scholarly journals A Thermotolerant Marine Bacillus amyloliquefaciens S185 Producing Iturin A5 for Antifungal Activity against Fusarium oxysporum f. sp. cubense

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 516
Author(s):  
Pratiksha Singh ◽  
Jin Xie ◽  
Yanhua Qi ◽  
Qijian Qin ◽  
Cheng Jin ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Bacillus Amyloliquefaciens ◽  
Morphological Changes ◽  
Carbon Sources ◽  
Cost Effective ◽  
Antifungal Compound ◽  
Ionization Mass ◽  
Panama Disease ◽  
Plant Growth Promoting

Fusarium wilt of banana (also known as Panama disease), is a severe fungal disease caused by soil-borne Fusarium oxysporum f. sp. cubense (Foc). In recent years, biocontrol strategies using antifungal microorganisms from various niches and their related bioactive compounds have been used to prevent and control Panama disease. Here, a thermotolerant marine strain S185 was identified as Bacillus amyloliquefaciens, displaying strong antifungal activity against Foc. The strain S185 possesses multiple plant growth-promoting (PGP) and biocontrol utility properties, such as producing indole acetic acid (IAA) and ammonia, assimilating various carbon sources, tolerating pH of 4 to 9, temperature of 20 to 50 °C, and salt stress of 1 to 5%. Inoculation of S185 colonized the banana plants effectively and was mainly located in leaf and root tissues. To further investigate the antifungal components, compounds were extracted, fractionated, and purified. One compound, inhibiting Foc with minimum inhibitory concentrations (MICs) of 25 μg/disk, was identified as iturin A5 by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR). The isolated iturin, A5, resulted in severe morphological changes during spore germination and hyphae growth of Foc. These results specify that B. amyloliquefaciens S185 plays a key role in preventing the Foc pathogen by producing the antifungal compound iturin A5, and possesses potential as a cost-effective and sustainable biocontrol strain for Panama disease in the future. This is the first report of isolation of the antifungal compound iturin A5 from thermotolerant marine B. amyloliquefaciens S185.

scholarly journals Exploration of multitrait antagonistic microbes against Fusarium oxysporum f.sp. lycopersici

2019 ◽  
Vol 11 (2) ◽  
pp. 503-510 ◽  
Author(s):  
Prachi Singh ◽  
Jyoti Singh ◽  
Rahul Singh Rajput ◽  
Anukool Vaishnav ◽  
Shatrupa Ray ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Cost Effective ◽  
Field Studies ◽  
Dual Culture ◽  
Cost Effective Approach ◽  
Maximum Inhibition ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Dual Culture Assay

Fusarium wilt is one of the major diseases of tomato causing extensive loss of production. Exploration of agriculturally important microbes (AIMs) for management of the tomato wilt is an ecofriendly and cost effective approach. In the present study, a total 30 Trichoderma and 30 bacterial isolates were screened in the laboratory for their biocontrol activity against Fusarium oxysporum f.sp. lycopersici (FOL). Out of all the isolates tested, Trichoderma asperellum BHU P-1 and Ochrobactrum sp. BHU PB-1 were found to show maximum inhibition of FOL in dual culture assay. Both the microbes also exhibited plant growth promoting activities such as phosphate solubilisation, production of siderophore, hydrogen cyanide (HCN), indole acetic acid (IAA) and protease activity. These microbes could be evaluated further in greenhouse and field studies for their potential use in management of Fusarium wilt of tomato.

Morphological changes of Fusarium oxysporum induced by CF66I, an antifungal compound from Burkholderia cepacia

2010 ◽  
Vol 32 (10) ◽  
pp. 1487-1495 ◽  
Author(s):  
Xin Li ◽  
Hui-Ying Yu ◽  
Yi-Feng Lin ◽  
Hong-Mei Teng ◽  
Lei Du ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Burkholderia Cepacia ◽  
Morphological Changes ◽  
Antifungal Compound

scholarly journals Draft Genome Sequence of Bacillus amyloliquefaciens Strain RHNK22, Isolated from Rhizosphere with Biosurfactant (Surfactin, Iturin, and Fengycin) and Antifungal Activity

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Papathoti Narendra Kumar ◽  
T. H. Swapna ◽  
Koppula Sathi Reddy ◽  
K. Archana ◽  
Lingampalli Nageshwar ◽  
...  
Keyword(s):  
Surface Tension ◽  
Antifungal Activity ◽  
Plant Growth ◽  
Genome Sequence ◽  
Bacillus Amyloliquefaciens ◽  
Biochemical Analysis ◽  
Draft Genome ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

Bacillus amyloliquefaciens strain RHNK22 isolated from groundnut rhizosphere showed direct and indirect plant growth-promoting traits along with biosurfactant activity and reduction in surface tension of water. Biosurfactants were identified as lipopeptides (surfactin, iturin, and fengycin) by molecular and biochemical analysis in our studies.

scholarly journals Antifungal Activity of Bacillus amyloliquefaciens NJN-6 Volatile Compounds against Fusarium oxysporum f. sp. cubense

2012 ◽  
Vol 78 (16) ◽  
pp. 5942-5944 ◽  
Author(s):  
Jun Yuan ◽  
Waseem Raza ◽  
Qirong Shen ◽  
Qiwei Huang
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Volatile Compounds ◽  
Spore Germination ◽  
Bacillus Amyloliquefaciens ◽  
Fungal Growth ◽  
Content Type

ABSTRACTBacillus amyloliquefaciensNJN-6 produces volatile compounds (VOCs) that inhibit the growth and spore germination ofFusarium oxysporumf. sp.cubense. Among the total of 36 volatile compounds detected, 11 compounds completely inhibited fungal growth. The antifungal activity of these compounds suggested that VOCs can play important roles over short and long distances in the suppression ofFusarium oxysporum.

scholarly journals Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum f. sp. cubense race 1

2021 ◽  
pp. 466-478
Author(s):  
Mileidy Cruz Martín ◽  
Liliana Leyva ◽  
Mayra Acosta Suárez ◽  
Tatiana Pichardo ◽  
Idalmis Bermúdez Caraballoso ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Culture Filtrate ◽  
Bacillus Amyloliquefaciens ◽  
Biological Control Agent ◽  
Antifungal Effect ◽  
Antagonistic Microorganisms ◽  
Race 1

Introduction. Due to the absence of totally effective either economically viable chemical agents for the control of Fusarium wilt, the use of antagonistic microorganisms is of great interest since it could represent a more economically and ecologically sustainable alternative. Objective. To analyze the antifungal effect of the Bacillus amyloliquefaciens CCIBP-A5 strain against Fusarium oxysporum. Materials and methods. The work was carried out in the Laboratory of Applied Microbiology of the Instituto de Biotecnología de las Plantas, Cuba, between September 2017 and June, 2018. The in vitro and in vivo antifungal activity of its culture filtrate and cell against F. oxysporum has been assayed. Results. The results indicated that the metabolites present in the culture filtrate of B. amyloliquefaciens CCIBP-A5 significantly influenced the growth and morphology of the mycelium and the conidia. They also caused oxidative damage to the lipid molecules of F. oxysporum. In addition, this strain showed inhibitory effects on the development of the disease under controlled conditions. These aspects are key when selecting a bacterial candidate as a biological control agent. Conclusions. The results showed that the B. amyloliquefaciens CCIBP-A5 strain, isolated from Musa sp., had an in vitro antifungal effect against the vegetative and reproductive structures of Foc race 1 as well as on the Musa spp.-F. oxysporum interaction. This strain is suggested for the development of a bioproduct for Fusarium wilt management.

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