scholarly journals Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3328 ◽  
Author(s):  
Song Chen ◽  
Peng Hou ◽  
Jingwen Sun ◽  
Haijun Wang ◽  
Lei Liu
Keyword(s):  
Response Time ◽  
Fluorescent Probe ◽  
Stokes Shift ◽  
Detection Limits ◽  
Fast Response ◽  
Living Cells ◽  
Pyridine Derivative ◽  
Selective Detection ◽  
Low Cytotoxicity ◽  
Mcf 7

A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, MIPY-DNBS was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.

scholarly journals A Phenothiazine-HPQ Based Fluorescent Probe with a Large Stokes Shift for Sensing Biothiols in Living Systems

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2337
Author(s):  
Yan Zheng ◽  
Peng Hou ◽  
Yu Li ◽  
Jingwen Sun ◽  
Hongxia Cui ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Enhancement ◽  
Stokes Shift ◽  
Redox Properties ◽  
Living Systems ◽  
Selective Detection ◽  
Large Stokes Shift ◽  
Low Cytotoxicity ◽  
Mcf 7

Due to the redox properties closely related to numerous physiological and pathological processes, biothiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), have received considerable attention in biological science. On account of the important physiological roles of these biothiols, it is of profound significance to develop sensitive and selective detection of biothiols to understand their biological profiles. In this work, we reported an efficient fluorescent probe, PHPQ-SH, for detecting biothiols in vitro and vivo, based on the phenothiazine-HPQ skeleton, with DNBS (2,4-dinitrobenzenesulfonate) as the response unit. Probe PHPQ-SH exhibited brilliant sensing performances toward thiols, including a large Stokes shift (138 nm), excellent sensitivity (for GSH, LOD = 18.3 nM), remarkable fluorescence enhancement (163-fold), low cytotoxicity, rapid response (8 min), and extraordinary selectivity. Finally, the probe PHPQ-SH illustrated herein was capable of responding and visualizing biothiols in MCF-7 cells and zebrafish.

A remarkable ratiometric fluorescent chemodosimeter for very rapid detection of hydrogen sulfide in the vapour phase and living cells

2015 ◽  
Vol 39 (11) ◽  
pp. 8940-8947 ◽  
Author(s):  
Sima Paul ◽  
Shyamaprosad Goswami ◽  
Chitrangada Das Mukhopadhyay
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Rapid Detection ◽  
Stokes Shift ◽  
Vapour Phase ◽  
Fast Response ◽  
Living Cells ◽  
Fluorescent Chemodosimeter ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe having a fast response and a large Stokes shift detects SH− both in solid and vapour phases and this probe is used for fluorescence imaging of SH− in living cells.

A novel near-infrared and naked-eyes turn on fluorescent probe for detection of biothiols with a large Stokes shift and its application in living cells

2018 ◽  
Vol 10 (32) ◽  
pp. 3991-3999 ◽  
Author(s):  
Wang Zhang ◽  
Gu Weijing ◽  
Tian Cheng ◽  
Bingxiang Wang ◽  
Yuliang Jiang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Stokes Shift ◽  
Living Cells ◽  
Selective Detection ◽  
Large Stokes Shift ◽  
Turn On

A novel turn-on fluorescent probe (DDND) for highly selective detection of biothiols over other amino acids was synthesized and investigated in this work, which used the 2,4-dinitrobenzenesulfonyl group as a fluorescent quencher.

A red-emitting fluorescent probe for the detection of Hg2+ in aqueous medium, living cells and organisms with a large Stokes shift

2018 ◽  
Vol 16 (27) ◽  
pp. 5036-5042 ◽  
Author(s):  
Lei Yang ◽  
Yuanan Su ◽  
Yani Geng ◽  
Haiqing Xiong ◽  
Jinliang Han ◽  
...  
Keyword(s):  
Detection Limit ◽  
Aqueous Solutions ◽  
Aqueous Medium ◽  
Fluorescent Probe ◽  
Stokes Shift ◽  
Living Cells ◽  
Selective Detection ◽  
Red Emission ◽  
Large Stokes Shift ◽  
Low Detection Limit

A vinethene-based fluorescent probe has been developed for the sensitive and selective detection of Hg2+ with a low detection limit, a red emission and a large Stokes shift. The probe has been successfully applied to detect Hg2+ in aqueous solutions, living cells and organisms.

scholarly journals A 3,5-dinitropyridin-2yl substituted naphthalimide-based fluorescent probe for the selective detection of biothiols and its application in cell-imaging

RSC Advances ◽  
2021 ◽  
Vol 11 (16) ◽  
pp. 9290-9295
Author(s):  
Yihua Zhuo ◽  
Yanyu Zhang ◽  
Yadong Feng ◽  
Yuqing Xu ◽  
Qihua You ◽  
...  
Keyword(s):  
Detection Limit ◽  
Response Time ◽  
Fluorescent Probe ◽  
Cell Imaging ◽  
Living Cells ◽  
Selective Detection ◽  
Satisfactory Response ◽  
Low Detection Limit

A highly selective OFF–ON fluorescent probe has been developed for the sensing of biothiols with a satisfactory response time and low detection limit. Also, the probe can be successfully applied for the sensing of biothiols in living cells.

A novel long-wavelength fluorescent probe for selective detection of hydrogen sulfide in living cells

2018 ◽  
Vol 42 (24) ◽  
pp. 19478-19484 ◽  
Author(s):  
Yu-ting Li ◽  
Xiong-jie Zhao ◽  
Yu-ren Jiang ◽  
Bing-qing Yang
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Long Wavelength ◽  
Low Cytotoxicity

Sensitive detection of endogenous H2S by a low cytotoxicity probe.

scholarly journals Design and Synthesis of a Fluorescent Probe with a Large Stokes Shift for Detecting Thiophenols and Its Application in Water Samples and Living Cells

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 375 ◽  
Author(s):  
Hua Liu ◽  
Chuanlong Guo ◽  
Shuju Guo ◽  
Lijun Wang ◽  
Dayong Shi
Keyword(s):  
Response Time ◽  
Metal Ions ◽  
Fluorescent Probe ◽  
Water Samples ◽  
Stokes Shift ◽  
Living Cells ◽  
Electron Transfer Mechanism ◽  
Short Response Time ◽  
Large Stokes Shift ◽  
Turn On

A turn-on florescent probe (probe-KCP) was developed for highly selective detection of thiophenols based on a donor-excited photo-induced electron transfer mechanism. Herein, the synthesis of the probe, a chalcone derivative, through a simple straightforward combination of a carbazole-chalcone fluorophore with a 2,4-dinitrophenyl functional group. In a kinetic study of the probe-KCP for thiophenols, the probe displayed a short response time (~30 min) and significant fluorescence enhancement. In selection and competition experiments, the probe-KCP exhibited excellent selectivity for thiophenols over glutathione (GSH), cysteine (Cys), sodium hydrosulfide (NaSH), and ethanethiol (C2H5SH) in addition to common anions and metal ions. Using the designed probe, we successfully monitored and quantified thiophenols, which are highly toxic. This turn-on fluorescence probe features a remarkably large Stokes shift (130 nm) and a short response time (30 min), and it is highly selective and sensitive (~160-fold) in the detection of thiophenols, with marked fluorescence in the presence of thiophenols. probe-KCP responds to thiophenols with a good range of linearity (0–15 μM) and a detection limit of 28 nM (R2 = 0.9946) over other tested species mentioned including aliphatic thiols, thiophenol analogues, common anions, and metal ions. The potential applications of this carbazole-chalcone fluorescent probe was successfully used to determine of thiophenols in real water samples and living cells with good performance and low cytotoxicity. Therefore, this probe has great potential application in environment and biological samples.

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