scholarly journals Grafting TRAIL through Either Amino or Carboxylic Groups onto Maghemite Nanoparticles: Influence on Pro-Apoptotic Efficiency

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 502
Author(s):  
Hanene Belkahla ◽  
Andrei Alexandru Constantinescu ◽  
Tijani Gharbi ◽  
Florent Barbault ◽  
Alexandre Chevillot-Biraud ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Cancer Therapy ◽  
Computational Study ◽  
Carcinoma Cells ◽  
Maghemite Nanoparticles ◽  
Carboxylic Acid Groups ◽  
Lung Carcinoma Cells ◽  
Biological Studies ◽  
Apoptotic Effect ◽  
Carboxylic Groups

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF cytokine superfamily. TRAIL is able to induce apoptosis through engagement of its death receptors DR4 and DR5 in a wide variety of tumor cells while sparing vital normal cells. This makes it a promising agent for cancer therapy. Here, we present two different ways of covalently grafting TRAIL onto maghemite nanoparticles (NPs): (a) by using carboxylic acid groups of the protein to graft it onto maghemite NPs previously functionalized with amino groups, and (b) by using the amino functions of the protein to graft it onto NPs functionalized with carboxylic acid groups. The two resulting nanovectors, NH-TRAIL@NPs-CO and CO-TRAIL@NPs-NH, were thoroughly characterized. Biological studies performed on human breast and lung carcinoma cells (MDA-MB-231 and H1703 cell lines) established these nanovectors are potential agents for cancer therapy. The pro-apoptotic effect is somewhat greater for CO-TRAIL@NPs-NH than NH-TRAIL@NPs-CO, as evidenced by viability studies and apoptosis analysis. A computational study indicated that regardless of whether TRAIL is attached to NPs through an acid or an amino group, DR4 recognition is not affected in either case.

Apoptotic Effect of Galbanic Acid via Activation of Caspases and Inhibition of Mcl-1 in H460 Non-Small Lung Carcinoma Cells

2015 ◽  
Vol 29 (6) ◽  
pp. 844-849 ◽  
Author(s):  
Bum-Seok Oh ◽  
Eun Ah Shin ◽  
Ji Hoon Jung ◽  
Deok-Beom Jung ◽  
Bonglee Kim ◽  
...  
Keyword(s):  
Lung Carcinoma ◽  
Carcinoma Cells ◽  
Lung Carcinoma Cells ◽  
Apoptotic Effect ◽  
Galbanic Acid

Die Hydratation von Carboxyl-Gruppen in Ionenaustauscherharzen Eine dielektrische Untersuchung / The Hydration of Carboxylic Acid Groups in Ion Exchange Resins A Dielectric Investigation

1970 ◽  
Vol 25 (8) ◽  
pp. 808-820 ◽  
Author(s):  
K. Bunzl ◽  
B. Sansoni
Keyword(s):  
Dielectric Constant ◽  
Carboxylic Acid ◽  
Ion Exchange ◽  
Water Content ◽  
Ion Exchange Resins ◽  
Dielectric Measurements ◽  
Proton Mobility ◽  
Carboxylic Acid Groups ◽  
Carboxylic Groups ◽  
Exchange Resins

The hydration of carboxylic acid groups in ion exchange resins was studied by means of dielectric measurements. For three resins carrying carboxylic groups with quite different pK-values the dielectric constant and the dielectric loss were measured as a function of frequency, water content, and temperature. A comparison of their behaviour showed that the observed dielectric dispersion is due to a Maxwell-Wagner mechanism. In the course of the water adsorption the resin with very weak carboxylic acid groups exhibited a discontinuity of the dielectric constant, if one molecule of water was attached to two carboxylic groups. This can be explained by assuming that the carboxylic groups which are connected by hydrogen bonds in the dry state begin to dissociate at the above mentioned water content. As a consequence, the formation of H5O2⊕-complexes becomes possible. The corresponding increase of the proton mobility leads to the observed Maxwell-Wagner effects. Hydration structures of the carboxylic acid groups below and above this critical water content are proposed.In the case of the two other resins with higher acidity of the carboxyl groups no discontinuity during water sorption could be observed. The mobility of the proton and hence the Maxwell-Wagner effects could thus be observed at much lower water contents.The appreciable differences in the shapes of the corresponding wateradsorption isotherms are in agreement with conclusions from the dielectric measurements.

scholarly journals Fer and FerT Govern Mitochondrial Susceptibility to Metformin and Hypoxic Stress in Colon and Lung Carcinoma Cells

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 97
Author(s):  
Odeya Marciano ◽  
Linoy Mehazri ◽  
Sally Shpungin ◽  
Alexander Varvak ◽  
Eldad Zacksenhaus ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Aerobic Glycolysis ◽  
Metastatic Cancer ◽  
Carcinoma Cells ◽  
Metabolic Adaptation ◽  
Hypoxic Stress ◽  
Lung Carcinoma Cells ◽  
Metabolic Role ◽  
Highly Sensitive ◽  
Anticancer Treatment

Aerobic glycolysis is an important metabolic adaptation of cancer cells. However, there is growing evidence that reprogrammed mitochondria also play an important metabolic role in metastatic dissemination. Two constituents of the reprogrammed mitochondria of cancer cells are the intracellular tyrosine kinase Fer and its cancer- and sperm-specific variant, FerT. Here, we show that Fer and FerT control mitochondrial susceptibility to therapeutic and hypoxic stress in metastatic colon (SW620) and non-small cell lung cancer (NSCLC-H1299) cells. Fer- and FerT-deficient SW620 and H1299 cells (SW∆Fer/FerT and H∆Fer/FerT cells, respectively) become highly sensitive to metformin treatment and to hypoxia under glucose-restrictive conditions. Metformin impaired mitochondrial functioning that was accompanied by ATP deficiency and robust death in SW∆Fer/FerT and H∆Fer/FerT cells compared to the parental SW620 and H1299 cells. Notably, selective knockout of the fer gene without affecting FerT expression reduced sensitivity to metformin and hypoxia seen in SW∆Fer/FerT cells. Thus, Fer and FerT modulate the mitochondrial susceptibility of metastatic cancer cells to hypoxia and metformin. Targeting Fer/FerT may therefore provide a novel anticancer treatment by efficient, selective, and more versatile disruption of mitochondrial function in malignant cells.

scholarly journals Two distinct bombesin receptor subtypes are expressed and functional in human lung carcinoma cells.

1991 ◽  
Vol 266 (28) ◽  
pp. 18771-18779
Author(s):  
M.H. Corjay ◽  
D.J. Dobrzanski ◽  
J.M. Way ◽  
J. Viallet ◽  
H. Shapira ◽  
...  
Keyword(s):  
Lung Carcinoma ◽  
Human Lung ◽  
Carcinoma Cells ◽  
Receptor Subtypes ◽  
Lung Carcinoma Cells ◽  
Human Lung Carcinoma ◽  
Bombesin Receptor

scholarly journals Application of self-assembly peptides targeting the mitochondria as a novel treatment for sorafenib-resistant hepatocellular carcinoma cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tae Ho Hong ◽  
M. T. Jeena ◽  
Ok-Hee Kim ◽  
Kee-Hwan Kim ◽  
Ho Joong Choi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Antioxidant Enzymes ◽  
Self Assembly ◽  
Carcinoma Cells ◽  
Anticancer Activities ◽  
Anticancer Effects ◽  
Novel Treatment ◽  
Mitochondrial Ros ◽  
Appropriate Treatment ◽  
Apoptotic Effect

AbstractCurrently, there is no appropriate treatment option for patients with sorafenib-resistant hepatocellular carcinoma (HCC). Meanwhile, pronounced anticancer activities of newly-developed mitochondria-accumulating self-assembly peptides (Mito-FF) have been demonstrated. This study intended to determine the anticancer effects of Mito-FF against sorafenib-resistant Huh7 (Huh7-R) cells. Compared to sorafenib, Mito-FF led to the generation of relatively higher amounts of mitochondrial reactive oxygen species (ROS) as well as the greater reduction in the expression of antioxidant enzymes (P < 0.05). Mito-FF was found to significantly promote cell apoptosis while inhibiting cell proliferation of Huh7-R cells. Mito-FF also reduces the expression of antioxidant enzymes while significantly increasing mitochondrial ROS in Huh7-R cells. The pro-apoptotic effect of Mito-FFs for Huh7-R cells is possibly caused by their up-regulation of mitochondrial ROS, which is caused by the destruction of the mitochondria of HCC cells.

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