scholarly journals Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery

2020 ◽  
Vol 8 (12) ◽  
pp. 2029
Author(s):  
Christian C. Blesken ◽  
Tessa Strümpfler ◽  
Till Tiso ◽  
Lars M. Blank
Keyword(s):  
Scale Up ◽  
Recovery Process ◽  
Downstream Processing ◽  
Biosurfactant Production ◽  
Foam Fractionation ◽  
Pseudomonas Putida Kt2440 ◽  
Culture Volume ◽  
Product Removal ◽  
Fractionation Column ◽  
Space Time Yield

The production of biosurfactants is often hampered by excessive foaming in the bioreactor, impacting system scale-up and downstream processing. Foam fractionation was proposed to tackle this challenge by combining in situ product removal with a pre-purification step. In previous studies, foam fractionation was coupled to bioreactor operation, hence it was operated at suboptimal parameters. Here, we use an external fractionation column to decouple biosurfactant production from foam fractionation, enabling continuous surfactant separation, which is especially suited for system scale-up. As a subsequent product recovery step, continuous foam adsorption was integrated into the process. The configuration is evaluated for rhamnolipid (RL) or 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA, i.e., RL precursor) production by recombinant non-pathogenic Pseudomonas putida KT2440. Surfactant concentrations of 7.5 gRL/L and 2.0 gHAA/L were obtained in the fractionated foam. 4.7 g RLs and 2.8 g HAAs could be separated in the 2-stage recovery process within 36 h from a 2 L culture volume. With a culture volume scale-up to 9 L, 16 g RLs were adsorbed, and the space-time yield (STY) increased by 31% to 0.21 gRL/L·h. We demonstrate a well-performing process design for biosurfactant production and recovery as a contribution to a vital bioeconomy.

scholarly journals The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

2021 ◽  
Vol 12 ◽  
Author(s):  
Sizwe I. Mhlongo ◽  
Obinna T. Ezeokoli ◽  
Ashira Roopnarain ◽  
Busiswa Ndaba ◽  
Patrick T. Sekoai ◽  
...  
Keyword(s):  
Single Cell ◽  
Filamentous Fungi ◽  
Process Integration ◽  
Scale Up ◽  
Recovery Process ◽  
Downstream Processing ◽  
Production Costs ◽  
Biodiesel Production ◽  
Industrial Processing ◽  
Single Cell Oils

Microbial lipids, also known as single-cell oils (SCOs), are highly attractive feedstocks for biodiesel production due to their fast production rates, minimal labor requirements, independence from seasonal and climatic changes, and ease of scale-up for industrial processing. Among the SCO producers, the less explored filamentous fungi (molds) exhibit desirable features such as a repertoire of hydrolyzing enzymes and a unique pellet morphology that facilitates downstream harvesting. Although several oleaginous filamentous fungi have been identified and explored for SCO production, high production costs and technical difficulties still make the process less attractive compared to conventional lipid sources for biodiesel production. This review aims to highlight the ability of filamentous fungi to hydrolyze various organic wastes for SCO production and explore current strategies to enhance the efficiency and cost-effectiveness of the SCO production and recovery process. The review also highlights the mechanisms and components governing lipogenic pathways, which can inform the rational designs of processing conditions and metabolic engineering efforts for increasing the quality and accumulation of lipids in filamentous fungi. Furthermore, we describe other process integration strategies such as the co-production with hydrogen using advanced fermentation processes as a step toward a biorefinery process. These innovative approaches allow for integrating upstream and downstream processing units, thus resulting in an efficient and cost-effective method of simultaneous SCO production and utilization for biodiesel production.

scholarly journals Recent Findings in Azaphilone Pigments

2021 ◽  
Vol 7 (7) ◽  
pp. 541
Author(s):  
Lúcia P. S. Pimenta ◽  
Dhionne C. Gomes ◽  
Patrícia G. Cardoso ◽  
Jacqueline A. Takahashi
Keyword(s):  
Water Solubility ◽  
Biological Activities ◽  
Low Cost ◽  
Scale Up ◽  
Structural Diversity ◽  
Downstream Processing ◽  
Global Market ◽  
Yield Improvement ◽  
Potential Applications ◽  
New Compounds

Filamentous fungi are known to biosynthesize an extraordinary range of azaphilones pigments with structural diversity and advantages over vegetal-derived colored natural products such agile and simple cultivation in the lab, acceptance of low-cost substrates, speed yield improvement, and ease of downstream processing. Modern genetic engineering allows industrial production, providing pigments with higher thermostability, water-solubility, and promising bioactivities combined with ecological functions. This review, covering the literature from 2020 onwards, focuses on the state-of-the-art of azaphilone dyes, the global market scenario, new compounds isolated in the period with respective biological activities, and biosynthetic pathways. Furthermore, we discussed the innovations of azaphilone cultivation and extraction techniques, as well as in yield improvement and scale-up. Potential applications in the food, cosmetic, pharmaceutical, and textile industries were also explored.

scholarly journals Influence of toluene and salinity on biosurfactant production by Bacillus sp.: scale up from flasks to a bench-scale bioreactor

2017 ◽  
Vol 34 (2) ◽  
pp. 395-405 ◽  
Author(s):  
Ellen Cristina Souza ◽  
Thereza Christina Vessoni-Penna ◽  
Saleh Al Arni ◽  
José Manuel Domínguez ◽  
Attilio Converti ◽  
...  
Keyword(s):  
Scale Up ◽  
Biosurfactant Production ◽  
Bacillus Sp ◽  
Bench Scale

Kinetics of Chromium Ion Removal from Wastewater with Internal Circulation Foam Flotation

2013 ◽  
Vol 742 ◽  
pp. 384-387 ◽  
Author(s):  
De Jin Wang ◽  
Na Lin
Keyword(s):  
Chemical Reaction ◽  
Active Agent ◽  
Foam Fractionation ◽  
Foam Separation ◽  
Ion Removal ◽  
Fractionation Column ◽  
Foam Flotation ◽  
Reaction Constant ◽  
New Type ◽  
Reaction Processes

A new type of foam fractionation column with spiral internal had been designed for enhancing the foam drainage and thus for the removal of minute hazardous materials. Chromium was separated from wastewater using the continuous foam separation method. According to the similar physical behavior of foam separation and chemical reaction processes, the equivalent chemical reaction constant was introduced, Establishment concentrate distributed mathematical model of the whole tower under the condition of continuous foam separation with SDS as active agent. The model has been tested by experiments. The result indicated that foam separation process could be regarded as a first order reaction, and diffusion coefficient was 4.86cm2/s.

scholarly journals A Feasibility Study of Cellulosic Isobutanol Production—Process Simulation and Economic Analysis

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 667 ◽  
Author(s):  
Avraam Roussos ◽  
Nikiforos Misailidis ◽  
Alexandros Koulouris ◽  
Francesco Zimbardi ◽  
Demetri Petrides
Keyword(s):  
Energy Demand ◽  
Production Cost ◽  
Fossil Fuels ◽  
Raw Materials ◽  
Downstream Processing ◽  
Economic Feasibility ◽  
Unit Production Cost ◽  
Product Titer ◽  
Product Removal ◽  
The Cost

Renewable liquid biofuels for transportation have recently attracted enormous global attention due to their potential to provide a sustainable alternative to fossil fuels. In recent years, the attention has shifted from first-generation bioethanol to the production of higher molecular weight alcohols, such as biobutanol, from cellulosic feedstocks. The economic feasibility of such processes depends on several parameters such as the cost of raw materials, the fermentation performance and the energy demand for the pretreatment of biomass and downstream processing. In this work, two conceptual process scenarios for isobutanol production, one with and one without integrated product removal from the fermentor by vacuum stripping, were developed and evaluated using SuperPro Designer®. In agreement with previous publications, it was concluded that the fermentation titer is a crucial parameter for the economic competitiveness of the process as it is closely related to the energy requirements for product purification. In the first scenario where the product titer was 22 g/L, the energy demand for downstream processing was 15.8 MJ/L isobutanol and the unit production cost of isobutanol was $2.24/L. The integrated product removal by vacuum stripping implemented in the second scenario was assumed to improve the isobutanol titer to 50 g/L. In this case, the energy demand for the product removal (electricity) and downstream processing were 1.8 MJ/L isobutanol and 10 MJ/L isobutanol, respectively, and the unit production cost was reduced to $1.42/L. The uncertainty associated with the choice of modeling and economic parameters was investigated by Monte Carlo simulation sensitivity analysis.

scholarly journals Theoretical analysis of the performance of a foam fractionation column

2014 ◽  
Vol 470 (2165) ◽  
pp. 20130625 ◽  
Author(s):  
S. T. Tobin ◽  
D. Weaire ◽  
S. Hutzler
Keyword(s):  
Theoretical Analysis ◽  
Surfactant Solution ◽  
Model System ◽  
Foam Fractionation ◽  
Fractionation Column ◽  
Foam Drainage Equation ◽  
Limiting Case ◽  
Alternative Set ◽  
Theory And Experiment ◽  
Foam Drainage

A model system for theory and experiment which is relevant to foam fractionation consists of a column of foam moving through an inverted U-tube between two pools of surfactant solution. The foam drainage equation is used for a detailed theoretical analysis of this process. In a previous paper, we focused on the case where the lengths of the two legs are large. In this work, we examine the approach to the limiting case (i.e. the effects of finite leg lengths) and how it affects the performance of the fractionation column. We also briefly discuss some alternative set-ups that are of interest in industry and experiment, with numerical and analytical results to support them. Our analysis is shown to be generally applicable to a range of fractionation columns.

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