scholarly journals Lysozyme-like Protein Produced by Bifidobacterium longum Regulates Human Gut Microbiota Using In Vitro Models

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6480
Author(s):  
Mingzhu Du ◽  
Xinqiang Xie ◽  
Shuanghong Yang ◽  
Ying Li ◽  
Tong Jiang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Gut Microbiota ◽  
Bifidobacterium Longum ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
M1 Protein

The extracellular secreted protein of Bifidobacterium longum (B. longum) plays an important role in maintaining the homeostasis of the human intestinal microenvironment. However, the mechanism(s) of interaction remain unclear. Lysozyme is a kind of antibacterial peptide. In this study, the amino acid sequence of a lysozyme-like protein of B. longum based on whole-genome data of an isolate from human gut feces was found. We further predicted functional domains from the amino acid sequence, purified the protein, and verified its bioactivity. The growth of some bacteria were significantly delayed by the 020402_LYZ M1 protein. In addition, the gut microbiota was analyzed via high-throughput sequencing of 16S rRNA genes and an in vitro fermentation model, and the fluctuations in the gut microbiota under the treatment of 020402_LYZ M1 protein were characterized. The 020402_LYZ M1 protein affected the composition of human gut microbiota significantly, implying that the protein is able to communicate with intestinal microbes as a regulatory factor.

scholarly journals Revealing microbial species diversity using sequence capture by hybridization

2021 ◽  
Vol 7 (12) ◽  
Author(s):  
Sophie Marre ◽  
Cyrielle Gasc ◽  
Camille Forest ◽  
Yacine Lebbaoui ◽  
Pascale Mosoni ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rdna ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Taxonomic Resolution ◽  
Human Gut ◽  
Content Type ◽  
Innovative Strategy ◽  
Human Gut Microbiota ◽  
Rare Biosphere

Targeting small parts of the 16S rDNA phylogenetic marker by metabarcoding reveals microorganisms of interest but cannot achieve a taxonomic resolution at the species level, precluding further precise characterizations. To identify species behind operational taxonomic units (OTUs) of interest, even in the rare biosphere, we developed an innovative strategy using gene capture by hybridization. From three OTU sequences detected upon polyphenol supplementation and belonging to the rare biosphere of the human gut microbiota, we revealed 59 nearly full-length 16S rRNA genes, highlighting high bacterial diversity hidden behind OTUs while evidencing novel taxa. Inside each OTU, revealed 16S rDNA sequences could be highly distant from each other with similarities down to 85 %. We identified one new family belonging to the order Clostridiales , 39 new genera and 52 novel species. Related bacteria potentially involved in polyphenol degradation have also been identified through genome mining and our results suggest that the human gut microbiota could be much more diverse than previously thought.

In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin

2019 ◽  
Vol 120 ◽  
pp. 595-602 ◽  
Author(s):  
Fernanda Bianchi ◽  
Nadja Larsen ◽  
Thatiana de Mello Tieghi ◽  
Maria Angela T. Adorno ◽  
Susana M.I. Saad ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Bifidobacterium Longum ◽  
Microbiota Composition ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Gut Microbiota Composition

In vitro study of the interaction between human gut microbiota and herbal extracts using willow bark extract as an example

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
EM Pferschy-Wenzig ◽  
K Koskinen ◽  
C Moissl-Eichinger ◽  
R Bauer
Keyword(s):  
Gut Microbiota ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bark Extract ◽  
Herbal Extracts ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Willow Bark

Metabolization of the herbal combination STW-5 by human gut microbiota in vitro

2017 ◽  
Author(s):  
EM Pferschy-Wenzig ◽  
A Roßmann ◽  
K Koskinen ◽  
H Abdel-Aziz ◽  
C Moissl-Eichinger ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
Human Gut Microbiota

Analysis of in vitro digestion using human gut microbiota in adult and elderly individuals

2021 ◽  
pp. 130228
Author(s):  
Seung Yun Lee ◽  
Da Young Lee ◽  
Hea Jin Kang ◽  
Ji Hyeop Kang ◽  
Hae Won Jang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Vitro Digestion ◽  
Human Gut ◽  
Elderly Individuals ◽  
Human Gut Microbiota

In Vitro Effects of Stachyose on the Human Gut Microbiota

2021 ◽  
pp. 2100029
Author(s):  
Zhonglin Zhao ◽  
Wei Liu ◽  
Xionge Pi
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
In Vitro Effects

scholarly journals Effects of phlorotannin and polysaccharide fractions of brown seaweed Silvetia compressa on human gut microbiota composition using an in vitro colonic model

2021 ◽  
Vol 84 ◽  
pp. 104596
Author(s):  
Benjamín Vázquez-Rodríguez ◽  
Liliana Santos-Zea ◽  
Erick Heredia-Olea ◽  
Laura Acevedo-Pacheco ◽  
Arlette Santacruz ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Brown Seaweed ◽  
Microbiota Composition ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Gut Microbiota Composition

A pectic polysaccharide from peach palm fruits (Bactris gasipaes) and its fermentation profile by the human gut microbiota in vitro

2017 ◽  
Vol 9 ◽  
pp. 1-6 ◽  
Author(s):  
Thaisa Moro Cantu-Jungles ◽  
Thales R. Cipriani ◽  
Marcello Iacomini ◽  
Bruce R. Hamaker ◽  
Lucimara M.C. Cordeiro
Keyword(s):  
Gut Microbiota ◽  
Bactris Gasipaes ◽  
Human Gut ◽  
Pectic Polysaccharide ◽  
Peach Palm ◽  
Human Gut Microbiota ◽  
Fermentation Profile ◽  
Palm Fruits

Effects of exopolysaccharide from Lactobacillus rhamnosus on human gut microbiota in in vitro fermentation model

LWT ◽  
2020 ◽  
pp. 110524
Author(s):  
Yuzhu Zhu ◽  
Jia-Min Zhou ◽  
Wei Liu ◽  
Xionge Pi ◽  
Qingqing Zhou ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Lactobacillus Rhamnosus ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Human Gut Microbiota ◽  
Fermentation Model
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