scholarly journals Comparison of Polypropylene and Ceramic Microfiltration Membranes Applied for Separation of 1,3-PD Fermentation Broths and Saccharomyces cerevisiae Yeast Suspensions

Membranes ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 44
Author(s):  
Wirginia Tomczak ◽  
Marek Gryta
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ceramic Membrane ◽  
Fermentation Broth ◽  
Permeate Flux ◽  
Yeast Saccharomyces Cerevisiae ◽  
Duration Of Action ◽  
Cleaning Agents ◽  
Efficiency Performance ◽  
Microfiltration Membranes ◽  
Fermentation Broths

In recent years, microfiltration (MF) has gained great interest as an excellent technique for clarification of biological suspensions. This paper addresses a direct comparison of efficiency, performance and susceptibility to cleaning of the ceramic and polymeric MF membranes applied for purification of 1,3-propanediol (1,3-PD) fermentation broths and suspensions of yeast Saccharomyces cerevisiae. For this purpose, ceramic, titanium dioxide (TiO2) based membranes and polypropylene (PP) membranes were used. It has been found that both TiO2 and PP membranes provide sterile permeate during filtration of 1,3-PD broths. However, the ceramic membrane, due to the smaller pore diameter, allowed obtaining a better quality permeate. All the membranes used were highly susceptible to fouling with the components of the clarified broths and yeast suspensions. The significant impact of the feed flow velocity and fermentation broth composition on the relative permeate flux has been demonstrated. Suitable cleaning agents with selected concentration and duration of action effectively cleaned the ceramic membrane. In turn, the use of aggressive cleaning solutions led to degradation of the PP membranes matrix. Findings of this study add to a growing body of literature on the use of ceramic and polypropylene MF membranes for the clarification of biological suspensions.

scholarly journals Cross-Flow Microfiltration of Glycerol Fermentation Broths with Citrobacter freundii

Membranes ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 67 ◽  
Author(s):  
Wirginia Tomczak ◽  
Marek Gryta
Keyword(s):  
Colloidal Particles ◽  
Ceramic Membrane ◽  
Fermentation Broth ◽  
Cross Flow ◽  
Transmembrane Pressure ◽  
Citrobacter Freundii ◽  
Permeate Flux ◽  
Glycerol Fermentation ◽  
Fermentation Broths ◽  
The Impact

This paper reports the study of the cross-flow microfiltration (MF) of glycerol fermentation broths with Citrobacter freundii bacteria. A single channel tubular ceramic membrane with a nominal pore size of 0.14 µm was used. It has been demonstrated that the MF ceramic membrane has been successfully applied to bacteria cell removal and to effectively eliminate colloidal particles from glycerol fermentation broths. However, due to fouling, the significant reduction of the MF performance has been demonstrated. In order to investigate the impact of transmembrane pressure (TMP) and feed flow rate (Q) on MF performance, 24 experiments have been performed. The highest steady state permeate flux (138.97 dm3/m2h) was achieved for 0.12 MPa and 1000 dm3/h. Fouling analysis has been studied based on the resistance-in series model. It has been found that the percentage of irreversible fouling resistance during the MF increases with increasing TMP and Q. The permeate flux regeneration has been achieved by membrane cleaning with 3 wt % NaOH and 3 wt % H3PO4 at 45 °C. The results of this study are expected to be useful in industrially employing the MF process as the first step of glycerol fermentation broth purification.

Effect of SnO2 Modification on Permeate Flux of Α-Al2O3 Microfiltration Membrane for Treating Fe(OH)2 Suspensions

2011 ◽  
Vol 189-193 ◽  
pp. 489-493 ◽  
Author(s):  
Xue Bing Hu ◽  
Jian Er Zhou ◽  
Yan Xiang Wang ◽  
Yong Qing Wang ◽  
Xiao Zheng Zhang ◽  
...  
Keyword(s):  
Ceramic Membrane ◽  
Pure Water ◽  
Synthesis Method ◽  
In Situ Synthesis ◽  
Permeate Flux ◽  
Microfiltration Membrane ◽  
Microfiltration Membranes ◽  
Flux Increase ◽  
Nano Grains

To improve the permeate flux of ceramic membrane, commercial α-Al2O3 microfiltration membrane of 0.2 μm pore diameter was modified with SnO2 nano grains by in situ synthesis method. The permeate fluxes of the membrane for pure water and treating Fe(OH)2 suspensions were studied mainly in the paper. The results showed that the pure water stable permeate fluxes of unmodified and SnO2 modified microfiltration membranes were 0.61 m3h-1m-2 and 1.87 m3h-1m-2, respectively. When treating 1L 0.01 mol∙L-1 Fe(OH)2 suspensions, the permeate fluxes of SnO2 modified membrane was higher than that of unmodified membrane all the time and the clarified permeate was obtained. The results indicated that in situ synthesis method can be successfully used to modify α-Al2O3 microfiltration membrane with SnO2 nano grains for permeate flux increase, and the microfiltration membrane has excellent treatment effect on Fe(OH)2 suspensions. Furthermore, the enhancement mechanism of permeate flux of SnO2 modification microfiltration membrane was discussed initially.

PREPARASI DEINOCOCCUS RADIODURANS DAN KHAMIR DALAM MATERIAL KECAP L-DRYING SEBAGAI BAHAN UJI PROFISIENSI

2016 ◽  
Vol 13 (1) ◽  
pp. 93
Author(s):  
Titin Yulinery ◽  
Ratih M.Dewi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Deinococcus Radiodurans ◽  
Test Preparation ◽  
Quality Parameters ◽  
Soy Sauce ◽  
Yeast Saccharomyces Cerevisiae ◽  
Microbiological Test ◽  
Bacterial Contaminants ◽  
Minimum Number ◽  
Important Quality

Tes kemampuan adalah salah satu kegiatan penting dalam pengendalian mutu dan jaminan kualitas mikrobiologi laboratorium untuk mengukur kompetensi analis dan analisis uji profisiensi membutuhkan persiapan Model mikroorganisme adalah kualitas standar dan validitas. Mikrobiologi uji kualitas produk kedelai utama diarahkan pada kehadiran Saccharomyces cerevisiae ragi (S. cerevisiae), S. Bailli, S. rouxii dankontaminan bakteri seperti Bacillus dan Deinococcus. Jenis ragi dan bakteri yang terlibat dalam proses dan dapat menjadi salah satu parameter kualitas penting dalam persiapan yang dihasilkan. Jumlah dan viabilitas bakteri dan ragi menjadi parameter utama dalam proses persiapan bahan uji. Jumlah tersebut adalah jumlah minimum yang berlaku dapat dianalisis. Jumlah ini harus dibawah 10 CFU diperlukan untuk menunjukkan tingkat hygienitas proses dan tingkat minimal kontaminasi. Viabilitas bakteri dan bahan tes ragi persiapan untuk tes kemahiran kecap yang diawetkan dengan L-pengeringan adalah teknik Deinococcus radiodurans (D. radiodurans) 16 tahun, 58 tahun S. cerevisiae, dan S. roxii 13 tahun. kata kunci: Viabilitas, Deinococcus, khamir, L-pengeringan, Proficiency AbstractProficiency test is one of the important activities in quality control and quality assurance microbiology laboratory for measuring the competence of analysts and analysis Proficiency test requires a model microorganism preparations are standardized quality and validity. Microbiological test of the quality of the main soy products aimed at thepresence of yeast Saccharomyces cerevisiae (S. cerevisiae), S. bailli, S. rouxii and bacterial contaminants such as Bacillus and Deinococcus. Types of yeasts and bacteria involved in the process and can be one of the important quality parameters in the preparation produced. The number and viability of bacteria and yeasts become themain parameters in the process of test preparation materials. The amount in question is the minimum number that is valid can be analyzed. This amount must be below 10 CFU required to indicate the level of hygienitas process and the minimum level of contamination. Viability of bacteria and yeast test preparation materials for proficiencytest of soy sauce that preserved by L-drying technique is Deinococcus radiodurans ( D. radiodurans ) 16 years, 58 years S. cerevisiae, and S. roxii 13 years. key words : Viability, Deinococcus, Khamir, L-drying, Proficiency

INTERACTION OF THE HIM1 GENE PRODUCT WITH HELICASES Srs2 (RadH) AND Mph1 YEAST SACCHAROMYCES CEREVISIAE

Tsitologiya ◽  
2018 ◽  
Vol 60 (7) ◽  
pp. 555-557 ◽  
Author(s):  
E. A. Alekseeva ◽  
◽  
T. A. Evstyukhina ◽  
V. T. Peshekhonov ◽  
V. G. Korolev ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Product ◽  
Yeast Saccharomyces Cerevisiae
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