scholarly journals Antibody CD133 Biofunctionalization of Ammonium Acryloyldimethyltaurate and Vinylpyrrolidone Co-Polymer-Based Coating of the Vascular Implants

Materials ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 5634
Author(s):  
Przemysław Sareło ◽  
Maciej Duda ◽  
Marlena Gąsior-Głogowska ◽  
Edyta Wysokińska ◽  
Wojciech Kałas ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Protein Molecule ◽  
Attenuated Total Reflection ◽  
In Vitro Studies ◽  
Covalent Attachment ◽  
Thiol Groups ◽  
Vascular Stents ◽  
Free Thiol ◽  
Fourier Transform Raman Spectroscopy

Current vascular stents, such as drug eluting stents (DES), have some serious drawbacks, like in stent restenosis and thrombosis. Therefore, other solutions are sought to overcome these post-implantations complications. These include the strategy of biofunctionalization of the stent surface with antibodies that facilitate adhesion of endothelial cells (ECs) or endothelial progenitor cells (EPCs). Rapid re-endothelialization of the surface minimizes the risk of possible complications. In this study, we proposed ammonium acryloyldimethyltaurate/vinylpyrrolidone co-polymer-based surface (AVC), which was mercaptosilanized in order to expose free thiol groups. The presence of free thiol groups allowed for the covalent attachment of CD133 antibodies by disulfide bridges formation between mercaptosilanized surface and cysteine of the protein molecule thiol groups. Various examinations were performed in order to validate the procedure, including attenuated total reflection–Fourier transform infrared spectroscopy (ATR-FTIR) and Fourier transform Raman spectroscopy (FT-Raman), atomic force microscopy (AFM) and scanning electron microscopy (SEM). By means of ATR-FTIR spectroscopy presence of the CD133 antibody within coating was confirmed. In vitro studies proved good biocompatibility for blood cells without induction of hemolytic response. Thus, proposed biofunctionalized CD133 antibody AVC surface has shown sufficient stability for adapting as cardiovascular implant coating and biocompatibility. According to conducted in vitro studies, the modified surface can be further tested for applications in various biological systems.

In vitro andin vivo identification of ?pseudocatalase? activity in Dead Sea water using Fourier transform Raman spectroscopy

2002 ◽  
Vol 33 (7) ◽  
pp. 586-592 ◽  
Author(s):  
Karin U. Schallreuter ◽  
Jeremy Moore ◽  
Stefanie Behrens-Williams ◽  
Angela Panske ◽  
Marco Harari ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fourier Transform ◽  
Sea Water ◽  
Dead Sea ◽  
Fourier Transform Raman Spectroscopy ◽  
Fourier Transform Raman

scholarly journals Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer

2013 ◽  
Vol 49 (2) ◽  
pp. 285-292 ◽  
Author(s):  
Mangesh Bhalekar ◽  
Savita Sonawane ◽  
Shamkant Shimpi
Keyword(s):  
Rheological Properties ◽  
Covalent Attachment ◽  
Synthesis And Characterization ◽  
Thiol Groups ◽  
Chemically Modified ◽  
Mucoadhesive Polymer ◽  
Disulphide Bonds ◽  
Group Content

The aim of this study was to improve the mucoadhesive potential of xyloglucan polymer by the covalent attachment of cysteine as thiol moiety. The parent polymer xyloglucan was chemically modified by introducing sulphydryl bearing compound L-cysteine HCl. Different batches of xyloglucan-cysteine conjugates were prepared at varying reaction pH (2-6) and evaluated for optimum thiol incorporation, disulphide group content, swelling behavior, rheological properties and mucoadhesive properties. The obtained conjugates characterized in vitro by quantification of immobilized thiol groups; showed maximum thiol incorporation on xyloglucan (7.67 ± 0.14 %) at pH 5. The disulphide group content was found maximum (2.83 ± 0.12) at pH 6. The water uptake at end of 4 h was 5.0 for xyloglucan and was found to decrease in thiolated derivatives with increase in thiolation. Mucoadhesion studies revealed that mucoadhesion of xyloglucan-cysteine conjugate increased more than twice compared to the unmodified polymer. The viscosity of thiomer was more than that of xyloglucan because of formation of disulphide bonds.

Improvement of mechanical properties of hydroxyapatite composites reinforced with i-Al64Cu23Fe13 quasicrystal

2020 ◽  
pp. 002199832096455
Author(s):  
José Castañeda-Vía ◽  
Carlos V Landauro ◽  
Justiniano Quispe-Marcatoma ◽  
Ana Champi ◽  
Fred Montalvo ◽  
...  
Keyword(s):  
Fourier Transform ◽  
High Efficiency ◽  
Sol Gel ◽  
In Vitro Cytotoxicity ◽  
Mechanical Resistance ◽  
Compression Tests ◽  
Test Pieces ◽  
Fourier Transform Raman Spectroscopy ◽  
The Matrix

Mechanical behavior of hydroxyapatite-based composites (HAp) was studied as a function of the reinforcement concentration of the quasicrystalline (QC) Al64Cu23Fe13 alloy. The synthesis of the HAp matrix was carried out by sol-gel method, while the synthesis of the QC was performed by an arc furnace with a subsequent thermal treatment. The composites were made by powder metallurgy and cold compacted to form test pieces that were sintered with a constant flow of argon. The materials were characterized using X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and Fourier transform Raman spectroscopy. The study of mechanical strength was carried through compression tests. The biocompatibility of the composites was tested using an in-vitro cytotoxicity assay. The mechanical resistance of HAp/QC composites increased with the concentration of quasicrystalline reinforcement. Young’s modulus and compressive strength increased in 43% and 21%, respectively, with a 10 wt% QC reinforcement, which demonstrates an hybrid behaviour of the composite due to the inclusion of reinforcing particles in the pores of the matrix. This composite did not show cytotoxicity at any of the QC concentrations. A fabrication route is proposed as a fast, easy and high efficiency alternative for applications in the biomedical industry because of its high scalability potential.

In Vitro Bioactivity Study on ZnI2-HAp Nanoparticles

2020 ◽  
Vol 15 (4) ◽  
pp. 523-531
Author(s):  
Mohammad Y. Alshahrani ◽  
H. Algarni ◽  
Ibrahim AlShahrani ◽  
I. S. Yahia ◽  
H. Y. Zahran ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Sol Gel ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
Mouse Splenocytes ◽  
Fourier Transform Raman Spectroscopy ◽  
Sol Gel Process ◽  
Splenic Cells ◽  
Ft Ir

The manufactured powder of immaculate HAp which is doped with 1.0, 5.0, 10, 20, 30, and 40 in wt.% ZnI2 has been prepared via the sol–gel process. The characterization of samples was achieved by X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FT-IR) and Fourier Transform Raman spectroscopy (FT- Raman). The morphology of the obtained HAp powder was studied through SEM analysis. The investigation proves that all prepared samples have the dimension of a nanoscale. The prepared powders were examined for its anti-growth, stimulatory/cytotoxic potentials on normal/activated mouse splenocytes in vitro as well as the lytic effects on red blood cells (RBCs). Results showed that different preparations of ZnI2 had anti-growth effects on normal mouse splenocytes. The pure HAp, 1% and 10% ZnI2 showed no effects on stimulated mouse splenic cells while the other preparations 5%, 20%, 30% and 40% ZnI2 showed inhibitory/cytotoxic effects. All ZnI2 preparations showed safe effects on RBCs.

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