Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by 1H-NMR

In the present study, data related to the metabolomics of saliva and serum in trained standardbred horses are provided for the first time. Metabolomic analysis allows to analyze all the metabolites within selected biofluids, providing a better understanding of biochemistry modifications related to exercise. On the basis of the current advances observed in metabolomic research on human athletes, we aimed to investigate the metabolites’ profile of serum and saliva samples collected from healthy standardbred horses and the relationship with physical exercise. Twelve trained standardbred horses were sampled for blood and saliva before (T0) and immediately after (T1) standardized exercise. Metabolomic analysis of both samples was performed by 1H-NMR spectroscopy. Forty-six metabolites in serum and 62 metabolites in saliva were detected, including alcohols, amino acids, organic acids, carbohydrates and purine derivatives. Twenty-six and 14 metabolites resulted to be significantly changed between T0 and T1 in serum and saliva, respectively. The findings of 2-hydroxyisobutyrate and 3-hydroxybutyrate in serum and GABA in equine saliva, as well as their modifications following exercise, provide new insights about the physiology of exercise in athletic horses. Glycerol might represent a novel biomarker for fitness evaluation in sport horses.

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Comparative Metabolite Profiling of Wild and Cultivated Justicia procumbens L. Based on 1H-NMR Spectroscopy and HPLC-DAD Analysis

Plants ◽

10.3390/plants9070860 ◽

2020 ◽

Vol 9 (7) ◽

pp. 860

Author(s):

Hyunyong Lee ◽

Jihyun Jeon ◽

Joobyoung Yoon ◽

Seung-Hwan Kim ◽

Hyun Sik Choi ◽

...

Keyword(s):

1H Nmr ◽

1H Nmr Spectroscopy ◽

Raw Material ◽

Cultivated Plants ◽

Phase I Clinical Trials ◽

Marker Compound ◽

Nmr Data ◽

Asthma Drug ◽

Phytochemical Constituents ◽

First Time

Justicia procumbens L. is known across Korea, India, China, and Taiwan as a remedy against fever, cough, sore throat, and cirrhosis of ascites. J. procumbens provides the raw material for a candidate anti-asthma drug (DW2008S) currently completing phase I clinical trials sponsored by Dong Wha Pharmaceutical Company. HPLC-DAD was used to quantify phytochemical constituents of J. procumbens, and HPLC and 1H-NMR results were assessed by multivariate analysis. This is the first time a comparative study using HPLC-DAD and NMR fingerprints has been applied to identify chemical differences between wild and cultivated J. procumbens. The amount of justicidin B as the marker compound was higher in cultivated samples (0.80 ± 0.25 mg/g) than in wild ones (0.63 ± 0.30 mg/g). Orthogonal partial least squares discriminant analysis (OPLS-DA) from HPLC and NMR data revealed that there were clear differences between wild and cultivated types and identified five secondary metabolites, which could help distinguish between wild and cultivated plants. Among these five lignans, diphyllin showed the most potent discrimination between two types and was significantly detected higher in cultivated ones than in wild ones. A combination of 1H-NMR and HPLC-DAD analysis is effective for J. procumbens standardization and metabolomics studies.

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Experience affects exercise-induced changes in catecholamines, glucose, and FFA

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1989.256.1.r169 ◽

1989 ◽

Vol 256 (1) ◽

pp. R169-R173 ◽

Cited By ~ 6

Author(s):

A. J. Scheurink ◽

A. B. Steffens ◽

G. H. Dreteler ◽

L. Benthem ◽

R. Bruntink

Keyword(s):

Metabolic Response ◽

Plasma Catecholamines ◽

Plasma Free Fatty Acid ◽

Blood Component ◽

Experimental Conditions ◽

Glucose Levels ◽

Exercise Induced ◽

Induced Changes ◽

Response To Exercise ◽

First Time

The interference of the experimental conditions on the exercise-induced alterations in plasma catecholamines, plasma free fatty acids, and glucose and insulin concentrations was investigated in rats. Exercise consisted of strenuous swimming against a countercurrent (0.22 m/s) for 15 min in a pool with water of 33 degrees C. Before, during, and after swimming, blood samples were taken through a permanent heart catheter. The blood component levels in rats that were confronted with exercise for the very first time were compared with the levels in rats that were well accustomed to the exercise conditions. The very first time rats swam caused an enhanced release of epinephrine from the adrenal medulla and a reduced output of norepinephrine from the sympathetic nerve endings. Furthermore, in the first time swim group, blood glucose levels were higher and plasma free fatty acid concentrations were lower compared with the well-accustomed animals. There were no differences in plasma insulin concentrations. It is concluded that the experimental conditions may interfere considerably with the hormonal and metabolic response to exercise. Furthermore the results reinforce the idea that the two parts of the sympathoadrenal system are functionally and metabolically dissociated.

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1H NMR Spectroscopy and MVA to Evaluate the Effects of Caulerpin-Based Diet on Diplodus sargus Lipid Profiles

Marine Drugs ◽

10.3390/md16100390 ◽

2018 ◽

Vol 16 (10) ◽

pp. 390 ◽

Cited By ~ 8

Author(s):

Laura Del Coco ◽

Serena Felline ◽

Chiara Girelli ◽

Federica Angilè ◽

Laura Magliozzi ◽

...

Keyword(s):

Lipid Metabolism ◽

Nmr Spectroscopy ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

Benthic Communities ◽

Marine Species ◽

Causal Mechanism ◽

Fish Flesh ◽

Diplodus Sargus ◽

First Time

The biological invasion of the green algae Caulerpa cylindracea represents a serious scientific and public issue in the Mediterranean Sea, essentially due to strong modifications both to habitat structure and native benthic communities. Although alterations in health status and changes in flesh quality of some marine species (dietary exposed to C. cylindracea) have been observed, no studies on cause-effect relationships have been carried out. Here, for the first time, through a controlled feeding experiment followed by 1H NMR Spectroscopy and multivariate analysis (PCA, OPLS-DA), we showed that caulerpin taken with diet is directly responsible of changes observed in metabolic profile of fish flesh, including alteration of lipid metabolism, in particular with a reduction of ω3 PUFA content. The potential of caulerpin to directly modulate lipid metabolism opens up new questions about causal mechanism triggered by algal metabolite also in view of a possible exploitation in the nutraceutical/medical field.

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Mesomerization of S4-Symmetric Tetrahedral Chelate Complex [In4(L3)4]: First-Time Monitored by Temperature-Dependent 1H NMR Spectroscopy

European Journal of Inorganic Chemistry ◽

10.1002/ejic.201000295 ◽

2010 ◽

Vol 2010 (19) ◽

pp. 2903-2906 ◽

Cited By ~ 29

Author(s):

Rolf W. Saalfrank ◽

Harald Maid ◽

Andreas Scheurer ◽

Ralph Puchta ◽

Walter Bauer

Keyword(s):

Nmr Spectroscopy ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

Chelate Complex ◽

Temperature Dependent ◽

First Time

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Maternal Perceptions of the Experience of Attempted Labor Induction and Medically Elective Inductions: Analysis of Survey Results from Listening to Mothers in California

10.21203/rs.2.13945/v1 ◽

2019 ◽

Author(s):

Eugene Declercq ◽

Candice Belanoff ◽

Ronald Iverson

Keyword(s):

Medical Records ◽

Medical Intervention ◽

Study Data ◽

Labor Induction ◽

Cesarean Birth ◽

Cesarean Births ◽

Survey Results ◽

Maternal Preference ◽

First Time ◽

The Relationship

Abstract Background The rate of induction of labor in the U.S. has risen from 9.6% in 1990 to 25.7% in 2017, including 31.7% of first-time births. Recent studies that have examined inductions have been small qualitative studies or relied on either medical records or administrative data. This study examines induction from the perspective of those women who experienced it, with a particular focus on the prevalence and predictors of inductions for nonmedical indications, women’s experience of pressure to induce labor and the relationship between the attempt to medically initiate labor and cesarean section. Methods Study data are drawn from the 2,119 women in the Listening to Mothers in California survey who were planning to have a vaginal birth in 2016. Mothers were asked if there had been an attempt to medically initiate labor, if it actually started labor, if they felt pressured to have the induction, if they had a cesarean and the reason for the induction. Reasons for induction were classified as either medically indicated or elective. Results Almost half (47%) of our respondents indicated an attempt was made to medically induce their labor, and 71% of those attempts initiated labor. More than a third of the attempts (37%) were elective. Attempted induction was most strongly associated with giving birth at 41+ weeks (aOR 3.28; 95% C.I. 2.21-4.87). Elective inductions were more likely among multiparous mothers and in pregnancies at 39 or 40 weeks. The perception of being pressured to have labor induced was related to higher levels of education, maternal preference for less medical intervention in birth, having an obstetrician compared to a midwife and gestational ages of 41+ weeks. Cesarean births were no more likely in the case of elective inductions. Cesarean birth was more likely in the case of induction (aOR 1.51; 95% C.I. 1.11-2.07) and especially following a failed attempt at labor induction (aOR 4.50; 95% C.I. 2.93-6.90). Conclusions Clinicians counselling mothers concerning the need for labor induction should be aware of mothers’ perceptions about birth and engage in true shared decision making in order to avoid the maternal perception of being pressured into labor induction.

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Monitoring of dissolution induced changes in film coat composition by 1H NMR spectroscopy and SEM

Journal of Controlled Release ◽

10.1016/j.jconrel.2007.03.004 ◽

2007 ◽

Vol 119 (2) ◽

pp. 190-196 ◽

Cited By ~ 15

Author(s):

Sandra Strübing ◽

Hendrik Metz ◽

Frank Syrowatka ◽

Karsten Mäder

Keyword(s):

Nmr Spectroscopy ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

Induced Changes

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Metabolomic analysis of the consequences of cadmium exposure in Silene cucubalus cell cultures via 1H NMR spectroscopy and chemometrics

Phytochemistry ◽

10.1016/s0031-9422(02)00719-7 ◽

2003 ◽

Vol 62 (6) ◽

pp. 851-858 ◽

Cited By ~ 80

Author(s):

Nigel J.C Bailey ◽

Matjaz Oven ◽

Elaine Holmes ◽

Jeremy K Nicholson ◽

Meinhart H Zenk

Keyword(s):

Nmr Spectroscopy ◽

1H Nmr ◽

Cell Cultures ◽

1H Nmr Spectroscopy ◽

Cadmium Exposure ◽

Metabolomic Analysis

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The Relationship of IL-8 and IL-10 Myokines and Performance in Male Marathon Runners Presenting Exercise-Induced Bronchoconstriction

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17082622 ◽

2020 ◽

Vol 17 (8) ◽

pp. 2622

Author(s):

Juliana de Melo Batista dos Santos ◽

André Luis Lacerda Bachi ◽

Luiz Antonio Luna Junior ◽

Roberta Foster ◽

Ana Paula Renno Sierra ◽

...

Keyword(s):

Negative Impact ◽

Marathon Runners ◽

Tnf Α ◽

Lower Maximum ◽

Maximum Aerobic Capacity ◽

And Performance ◽

Exercise Induced ◽

Relationship Of ◽

First Time ◽

The Relationship

At present, it is unclear which exercise-induced factors, such as myokines, could diminish the negative impact of the reduction in pulmonary function imposed by the exercise in question. In this study, we aim to evaluate the prevalence of exercise-induced bronchoconstriction (EIB) and also to investigate the effect of myokines in the performance of marathon runners presenting EIB or not. Thirty-eight male recreational marathon runners (age 38.8 [33–44], height 175.7 [172.0–180.3]; weight 74.7 [69.3–81.6]) participated in this study, and through spirometry tests, a prevalence of 23.6% of EIB was found, which is in agreement with the literature. The volunteers who tested positive to EIB (EIB+) presented lower maximum aerobic capacity compared to those who tested negative (EIB−) (EIB+ 44.02 [39.56–47.02] and EIB− 47.62 [44.11–51.18] p = 0.03). The comparison of plasma levels of IL-1β (EIB+ p = 0.296, EIB− p = 0.176, EIB+ vs. EIB− baseline p = 0.190 immediately after p = 0.106), IL-4 (undetectable), IL-6 (EIB+ p = 0.003, EIB− p ≤ 0.001, EIB+ vs. EIB− baseline p = 0.301 immediately after p = 0.614), IL-8 (EIB+ p = 0.003, EIB− p ≤ 0.001, EIB+ vs. EIB− baseline p = 0.110 immediately after p = 0.453), IL-10 (EIB+ p = 0.003, EIB− p ≤ 0.001, EIB+ vs. EIB− baseline p = 0.424 immediately after p = 0.876) and TNF-α (EIB+ p = 0.003, EIB− p ≤ 0.001, EIB+ vs. EIB− baseline p = 0.141 immediately after p = 0.898) were similar in both groups 24 h before and immediately after the marathon. However, negative correlations were found between the marathon finishing time and the levels of IL-8 (r = −0.81, p = 0.022), and IL-10 (r = −0.97, p ≤ 0.001) immediately after completing the marathon. In conclusion, for the first time, it is shown that the myokines IL-8 and IL-10 are related to improvement of the performance of marathon runners presenting EIB.

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PSYCHE—A Valuable Experiment in Plant NMR-Metabolomics

Molecules ◽

10.3390/molecules25215125 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5125

Author(s):

Pauline Stark ◽

Caroline Zab ◽

Andrea Porzel ◽

Katrin Franke ◽

Paride Rizzo ◽

...

Keyword(s):

1H Nmr ◽

Multivariate Data Analysis ◽

Pulse Sequence ◽

Spectroscopic Method ◽

Evaluation Criteria ◽

Metabolomic Analysis ◽

High Complexity ◽

Signal Overlap ◽

The Relationship ◽

Hypericum Species

1H-NMR is a very reproducible spectroscopic method and, therefore, a powerful tool for the metabolomic analysis of biological samples. However, due to the high complexity of natural samples, such as plant extracts, the evaluation of spectra is difficult because of signal overlap. The new NMR “Pure Shift” methods improve spectral resolution by suppressing homonuclear coupling and turning multiplets into singlets. The PSYCHE (Pure Shift yielded by Chirp excitation) and the Zangger–Sterk pulse sequence were tested. The parameters of the more suitable PSYCHE experiment were optimized, and the extracts of 21 Hypericum species were measured. Different evaluation criteria were used to compare the suitability of the PSYCHE experiment with conventional 1H-NMR. The relationship between the integral of a signal and the related bin value established by linear regression demonstrates an equal representation of the integrals in binned PSYCHE spectra compared to conventional 1H-NMR. Using multivariate data analysis based on both techniques reveals comparable results. The obtained data demonstrate that Pure Shift spectra can support the evaluation of conventional 1H-NMR experiments.

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