scholarly journals N-Acetyl-d-Glucosamine-Binding Lectin in Acropora tenuis Attracts Specific Symbiodiniaceae Cell Culture Strains

Marine Drugs ◽  
2021 ◽  
Vol 19 (3) ◽  
pp. 146
Author(s):  
Ryota Takeuchi ◽  
Mitsuru Jimbo ◽  
Fumika Tanimoto ◽  
Mariko Iijima ◽  
Hiroshi Yamashita ◽  
...  
Keyword(s):  
Cell Culture ◽  
Water Column ◽  
Chemotactic Activity ◽  
Juvenile Polyps ◽  
Acropora Tenuis ◽  
Cell Strains ◽  
Binding Lectin

Many corals establish symbiosis with Symbiodiniaceae cells from surrounding environments, but very few Symbiodiniaceae cells exist in the water column. Given that the N-acetyl-d-glucosamine-binding lectin ActL attracts Symbiodiniaceae cells, we hypothesized that corals must attract Symbiodiniaceae cells using ActL to acquire them. Anti-ActL antibody inhibited acquisition of Symbiodiniaceae cells, and rearing seawater for juvenile Acropora tenuis contained ActL, suggesting that juvenile A. tenuis discharge ActL to attract these cells. Among eight Symbiodiniaceae cultured strains, ActL attracted NBRC102920 (Symbiodinium tridacnidorum) most strongly followed by CS-161 (Symbiodinium tridacnidorum), CCMP2556 (Durusdinium trenchii), and CCMP1633 (Breviolum sp.); however, it did not attract GTP-A6-Sy (Symbiodinium natans), CCMP421 (Effrenium voratum), FKM0207 (Fugacium sp.), and CS-156 (Fugacium sp.). Juvenile polyps of A. tenuis acquired limited Symbiodiniaceae cell strains, and the number of acquired Symbiodiniaceae cells in a polyp also differed from each other. The number of Symbiodiniaceae cells acquired by juvenile polyps of A. tenuis was correlated with the ActL chemotactic activity. Thus, ActL could be used to attract select Symbiodiniaceae cells and help Symbiodiniaceae cell acquisition in juvenile polyps of A. tenuis, facilitating establishment of symbiosis between A. tenuis and Symbiodiniaceae cells.

Neuraminic Acid Investigations of Human Cell Strains derived from Explants of Skin in Cell Culture

Nature ◽  
1964 ◽  
Vol 201 (4923) ◽  
pp. 1050-1051 ◽  
Author(s):  
ROBEBT M. EIBEN ◽  
STANLEY M. GARTLER
Keyword(s):  
Cell Culture ◽  
Human Cell ◽  
Neuraminic Acid ◽  
Cell Strains

Differential gene expression in juvenile polyps of the coral Acropora tenuis exposed to thermal and chemical stresses

2012 ◽  
Vol 430-431 ◽  
pp. 17-24 ◽  
Author(s):  
Ikuko Yuyama ◽  
Yoshihiko Ito ◽  
Toshiki Watanabe ◽  
Michio Hidaka ◽  
Yoshimi Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Juvenile Polyps ◽  
Acropora Tenuis ◽  
Differential Gene

scholarly journals Spontaneous and Induced Changeableness and Accumulation of Phenol Compounds in Cell Culture of Alhagi kirghisorum

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 875A-875
Author(s):  
O.A. Sapko ◽  
Z.B. Shamina
Keyword(s):  
Cell Culture ◽  
Protein Biosynthesis ◽  
Resistant Cell ◽  
Activity Maximum ◽  
Phenol Compounds ◽  
Phenol Compound ◽  
Cell Strains ◽  
High Level ◽  
Rabbit Reticulocytes

Our studies concerned the peculiarities of phenol compound (PhC) formation in cultivated in vitro cells of Alhagi kirghisorum. To isolate cell strains with a high level of PhC biosynthesis, cells were sown on a medium containing parafluorophenylalanine (PFPA). To increase the number of resistant cell lines, the cells were treated with N-nitrose-N-methylurea (NMU). Four groups of individual clones were obtained: spontaneous, induced NMU, and resistant to PFPA. These clones differed in their intensity of growth, color, consistence, quantity, and PhC composition, as well as in their biological activity. Maximum differences were found in the clones with induced resistivity. In this group, clones with PhC content were at the level of control cells (40%), with high (33%) and low level of biosynthesis (27%). The PhC content in two more productive lines was 3.3 and 4.2 times higher than in controls. The induced NMU clones and clones with spontaneous resistivity to PFPA had biosynthesis levels similar to the control or 3 to 4 times lower than the latter. The biological PhC activity of a clone was tested by its effect on the processes of protein biosynthesis in a system without cells from rabbit reticulocytes. Eleven clones were found, the total PhC fractions of which in 40% to 99% inhibited protein biosynthesis.

scholarly journals Establishing Sustainable Cell Lines of a Coral, Acropora tenuis

2021 ◽  
Author(s):  
Kaz Kawamura ◽  
Koki Nishitsuji ◽  
Eiichi Shoguchi ◽  
Shigeki Fujiwara ◽  
Noriyuki Satoh
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Cell Lines ◽  
Culture Medium ◽  
Single Cells ◽  
Secretory Cells ◽  
Developmental Potential ◽  
Acropora Tenuis ◽  
In Vitro Cell Lines

AbstractPlanula larvae of the scleractinian coral,Acropora tenuis, consist of elongated ectodermal cells and developing inner endodermal cells. To establish in vitro cell lines for future studies of cellular and developmental potential of coral cells, larvae were successfully dissociated into single cells by treating them with a tissue dissociation solution consisting of trypsin, EDTA, and collagenase. Brown-colored cells, translucent cells, and pale blue cells were the major components of dissociated larvae. Brown-colored cells began to proliferate transiently in the culture medium that was devised for the coral, while translucent cells and pale blue cells decreased in number about 1 week after cell dissociation. In addition, when a modular protease, plasmin, was added to the cell culture medium, brown-colored cells extended pseudopodia and assumed amorphous shapes. They then continued to proliferate in clumps for more than 6 months with a doubling time of approximately 4–5 days. From 3 weeks of cell culture onward, brown-colored cells often aggregated and exhibited morphogenesis-like behavior to form flat sheets, and blastula-like clusters or gastrula-like spheres. Single cells or cell-clusters of the cell lines were analyzed by RNA-seq. This analysis showed that genes expressed in these cells in vitro wereA. tenuisgenes. Furthermore, each cell line expressed a specific set of genes, suggesting that their properties include gastroderm, secretory cells, undifferentiated cells, neuronal cells, and epidermis. All cell properties were maintained stably throughout successive cell cultures. These results confirm the successful establishment of a coral in vitro cell line.

A T.E.M. study of mouse mammary epithelial cell secretory differentiation cultured on collagen and Matrigel

1991 ◽  
Vol 49 ◽  
pp. 188-189
Author(s):  
W.N. Bentham ◽  
V. Rocha
Keyword(s):  
Cell Culture ◽  
Mammary Epithelial ◽  
Secretory Process ◽  
Cell Culture System ◽  
Protein Maturation ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Mouse Mammary Epithelial Cell ◽  
Secretory Differentiation

It has been an interest of our lab to develop a mammary epethelial cell culture system that faithfully duplicates the in vivo condition of the lactating gland. Since the introduction of collagen as a matrix on which cells are cultivated other E.C.M. type matrices have been made available and are used in many cell culture techniques. We have previously demonstrated that cells cultured on collagen and Matrigel do not differentiate as they do in vivo. It seems that these cultures often produce cells that show a disruption in the secretory process. The appearance of large ribosomal studded vesicles, that specifically label with antibody to casein, suggest an interruption of both protein maturation and secretion at the E.R. to golgi transition. In this report we have examined cultures on collagen and Matrigel at relative high and low seeding densities and compared them to cells from the in vivo condition.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

Automated cell counting of astrocytes on patterned substrates containing aliphatic and charged properties

1995 ◽  
Vol 53 ◽  
pp. 974-975
Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Culture Method ◽  
Cell Counting ◽  
Data Sets ◽  
Nuclear Staining ◽  
Double Positive ◽  
A Cell ◽  
Wafer Test ◽  
Cell Densities

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.

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