scholarly journals Antibacterial Activity of Long-Chain Polyunsaturated Fatty Acids against Propionibacterium acnes and Staphylococcus aureus

Marine Drugs ◽  
2013 ◽  
Vol 11 (11) ◽  
pp. 4544-4557 ◽  
Author(s):  
Andrew Desbois ◽  
Keelan Lawlor
Author(s):  
Ika Kurnia Sukmawati ◽  
Ari Yuniarto ◽  
Widhya Alighita ◽  
Ade Zam-zam J

Acne is an inflammatory disease that occurs in the skin triggered by the bacteria acne treatment can be done by using natural materials that shiitake mushrooms (Lentinus edodes). This aims of this study were to determining the antibacterial activity of extracts and fractions shiitake mushrooms with broth microdilution method, determining the value of equality shiitake mushrooms with antibacterial comparison and determining the morphological changes of bacteria after exposure to the test sample with a Scanning Electron Microscope (SEM). Tests conducted antibacterial activity against Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at concentrations used is 1 ppm, 2 ppm, 4 ppm, 8 ppm, 16 ppm, 32 ppm, 64 ppm, 128 ppm, 256 ppm, 512 ppm. Best MIC value obtained in fraction of ethyl acetate and n-hexane fraction of the bacterium Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at a concentration of 256 ppm. KBM value of the n-hexane fraction against Staphylococus aureus bacteria at concentrations of 512 ppm and ethyl acetate fraction against bacteria Staphylococus aureus and epidermidis at a concentration of 512 ppm. Value equality is obtained 1 mg of ethyl acetate fraction of shiitake mushrooms equivalent to 5.346 x 10-2 mg of tetracycline. SEM test results showed the presence of antibacterial activity which is indicated by a change in cell morphology, their lumps and their cell wall frown on Propionibacterium acnes were exposed to ethyl acetate fraction.Key words: Acne, Lentinus edodes, antibacterials, microdilution, SEM


Author(s):  
Cici Nasya Nita ◽  
Rosha Kurnia Fembriyanto ◽  
Nur Annis Hidayati

Kayu lubang are plants which one traditionally used to acne treatment. Scientifically acne is caused bacteria’s such as Propionibacterium acnes dan Staphylococcus aureus infection. The growth of Propionibacterium acnes and Staphylococcus aureus be inhibition with phytochemical compounds contained leaves of kayu lubang. The purpose of this research was to identification phytochemical compunds from rude extract ethanol leaves of kayu lubang and which consentration extract that shows the optimum to inhibition growth of Propionibacterium acnes dan Staphylococcus aureus in antibacterial activity test. Phytochemical test conducted qualitative and antibacterial activity test using disc diffusion with concentration 15%, 20%, 25%, 30%, 35% dan 40% in 3 replication. Based on the results showed that in general those leaves contain alcaloid, phenol, tannin and steroid. The optimum inhibition zone results of extract  inhibition bacterial growth at concentration of 40%,  inhibition zone Propionibacterium acnes inhibition zone of 6,83 mm while Staphylococcus aureus bacteria was 5,53  mm. Based on the results of statistical tests DMRT effects of extracts leaves of kayu lubang  against the bacteria Propionibacterium acnes  and  Staphylococcus aureus showed significant differences among for 95% confidence.


2006 ◽  
Vol 60 (4) ◽  
pp. 485-489 ◽  
Author(s):  
Silvia Scaglioni ◽  
Elvira Verduci ◽  
Michela Salvioni ◽  
Maria Luisa Biondi ◽  
Giovanni Radaelli ◽  
...  

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1220
Author(s):  
Eduard V. Nekrasov ◽  
Vasily I. Svetashev

Young fronds of ferns are consumed as a vegetable in many countries. The aim of this study was to analyze three fern species that are available for sale in the Russian Far East as dietary sources in terms of fatty acids that are important for human physiology: arachidonic acid (20:4n-6, ARA), eicosapentaenoic acid (20:5n-3, EPA) and other valuable long-chain polyunsaturated fatty acids. The content of ARA and EPA was 5.5 and 0.5 mg/g dry weight, respectively, in Pteridium aquilinum, 4.1 and 1.1 in Matteuccia struthiopteris, and 2.2 and 0.8 in Osmundastrum asiaticum. Salted fronds of P. aquilinum contained less these fatty acids than the raw fronds, with a decrease of up to 49% for ARA and 65% for EPA. These losses were less pronounced or even insignificant in dried fronds. Cooked ferns preserved significant portions of the long-chain polyunsaturated fatty acids: cooked P. aquilinum contained 4.4 mg/g dry weight ARA and 0.3 mg/g dry weight EPA. The ferns may provide a supplemental dietary source of these valuable long-chain polyunsaturated fatty acids, especially for vegetarian diets.


Meat Science ◽  
2021 ◽  
Vol 177 ◽  
pp. 108497
Author(s):  
Juan D. Rios-Mera ◽  
Erick Saldaña ◽  
Iliani Patinho ◽  
Miriam M. Selani ◽  
Carmen J. Contreras-Castillo

2014 ◽  
Vol 62 (41) ◽  
pp. 10153-10161 ◽  
Author(s):  
Madlen Stimming ◽  
Christina M. Mesch ◽  
Mathilde Kersting ◽  
Hermann Kalhoff ◽  
Hans Demmelmair ◽  
...  

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2453
Author(s):  
Ana M Pinto ◽  
Helen L MacLaughlin ◽  
Wendy L Hall

Low heart rate variability (HRV) is independently associated with increased risk of sudden cardiac death (SCD) and all cardiac death in haemodialysis patients. Long chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) may exert anti-arrhythmic effects. This study aimed to investigate relationships between dialysis, sleep and 24 h HRV and LC n-3 PUFA status in patients who have recently commenced haemodialysis. A cross-sectional study was conducted in adults aged 40–80 with chronic kidney disease (CKD) stage 5 (n = 45, mean age 58, SD 9, 20 females and 25 males, 39% with type 2 diabetes). Pre-dialysis blood samples were taken to measure erythrocyte and plasma fatty acid composition (wt % fatty acids). Mean erythrocyte omega-3 index was not associated with HRV following adjustment for age, BMI and use of β-blocker medication. Higher ratios of erythrocyte eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) were associated with lower 24 h vagally-mediated beat-to-beat HRV parameters. Higher plasma EPA and docosapentaenoic acid (DPAn-3) were also associated with lower sleep-time and 24 h beat-to-beat variability. In contrast, higher plasma EPA was significantly related to higher overall and longer phase components of 24 h HRV. Further investigation is required to investigate whether patients commencing haemodialysis may have compromised conversion of EPA to DHA, which may impair vagally-mediated regulation of cardiac autonomic function, increasing risk of SCD.


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