scholarly journals Chemical Profile and Multicomponent Quantitative Analysis for the Quality Evaluation of Toad Venom from Different Origins

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3595 ◽  
Author(s):  
Cao ◽  
Wu ◽  
Pan ◽  
Wang
Keyword(s):  
Quantitative Analysis ◽  
Quality Evaluation ◽  
Evaluation Method ◽  
Chemical Compositions ◽  
Hplc Fingerprint ◽  
Reference Information ◽  
Toad Venom ◽  
Different Origins ◽  
Tof Ms

Toad venom (Chansu), a traditional Chinese medicine (TCM), has been widely used for treating various cancer. However, it is considerably difficult to evaluate the quality of Chansu due to its complex chemical compositions. Hence, finding the characteristic ingredients and developing a scientific and comprehensive quality evaluation method are essential for guaranteeing the safety and efficacy of Chansu. In this paper, the chemical composition database of Chansu was successfully established and HPLC-ESI-Q-TOF-MS/MS was applied for chemical profiling of the ingredients in Chansu. In total, 157 compounds were identified, including 22 amino acids, 8 alkaloids, 54 bufogenins, 63 bufotoxins, and 10 other compounds. Furthermore, HPLC fingerprints and quantitative analysis of its multicomponent were successfully developed to evaluate the quality consistency of Chansu from different origins. The results suggested that the HPLC fingerprint of Chansu could be divided into an amino acid and alkaloid region, as well as a bufogenins and bufotoxins region. The fingerprint profile of Chansu from different geographical origins were different, indicating that its quality was affected by the geographical factors. In addition, seven characteristic peaks were selected as the quantitative markers to evaluate the quality of the Chansu. The Kruskal–Wallis test illustrated that the contents of seven bufogenins in Chansu were significantly (p < 0.01) different among different origins. The total contents of the seven compounds ranged from 100.40 to 169.22 mg/g in 20 batches of Chansu samples. This study demonstrated that integrating HPLC-ESI-Q-TOF-MS/MS, HPLC fingerprints, and multicomponent quantitative analysis coupled with chemometrics was a comprehensive and reliable strategy for evaluation of Chansu in both qualitative and quantitative aspects. In addition, our study represented the most comprehensive characterization on the chemical compositions of Chansu, which could provide important reference information for the discovery of potential bioactive compounds.

scholarly journals Development of HPLC Fingerprint for Quality Assessment of Bulbus Lilii

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801
Author(s):  
Kunming Qin ◽  
Hao Cai ◽  
Lijuan Zheng ◽  
Miao Zhang ◽  
Xinghai Zhang ◽  
...  
Keyword(s):  
High Performance ◽  
Quality Evaluation ◽  
Quality Analysis ◽  
Analysis Method ◽  
Hplc Fingerprint ◽  
Array Detection ◽  
Different Origins ◽  
First Time ◽  
And Storage

In this study, a simple, sensitive and reliable fingerprint analysis method by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for quality analysis of Bulbus Lilii, which is a famous traditional Chinese medicine and widely used as a restorative food. Then the developed method was subsequently applied to analyse 16 samples collected or purchased from different origins. Twelve common fingerprint peaks, including four definitely identified peaks, were found in total, and were involved for further characterisation and quality evaluation of Bulbus Lilii. The chemical quality of commercial samples collected from different origins varied obviously, indicating that more attention should be paid to the standardisation of the process of harvesting, manufacturing and storage of Bulbus Lilii. This is the first time that a new method has been established to control the quality of Bulbus Lilii through HPLC-DAD.

scholarly journals Analysis of the HPLC Fingerprint and QAMS for Sanhuang Gypsum Soup

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Yi Peng ◽  
Minghui Dong ◽  
Jing Zou ◽  
Zhihui Liu
Keyword(s):  
Quantitative Analysis ◽  
Standard Method ◽  
Evaluation Method ◽  
Similarity Analysis ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
External Standard ◽  
Single Marker

A valid and encyclopaedic evaluation method for assessing the quality of Sanhuang Gypsum Soup (SGS) has been set up based on analysis of high-performance liquid chromatography (HPLC) fingerprint combined with the quantitative analysis of multicomponents by single marker (QAMS) method, hierarchical cluster analysis (HCA), and similarity analysis. 20 peaks of the common model were obtained and used for the similarity analysis and HCA analysis. Berberine was selected as an internal reference, and the relative correction factors of mangiferin, geniposide, liquiritin, epiberberine, coptisine, baicalin, palmatine, harpagosid, wogonoside, cinnamic acid, cinnamic aldehyde, baicalein, glycyrrhizic acid, and wogonin were established. The accuracy of quantitative analysis of multicomponents by the single-marker method was verified by comparing the contents of the fourteen components calculated by the external standard method with those of the quantitative analysis of multicomponents by the single-marker method. No significant difference was found in the quantitative results of the established quantitative analysis of multicomponents by a single-marker method and an external standard method. In summary, these methods were applied to evaluate the quality of SGS successfully. As a result, these evaluation methods have great potential to be widely used in the quality control of traditional Chinese medicines (TCM).

Quality Assessment of Fructus xanthii Based on Fingerprinting Using High-Performance Liquid Chromatography

2012 ◽  
Vol 95 (4) ◽  
pp. 1053-1058 ◽  
Author(s):  
Liu Yang ◽  
Zhijun Su ◽  
Xing Zeng ◽  
Xiong Li ◽  
Zhifeng Wu ◽  
...  
Keyword(s):  
Quality Assessment ◽  
High Performance ◽  
Quality Evaluation ◽  
Evaluation Method ◽  
Multicomponent System ◽  
Principal Component ◽  
Uv Spectra ◽  
Hplc Fingerprint ◽  
Hplc Profiles

Abstract Because almost every traditional Chinese medicine (TCM) is a multicomponent system, QC of TCMs always involves various difficulties. As a current popular quality assessment approach, focusing on qualitative and quantitative analysis of certain compounds contained in herbal medicine has been widely used for the sake of expediency rather than being a practical and realistic way. However, this method does not take the existence of other constituents into account. Comparatively, the chromatographic fingerprint of the components is a more suitable approach to holistically assess the quality of herbal drugs. Fructus xanthii is a well-known herbal drug listed in all editions of the Chinese Pharmacopoeia. However, there is no quality evaluation method given in its monograph, even for the above-mentioned expediency. This paper reports an HPLC fingerprinting method for quality evaluation of F. xanthii. The HPLC profiles of 27 batches of commercial samples were further analyzed using chemometric methods, including similarity evaluation and principal component analysis. As a result, the established HPLC fingerprint contained 23 characteristic peaks; therein, 13 peaks were unambiguously assigned by comparing their retention times and UV spectra with those of reference compounds, and five peaks were tentatively identified on the basis of their MS/MS fragmentation patterns and UV spectra. Moreover, it could be clearly observed that caffeoylquinic acid and its analogs predominate in F. xanthii. Except for three samples identified as outliers, 24 other commercial samples displayed similar HPLC profiles, indicating that the quality of the herbs from different markets is stable and consistent.

scholarly journals HPLC Fingerprint Combined with Quantitation of Main Effective Components and Chemometrics as an Efficient Method for Quality Evaluation of Oviductus Ranae

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Shihan Wang ◽  
Yang Xu ◽  
Yanwei Wang ◽  
Zuying Lv ◽  
Qingyan Cui ◽  
...  
Keyword(s):  
High Performance ◽  
Quality Evaluation ◽  
Evaluation Method ◽  
Hierarchical Cluster ◽  
Liaoning Province ◽  
Hplc Fingerprint ◽  
Effective Components ◽  
Hplc Profiles ◽  
Oviductus Ranae

To ensure the highest quality of Oviductus Ranae products for consumers, an accurate, efficient, scientific and comprehensive evaluation method for assessing the quality of Oviductus Ranae was proposed in this paper. The fingerprint of Oviductus Ranae has been established based on the method of high performance liquid chromatography (HPLC). Oviductus Ranae from different habitats were analyzed by hierarchical cluster analysis (HCA). The HPLC profiles of fourteen batches of Oviductus Ranae showed 33 well-resolved common peaks in each chromatogram. Five of these were assigned to 1-methyl hydantoin, 7-ketocholesterol, 4-cholesten-3-one, cholesterol and stigmasterol. Contents of these five main effective components were determined in the meantime. Based on the HPLC profiles of all the samples, the reference HPLC fingerprint and similarity values were calculated by statistical software (SPSS 19.0). It was found that similarity value of all Oviductus Ranae samples were more than 0.85 and Oviductus Ranae from same habitats had similarity to some degree. In conclusion, the proposed method had good specificity, repeatability, stability and precision, which can provide a reference for the quality evaluation and identification of the medicine origin of Oviductus Ranae. Quantitation of effective components revealed that contents of five effective constituents in Oviductus Ranae obtained from Ji Lin province were the highest, followed by Hei Longjiang province and Liaoning province. The results of HCA showed that samples classified into one group were associated with their habitats. HPLC fingerprint combined with quantitation of main effective compounds and data analysis techniques can be an efficient and useful method for monitoring the quality of Oviductus Ranae.

Combinative method using multi-components quantitation by single reference standard and HPLC fingerprint for comprehensive evaluation of Rhodiola crenulata H.Ohba

2014 ◽  
Vol 6 (15) ◽  
pp. 5891-5898 ◽  
Author(s):  
Yiyang Du ◽  
Qing Li ◽  
Jingjing Liu ◽  
Yidi Yin ◽  
Kaishun Bi
Keyword(s):  
Quantitative Analysis ◽  
Comprehensive Evaluation ◽  
Reference Standard ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprint ◽  
Hplc Fingerprint ◽  
Rhodiola Crenulata ◽  
Different Origins

HPLC-DAD quantitative analysis and chromatographic fingerprint analysis were developed to evaluate the quality of Rhodiola crenulata H.Ohba from different origins.

scholarly journals Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1521 ◽  
Author(s):  
Yehong Li ◽  
Yiming Zhang ◽  
Zejun Zhang ◽  
Yupiao Hu ◽  
Xiuming Cui ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Evaluation Method ◽  
Herbal Medicines ◽  
Assessment Method ◽  
Gastrodia Elata ◽  
Clinical Safety ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines.

A new method to evaluate interlacing yarns

2019 ◽  
Vol 90 (7-8) ◽  
pp. 838-846
Author(s):  
Meng Wang ◽  
Yinlan Zhan ◽  
Leon Yao
Keyword(s):  
Coefficient Of Variation ◽  
Quality Evaluation ◽  
Evaluation Method ◽  
Complete Evaluation ◽  
Popular Method ◽  
Modeling Methods ◽  
The Third ◽  
Comparison And Analysis ◽  
Test Algorithm

The theory of purification is proposed in this article. Based on the framework of this theory, several models can be built to give a synthetic quality evaluation of interlacing yarns. In this paper, three models are given. The first one is the most popular method to evaluate the quality of interlacing yarn and we can prove it meets the theory of purification. The second one is modified according to the coefficient of variation. The third one is a new evaluation method. Meanwhile, we give a test algorithm to compare the degree of reflecting common cognition of the three models. From the result of comparison and analysis, the new evaluation method is recommended. It has a comparatively complete evaluation value for every state in universe M. Compared to other modeling methods, this method can give every level of interlacing yarn a relatively pertinent evaluation.

scholarly journals Blind Quality Evaluation for Screen Content Images Based on Regionalized Structural Features

Algorithms ◽  
2020 ◽  
Vol 13 (10) ◽  
pp. 257
Author(s):  
Wu Dong ◽  
Hongxia Bie ◽  
Likun Lu ◽  
Yeli Li
Keyword(s):  
Quality Evaluation ◽  
Evaluation Method ◽  
Structural Information ◽  
Modern Society ◽  
Structural Features ◽  
Human Vision ◽  
Natural Images ◽  
Histograms Of Oriented Gradients ◽  
Screen Content

Currently, screen content images (SCIs) are widely used in our modern society. However, since SCIs have distinctly different properties compared to natural images, traditional quality assessment methods of natural images cannot precisely evaluate the quality of SCIs. Thus, we propose a blind quality evaluation method for SCIs based on regionalized structural features that are closely relevant to the intrinsic quality of SCIs. Firstly, the features of textual and pictorial regions of SCIs are extracted separately. For textual regions, since they contain noticeable structural information, we propose improved histograms of oriented gradients extracted from multi-order derivatives as structural features. For pictorial regions, since human vision is sensitive to texture information and luminance variation, we adopt texture as the structural feature; meanwhile, luminance is used as the auxiliary feature. The local derivative pattern and the shearlet local binary pattern are used to extract texture in the spatial and shearlet domains, respectively. Secondly, to derive the quality of textual and pictorial regions, two mapping functions are respectively trained from their features to subjective values. Finally, an activity weighting strategy is proposed to combine the quality of textual and pictorial regions. Experimental results show that the proposed method achieves better performance than the state-of-the-art methods.

scholarly journals Chromatographic Fingerprint Combined with Content of Asperosaponin VI and Antioxidant Activity for Quality Evaluation of Wine-fried Dipsaci Radix

2014 ◽  
Vol 9 (6) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Li Song ◽  
Shaoyun Wang ◽  
Xiaoju Duan ◽  
Xinhua Liu ◽  
Zhaofang Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Quality Evaluation ◽  
Heating Temperature ◽  
Processing Condition ◽  
Influential Factor ◽  
Multivariate Statistical ◽  
Factors Affecting ◽  
Hplc Fingerprint

Dipsaci Radix, the dry root of Dipsacusasper Wall. ex Henry, is a commonly used traditional Chinese medicine (TCM). A novel comprehensive method was proposed for quality evaluation of wine-fried Dipsaci Radix by an integrated data including three aspects of information: high performance liquid chromatography (HPLC) fingerprint, asperosaponin VI content and antioxidant activity (AA). Various indicators including fingerprint similarity, asperosaponin VI content and AA were respectively employed for quality assessment of processed Dipsaci Radix samples. Results showed that considerable differences existed in quality of processed samples with different processing conditions according to three indicators. Among the factors affecting quality of wine-fried Dipsaci Radix, heating temperature was the most influential factor based on analysis of variance (ANOVA), and should be cautiously controlled. The three evaluation indicators respectively used for optimization of processing technology suggested different optimal conditions of wine-frying. Therefore, a combined indicator based on three evaluation indicators was used for determination of optimal processing condition. Multivariate statistical methods such as Hierarchical Clustering Analysis (HCA) and Principal Components Analysis (PCA) were both employed to classify the processed samples for quality evaluation. To more comprehensively evaluate the quality of wine-fried Dipsaci Radix, HPLC fingerprint combined with content of asperosaponin VI and AA may be a reasonable and practical approach.

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