scholarly journals Tranexamic acid quantification in human whole blood using liquid samples or volumetric absorptive microsampling devices

Bioanalysis ◽  
2020 ◽  
Author(s):  
Elodie Lamy ◽  
Ileana Runge ◽  
Ian Roberts ◽  
Haleema Shakur-Still ◽  
Stanislas Grassin-Delyle
Keyword(s):  
Tranexamic Acid ◽  
Whole Blood ◽  
International Association ◽  
Clinical Samples ◽  
European Medicines Agency ◽  
Therapeutic Drug ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Liquid Samples ◽  
Volumetric Absorptive Microsampling

Background: Recent clinical trials demonstrate the benefits of the antifibrinolytic drug tranexamic acid but its pharmacokinetics remain to be investigated more in depth. Although pharmacokinetics studies are usually performed with plasma, volumetric absorptive microsampling devices allow us to analyze dried whole blood samples with several advantages. Materials & methods: High-sensitivity LC–MS/MS methods for the quantification of tranexamic acid in human whole blood using liquid samples or dry samples on volumetric absorptive microsampling devices were developed and validated based on International Association from Therapeutic Drug Monitoring and Clinical Toxicology, European Medicines Agency and US Food and Drug Administration guidance. Conclusion: The method performances were excellent across the range of clinically relevant concentrations. The stability of tranexamic acid in blood samples stored up to 1 month at +50°C was demonstrated. The methods’ suitability was confirmed with clinical samples.

scholarly journals A whole blood microsampling assay for vancomycin: development, validation and application for pediatric clinical study

Bioanalysis ◽  
2020 ◽  
Vol 12 (18) ◽  
pp. 1295-1310
Author(s):  
Ganesh S Moorthy ◽  
Kevin J Downes ◽  
Christina Vedar ◽  
Athena F Zuppa
Keyword(s):  
Clinical Study ◽  
Therapeutic Drug Monitoring ◽  
Clinical Research ◽  
Clinical Data ◽  
Drug Monitoring ◽  
Whole Blood ◽  
Therapeutic Drug ◽  
Human Whole Blood ◽  
Assay Conditions ◽  
Volumetric Absorptive Microsampling

Background: Vancomycin is a commonly used antibiotic, which requires therapeutic drug monitoring to ensure optimal treatment. Microsampling assays are attractive tools for pediatric clinical research and therapeutic drug monitoring. Results: A LC–MS/MS method for the quantification of vancomycin in human whole blood employing volumetric absorptive microsampling (VAMS®) devices (20 μl) was developed and validated. Vancomycin was stable in human whole blood VAMS under assay conditions. Stability for vancomycin was established for at least 160 days as dried microsamples at -78°C. Conclusion: This method is currently being utilized for the quantitation of vancomycin in whole blood VAMS for an ongoing pediatric clinical study and representative clinical data are reported.

scholarly journals Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

2021 ◽  
Vol 2 (1) ◽  
pp. 100311
Author(s):  
Daniella C. Terenzi ◽  
Ehab Bakbak ◽  
Justin Z. Trac ◽  
Mohammad Al-Omran ◽  
Adrian Quan ◽  
...  
Keyword(s):  
Whole Blood ◽  
Progenitor Cell ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Isolation And Characterization ◽  
Whole Blood Samples

The distribution of BrKα/RbKα peak intensity ratio in human whole blood samples

1994 ◽  
Vol 42 (3) ◽  
pp. 231-241 ◽  
Author(s):  
C. Shenberg ◽  
S. Spiegel ◽  
S. Chaitchik ◽  
P. Jordan ◽  
M. Kitzis ◽  
...  
Keyword(s):  
Whole Blood ◽  
Intensity Ratio ◽  
Peak Intensity Ratio ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Peak Intensity ◽  
Whole Blood Samples

scholarly journals Volumetric Absorptive Microsampling of Blood for Untargeted Lipidomics

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 262
Author(s):  
Camilla Marasca ◽  
Maria Encarnacion Blanco Arana ◽  
Michele Protti ◽  
Andrea Cavalli ◽  
Laura Mercolini ◽  
...  
Keyword(s):  
High Resolution ◽  
Whole Blood ◽  
Chemical Space ◽  
Solid Support ◽  
Dried Blood Spot ◽  
Proof Of Concept ◽  
Viable Option ◽  
Human Whole Blood ◽  
Blood Spot ◽  
Volumetric Absorptive Microsampling

In the present, proof-of-concept paper, we explore the potential of one common solid support for blood microsampling (dried blood spot, DBS) and a device (volumetric absorptive microsampling, VAMS) developed for the untargeted lipidomic profiling of human whole blood, performed by high-resolution LC-MS/MS. Dried blood microsamples obtained by means of DBS and VAMS were extracted with different solvent compositions and compared with fluid blood to evaluate their efficiency in profiling the lipid chemical space in the most broad way. Although more effort is needed to better characterize this approach, our results indicate that VAMS is a viable option for untargeted studies and its use will bring all the corresponding known advantages in the field of lipidomics, such as haematocrit independence.

Evaluation of an electrochemical biosensor for uric acid measurement in human whole blood samples

2014 ◽  
Vol 436 ◽  
pp. 72-77 ◽  
Author(s):  
Li-Ting Liao ◽  
Chi-Chih Liao ◽  
Chiu-Ching Liu ◽  
Ting-Ya Yang ◽  
Giueng-Chueng Wang
Keyword(s):  
Uric Acid ◽  
Whole Blood ◽  
Electrochemical Biosensor ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Whole Blood Samples

Plasma vs whole blood for therapeutic drug monitoring of patients receiving FK 506 for immunosuppression

1994 ◽  
Vol 40 (12) ◽  
pp. 2247-2253 ◽  
Author(s):  
M Winkler ◽  
B Ringe ◽  
J Baumann ◽  
M Loss ◽  
K Wonigeit ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Enzyme Immunoassay ◽  
Drug Monitoring ◽  
Whole Blood ◽  
Plasma Concentrations ◽  
Therapeutic Monitoring ◽  
Therapeutic Drug ◽  
Fk 506 ◽  
Blood Samples ◽  
The Matrix

Abstract By retrospective analysis of 13,000 blood samples obtained from 248 patients receiving FK 506 therapy, we compared the suitability of plasma with that of whole blood as the matrix for therapeutic drug monitoring of FK 506. The plasma concentrations did not correlate with the concentrations in whole blood (r = 0.56). In contrast to plasma samples (analyzed by enzyme immunoassay), FK 506 was detectable in all whole-blood samples (analyzed by enzyme immunoassay/microparticle enzyme immunoassay). The inter- and intraindividual variations of FK 506 measurements were greater in plasma than in whole blood. Moreover, plasma concentrations correlated only poorly with clinical events. There was a tendency to greater plasma concentrations being measured during episodes of toxicity, but no clear difference was evident between stable course and rejection. In whole-blood specimens, a correlation between reduced or increased FK 506 concentrations and rejection or toxicity, respectively, was observed. The discriminatory power of whole-blood values was greater for the differentiation between toxicity and stable course than between rejection and stable course. We therefore recommend whole blood rather than plasma as the matrix for therapeutic monitoring of FK 506 concentrations.

Comparison of an electrochemical biosensor with optical devices for hemoglobin measurement in human whole blood samples

2011 ◽  
Vol 412 (23-24) ◽  
pp. 2150-2156 ◽  
Author(s):  
Ming-Song Hsieh ◽  
Tai-Guang Wu ◽  
Chein-Shyong Su ◽  
Wen-Jing Cheng ◽  
Namik Ozbek ◽  
...  
Keyword(s):  
Whole Blood ◽  
Electrochemical Biosensor ◽  
Optical Devices ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Whole Blood Samples ◽  
Hemoglobin Measurement
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