scholarly journals The Potential Use of a Thin Film Gold Electrode Modified with Laccases for the Electrochemical Detection of Pyrethroid Metabolite 3-Phenoxybenzaldehyde

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 1992
Author(s):  
Verónica Aglaeé Esquivel-Blanco ◽  
Gabriela Elizabeth Quintanilla-Villanueva ◽  
Juan Francisco Villarreal-Chiu  ◽  
José Manuel Rodríguez-Delgado ◽  
Melissa Marlene Rodríguez-Delgado
Keyword(s):  
Thin Film ◽  
Gold Electrode ◽  
Limit Of Detection ◽  
Infrared Spectrometry ◽  
Natural Environments ◽  
Direct Oxidation ◽  
Recognition Element ◽  
Electrochemical Parameters ◽  
Electrochemical Approach ◽  
Pyrethroid Pesticides

There is increasing interest in developing portable technologies to detect human health threats through hybrid materials that integrate specific bioreceptors. This work proposes an electrochemical approach for detecting 3-Phenoxybenzaldehyde (3-PBD), a biomarker for monitoring human exposure to pyrethroid pesticides. The biosensor uses laccase enzymes as an alternative recognition element by direct oxidation of 3-PBD catalysts by the enzyme onto thin-film gold electrodes. The thin-film gold electrode modified by the immobilized laccase was characterized by Fourier-transform infrared spectrometry and scanning electron microscopy. The detection method’s electrochemical parameters were established, obtaining a linear range of 5 t 50 μM, the limit of detection, and quantification of 0.061 and 2.02 μM, respectively. The proposed biosensor’s analytical performance meets the concentration of pyrethroids detected in natural environments, reflecting its potential as an alternative analytical tool for monitoring the pyrethroid insecticide’s presence.

scholarly journals Electrochemical Approach to Detection of Chlorophene in Water Catalyzed by a Laccase Modified Gold Electrode

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 82
Author(s):  
Gabriela Elizabeth Quintanilla-Villanueva ◽  
Donato Luna-Moreno ◽  
Araceli Sánchez-Álvarez ◽  
Juan Francisco Villarreal-Chiu ◽  
José Manuel Rodríguez-Delgado ◽  
...  
Keyword(s):  
Water Samples ◽  
Detection Method ◽  
Matrix Effects ◽  
Limit Of Detection ◽  
Reference Method ◽  
Health And Environmental Effects ◽  
Recognition Element ◽  
Electrochemical Parameters ◽  
Electrochemical Approach ◽  
Recovery Percentage

Despite the increasing number of reports that relate antimicrobial chlorophene (CP) with health and environmental effects, few studies have addressed biosensing technologies to detect this threat. This work proposed an electrochemical approach for the detection of CP using laccase enzymes as an alternative recognition element immobilized onto thin-film gold electrodes. The electrochemical parameters of the detection method, under controlled conditions, resulted in a limit of detection (0.14 ± 0.06 mg L−1) and quantification (0.48 ± 0.04 mg L−1) that agreed with concentrations of CP that already had been measured in natural water samples. Nevertheless, during the analysis of natural river water samples, the provided method suffered a drawback due to matrix effects reflected in the obtained recovery percentage, the value of which was 62.0 ± 2.4% compared to the 101.3 ± 3.5% obtained by the HPLC reference method. These detrimental effects were mainly attributed to organic matter, SO4-2, and Cl- present in river samples.

Electrochemical Characteristics of Aptasensor Based on MEMS Thin-Film Gold Electrode

2013 ◽  
Vol 562-565 ◽  
pp. 441-445
Author(s):  
Yan Xia Yan ◽  
Li Ying Jiang ◽  
Fen Fen Wang ◽  
Yan Zhang ◽  
Jie Hu
Keyword(s):  
Thin Film ◽  
Self Assembly ◽  
Gold Electrode ◽  
Ac Impedance ◽  
Optimum Concentration ◽  
Electrochemical Characteristics ◽  
Universal Method

A new fabrication methods of thin-film gold electrode is reported. Electrochemical characteristics of self-assembly aptasensor based on MEMS thin-film gold electrode has been studied by lots of experiments, characteristics include immobilizing time, AC impedance, CV curves. Experiments indicate immobilizing time of DNA-SH on gold electrode is more than 15 hours. CV curves and AC impedance of MEMS thin-film gold electrode show the same conclusion that 20μm/L DNA is optimum concentration when DNA is immobilized on gold electrode. Those results present a potential universal method for other aptasensors.

scholarly journals Mechanical-Resonance-Enhanced Thin-Film Magnetoelectric Heterostructures for Magnetometers, Mechanical Antennas, Tunable RF Inductors, and Filters

Materials ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2259 ◽  
Author(s):  
Cheng Tu ◽  
Zhao-Qiang Chu ◽  
Benjamin Spetzler ◽  
Patrick Hayes ◽  
Cun-Zheng Dong ◽  
...  
Keyword(s):  
Thin Film ◽  
Limit Of Detection ◽  
Rapid Development ◽  
Low Frequency ◽  
Complementary Metal Oxide Semiconductor ◽  
Cmos Technology ◽  
Medical Diagnostics ◽  
Oxide Semiconductor ◽  
Great Promise ◽  
Tunable Inductors

The strong strain-mediated magnetoelectric (ME) coupling found in thin-film ME heterostructures has attracted an ever-increasing interest and enables realization of a great number of integrated multiferroic devices, such as magnetometers, mechanical antennas, RF tunable inductors and filters. This paper first reviews the thin-film characterization techniques for both piezoelectric and magnetostrictive thin films, which are crucial in determining the strength of the ME coupling. After that, the most recent progress on various integrated multiferroic devices based on thin-film ME heterostructures are presented. In particular, rapid development of thin-film ME magnetometers has been seen over the past few years. These ultra-sensitive magnetometers exhibit extremely low limit of detection (sub-pT/Hz1/2) for low-frequency AC magnetic fields, making them potential candidates for applications of medical diagnostics. Other devices reviewed in this paper include acoustically actuated nanomechanical ME antennas with miniaturized size by 1–2 orders compared to the conventional antenna; integrated RF tunable inductors with a wide operation frequency range; integrated RF tunable bandpass filter with dual H- and E-field tunability. All these integrated multiferroic devices are compact, lightweight, power-efficient, and potentially integrable with current complementary metal oxide semiconductor (CMOS) technology, showing great promise for applications in future biomedical, wireless communication, and reconfigurable electronic systems.

Lanthanum Thin Film on Boron-Doped Diamond, Glassy Carbon and Platinum Electrodes—An Electrochemical Approach

2019 ◽  
Vol 19 (7) ◽  
pp. 4116-4122
Author(s):  
Vadivel Selvamani ◽  
Venkatachalam Rajagopal ◽  
Noel Nesakumar ◽  
David Velayutham ◽  
Vembu Suryanarayanan
Keyword(s):  
Thin Film ◽  
Glassy Carbon ◽  
Platinum Electrodes ◽  
Boron Doped Diamond ◽  
Boron Doped ◽  
Electrochemical Approach

scholarly journals Distribution of Pesticides in Different Commonly Grown Vegetables of Cameron Highlands, Pahang, Malaysia

2021 ◽  
Vol 50 (10) ◽  
pp. 2937-2944
Author(s):  
Nusrat Munawar ◽  
Yang Farina Yang Farina ◽  
Mohammad Yaqoob ◽  
Abdul Nabi Abdul Nabi ◽  
Syed Munawar Shah Syed Munawar Shah
Keyword(s):  
Gas Chromatography ◽  
Limit Of Detection ◽  
Organophosphorus Pesticides ◽  
Pca Analysis ◽  
Wet Season ◽  
Vegetable Samples ◽  
Detection Frequency ◽  
Relative Standard ◽  
Pyrethroid Pesticides ◽  
Cameron Highlands

Gas chromatography-electron capture detector (GC-ECD) is used to extract and analyse pesticides in vegetable samples collected from Cameron Highlands, Pahang, Malaysia. The limit of detection (LOD) for all pesticides was in the range of 0.03 to 4.5 ng g-1. Recoveries in cabbage, lettuce, and celery ranged from 61.8%-121%, 60-128% and 60%-114%, respectively. The relative standard deviation (RSD) ranged 0.2-15% in cabbage, 0.5-18% in lettuce and 3-19.8% in celery. Organochlorine pesticides (OCPs) concentrations increased down the valley with dichlorodiphenyldichloroethylene (DDE) having the highest concentration at 233 µg kg-1. Organophosphorus pesticides (OPPs) were found to be dispersed throughout the valley, with the highest concentration of parathion ethyl (133 µg kg-1) whereas the pyrethroid pesticides (PYRs) concentrations were comparatively less. The detection frequency in the wet season was highest (5 < - < 100 µg kg-1) for most pesticides. However, in the dry season the pesticides concentrations were higher, at < 5 µg kg-1. PCA analysis indicated that farmers were using a mixture of pesticides.

scholarly journals Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 115
Author(s):  
Jorge Lopez-Tellez ◽  
Irais Sanchez-Ortega ◽  
Claudia Teresa Hornung-Leoni ◽  
Eva Maria Santos ◽  
Jose Manuel Miranda ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gold Electrode ◽  
Detection Method ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Salmonella Spp ◽  
Analysis Methodology ◽  
Nyquist Plots ◽  
Impedimetric Biosensor ◽  
Hen Egg

A sensitive electrochemical detection method for Salmonella spp. was described, based on the use of Hechtia argentea lectin immobilised on a screen-printed gold electrode. The lectin was extracted from Hechtia argentea, a plant belonging to the Bromeliaceae family. The lectin with molecular weight near 27.4 kDa showed selectivity towards D-mannose, contained on the lipopolysaccharide cell wall of Salmonella spp. Carbohydrate selectivity of the lectin was measured as a change in impedance with respect to concentration. The binding of the bacteria to the biosensor surface increased impedance with increasing concentrations of Salmonella spp., achieving a linear range of detection of 15–2.57 × 107 CFU mL−1, with a limit of detection of 5 CFU mL−1. Increases in impedance were measured using electrochemical impedance spectroscopy and analysed using Nyquist plots. The biosensor was applied in analysis of hen egg samples, and the results were consistent with those obtained using the official analysis methodology.

scholarly journals An Electrochemical Sensor based on Electrodeposited CTAB Film on Glassy Carbon Electrode for Detection of Morphine

2019 ◽  
Vol 32 (1) ◽  
pp. 137-141
Author(s):  
Pinky Abraham ◽  
S. Renjini ◽  
V. Anithakumary ◽  
P.G. Chithra
Keyword(s):  
Thin Film ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Sensing Platform ◽  
Excellent Electrochemical Performance ◽  
Tertiary Amino ◽  
Pulse Voltammetry ◽  
Glassy Carbon Modified Electrode

A simple, effective and rapid method for the electrochemical detection of morphine is described based on glassy carbon modified electrode with poly(CTAB). In this work, poly(CTAB) thin film was generated through elecropolymerization of the surfactant CTAB. The formation of nanoporous thin film of poly(CTAB) was confirmed by field emission scanning electron microscopy (FESEM) with energy dispersive spectra (EDS) and Fourier transform infrared spectroscopy (FTIR). The electrochemical behavior of morphine is explained in terms of the anodic oxidation of its tertiary amino group. The limit of detection was calculated as 0.2 μM with a good regression between concentration and peak current of morphine by using differential pulse voltammetry within the range of 50 nM to 20 μM. The poly(CTAB)/GCE based sensor shows excellent electrochemical performance for the detection of morphine and this sensing platform can be effective for the detection of similar molecules.

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