). Meanwhile, neonatal hypoglycemia had the positive association with GDM monitoring glycosylated hemoglobin levels of 5.5%-6.4% (P = 0.031, OR 5.77 95%CI[1.18, 28.36]), but none of correlations with umbilical cord blood of amino acids and carnitines. Conclusions The study identifies some differences and relationships in maternal-neonatal data only when GDMa group, glycosylated hemoglobin levels of 5.5%-6.4% without diabetic medicine, compared with the control group which adjusted by age and BMI. Particularly, umbilical cord blood of glycine levels is related to the diabetic status of glycosylated hemoglobin 5.5%-6.4% in GDM during pregnancy and could be potential diabetic mechanism by maternal-fetal interface.

Maternal, placental and cordonal metallomic profiles in gestational diabetes mellitus

Metallomics ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 676-685 ◽  
Author(s):  
Marco Roverso ◽  
Valerio Di Marco ◽  
Denis Badocco ◽  
Paolo Pastore ◽  
Marilia Calanducci ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Diabetic Mothers

The concentration of several elements changes in umbilical cord blood of fetuses from diabetic mothers and from controls.

scholarly journals Circular RNA expression profiles in umbilical cord blood exosomes from normal and gestational diabetes mellitus patients

2020 ◽  
Vol 40 (11) ◽  
Author(s):  
Minkai Cao ◽  
Le Zhang ◽  
Yu Lin ◽  
Zhengying Li ◽  
Jianjuan Xu ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Expression Profiles ◽  
Circular Rna ◽  
Cellular Functions ◽  
Pathway Analyses

Abstract Circular RNA (circRNA) is a novel member of endogenous noncoding RNAs with widespread distribution and diverse cellular functions. Recently, circRNAs have been identified for their enrichment and stability in exosomes. However, the roles of circRNAs from umbilical cord blood exosomes in gestational diabetes mellitus (GDM) occurrence and fetus growth remains poorly understood. In the present study, we used microarray technology to construct a comparative circRNA profiling of umbilical cord blood exosomes between GDM patients and controls. We found the exosome particle size was larger, and the exosome concentration was higher in the GDM patients. A total of 88,371 circRNAs in umbilical cord blood exosomes from two groups were evaluated. Of these, 229 circRNAs were significantly up-regulated and 278 circRNAs were significantly down-regulated in the GDM patients. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses demonstrated that circRNA parental genes involved in the regulation of metabolic process, growth and development were significantly enriched, which are important in GDM development and fetus growth. Further circRNA/miRNA interactions analysis showed that most of the exosomal circRNAs harbored miRNA binding sites, and some miRNAs were associated with GDM. Collectively, these results lay a foundation for extensive studies on the role of exosomal circRNAs in GDM development and fetus growth.

Maternal and umbilical cord blood subfatin and spexin levels in patients with gestational diabetes mellitus

Peptides ◽  
2020 ◽  
Vol 126 ◽  
pp. 170277
Author(s):  
Seyda Yavuzkir ◽  
Kader Ugur ◽  
Rulin Deniz ◽  
Dondu Ulker Ustebay ◽  
Miyase Mirzaoglu ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood
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