scholarly journals Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars

Molecules ◽  
2017 ◽  
Vol 22 (7) ◽  
pp. 1055 ◽  
Author(s):  
You-Ran Jang ◽  
Hye-Rang Beom ◽  
Susan Altenbach ◽  
Min-Ki Lee ◽  
Sun-Hyung Lim ◽  
...  
Keyword(s):  
Common Wheat ◽  
Wheat Cultivars ◽  
Improved Method ◽  
Rp Hplc ◽  
Sds Page

scholarly journals Genetic diversity of Polish cultivars of common wheat (Triticum aestivum L.) based on molecular and protein markers

2020 ◽  
Vol 48 (2) ◽  
pp. 535-548
Author(s):  
Ewa FILIP ◽  
Aleksandra STROŃSKA ◽  
Magdalena SZENEJKO ◽  
Waldemar PLUTA
Keyword(s):  
Genetic Diversity ◽  
Common Wheat ◽  
Similarity Index ◽  
Principal Component ◽  
Shannon Index ◽  
Qualitative Assessment ◽  
Wheat Cultivars ◽  
Protein Markers ◽  
Binary Matrix ◽  
Sds Page

Knowledge of the composition subunits of proteins glutenins makes it much easier to determine the quality and suitability of the final product obtained from gluten. It is important to remember that not only molecular processes model gluten protein polymorphism. In this study, the genetic diversity of Polish common wheat cultivars was examined at the level of DNA and glutenin’s proteins HMW-GS. The SDS-PAGE and RAPD bands were evaluated in binary matrix, which was the basis for further analysis of results, using appropriate measures of variability: Q-Cochran  test (Cochran, 1950), p < 0.05; I-Shannon index; Si-Similarity index; PIC-Polymorphism Information Content; (PCA-Principal component analysis). Both types of markers proved to be useful in the overall assessment of genetic variability between tested of common wheat cultivars. The general genetic diversity indicates that good candidates with unique composition of HMW-GS subunits were selected among the examined cultivars and three OPA-02, OPA-03, OPB-08 primers with the highest power differentiating for the studied genotypes were selected. Results of the research revealed the potentials of RAPD and SDS-PAGE technique in determining genetical diversity and make a suitable qualitative assessment of common wheat cultivars.

scholarly journals Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)

2011 ◽  
Vol 49 (No. 3) ◽  
pp. 99-105 ◽  
Author(s):  
V. Dvořáček ◽  
V. Čurn
Keyword(s):  
Common Wheat ◽  
Biochemical Markers ◽  
Digital Image Analysis ◽  
Wheat Cultivar ◽  
Protein Fractions ◽  
Wheat Cultivars ◽  
Triticum Spelta ◽  
Spelt Wheat ◽  
Sds Page ◽  
Polymorphism Level

Four protein fractions: 1 &ndash; albumins and globulins, 2 &ndash; gliadins, 3 &ndash; glutenins (extracted in NaOH), 4 &ndash; glutenins (extracted in SDS) separated by SDS-PAGE were used as biochemical markers for evaluation of polymorphism level in three spelt wheat cultivars &ndash; Hercule, Altgold and&nbsp;Rouquin, three new&ndash;breeders&rsquo; spelt lines &ndash; H92.27, H92.28 and&nbsp;M92.20 (originated from hybridisation between spelt and common wheat) and reference common wheat cultivar Brea. Electrophoretic phenotypes and zymograms were evaluated by means of digital image analysis and Nei and Li coefficient of similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of all four-marker systems showed differences between common wheat cultivarBreaand spelt cultivars and spelt breeders&rsquo; lines. Also significant differences between old spelt cultivars (Hercule, Altgold and Rouquin) and new spelt breeders&rsquo; lines were found. The reality of the mutual passing of protein fractions (gliadins and glutenins), based on Osborne extraction was confirmed. In this sense it is necessary to see both fractions as dynamic overlapping structures.

scholarly journals Phytochemical Profile and Nutraceutical Value of Old and Modern Common Wheat Cultivars

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e45997 ◽  
Author(s):  
Emanuela Leoncini ◽  
Cecilia Prata ◽  
Marco Malaguti ◽  
Ilaria Marotti ◽  
Antonio Segura-Carretero ◽  
...  
Keyword(s):  
Common Wheat ◽  
Wheat Cultivars ◽  
Phytochemical Profile

scholarly journals Effect of Rothia mucilaginosa enzymes on gliadin (gluten) structure, deamidation, and immunogenic epitopes relevant to celiac disease

2014 ◽  
Vol 307 (8) ◽  
pp. G769-G776 ◽  
Author(s):  
Na Tian ◽  
Guoxian Wei ◽  
Detlef Schuppan ◽  
Eva J. Helmerhorst
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Mass Difference ◽  
Cross Linking ◽  
Esi Ms ◽  
Rp Hplc ◽  
Complementary Approach ◽  
Sds Page ◽  
Rothia Mucilaginosa ◽  
Immunogenic Properties

Rothia mucilaginosa, a natural microbial inhabitant of the oral cavity, cleaves gluten (gliadin) proteins at regions that are resistant to degradation by mammalian enzymes. The aim of this study was to investigate to what extent the R. mucilaginosa cell-associated enzymes abolish gliadin immunogenic properties. Degradation of total gliadins and highly immunogenic gliadin 33-mer or 26-mer peptides was monitored by SDS-PAGE and RP-HPLC, and fragments were sequenced by liquid chromatography and electrospray ionization tandem mass spectrometer (LC-ESI-MS/MS). Peptide deamidation by tissue transglutaminase (TG2), a critical step in rendering the fragments more immunogenic, was assessed by TG2-mediated cross-linking to monodansyl cadaverine (MDC), and by a +1-Da mass difference by LC-ESI-MS. Survival of potential immunogenic gliadin epitopes was determined by use of the R5 antibody-based ELISA. R. mucilaginosa-associated enzymes cleaved gliadins, 33-mer and 26-mer peptides into smaller fragments. TG2-mediated cross-linking showed a perfect inverse relationship with intact 33-mer and 26-mer peptide levels, and major degradation fragments showed a slow rate of MDC cross-linking of 6.18 ± 2.20 AU/min compared with 97.75 ± 10.72 and 84.17 ± 3.25 AU/min for the intact 33-mer and 26-mer, respectively, which was confirmed by reduced TG2-mediated deamidation of the fragments in mass spectrometry. Incubation of gliadins with Rothia cells reduced R5 antibody binding by 20, 82, and 97% after 30 min, 2 h, and 5 h, respectively, which was paralleled by reduced reactivity of enzyme-treated 33-mer and 26-mer peptides in the R5 competitive ELISA. Our broad complementary approach to validate gluten degrading activities qualifies R. mucilaginosa-associated enzymes as promising tools to neutralize T cell immunogenic properties for treatment of celiac disease.

scholarly journals Grain yield and competitive ability against weeds in modern and heritage common wheat cultivars are differently influenced by sowing density

2017 ◽  
Vol 11 ◽  
Author(s):  
Mariateresa Lazzaro ◽  
Ambrogio Costanzo ◽  
Dalia Hosam Farag ◽  
Paolo Bàrberi
Keyword(s):  
Grain Yield ◽  
Common Wheat ◽  
Weed Suppression ◽  
Strong Impact ◽  
Wheat Cultivars ◽  
Modern Cultivar ◽  
Weed Biomass ◽  
Growing Seasons ◽  
Viable Seeds ◽  
Crop Stand

Sowing density can have a strong impact on crop stand development during wheat growing cycle. In organic and low-input agriculture, and therefore with minimum or nil use of chemical herbicides, increased sowing density is expected to affect not only grain yield but also weed suppression. In this study we tested, under Mediterranean conditions, six common wheat cultivars (three modern and three heritage) and two three-component mixtures (arranged by combining the three modern or the three heritage cultivars). The different crop stands were tested at sowing densities of 250 (low) and 400 (high, similar to standard sowing density used by local farmers) viable seeds m-2 for two growing seasons. We did not detect a significant effect of crop stand diversity (single cultivars vs mixtures) on grain yield and weed suppression. Differences were ascribed to type of cultivars used (heritage vs modern). Compared to high sowing density, in modern cultivars grain yield did not decrease significantly with low sowing density whereas in heritage cultivars it increased by 15.6%, possibly also because of 21.5% lower plant lodging. Weed biomass increased with low sowing density both in heritage and modern cultivar crop stand types. However, heritage crop stands had, on average, a lower weed biomass (56%) than modern crop stands. Moreover, weed biomass in heritage crop stands at low density (6.82 ± 1.50 g m-2) was lower than that of modern cultivars at the same sowing density (15.54 ± 3.35 g m-2), confirming the higher suppressive potential of the former. We can conclude that lower sowing density can be advisable when using heritage crop stands as it keeps productivity while decreasing plant lodging and maintaining weeds under control.

scholarly journals Antioxidant, Antibacterial, and Cytoprotective Activity of Agathi Leaf Protein

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
A. S. Zarena ◽  
Shubha Gopal ◽  
R. Vineeth
Keyword(s):  
Safety Assessment ◽  
Crude Protein ◽  
Leaf Protein ◽  
Dependent Manner ◽  
Cytoprotective Activity ◽  
Sesbania Grandiflora ◽  
Rp Hplc ◽  
Maximum Inhibition ◽  
Sds Page ◽  
Dose Dependent

In the present study a protein termed agathi leaf protein (ALP) fromSesbania grandiflora Linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on Sephadex G 75. The column chromatography of the crude protein resulted in four peaks of which Peak I (P I) showed maximum inhibition activity against hydroxyl radical. SDS-PAGE analysis of P I indicated that the molecular weight of the protein is≈29 kDa. The purity of the protein was 98.4% as determined by RP-HPLC and showed a single peak with a retention time of 19.9 min. ALP was able to reduce oxidative damage by scavenging lipid peroxidation against erythrocyte ghost (85.50 ± 6.25%), linolenic acid (87.67 ± 3.14%) at 4.33 μM, ABTS anion (88 ± 3.22%), and DNA damage (83 ± 4.20%) at 3.44 μM in a dose-dependent manner. The purified protein offered significant protection to lymphocyte (72% at 30 min) induced damage by t-BOOH. In addition, ALP showed strong antibacterial activity againstPseudomonas aeruginosa(20 ± 3.64 mm) andStaphylococcus aureus(19 ± 1.53 mm) at 200 μg/mL. The safety assessment showed that ALP does not induce cytotoxicity towards human lymphocyte at the tested concentration of 0.8 mg/mL.

Distribution of Hybrid Necrosis Alleles in Genotypes of Aboriginal Common Wheat Cultivars from Afghanistan, Palestine, and Transjordan

2019 ◽  
Vol 55 (8) ◽  
pp. 955-961
Author(s):  
V. A. Pukhalskiy ◽  
E. V. Zuev ◽  
L. N. Bilinskaya ◽  
A. M. Kudryavtsev
Keyword(s):  
Common Wheat ◽  
Wheat Cultivars ◽  
Hybrid Necrosis

scholarly journals Variation in HMW glutenin subunits of different species of wheat

2011 ◽  
Vol 48 (No. 1) ◽  
pp. 15-19
Author(s):  
Z. Gálová ◽  
MichalíkI ◽  
H. Knoblochová ◽  
E. Gregová
Keyword(s):  
Triticum Turgidum ◽  
Wheat Cultivars ◽  
Glutenin Subunits ◽  
Bread Making ◽  
Wheat Varieties ◽  
Hmw Glutenin Subunits ◽  
Sds Page ◽  
Hmw Glutenin ◽  
Triticum Durum Desf

Method ISTA SDS-PAGE was used for separation, detection and evaluation of high molecular weight glutenin subunits (HMW) in the different wheat species. The relation has been studied between the HMW glutenin subunit alleles and the bread-making quality of 25 world wheat cultivars and 21 regional varieties common wheat varieties (Triticum aestivum L.), 17 winter spelt wheat (Triticum spelta L.), 3 durum wheat cultivars (Triticum durum DESF.), 9 cultivars of Triticum turgidum L. and 5 cultivars of Triticum polonicum L. The highest frequency of occurrence of HMW glutenin subunits 2*, 13 + 16 and 5 + 10 were found in world wheat cultivars. In Slovak wheat varieties were analysed subunits 0, 7 + 9 and 5 + 10, 2 + 12. The HMW subunits 0, 7 + 8 with Glu-score 4 were determined in Triticum durum DESF. Three electrophoretical profile groups of different HMW glutenin subunits were found in Triticum turgidum L. and Triticum polonicum L. and six electrophoretical profile groups were determined in Triticum spelta L. The verified correlations between bread-making quality and specific HMW subunits of glutenin can be utilised by wheat breeders using SDS-PAGE of proteins as a screening test for the prediction of bread-making quality of wheat.

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