scholarly journals Antibiotic-Induced Dysbiosis of Microbiota Promotes Chicken Lipogenesis by Altering Metabolomics in the Cecum

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 487
Author(s):  
Tao Zhang ◽  
Hao Ding ◽  
Lan Chen ◽  
Yueyue Lin ◽  
Yongshuang Gong ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
High Performance ◽  
Fat Deposition ◽  
16S Rrna Sequencing ◽  
Oral Antibiotics ◽  
Ms Analysis ◽  
Rrna Sequencing ◽  
Cecal Microbiota ◽  
Metabolite Network

Elucidation of the mechanism of lipogenesis and fat deposition is essential for controlling excessive fat deposition in chicken. Studies have shown that gut microbiota plays an important role in regulating host lipogenesis and lipid metabolism. However, the function of gut microbiota in the lipogenesis of chicken and their relevant mechanisms are poorly understood. In the present study, the gut microbiota of chicken was depleted by oral antibiotics. Changes in cecal microbiota and metabolomics were detected by 16S rRNA sequencing and ultra-high performance liquid chromatography coupled with MS/MS (UHPLC–MS/MS) analysis. The correlation between antibiotic-induced dysbiosis of gut microbiota and metabolites and lipogenesis were analysed. We found that oral antibiotics significantly promoted the lipogenesis of chicken. 16S rRNA sequencing indicated that oral antibiotics significantly reduced the diversity and richness and caused dysbiosis of gut microbiota. Specifically, the abundance of Proteobacteria was increased considerably while the abundances of Bacteroidetes and Firmicutes were significantly decreased. At the genus level, the abundances of genera Escherichia-Shigella and Klebsiella were significantly increased while the abundances of 12 genera were significantly decreased, including Bacteroides. UHPLC-MS/MS analysis showed that antibiotic-induced dysbiosis of gut microbiota significantly altered cecal metabolomics and caused declines in abundance of 799 metabolites and increases in abundance of 945 metabolites. Microbiota-metabolite network revealed significant correlations between 4 differential phyla and 244 differential metabolites as well as 15 differential genera and 304 differential metabolites. Three metabolites of l-glutamic acid, pantothenate acid and N-acetyl-l-aspartic acid were identified as potential metabolites that link gut microbiota and lipogenesis in chicken. In conclusion, our results showed that antibiotic-induced dysbiosis of gut microbiota promotes lipogenesis of chicken by altering relevant metabolomics. The efforts in this study laid a basis for further study of the mechanisms that gut microbiota regulates lipogenesis and fat deposition of chicken.

scholarly journals The Pervasive Effects of an Antibiotic on the Human Gut Microbiota, as Revealed by Deep 16S rRNA Sequencing

PLoS Biology ◽  
2008 ◽  
Vol 6 (11) ◽  
pp. e280 ◽  
Author(s):  
Les Dethlefsen ◽  
Sue Huse ◽  
Mitchell L Sogin ◽  
David A Relman
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
16S Rrna Sequencing ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Rrna Sequencing

scholarly journals Characterization of Gut Microbiota in Prenatal Cold Stress Offspring Rats by 16S rRNA Sequencing

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1619
Author(s):  
Jiasan Zheng ◽  
Tingting Zhu ◽  
Lipeng Wang ◽  
Jianfa Wang ◽  
Shuai Lian
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
Cold Stress ◽  
Female Offspring ◽  
16S Rrna Sequencing ◽  
Negative Effects ◽  
Rrna Sequencing ◽  
Temperature Group ◽  
Microbial Dna

Our previous study showed a reduction of anxiety-like behavior in offspring rats suffered from prenatal cold stress; whether this was related to changes in the offspring gut microbiota is unclear. To obtain the evidence for the role of the gut microbiota in prenatal cold stress offspring, 16S rRNA sequencing technology was used. Male and female offspring rat feces were collected from a room temperature group and a prenatal cold stress group (n ≥ 8) for microbial DNA extraction, followed by 16S rRNA sequencing. The results indicated that prenatal cold stress could change the offspring’s gut microbiota composition. Prenatal cold stress significantly upregulates Lactobacillus, Lactobacillus_gasseri, Bacteroides, and Bacteroides-acidifaciens in female offspring, whereas prenatal cold stress significantly reduced Lachnospiraceae and Prevotellaceae in male offspring. These data showed the characterization of gut microbiota in prenatal cold stress offspring rats, and these data suggest that microbiological intervention in the future can potentially prevent the negative effects caused by cold stress to animals.

scholarly journals Cecal microbiota of Tibetan Chickens from five geographic regions were determined by 16S rRNA sequencing

2016 ◽  
Vol 5 (5) ◽  
pp. 753-762 ◽  
Author(s):  
Xueyan Zhou ◽  
Xiaosong Jiang ◽  
Chaowu Yang ◽  
Bingcun Ma ◽  
Changwei Lei ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing ◽  
Geographic Regions ◽  
Cecal Microbiota

Evaluation of the nutrition and function of cow and goat milk based on intestinal microbiota by metagenomic analysis

2018 ◽  
Vol 9 (4) ◽  
pp. 2320-2327 ◽  
Author(s):  
Zhaoxia Wang ◽  
Shuaiming Jiang ◽  
Chenchen Ma ◽  
Dongxue Huo ◽  
Qiannan Peng ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
High Throughput ◽  
Goat Milk ◽  
16S Rrna Sequencing ◽  
Metagenomic Analysis ◽  
Sequencing Technology ◽  
Rrna Sequencing ◽  
And Function

A high-throughput 16S rRNA sequencing technology was applied to study changes of the intestinal microbiota in mice after the administration of cow and goat milk. We show a correlation between the gut microbiota and the nutrients in milk.

scholarly journals Gut Microbiota as Prognosis Markers for Patients with HBV-Related Acute-on-Chronic Liver Failure

2020 ◽  
Author(s):  
Ke Wang ◽  
Zhao Zhang ◽  
Zhi-Shuo Mo ◽  
Xiao-Hua Yang ◽  
Bing-Liang Lin ◽  
...  
Keyword(s):  
Hepatitis B ◽  
16S Rrna ◽  
Gut Microbiota ◽  
Liver Failure ◽  
Chronic Liver ◽  
16S Rrna Sequencing ◽  
Metagenomic Sequencing ◽  
Healthy Individuals ◽  
Chronic Liver Failure ◽  
Rrna Sequencing

Abstract Background The gut microbiota in the hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) is poorly defined. We aim to uncover the characteristics of the gut microbiota in HBV-ACLF and in other HBV associated pathologies. Methods We analyzed the gut microbiome in patients with HBV-ACLF or other HBV associated pathologies and healthy individuals by 16S rRNA sequencing and metagenomic sequencing of fecal samples. 212 patients with HBV-ACLF, 252 with chronic hepatitis B (CHB), 162 with HBV-associated cirrhosis (HBV-LC) and 877 healthy individuals were recruited for the study. CHB and HBV-LC patients are grouped as HBV-Other. Results We discovered striking differences in the microbiome diversity between the HBV-ACLF, HBV-Other and healthy groups using 16S rRNA sequencing. The ratio of cocci to bacilli was significantly elevated in the HBV-ACLF group compared with healthy group. Further analysis within the HBV-ACLF group identified 52 genera showing distinct richness within the group where Enterococcus was enriched in the progression group whilst Faecalibacterium was enriched in the regression group. Metagenomic sequencing validated these findings and further uncovered an enrichment of Lactobacillus casei paracasei in progression group, while Alistipes senegalensis, Faecalibacterium prausnitzii and Parabacteroides merdae dominated the regression group. Importantly, our analysis revealed that there was a rapid increase of Enterococcus faecium during the progression of HBV-ACLF. Conclusions The gut microbiota displayed distinct composition at different phases of HBV-ACLF. High abundance of Enterococcus is associated with progression while that of Faecalibacterium is associated with regression of HBV-ACLF. Therefore the microbiota features hold promising potential as prognostic markers for HBV-ACLF.

scholarly journals Gut microbiota modification suppresses the development of pulmonary arterial hypertension in an SU5416/hypoxia rat model

2020 ◽  
Vol 10 (3) ◽  
pp. 204589402092914
Author(s):  
Takayuki J. Sanada ◽  
Koji Hosomi ◽  
Hiroki Shoji ◽  
Jonguk Park ◽  
Akira Naito ◽  
...  
Keyword(s):  
Pulmonary Arterial Hypertension ◽  
16S Rrna ◽  
Gut Microbiota ◽  
Arterial Hypertension ◽  
Right Ventricular ◽  
Rat Model ◽  
Gut Microbiome ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing ◽  
Pulmonary Arterial

The pathogenesis of pulmonary arterial hypertension is closely associated with dysregulated inflammation. Recently, abnormal alterations in gut microbiome composition and function were reported in a pulmonary arterial hypertension experimental animal model. However, it remains unclear whether these alterations are a result or the cause of pulmonary arterial hypertension. The purpose of this study was to investigate whether alterations in the gut microbiome affected the hemodynamics in SU5416/hypoxia rats. We used the SU5416/hypoxia rat model in our study. SU5416/hypoxia rats were treated with a single SU5416 injection (30 mg/kg) and a three-week hypoxia exposure (10% O2). Three SU5416/hypoxia rats were treated with a combination of four antibiotics (SU5416/hypoxia + ABx group) for four weeks. Another group was exposed to hypoxia (10% O2) without the SU5416 treatment, and control rats received no treatment. Fecal samples were collected from each animal, and the gut microbiota composition was analyzed by 16S rRNA sequencing. The antibiotic treatment significantly suppressed the vascular remodeling, right ventricular hypertrophy, and increase in the right ventricular systolic pressure in SU5416/hypoxia rats. 16S rRNA sequencing analysis revealed gut microbiota modification in SU5416/hypoxia + ABx group. The Firmicutes-to-Bacteroidetes ratio in SU5416/hypoxia rats was significantly higher than that in control and hypoxia rats. Compared with the control microbiota, 14 bacterial genera, including Bacteroides and Akkermansia, increased, whereas seven bacteria, including Rothia and Prevotellaceae, decreased in abundance in SU5416/hypoxia rats. Antibiotic-induced modification of the gut microbiota suppresses the development of pulmonary arterial hypertension. Dysbiosis may play a causal role in the development and progression of pulmonary arterial hypertension.

scholarly journals Associations of Probiotic Fermented Milk (PFM) and Yogurt Consumption with Bifidobacterium and Lactobacillus Components of the Gut Microbiota in Healthy Adults

Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 651 ◽  
Author(s):  
Noemí Redondo-Useros ◽  
Alina Gheorghe ◽  
Ligia Díaz-Prieto ◽  
Brenda Villavisencio ◽  
Ascensión Marcos ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
Streptococcus Thermophilus ◽  
Fermented Milk ◽  
Healthy Adults ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing

The current study investigates whether probiotic fermented milk (PFM) and yogurt consumption (YC) are related to both the ingested bacteria taxa and the overall gut microbiota (GM) composition in healthy adults. PFM and YC habits were analyzed in 260 subjects (51% male) by specific questionnaires, and the following groups were considered: (1) PFM groups: nonconsumers (PFM-NC, n = 175) and consumers (PFM, n = 85), divided as follows: Bifidobacterium-containing PFM (Bif-PFM; n = 33), Lactobacillus-containing PFM (Lb-PFM; n = 14), and mixed Bifidobacterium and Lactobacillus-containing PFM (Mixed-PFM; n = 38); (2) PFM-NC were classified as: yogurt nonconsumers (Y-NC; n = 40) and yogurt consumers (n = 135). GM was analyzed through 16S rRNA sequencing. PFM consumers showed higher Bifidobacteria taxa levels compared to NC, from phylum through to species. Specifically, Bif-PFM consumption was related to higher B. animalis levels (p < 0.001), whereas Lb-PFM consumption was associated to higher levels of Bifidobacterium (p < 0.045) and B. longum (p = 0.011). YC was related to higher levels of the yogurt starter Streptococcus thermophilus (p < 0.001). Lactobacilli and the overall GM were not related either to YC or PFM consumption. According to these results, healthy adults might benefit from PFM intake by increasing Bifidobacterium levels.

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