scholarly journals Content of Phenolic Acids in the Grain of Selected Polish Triticale Cultivars and Its Products

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 562
Author(s):  
Joanna Kaszuba ◽  
Ireneusz Kapusta ◽  
Zuzanna Posadzka
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ferulic Acid ◽  
Phenolic Acids ◽  
High Performance ◽  
Nutritive Value ◽  
Total Content ◽  
Fold Increase ◽  
Raw Material ◽  
Bread Making

The triticale grain has high nutritive value and good technological suitability. Triticale flour can be a valuable raw material for bread-making. The aim of this work was to determine the profile of phenolic acids in triticale grain of selected Polish cultivars and its products. Ultra-high-performance liquid chromatography (UPLC-PDA-MS/MS) was applied for separation and identification of these constituents. The grain of the examined triticale cultivars contained 13 phenolic acids, of which ferulic acid was determined in the largest amount and was constituted from 42–44% of the total content of phenolic acids in the grain. In addition, due to the large amounts of ferulic, di-ferulic, and sinapic acids, composition of the phenolic acids fraction in triticale grain of the tested cultivars varied in comparison with that of wheat and rye cultivars. In triticale flour, the number of phenolic acids was nearly 4 times lower than in the grain, as phenolic acids were removed along with bran, in which their proportion was almost 9 times higher than in the grain intended for grinding. The application of bran in the bread recipe resulted in a 3.5-fold increase in the fraction of phenolic acids compared to the bread produced from triticale flour without bran addition.

scholarly journals Chromatographical analysis of phenolic acids in some species of Polygonum L. genus. Quantitative determination of the major components by high performance liquid chromatography (HPLC)

2014 ◽  
Vol 69 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Helena Danuta Smolarz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Ferulic Acid ◽  
Phenolic Acids ◽  
High Performance ◽  
Protocatechuic Acid ◽  
Polygonum Persicaria

Nine major phenolic acids (protocatechuic, gentisic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, ferulic, synapic) were investigated in 9 species of the genus <em>Polygonum</em> L. The total amount of these compounds equated 65.8 µg/g of dry herb in <em>Polygonum persicaria</em> L., 61.2 µg in <em>P. convolvulus</em> L., 59.1 µg in <em>P. lapathifolium</em> ssp. <em>nodosum</em> (Pers.) Dans, 53.3 µg in <em>P. bistorta</em> L., 42.1 µg in <em>P. mite</em> Schrank, 38.2 µg in <em>P. lapathifolium</em> ssp. <em>tomentosum</em> (Schrank) Dans, 37 µg in <em>P. anrphibium</em> L., 33.2 µg in <em>P. hydropiper</em> L., 31.1 µg in <em>P. aviculare</em> L., and 14,1 µg in rhizome <em>P. bistorta</em> L. Among the analysed phenolic acids, synapic acid (28.6 µg/g) in <em>P. persicaria</em> L., protocatechuic acid (34 µg/g) in <em>P. lapathifolium</em> ssp. <em>nodosum</em> (Pers) Dans, and ferulic acid (21 µg/g) in <em>P. bistorta</em> L., were dominating.

scholarly journals Analysis of content of phenolic acids in Lithuanian propolis using high-performance liquid chromatography technique

Medicina ◽  
2009 ◽  
Vol 45 (9) ◽  
pp. 712 ◽  
Author(s):  
Kristina Ramanauskienė ◽  
Arūnas Savickas ◽  
Asta Inkėnienė ◽  
Konradas Vitkevičius ◽  
Giedrė Kasparavičienė ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Raw Material ◽  
Deciduous Trees ◽  
Analysis Method ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative

The aim of the study was to analyze phenolic acids in Lithuanian propolis and to compare it with the composition of propolis in neighboring countries (Latvia and Poland) according to the predominant flora in the collecting places. The study was also aimed at the evaluation of the effect of the layer thickness (mm) of the harvested propolis on the quality of the raw material in determining the amount of phenolic acids. Materials and methods. The object of the study was propolis collected in Lithuania, Poland, and Latvia in late July of 2006 and 2007. The qualitative and quantitative analysis of phenolic acids was performed using the high-performance liquid chromatography technique (HPLC). Results. The results of the study showed that the quantitative and qualitative composition of phenolic acids in propolis depended on the plants from which the bees in the area collected substances for the raw material of propolis. The predominant phenolic acids were determined to be ferulic and coumaric acids, and they may be among the main indicators of quality in the standardization of the raw material and preparations of propolis. Conclusion. We created an HPLC-based analysis method for the identification and quantification of phenolic acids in propolis. The variety of phenolic acids in propolis depends on the vegetation predominating in the harvesting area. Studies have shown that the highest amount of phenolic acids is observed in propolis harvested in areas characterized by the predominance of deciduous trees and meadows. Results have also shown that ferulic and coumaric acids are the predominant phenolic acids in propolis. The thickness of the layer of the collected propolis in the hive also influences its chemical composition.

Analysis of organic impurities of besifloxacin hydrochloride by high performance liquid chromatography with isocratic and gradient elution.

2019 ◽  
Vol 16 ◽  
Author(s):  
Joanna Wittckind Manoel ◽  
Camila Ferrazza Alves Giordani ◽  
Livia Maronesi Bueno ◽  
Sarah Chagas Campanharo ◽  
Elfrides Eva Sherman Schapoval ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Gradient Elution ◽  
Pharmaceutical Product ◽  
Raw Material ◽  
Isocratic Elution ◽  
Organic Impurities ◽  
Elution Method

Introduction: Impurity analysis is an important step in the quality control of pharmaceutical ingredients and final product. Impurities can arise from drug synthesis or excipients and even at small concentrations may affect product efficacy and safety. In this work two methods using high performance liquid chromatography (HPLC) were developed and validated for the evaluation of besifloxacin and its impurity synthesis, with isocratic elution and another with gradient elution. Method: The analysis by HPLC in isocratic elution mode was performed using a cyano column maintained at 25 °C. The mobile phase was composed by 0.5% triethylamine (pH 3.0): acetonitrile (88:12 v/v) eluted at a flow rate of 1.0 ml/min with detection at 330 nm. The gradient elution method was carried out with the same column and mobile phase components only modifying the rate between organic and aqueous phase during analysis. The procedures have been validated according to internationally accepted guidelines, observing results within acceptable limits. Results: The methods presented were found to be linear in the 140 to 260 µg/ml range for besifloxacin and 0.3 to 2.3 µg/ml for an impurity named A. The limits of detection and quantification were respectively 0.07 and 0.3 µg/ml for impurity A, with a 20 µL injection volume. The precision achieved for all analyses performed provided RSD inter-day equal to 6.47 and 6.36% for impurity A with isocratic elution and gradient, respectively. The accuracy was higher than 99% and robustness exhibited satisfactory results. In the isocratic method an analysis time of 25 min and 15 min was obtained for gradient. For impurity A, the number of theoretical plates in the isocratic mode was about 5000 while in the gradient mode it was about 45000, hence, it made the column more efficient by changing the mobile phase composition during elution. In besifloxacin raw material and in pharmaceutical product used in this study, other related impurities were present but but impurity A was searched for and not detected Conclusion: The proposed methods can be applied for quantitative determination of impurities in the analysis of the besifloxacin raw material, as well as in ophthalmic suspension of the drug, considering the quantitation limit.

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