scholarly journals Sugar Containing Compounds and Biological Activities of Lagochilus setulosus

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1755
Author(s):  
Davlat Kh. Akramov ◽  
Nilufar Z. Mamadalieva ◽  
Andrea Porzel ◽  
Hidayat Hussain ◽  
Mthandazo Dube ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Biological Activities ◽  
2D Nmr ◽  
Septoria Tritici ◽  
2D Nmr Spectroscopy ◽  
Aerial Parts ◽  
Spectroscopic Analyses ◽  
Phytochemical Investigation ◽  
Diphenyl Tetrazolium Bromide

Phytochemical investigation of the methanolic extract obtained from the aerial parts of Lagochilus setulosus (Lamiaceae) afforded the new compound 1-methoxy-3-O-β-glucopyranosyl-α-l-oliose (1) together with five known glycosides, namely sitosterol-3-O-β-glucoside (2), stigmasterol-3-O-β-glucoside (3), pinitol (4), 6β-hydroxyl-7-epi-loganin (5), and chlorotuberoside (6). The structures of these compounds were elucidated by extensive spectroscopic analyses, especially HR-MS, 1D and 2D NMR spectroscopy. The in vitro cytotoxic activity of the methanolic extract and the isolated compounds was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and crystal violet (CV) staining assays. In addition, the antifungal activities of the components were evaluated against Botrytis cinerea, Septoria tritici, and Phytophthora infestans. The anthelmintic potential was determined against Caenorhabditis elegans nematodes. Neither the extract nor the isolated compounds showed promising activity in all the bioassays.

scholarly journals Six New 3,5-Dimethylcoumarins from Chelonopsis praecox, Chelonopsis odontochila and Chelonopsis pseudobracteata

2021 ◽  
Author(s):  
Chang-An Geng ◽  
Zhen-Tao Deng ◽  
Qian Huang ◽  
Chun-Lei Xiang ◽  
Ji-Jun Chen
Keyword(s):  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
2D Nmr ◽  
Cell Protein ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine ◽  
Aerial Parts ◽  
Spectroscopic Analyses ◽  
New Compounds

AbstractTen 3,5-dimethylcoumarins (1–6 and 8‒11) involving six new ones (1–6), together with a known 3-methylcoumarin (7), were isolated from the aerial parts of three Chelonopsis plants, C. praecox, C. odontochila, and C. pseudobracteata. The structures of the new compounds were determined by extensive HRESIMS, 1D and 2D NMR spectroscopic analyses. According to the substitution at C-5, these coumarins were classified into 5-methyl, 5-hydroxymethyl, 5-formyl, and 5-nor types. All the isolates were assayed for their inhibition on α-glucosidase, protein tyrosine phosphatase 1B, and T-cell protein tyrosine phosphatase in vitro. Graphic Abstract

scholarly journals Four New Pterosins from Pteris cretica and Their Cytotoxic Activities

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2767
Author(s):  
Jian Lu ◽  
Caiying Peng ◽  
Shuang Cheng ◽  
Jianqun Liu ◽  
Qinge Ma ◽  
...  
Keyword(s):  
Human Tumor ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Aerial Parts ◽  
Pteris Cretica ◽  
Phytochemical Investigation ◽  
Hct 116 ◽  
Hct 116 Cells ◽  
New Compounds

Phytochemical investigation of the aerial parts of Pteris cretica led to the isolation and elucidation of nine pterosins, including four new pterosins, creticolacton A (1), 13-hydroxy-2(R),3(R)-pterosin L (2), creticoside A (3), and spelosin 3-O-β-d-glucopyranoside (4), together with five known pterosins 5–9. Their structures were identified mainly on the basis of 1D and 2D NMR spectral data, ESI-MS and literature comparisons. Compounds 1 and 3 were new type of petrosins with a six membered ring between C-14 and C-15. The new compounds were tested in vitro for their cytotoxic activities against four human tumor cell lines (SH-SY5Y, SGC-7901, HCT-116, Lovo). Results showed that compounds 1 and 2 exhibited cytotoxic activity against HCT-116 cells with IC50 value of 22.4 μM and 15.8 μM, respectively.

scholarly journals In VitroandIn VivoAntimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Farzaneh Naghibi ◽  
Somayeh Esmaeili ◽  
Noor Rain Abdullah ◽  
Mehdi Nateghpour ◽  
Mahdieh Taghvai ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Methanolic Extract ◽  
Cytotoxic Activities ◽  
Traditional Use ◽  
Aerial Parts ◽  
Mammalian Cell Lines ◽  
Phytochemical Investigation

Based on the collected ethnobotanical data from the Traditional Medicine and Materia Medica Research Center (TMRC), Iran,Myrtus communisL. (myrtle) was selected for the assessment ofin vitroandin vivoantimalarial and cytotoxic activities. Methanolic extract of myrtle was prepared from the aerial parts and assessed for antiplasmodial activity, using the parasite lactate dehydrogenase (pLDH) assay against chloroquine-resistant (K1) and chloroquine-sensitive (3D7) strains ofPlasmodium falciparum. The 4-day suppressive test was employed to determine the parasitemia suppression of the myrtle extract againstP. berghei  in vivo. The IC50values of myrtle extract were 35.44 µg/ml against K1 and 0.87 µg/ml against 3D7. Myrtle extract showed a significant suppression of parasitaemia (84.8 ± 1.1% at 10 mg/kg/day) in mice infected withP. bergheiafter 4 days of treatment. Cytotoxic activity was carried out against mammalian cell lines using methyl thiazol tetrazolium (MTT) assay. No cytotoxic effect on mammalian cell lines up to 100 µg/mL was shown. The results support the traditional use of myrtle in malaria. Phytochemical investigation and understanding the mechanism of action would be in our upcoming project.

NEW AROMATIC ALCOHOL, BENZOYL GLYCOSIDIC ESTER AND ANTHRAQUINONE DERIVATIVE FROM THE AERIAL PARTS OF PHYLLANTHUS FRATERNUS WEBSTER

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 40-44
Author(s):  
A Abuzer ◽  
◽  
M. Jameel ◽  
M. Ali
Keyword(s):  
Urinary Tract ◽  
Kidney Stones ◽  
Methanolic Extract ◽  
2D Nmr ◽  
Anthraquinone Derivative ◽  
Aromatic Alcohol ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Ft Ir ◽  
Phyllanthus Fraternus

Phyllanthus fraternus Webster (Euphorbiaceae) is used in Indian traditional systems of medicine to treat diabetes, influenza, dyspepsia, indigestion, dysentery, jaundice, kidney stones, urinary tract diseases, vaginitis and skin eruptions. Phytochemical investigation of a methanolic extract of aerial parts of P. fraternus led to the isolation of a new aromatic alcohol characterized as 3'-methyl-3'-(3,4- dimethoxyphenyl)-1'-propanol (1), a benzoyl glycosidic ester identified as 2-n-dodecanoyl-salicyloyl-O- β-D-arabinopyranoside (2) and a new anthraquinone derivative 1-methoxy-2,4,8,9,10,11-hexahydroxy- 3-methyl-6,13-anthracenedione (3) along with a known β-D-diglucoside (4). The structures of isolated phytoconstituents were established on the basis of 1D and 2D NMR, FT-IR, UV and MS data.

Three new coumarin types from aerial parts of Ammi majus L. and their cytotoxic activity

2018 ◽  
Vol 73 (1-2) ◽  
pp. 1-7 ◽  
Author(s):  
Eman El-Sharkawy ◽  
Yasser Selim
Keyword(s):  
High Activity ◽  
Hct116 Cell ◽  
2D Nmr ◽  
In Vitro Cytotoxicity ◽  
Spectroscopic Methods ◽  
Aerial Parts ◽  
Western Asia ◽  
Ammi Majus ◽  
Diphenyl Tetrazolium Bromide

AbstractThree new coumarin types were isolated from the aerial parts of the wild medicinal plantAmmi majus, which was collected from western Asia (Saudi Arabia), including pyrano coumarin, namely, 5-isobutylcoumarin-6-C-glucoside(1); furanocoumarin, namely, 6,7,9-Trimethoxy-3-(8′-methoxy-2′-oxo-2H-chromen-3-yl)-2H-furo[3,2-g]chromen-2(3H)-one(2); and pyrone coumarin, namely, 6-hydroxy-3-(2-hydroxypropyl)-7-methoxy-4 methyl coumarin(3). The structures were determined by spectroscopic methods, mainly 1D- and 2D-NMR. In vitro cytotoxicity was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The results of cytotoxicity depending on their structure features showed that compound3had high activity on different cell lines, while compound1showed no activity against HCT116. Compound2had high activity towards HCT116 cell line.

Two New Acylated Iridoid Glycosides from Verbascum undulatum

2000 ◽  
Vol 55 (7-8) ◽  
pp. 667-670 ◽  
Author(s):  
Prokopios Magiatis ◽  
Eleni Melliou ◽  
Eugenia Tsitsa ◽  
Catherine Charvala ◽  
Sofia Mitaku
Keyword(s):  
Nmr Spectroscopy ◽  
Spectral Methods ◽  
Iridoid Glycosides ◽  
2D Nmr ◽  
2D Nmr Spectroscopy ◽  
Aerial Parts ◽  
Phytochemical Investigation

Abstract On further phytochemical investigation of the aerial parts of Verbascum undulatum, two new acylated iridoid glycosides, 6-O-[3-O-(trans-3,4-dim ethoxycinnamoyl)-α- ʟ-rhamnopyranosyl] aucubin (1) and 6-O -[3-O-(trans-p-methoxycinnamoyl)-α-ʟ-rhamnopyranosyl]aucubin (2) were isolated. These structures were determined by spectral methods, mainly by 1D and 2D NMR spectroscopy.

scholarly journals Antiparasitic and Anticancer Carvotacetone Derivatives of Sphaeranthus bullatus

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700
Author(s):  
Francis Machumi ◽  
Abiy Yenesew ◽  
Jacob O. Midiwo ◽  
Matthias Heydenreich ◽  
Erich Kleinpeter ◽  
...  
Keyword(s):  
2D Nmr ◽  
Specific Rotation ◽  
Anticancer Activities ◽  
2D Nmr Spectroscopy ◽  
Cytotoxic Effects ◽  
H Nmr ◽  
Aerial Parts ◽  
Annual Herb ◽  
Derivatives Of

The CH2Cl2-MeOH (1:1) extract of the aerial parts of Sphaeranthus bullatus, an annual herb native to tropical East Africa, showed activity against chloroquine sensitive D6 (IC50 9.7 μg/mL) and chloroquine resistant W2 (IC50 15.0 μg/mL) strains of Plasmodium falciparum. Seventeen secondary metabolites were isolated from the extract through conventional chromatographic techniques and identified using various spectroscopic methods. The compounds were evaluated for their in vitro antiplasmodial, antileishmanial and anticancer activities revealing activity of four carvotacetone derivatives, namely 3-acetoxy-7-hydroxy-5-tigloyloxycarvotacetone (1), 3,7-dihydroxy-5-tigloyloxycarvotacetone (2), 3-acetoxy-5,7-dihydroxycarvotacetone (3) and 3,5,7-trihydroxy-carvotacetone (4); with antiplasmodial IC50 values of 1.40, 0.79, 0.60 and 3.40 μg/mL, respectively, against chloroquine sensitive D6 strains of P. falciparum; antiplasmodial activity of IC50 2.00, 0.90, 0.68 and 2.80 μg/mL, respectively, against chloroquine resistant W2 strains of P. falciparum; antileishmanial IC50 values of 0.70, 3.00, 0.70 and 17.00 μg/mL, respectively, against the parasite L. donovanii promastigotes, and anticancer activity against human SK-MEL, KB, BT-549 and SK-OV-3 tumor cells, with IC50 values between <1.1 - 5.3 μg/mL for 1-3. In addition, cytotoxic effects of the active compounds were evaluated against monkey kidney fibroblasts (VERO) and pig kidney epithelial cells (LLC-PK11). The structures of carvotacetone derivatives were determined by 1D and 2D NMR spectroscopy; the absolute stereochemical configuration of 3-acetoxy-7-hydroxy-5-tigloyloxycarvotacetone (1) was determined as 3 R, 4 R, 5 S by circular dichroism, specific rotation, 1H NMR and 2D NMR ROESY and NOESY experiments.

scholarly journals 2-Acetoxyverecynarmin C, a New Briarane COX Inhibitory Diterpenoid from Pennatula aculeata

2014 ◽  
Vol 9 (8) ◽  
pp. 1934578X1400900
Author(s):  
Anu Bahl ◽  
Sanjay M. Jachak ◽  
Kishneth Palaniveloo ◽  
Tulasiramanan Ramachandram ◽  
Charles S. Vairappan ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Furan Ring ◽  
Methanolic Extract ◽  
2D Nmr ◽  
2D Nmr Spectroscopy ◽  
Tricyclic Compound ◽  
2D Noesy ◽  
Relative Stereochemistry ◽  
Cox 1

A new briarane-type diterpenoid, named 2-acetoxyverecynarmin C, was isolated from the methanolic extract of an octocoral, Pennatula aculeata, that exhibited cyclooxygenase (COX) inhibitory activity. The structure of the compound was elucidated by ESI-HRMS, 1D and 2D NMR spectroscopy and comparison of the measured spectral data with those reported in the literature. The relative stereochemistry at chiral carbons was established from 2D NOESY experiments. 2-Acetoxyverecynarmin C is a tricyclic compound containing a furan ring at C-7,8 of a briarane skeleton. 2-Acetoxyverecynarmin C showed moderate inhibitory activity in in vitro COX-1 and COX-2 assays.

Chemical and Antiviral Study on Alkaloids from Papaver pseudocanescens M. Pop.

2012 ◽  
Vol 67 (1-2) ◽  
pp. 22-28 ◽  
Author(s):  
Ralitsa Istatkova ◽  
Lubomira Nikolaeva-Glomb ◽  
Angel Galabov ◽  
Gerelt-Od Yadamsuren ◽  
Javzan Samdan ◽  
...  
Keyword(s):  
Biological Activities ◽  
Antiviral Effect ◽  
Human Pathogens ◽  
Aerial Parts ◽  
Antiviral Effects ◽  
Benzylisoquinoline Alkaloid ◽  
Phytochemical Investigation ◽  
Taxonomic Groups ◽  
First Time

The phytochemical investigation of the aerial parts of Papaver pseudocanescens M. Pop. of Mongolian origin resulted in the isolation and structural elucidation of 8 alkaloids of the isoquinoline and promorphinane type. 8,14-Dihydroamurine, 8,14-dihydroflavinantine, and flavinantine are promorphinanes. Alborine, mecambridine, and mecambridine methohydroxide are retroprotoberberines. Amurensinine is an isopavine alkaloid and O-methylarmepavine is a benzylisoquinoline alkaloid. O-Methylarmepavine is a new alkaloid for the genus Papaver. Promorphinane-type alkaloids have been found for the first time in the species. All structures were established by physical and spectral analysis. As a first attempt to describe some of the biological activities of these alkaloids, the antiviral effect was tested against the in vitro replication of several viruses which belong to different taxonomic groups and represent significant human pathogens. Based on the results, the conclusion could be drawn that particular alkaloids from P. pseudocanescens possess selective antiviral effects against the replication of poliovirus 1 and human rhinovirus 14, two viruses from the Enterovirus genus of the Picornaviridae family.

scholarly journals Cyclic Polyketides with α-Glucosidase Inhibitory Activity from Endiandra kingiana Gamble and Molecular Docking Study

2021 ◽  
Vol 15 (5) ◽  
pp. 414-419
Author(s):  
Mohamad Nurul Azmi Mohamad Taib ◽  
Nur Amirah Saad ◽  
Mohamad Hafizi Abu Bakar ◽  
Mohammad Tasyriq Che Omar ◽  
Ahmad Nazif Aziz ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Active Site ◽  
Methanolic Extract ◽  
Docking Study ◽  
2D Nmr ◽  
Inhibition Activity ◽  
Molecular Docking Study ◽  
Phytochemical Investigation ◽  
Ic50 Value

A phytochemical investigation of the methanolic extract of Endiandra kingiana (bark) led to the isolation of four major compounds which are kingianic acid A (1), tsangibeilin B (2), kingianin A (3) and kingianin F (4). The structures were determined by 1D- and 2D-NMR analysis in combination with HRMS experiments. The compounds were screened for their in vitro α-glucosidase inhibition activity. Among them, compounds 3-4 showed potent α-glucosidase inhibition activity with IC50 value at 11.9 ± 2.0 µM and 19.7 ± 1.5 µM, respectively. The molecular docking study found that both compounds were bound into the active site of the N-terminal of MGAM, and thus agreed with the in vitro α-glucosidase enzyme inhibition activity results.

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