scholarly journals Metabolomic Analysis of Response to Nitrogen-Limiting Conditions in Yarrowia spp.

Metabolites ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 16
Author(s):  
Sivamoke Dissook ◽  
Sastia Prama Putri ◽  
Eiichiro Fukusaki
Keyword(s):  
Time Course ◽  
Industrial Applications ◽  
Metabolomic Analysis ◽  
Metabolomic Profile ◽  
Metabolome Analysis ◽  
Yeast Genus ◽  
Factors Affecting ◽  
Carbon To Nitrogen Ratio ◽  
Cultivation Time ◽  
Nitrogen Ratio

Yarrowia is a yeast genus that has been used as a model oleaginous taxon for a wide array of studies. However, information regarding metabolite changes within Yarrowia spp. under different environmental conditions is still limited. Among various factors affecting Yarrowia metabolism, nitrogen-limiting conditions have a profound effect on the metabolic state of yeast. In this study, a time-course LC-MS/MS-based metabolome analysis of Y. lipolytica was performed to determine the optimal cultivation time and carbon-to-nitrogen ratio for studying the effects of nitrogen-limiting conditions on Yarrowia; we found that cultivation time of 36 h and carbon-to-nitrogen ratio of 4:1 and 5:0 was suitable for studying the effects of nitrogen-limiting conditions on Yarrowia and these conditions were applied to six strains of Yarrowia. These six strains of Yarrowia showed similar responses to nitrogen-limiting conditions; however, each strain had a unique metabolomic profile. Purine and pyrimidine metabolism were the most highly affected biological pathways in nitrogen-limiting conditions, indicating that these conditions affect energy availability within cells. This stress leads to a shift in cells to the utilization of a less ATP-dependent biological pathway. This information will be beneficial for the development of Yarrowia strains for further scientific and industrial applications.

Preliminary comparative deep metabolomic analysis of spermatozoa from zebu and crossbred cattle suggests associations between metabolites, sperm quality and fertility

2021 ◽  
Vol 33 (6) ◽  
pp. 427
Author(s):  
Mohua DasGupta ◽  
Arumugam Kumaresan ◽  
Kaustubh Kishor Saraf ◽  
Gayathree Karthikkeyan ◽  
T. S. Keshava Prasad ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Semen Quality ◽  
Sperm Quality ◽  
Tandem Mass ◽  
Metabolomic Analysis ◽  
Metabolomic Profile ◽  
Crossbred Cattle ◽  
Acetyl Coenzyme A ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Poor semen quality and infertility/subfertility are more frequent in crossbred than zebu bulls. Using a high-throughput liquid chromatography–tandem mass spectrometry (LC-MS/MS)-based approach, we established the preliminary metabolomic profile of crossbred and zebu bull spermatozoa (n=3 bulls each) and identified changes in sperm metabolomics between the two groups. In all, 1732 and 1240 metabolites were detected in zebu and crossbred bull spermatozoa respectively. After excluding exogenous metabolites, 115 and 87 metabolites were found to be unique to zebu and crossbred bull spermatozoa respectively whereas 71 metabolites were common to both. In the normalised data, 49 metabolites were found to be differentially expressed between zebu and crossbred bull spermatozoa. The significantly enriched (P<0.05) pathways in spermatozoa were taurine and hypotaurine metabolism (observed metabolites taurine and hypotaurine) in zebu and glycerophospholipid metabolism (observed metabolites phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine) in crossbred bulls. The abundance of nitroprusside (variable importance in projection (VIP) score >1.5) was downregulated, whereas that of l-cysteine, acetyl coenzyme A and 2′-deoxyribonucleoside 5′-diphosphate (VIP scores >1.0) was upregulated in crossbred bull spermatozoa. In conclusion, this study established the metabolomic profile of zebu and crossbred bull spermatozoa and suggests that aberrations in taurine, hypotaurine and glycerophospholipid metabolism may be associated with the higher incidence of infertility/subfertility in crossbred bulls.

scholarly journals Factors affecting the appearance of the hump charge movement component in frog cut twitch fibers.

1991 ◽  
Vol 98 (2) ◽  
pp. 315-347 ◽  
Author(s):  
C S Hui
Keyword(s):  
Time Course ◽  
Extreme Case ◽  
Complete Recovery ◽  
Creatine Phosphate ◽  
Boltzmann Distribution ◽  
Distribution Functions ◽  
Charge Movement ◽  
Factors Affecting ◽  
Gap Technique ◽  
Voltage Dependent

Charge movement was measured in frog cut twitch fibers with the double Vaseline gap technique. Five manipulations listed below were applied to investigate their effects on the hump component (I gamma) in the ON segments of TEST minus CONTROL current traces. When external Cl-1 was replaced by MeSO3- to eliminate Cl current, I gamma peaked earlier due to a few millivolts shift of the voltage dependence of I gamma kinetics in the negative direction. The Q-V plots in the TEA.Cl and TEA.MeSO3 solutions were well fitted by a sum of two Boltzmann distribution functions. The more steeply voltage-dependent component (Q gamma) had a V approximately 6 mV more negative in the TEA.MeSO3 solution than in the TEA.Cl solution. These voltage shifts were partially reversible. When creatine phosphate in the end pool solution was removed, the I gamma hump disappeared slowly over the course of 20-30 min, partly due to a suppression of Q gamma. The hump reappeared when creatine phosphate was restored. When 0.2-1.0 mM Cd2+ was added to the center pool solution to block inward Ca current, the I gamma hump became less prominent due to a prolongation in the time course of I gamma but not to a suppression of Q gamma. When the holding potential was changed from -90 to -120 mV, the amplitude of I beta was increased, thereby obscuring the I gamma hump. Finally, when a cut fiber was stimulated repetitively, I gamma lost its hump appearance because its time course was prolonged. In an extreme case, a 5-min resting interval was insufficient for a complete recovery of the waveform. In general, a stimulation rate of once per minute had a negligible effect on the shape of I gamma. Of the five manipulations, MeSO3- has the least perturbation on the appearance of I gamma and is potentially a better substitute for Cl- than SO2-(4) in eliminating Cl current if the appearance of the I gamma hump is to be preserved.

scholarly journals Association of ectomycorrhizal trees with high carbon-to-nitrogen ratio soils across temperate forests is driven by smaller nitrogen not larger carbon stocks

2018 ◽  
Vol 106 (2) ◽  
pp. 524-535 ◽  
Author(s):  
Kai Zhu ◽  
M. Luke McCormack ◽  
Richard A. Lankau ◽  
J. Franklin Egan ◽  
Nina Wurzburger
Keyword(s):  
Temperate Forests ◽  
Carbon Stocks ◽  
High Carbon ◽  
Carbon To Nitrogen Ratio ◽  
Nitrogen Ratio

scholarly journals Factors controlling nitrous oxide emissions from managed northern peat soils with low carbon to nitrogen ratio

2018 ◽  
Vol 122 ◽  
pp. 186-195 ◽  
Author(s):  
Maarit Liimatainen ◽  
Carolina Voigt ◽  
Pertti J. Martikainen ◽  
Jyrki Hytönen ◽  
Kristiina Regina ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Nitrous Oxide Emissions ◽  
Low Carbon ◽  
Peat Soils ◽  
Carbon To Nitrogen Ratio ◽  
Nitrogen Ratio
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