Improvement of mechanical properties of hydroxyapatite composites reinforced with i-Al64Cu23Fe13 quasicrystal

2020 ◽  
pp. 002199832096455
Author(s):  
José Castañeda-Vía ◽  
Carlos V Landauro ◽  
Justiniano Quispe-Marcatoma ◽  
Ana Champi ◽  
Fred Montalvo ◽  
...  
Keyword(s):  
Fourier Transform ◽  
High Efficiency ◽  
Sol Gel ◽  
In Vitro Cytotoxicity ◽  
Mechanical Resistance ◽  
Compression Tests ◽  
Test Pieces ◽  
Fourier Transform Raman Spectroscopy ◽  
The Matrix

Mechanical behavior of hydroxyapatite-based composites (HAp) was studied as a function of the reinforcement concentration of the quasicrystalline (QC) Al64Cu23Fe13 alloy. The synthesis of the HAp matrix was carried out by sol-gel method, while the synthesis of the QC was performed by an arc furnace with a subsequent thermal treatment. The composites were made by powder metallurgy and cold compacted to form test pieces that were sintered with a constant flow of argon. The materials were characterized using X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and Fourier transform Raman spectroscopy. The study of mechanical strength was carried through compression tests. The biocompatibility of the composites was tested using an in-vitro cytotoxicity assay. The mechanical resistance of HAp/QC composites increased with the concentration of quasicrystalline reinforcement. Young’s modulus and compressive strength increased in 43% and 21%, respectively, with a 10 wt% QC reinforcement, which demonstrates an hybrid behaviour of the composite due to the inclusion of reinforcing particles in the pores of the matrix. This composite did not show cytotoxicity at any of the QC concentrations. A fabrication route is proposed as a fast, easy and high efficiency alternative for applications in the biomedical industry because of its high scalability potential.

In Vitro Bioactivity Study on ZnI2-HAp Nanoparticles

2020 ◽  
Vol 15 (4) ◽  
pp. 523-531
Author(s):  
Mohammad Y. Alshahrani ◽  
H. Algarni ◽  
Ibrahim AlShahrani ◽  
I. S. Yahia ◽  
H. Y. Zahran ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Sol Gel ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
Mouse Splenocytes ◽  
Fourier Transform Raman Spectroscopy ◽  
Sol Gel Process ◽  
Splenic Cells ◽  
Ft Ir

The manufactured powder of immaculate HAp which is doped with 1.0, 5.0, 10, 20, 30, and 40 in wt.% ZnI2 has been prepared via the sol–gel process. The characterization of samples was achieved by X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FT-IR) and Fourier Transform Raman spectroscopy (FT- Raman). The morphology of the obtained HAp powder was studied through SEM analysis. The investigation proves that all prepared samples have the dimension of a nanoscale. The prepared powders were examined for its anti-growth, stimulatory/cytotoxic potentials on normal/activated mouse splenocytes in vitro as well as the lytic effects on red blood cells (RBCs). Results showed that different preparations of ZnI2 had anti-growth effects on normal mouse splenocytes. The pure HAp, 1% and 10% ZnI2 showed no effects on stimulated mouse splenic cells while the other preparations 5%, 20%, 30% and 40% ZnI2 showed inhibitory/cytotoxic effects. All ZnI2 preparations showed safe effects on RBCs.

scholarly journals Synthesis, characterization and in vitro cytotoxicity study of Co and Ni ferrite nanoparticles prepared by sol-gel method

2021 ◽  
Vol 3 (7) ◽  
Author(s):  
Alexandre Pancotti ◽  
Dener Pereira Santos ◽  
Dielly Oliveira Morais ◽  
Mauro Vinícius de Barros Souza ◽  
Débora R. Lima ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Sol Gel ◽  
Test Sample ◽  
In Vitro Cytotoxicity ◽  
Gel Method ◽  
X Ray ◽  
Sol Gel Method ◽  
Transmission Electron ◽  
Crystallite Sizes

AbstractIn this study, we report the synthesis and characterization of NiFe2O4 and CoFe2O4 nanoparticles (NPs) which are widely used in the biomedical area. There is still limited knowledge how the properties of these materials are influenced by different chemical routes. In this work, we investigated the effect of heat treatment over cytotoxicity of cobalt and niquel ferrites NPs synthesized by sol-gel method. Then the samples were studied using transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), Fourier Transform Infrared Spectroscopy Analysis (FTIR), and X-ray fluorescence (XRF). The average crystallite sizes of the particles were found to be in the range of 20–35 nm. The hemocompatibility (erythrocytes and leukocytes) was checked. Cytotoxicity results were similar to those of the control test sample, therefore suggesting hemocompatibility of the tested materials.

In vitro andin vivo identification of ?pseudocatalase? activity in Dead Sea water using Fourier transform Raman spectroscopy

2002 ◽  
Vol 33 (7) ◽  
pp. 586-592 ◽  
Author(s):  
Karin U. Schallreuter ◽  
Jeremy Moore ◽  
Stefanie Behrens-Williams ◽  
Angela Panske ◽  
Marco Harari ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fourier Transform ◽  
Sea Water ◽  
Dead Sea ◽  
Fourier Transform Raman Spectroscopy ◽  
Fourier Transform Raman

Supercooled smectic nanoparticles: Influence of the matrix composition and in vitro cytotoxicity

2009 ◽  
Vol 38 (3) ◽  
pp. 238-248 ◽  
Author(s):  
Judith Kuntsche ◽  
Michel H.J. Koch ◽  
Alfred Fahr ◽  
Heike Bunjes
Keyword(s):  
In Vitro Cytotoxicity ◽  
Matrix Composition ◽  
The Matrix

scholarly journals Antibody CD133 Biofunctionalization of Ammonium Acryloyldimethyltaurate and Vinylpyrrolidone Co-Polymer-Based Coating of the Vascular Implants

Materials ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 5634
Author(s):  
Przemysław Sareło ◽  
Maciej Duda ◽  
Marlena Gąsior-Głogowska ◽  
Edyta Wysokińska ◽  
Wojciech Kałas ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Protein Molecule ◽  
Attenuated Total Reflection ◽  
In Vitro Studies ◽  
Covalent Attachment ◽  
Thiol Groups ◽  
Vascular Stents ◽  
Free Thiol ◽  
Fourier Transform Raman Spectroscopy

Current vascular stents, such as drug eluting stents (DES), have some serious drawbacks, like in stent restenosis and thrombosis. Therefore, other solutions are sought to overcome these post-implantations complications. These include the strategy of biofunctionalization of the stent surface with antibodies that facilitate adhesion of endothelial cells (ECs) or endothelial progenitor cells (EPCs). Rapid re-endothelialization of the surface minimizes the risk of possible complications. In this study, we proposed ammonium acryloyldimethyltaurate/vinylpyrrolidone co-polymer-based surface (AVC), which was mercaptosilanized in order to expose free thiol groups. The presence of free thiol groups allowed for the covalent attachment of CD133 antibodies by disulfide bridges formation between mercaptosilanized surface and cysteine of the protein molecule thiol groups. Various examinations were performed in order to validate the procedure, including attenuated total reflection–Fourier transform infrared spectroscopy (ATR-FTIR) and Fourier transform Raman spectroscopy (FT-Raman), atomic force microscopy (AFM) and scanning electron microscopy (SEM). By means of ATR-FTIR spectroscopy presence of the CD133 antibody within coating was confirmed. In vitro studies proved good biocompatibility for blood cells without induction of hemolytic response. Thus, proposed biofunctionalized CD133 antibody AVC surface has shown sufficient stability for adapting as cardiovascular implant coating and biocompatibility. According to conducted in vitro studies, the modified surface can be further tested for applications in various biological systems.

Drug Loaded Porous Hydroxyapatite and Its In Vitro Release

2005 ◽  
Vol 284-286 ◽  
pp. 423-426 ◽  
Author(s):  
Y. Kim ◽  
B.G. Song ◽  
Soo Ryong Kim ◽  
Kwang Jin Kim
Keyword(s):  
Mesoporous Silica ◽  
Body Fluid ◽  
Sol Gel ◽  
In Vitro Release ◽  
Controlled Drug Delivery ◽  
Porous Hydroxyapatite ◽  
Sol Gel Process ◽  
The Matrix ◽  
Vitro Release

Porous hydroxyapatite coated with mesoporous silica has been utilized as the matrix for controlled drug delivery. TEM observation confirms the pore size of mesoporous silica scatters about 50 Å. Porous hydroxyapatite was coated with mesoporous silica via sol-gel process. Ibuprofen and was loaded into the pores of mesoporous silica, and controlled release profiles were studied by soaking the samples in a simulated body fluid using a UV-VIS spectrophotometer.

Fractal Analysis of Tissue Biocompatible Neuroreservoir

2009 ◽  
Vol 15 ◽  
pp. 121-126 ◽  
Author(s):  
T. López ◽  
M. Patiño-Ortiz ◽  
Alexander S. Balankin ◽  
Richard D. González
Keyword(s):  
Pore Space ◽  
Sol Gel ◽  
Water Concentration ◽  
Fractal Dimensions ◽  
Electronic Microscopy ◽  
Drug Diffusion ◽  
The Matrix ◽  
Nanostructured Titania

Sol-gel nanostructured titania (TiO2) was functionalized in order to produce a nanomaterial biocompatible with brain tissue. Neuroreservoirs made from titania microtubes have been used to release sodium phenytoin and valproic acid. In vitro and in vivo probes were used to treat the epilepsy disease. Pore space topology and interactions between matrix and drug are the most important phenomena that affect the drug diffusion and liberation kinetics. Accordingly, fractal morphology of nanostructured titania as a function of pH and polarity of the drug added during the gelation reactions was studied. The transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), and N2 adsorption (BET) were employed to characterize the nanomaterials with maximum amount of air hollows occluded inside a drug. It was found that the clusters and pores in nanostructured titania are characterized by the universal fractal dimensions and . However, the characteristic sizes of particles and pores, the porosity, and the fractal dimension of pore surface ( ) are dependent on hydrolysis water concentration added during the gelation reaction. So, the matrix hydroxylation permits to control the drug liberation kinetics.

scholarly journals STUDY ON THE INFLAMMATORY RESPONSE OF PMMA/POLYSTYRENE/SILICA NANOCOMPOSITE MEMBRANES FOR DRUG DELIVERY AND DENTAL APPLICATIONS

2018 ◽  
Author(s):  
Shanmuga Sundar Saravanabhavan ◽  
KANNAN NATARAJAN ◽  
Sundaravadivel Elumalai ◽  
Sarang Zsolt ◽  
Mukunthan K SELVAM ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Sol Gel ◽  
Research Work ◽  
Polymeric Materials ◽  
Composite Membranes ◽  
In Vitro Cytotoxicity ◽  
Sem Images ◽  
Equal Concentration ◽  
Cytotoxicity Studies

Background The application of polymeric materials in medical industry has grown drastically in the last two decades due to their various advantages compared to existing materials. The present research work emphases on the sol-gel technique to formulate the polymethyl methyl acrylate/polystyrene/silica composite membrane. Methods The characteristic of the composite was investigated through modern state art of instrumentation. Results The functional groups attached to the polymer was absorbed by FTIR. The FTIR spectrum confirm that the blend was mixed thoroughly and the formation of unite intimately between the polymers. The membranes were observed by SEM for its surface homogeneity which depends upon the composition of the two blending polymers. The captured SEM images showed the formation of microcracks on the surface, which was evidently controlled by varying the constituent polymer ratios. The prepared blend membranes with 2:1 ratio of PMMA/PS/Si displayed higher water uptake compared to other blended membranes. The composite membranes had good hydroxyl apatite growth in SBF solution. Furthermore, the in vitro cytotoxicity studies carried out by MTT method, using RAW macrophage cells showed that all the samples exhibited excellent cell viability. Conclusion The inflammatory response of composite with equal concentration of PMMA-PS were performed and observed no inflammation in comparison with control and other tested concentrations.

In Vitro Cytotoxicity of Alginate-Encapsulating Ferrite Particles Using WST-1

2005 ◽  
Vol 284-286 ◽  
pp. 815-818
Author(s):  
Sang Bae Lee ◽  
Se Ho Lee ◽  
D.H. Kim ◽  
Doug Youn Lee ◽  
Yong Keun Lee ◽  
...  
Keyword(s):  
Cell Viability ◽  
Calcium Alginate ◽  
Fetal Bovine Serum ◽  
Sol Gel ◽  
In Vitro Cytotoxicity ◽  
Distilled Water ◽  
Sol Gel Process ◽  
Fetal Bovine ◽  
Alginate Content

The purpose of this study was to evaluate the cytotoxicity of alginate-encapsulting ferrite particles in vitro. Various ferrite particles such as Ba-ferrite, Sr-ferrite, Co-ferrite, Co/Ni-ferrite were prepared by sol-gel process. Ferrite particles were encapsulated via calcium alginate process with different alginate contents ranged from 10 to 100 wt%. Mouse-fibroblastic NCTC L-929 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum. The alginate-encapsulating ferrites were extracted in 5 ml of distilled water under pH 6.5 at 121°C for 1 h in accordance with ISO 10993-12. In vitro cytotoxicity was evaluated by WST-1. The results of this study indicated that the alginate-encapsulting ferrite particles affected cell viability by increasing alginate contents. Especially, alginate-encapsulating process were enhanced cell viability of ferrites such as Sr-ferrite, Co/Ni-ferrite, and Ba-Ferrite when alginate content was 10 wt%.

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