scholarly journals An In Vitro Stereomicroscopic Evaluation of Bioactivity between Neo MTA Plus, Pro Root MTA, BIODENTINE & Glass Ionomer Cement Using Dye Penetration Method

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3159
Author(s):  
Mohmed Isaqali Karobari ◽  
Syed Nahid Basheer ◽  
Fazlur Rahman Sayed ◽  
Sufiyan Shaikh ◽  
Muhammad Atif Saleem Agwan ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Root Canal ◽  
Glass Ionomer Cement ◽  
Filling Material ◽  
Glass Ionomer ◽  
Dye Penetration ◽  
Anterior Teeth ◽  
Significant Difference ◽  
Ionomer Cement

The ideal root end filling material should form a tight seal in the root canal by adhering to the cavity walls. Several materials have been used for root end filling. The present study aims to find out and compare the bioactivity of Neo MTA Plus, Pro Root MTA White, BIODENTINE & glass ionomer cement as root end filling materials using 1% methylene blue as tracer. Materials and methods: 80 extracted human permanent maxillary anterior teeth were used in the study. They were divided into four groups. Specimens were sectioned transversely in the cervical area to separate the crown from the root. The root canal was obturated with gutta percha and zinc oxide eugenol sealers. Thereafter, each sample was resected apically by removing 3 mm of the apex and filled with different materials. Samples were kept in buffering solution at 37 °C until the recommended evaluation periods. The specimens were then suspended in 1% methylene blue for 24 h, prior to the analysis. The teeth were then sectioned, and dye penetration was examined, photographed, and evaluated under a stereomicroscope. Results: Vertical dye penetration showed significant differences across different groups. The minimum dye penetration was seen in Neo MTA plus followed by BIODENTINE, Pro Root MTA and maximum in GIC. There was no significant difference in dye penetration between Neo MTA plus and BIODENTINE both at fifteen days and one-month intervals. Conclusion: The present study suggests Neo MTA plus and BIODENTINE should be the preferred material for root end filling.

scholarly journals In vitro quantitative evaluation of marginal microleakage in class II restorations confected with a glass ionomer cement and two composite resins

2001 ◽  
Vol 15 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Maria Fernanda Borro BIJELLA ◽  
Maria Francisca Thereza Borro BIJELLA ◽  
Salete Moura Bonifácio da SILVA
Keyword(s):  
Glass Ionomer Cement ◽  
Class Ii ◽  
Deionized Water ◽  
Composite Resins ◽  
Glass Ionomer ◽  
Dye Penetration ◽  
Significant Difference ◽  
Distal Direction ◽  
Ionomer Cement

This study evaluated, in vitro, marginal microleakage in class II restorations confected with the glass ionomer cement Vitremer and with the composite resins Ariston pHc and P-60. The aims of the study were to assess the effect of thermocycling on those materials and to evaluate two methods utilized in the analysis of dye penetration. Sixty premolars divided in three groups were utilized; the teeth had proximal cavities whose cervical walls were located 1 mm below the cementoenamel junction. Half of the test specimens from each group underwent thermocycling; the other half remained in deionized water, at 37ºC. The specimens were immersed, for 24 hours, in a basic 0.5% fuchsin solution at 37ºC. For the analysis of microleakage, the specimens were sectioned in a mesio-distal direction, and the observation was carried out with the software Imagetools. The results were evaluated through the 2-way ANOVA and through the Tukey’s test. All groups presented marginal microleakage. The smallest values were obtained with Vitremer, followed by those obtained with the composite resins P-60 and Ariston pHc. There was no statistically significant difference caused by thermocycling, and the method of maximum infiltration was the best for detecting the extension of microleakage.

Antibacterial Activity of Triclosan Incorporated Glass Ionomer Cements – An in vitro Pilot Study

2010 ◽  
Vol 35 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Sajad Sainulabdeen ◽  
Prasanna Neelakantan ◽  
Sindhu Ramesh ◽  
CV Subbarao
Keyword(s):  
Antibacterial Activity ◽  
Pilot Study ◽  
Glass Ionomer Cement ◽  
Diffusion Method ◽  
Control Group ◽  
Glass Ionomer ◽  
Group I ◽  
Significant Difference ◽  
Ionomer Cement

Objectives: The aim of this pilot study was to evaluate the antibacterial activity of glass ionomer cement impregnated with different concentrations (0.5%, 1.25% and 2.5%) of a non releasing bactericide –Triclosan (TC) against two common cariogenic bacteria – Lactobacillus acidophilus and Streptococcus mutans; and to compare Triclosan incorporated GIC with chlorhexidine (CHX) incorporated GIC (2.5%)in terms of antibacterial activity. Methods: Chlorhexidine or Triclosan were added to glass ionomer cement powder to achieve 2.5% CHX – GIC (positive control – Group II), 0.5%, 1.25% and 2.5% TC-GIC (experimental groups III, IV and V respectively) formulations. Restorative glass ionomer cement (Fuji IX GC –Group I) served as negative control. The powder and liquid were mixed and inserted into the wells punched in agar plates (10mm × 4mm). The agar diffusion method was used to determine the antibacterial activity of the cements after 1, 7 and 30 days. Mean values were compared between different study groups using One-way ANOVA and Tukey's HSD procedure at a significance level of 5%. Results: Triclosan incorporated GIC was more effective against L.acidophilus and S.mutans than Chlorhexidine incorporated GIC. Triclosan at a concentration of 2.5% was more effective than at lower concentrations. At all time periods studied, the maximum zone of inhibition against L.acidophilus was produced by Group V. Against S.mutans, on days 1,7 and 30, there was no significant difference between Groups II and IV (p>0.05), while the other groups showed significant differences. Conclusion: The use of triclosan as an antibacterial additive in GIC holds promise and further clinical research is needed in this direction.

scholarly journals In vitro evaluation of the marginal microleakage of amalgam restorations associated with dentin adhesive, glass ionomer cement and cavity varnish by means of different evaluation methods

2005 ◽  
Vol 13 (1) ◽  
pp. 10-14
Author(s):  
Adriano Tomio Hoshi ◽  
Salete Moura Bonifácio da Silva ◽  
Aymar Pavarini
Keyword(s):  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Evaluation Methods ◽  
Single Line ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Amalgam Restorations ◽  
Ionomer Cement ◽  
Dentin Adhesive

This in vitro study evaluated the marginal microleakage of amalgam restorations associated with the cavity varnish Copalite - Cooley & Cooley (GI-CP), dentin adhesive OptiBond Solo - Kerr (GII-OS) and the glass ionomer cement Vitremer - 3M (GIII-VT). Forty-five premolars were employed, which were submitted to independent class II preparations at the mesial and distal aspects comprising the marginal ridges and were restored with Dispersalloy - Dentsply. Afterwards, the teeth were thermocycled and stored in 0.5% basic fuchsine for 24 hours. The evaluations were conducted on a light microscope with 150x magnification and on the Sigma Scan software with employment of a single line and segmented lines. Data analysis allowed to establish that none of the materials was able to eliminate the marginal microleakage, having the GI - CP presented greater and statistically significant values in relation to the other groups in all evaluation methods (p<0.05). The lowest values were displayed by the GIII-VT, yet with no statistically significant difference when compared to GII-OS, except for the evaluation at the Sigma Scan in single line. The three evaluation methods showed a strong positive relationship to each other.

scholarly journals An Evaluation on Sealing Ability of Calcium Silicate-Based Cements and Glass Ionomer Cement as Perforation Repair Materials

2021 ◽  
pp. 137-145
Author(s):  
Vanishree H. Shivakumar ◽  
Anand S. Tegginamani ◽  
Daniel Devaprakash Dicksit ◽  
Ahmad Termizi B Zamzuri
Keyword(s):  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Glass Ionomer ◽  
Penetration Length ◽  
Dye Penetration ◽  
Sealing Ability ◽  
Repair Materials ◽  
Group B ◽  
Ionomer Cement

Aims: The study evaluated the sealing ability of Biodentine, MTA Repair HP, and Glass ionomer cement as perforation repair materials by using a Stereomicroscopic analysis. Study Design: Experimental in vitro study Methodology: The access cavity was prepared on 45 samples of maxillary and mandibular teeth with a perforation of the standardized diameter of a No. 2 round bur at the bottom of the pulp chamber. All 45 samples were divided into three different experimental groups of 15 samples each. Group A (n=15), Group B (n=15) and Group C (n=15). The furcation repairs of the samples in groups A, B and C were carried out using Biodentine, MTA Repair HP and glass ionomer cement respectively. All sealed furcation perforation samples were stored at room temperature for 24 hours. Two layers of nail varnish were coated on all the surfaces to avoid dye penetration except for 2 mm around the area of the perforation site. After complete drying, all specimens were separately soaked in 2% methylene blue solution for 48 hours, cleaned with water and dried for 24 hours. They were sectioned buccolingually. The perforation wall of the sectioned sample with the greatest dye penetration was selected for microleakage analysis. Results: The collected data from the three experimental groups were subjected to statistical analysis using one-way analysis of variance and Tukey's post hoc test for multiple comparisons of mean differences in dye penetration. The Biodentine group had the significantly lowest dye penetration length compared with the MTA Repair HP and glass ionomer cement groups (P<0.001). Conclusion: Biodentine showed better sealing ability as a repair material for furcation perforations compared to the other two materials.

In vitro Biocompatibility Tests of Glass Ionomer Cements Impregnated with Collagen or Bioactive glass to Fibroblasts

2012 ◽  
Vol 36 (3) ◽  
pp. 269-274 ◽  
Author(s):  
C Subbarao ◽  
P Neelakantan ◽  
CV Subbarao
Keyword(s):  
Bioactive Glass ◽  
Cell Attachment ◽  
Glass Ionomer Cement ◽  
Mineral Trioxide Aggregate ◽  
Fibroblast Cell ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Electron Microscopes ◽  
Ionomer Cement

Aim and Design: To evaluate the biocompatibility of glass ionomer cement (GIC) impregnated with collagen or bioactive glass to BHK-21 fibroblasts in vitro. Mineral Trioxide Aggregate was used as the standard for comparison. Human maxillary central incisors (n=70) were instrumented with a rotary NiTi system and filled. Following resection of the apical 3mm, root end cavities were prepared and restored with conventional GIC (group 1) or GIC with 0.01%, 0.1% or 1% collagen (groups 2, 3, 4 respectively) or, 10%, 30 % or 50% bioactive glass (groups 5,6,7 respectively), or Mineral Trioxide Aggregate (group 8). The root slices were incubated in tissue culture plates with BHK-21 fibroblast cell line. Phase contrast and scanning electron microscopes were used to score cell quantity, morphology and cell attachment. The data were statistically analyzed by one way ANOVA with Post Hoc Tukey HSD test (p = 0.05). Results and conclusions: Group 5 showed the highest scores which was significantly higher than all other groups (p&lt;0.05) except group 8, with which there was no significant difference (p&gt;0.05). Glass ionomer cement with 10% bioactive glass showed better adhesion and spreading of cells than glass ionomer cement with 0.01% collagen. The biocompatibility of collagen and bioactive glass was concentration dependent. The addition of bio active glass improved the biocompatibility of glass ionomer cement to fibroblasts better than addition of collagen.

scholarly journals Microleakage under orthodontic bands cemented with nano-hydroxyapatite-modified glass ionomer: An in vivo study

2013 ◽  
Vol 83 (6) ◽  
pp. 981-986 ◽  
Author(s):  
Enas T. Enan ◽  
Shaza M. Hammad
Keyword(s):  
Statistical Evaluation ◽  
Glass Ionomer Cement ◽  
Glass Ionomer ◽  
Significance Level ◽  
Dye Penetration ◽  
Significant Difference ◽  
Orthodontic Patients ◽  
Positive Effect ◽  
Ionomer Cement

ABSTRACT Objectives: To estimate the in vivo effect of nano-hydroxyapatite (HA) modification of banding glass-ionomer cement on microleakage under orthodontic bands. Materials and Methods: Eighty noncarious premolars scheduled for extraction in 20 orthodontic patients were randomly divided into four groups. Grouping was based on the ratio of nano-HA (0%, 5%, 10%, 15% by weight) added to the luting glass-ionomer cement (GIC) Ketac-Cem, which was used for cementation of prefabricated micro-etched orthodontic bands. Dye penetration method was used for microleakage evaluation at the cement-band and cement-enamel interfaces. Statistical evaluation was performed with a Kruskal-Wallis test and a Mann-Whitney U-test, and a Bonferroni-adjusted significance level was calculated. Results: Bands cemented with conventional GIC showed the highest microleakage scores in comparison to those cemented with nano-HA-modified GIC. No significant difference was found between teeth banded with 10% and 15% modified GIC. Conclusions: Modification of the banding GIC with 15% nano-HA revealed a positive effect on reducing microleakage around orthodontic bands.

scholarly journals Sealing Ability of Three Different Surface Coating Materials on Conventional and Resin Modified Glass Ionomer Restoration in Primary Anterior Teeth: An In vitro Study

2021 ◽  
Author(s):  
Anshula Deshpande ◽  
Chirag Macwan ◽  
Steffi Dhillon ◽  
Medha Wadhwa ◽  
Neelam Joshi ◽  
...  
Keyword(s):  
Surface Coating ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Glass Ionomer ◽  
Coating Materials ◽  
Anterior Teeth ◽  
Group 2 ◽  
Ionomer Cement ◽  
Group 1

Introduction: Microleakage is an important property that has been used in assessing the success of any restorative material used in restoring a tooth. Immediate application of a surface coating agent is suggested to protect glass ionomer cement against moisture contamination and dehydration during early setting. Aim: To compare marginal microleakage of two different Glass Ionomer Cement (GIC)- Conventional GIC and Resin Modified GIC in primary anterior teeth using three surface coating materials. Materials and Methods: An in vitro study was conducted between January 2014 to October 2017 on freshly extracted 40 anterior primary teeth which were randomly assigned into two main groups (Fuji II GIC and Fuji II LC GIC) with four subgroups (control-no surface coating, A=GC Fují Varnish II, B=GC G-Coat Plus, C=Icon). A standardised Class V cavity preparation was prepared on the labial surface of each tooth. Specimens were coated with two layers of nail varnish, leaving a 1 mm window around the cavity margins and placed in a solution of Methylene blue Dye for 24 hour at 37°C. The teeth were sectioned longitudinally in a buccolingual direction of the restorations and evaluated under stereomicroscope to check extent of dye penetration. The results were analysed by ANOVA and Tukey’s post-hoc test (p≤0.05). Results: It was found that maximum microleakage was seen in group 1 (Fuji II GIC) as compared to group 2 (Fuji II LC GIC) and it was non-significant (p=0.53). Ascending order for mean microleakage for Group 1 was as follows: Control >GC Fuji Varnish >Icon DMG >GC G-Coat and for Group 2: Control >GC Fuji Varnish >Icon DMG >GC G-Coat. Icon when compared with Gc coat and Varnish also showed non-significant (p=0.137) difference in Group 2. Conclusion: All three different surface coatings can seal glass ionomer restorations. The GC G-Coat Plus has the least microleakage on Resin-Modified GIC (RMGIC) compared to the other surface coatings. This would aid the clinicians to make appropriate decision regarding the choice of material to be used for restoration and coating in anterior primary teeth.

scholarly journals PERBANDINGAN KEBOCORAN MIKRO PADA RESTORASI RESIN KOMPOSIT MIKROFILLER DENGAN RESIN-MODIFIED GLASS IONOMER CEMENT (RMGIC) PADA KAVITAS KLAS V GIGI ANTERIOR

2015 ◽  
Vol 3 (2) ◽  
pp. 83-91
Author(s):  
Thesi Kurnia Ayudia ◽  
Kuswardani Susari Putri ◽  
Ivony Fitria
Keyword(s):  
Composite Resin ◽  
Glass Ionomer Cement ◽  
Base Material ◽  
Glass Ionomer ◽  
Class V ◽  
Anterior Teeth ◽  
Group I ◽  
Resin Modified Glass Ionomer ◽  
Ionomer Cement

Microleakage defined as the clinically undetectable passage of bacteria, fluids, molecules or ions between a cavity wall and the restorative material. Microleakage tends to occur in Class V cavities. It is caused by  marginal adaptation  which is more difficult in class  V cavities. Microfiller composite resin is developed and indicated for areas that not require a large pressure. As the development of dental materials, it has been developed a composite resin base material that is known as resin-modified glass ionomer cement. This material has purposed to reduce the limitation of conventional glass ionomer cement and take the advantage of the composite resin material. The aim of this study was   to evaluate microleakage difference of microfiler composite resin restoration with resin-modified glass ionomer cement restorations in class V anterior teeth cavities.  The methode of this  study used experimental laboratory through in vitro process . Thirty two class V cavities were prepared on labial surfaces of extracted human anterior teeth. Samples were divided into two groups. Group I included sixteen samples that have had   restorated with microfiller composite. Group II included sixteen samples that have had restorated with resin-modified glass ionomer cement. The samples were immersed into aquabides solution for 24 hours. After that, the samples were immersed into 1% methylene blue solution for 24 hours. All samples sectioned longitudinally and analyzed for microleakage as dye penetration using a stereomicroscope. Student t-test were used for statistical analysis. The resulting data showed no significantly difference between two groups. Key Word : Microleakage, microfiller composite resin, resin-modified glass ionomer cement (RMGIC), class V cavities.

Evaluation of Microleakage of Orthodontic Bands Cemented With CPP-ACP-Modified Glass Ionomer Cement

2019 ◽  
Vol 10 (2) ◽  
pp. 128-131
Author(s):  
Farzin Heravi ◽  
Hossein Bagheri ◽  
Abdolrasoul Rangrazi
Keyword(s):  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Glass Ionomer ◽  
Fluid Filtration ◽  
Filtration Method ◽  
Significant Difference ◽  
Patient Cooperation ◽  
Group 2 ◽  
Ionomer Cement

Aims and Objectives: In recent years, researchers have focused on finding methods of eliminating caries and white spot lesions under orthodontic bands without requiring patient cooperation. One of the effective and preventive strategies is the addition of antibacterial and remineralizing agents to the band cement. However, modifying the cement should not lead to microleakage and weakening of its mechanical properties. This in vitro study represents a continuation of our previous work of investigating the retentive strength of orthodontic bands cemented with casein phosphopeptide-amorphous calcium phosphate (CPP-ACP)-modified glass ionomer cement (GIC). Material and Methods: 60 extracted human premolars were randomly divided into two groups (n = 30). In group 1, stainless steel bands were cemented with GIC. In group 2, a novel dental caries-preventive material, CPP-ACP, was incorporated into the GIC for cementing. The microleakage of each group was measured by the fluid filtration method. Results: Results showed no statistically significant difference in microleakage of either group (GIC and CPP-ACP-modified GIC). Conclusion: We concluded that 1.56% w/w CPP-ACP can be incorporated with GIC for cementing orthodontic bands without promoting microleakage.

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