scholarly journals Comparison of Duplex and Quadruplex Folding Structure Adenosine Aptamers for Carbon Nanotube Field Effect Transistor Aptasensors

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2280
Author(s):  
Hong Phan T. Nguyen ◽  
Thanihaichelvan Murugathas ◽  
Natalie O. V. Plank
Keyword(s):  
Carbon Nanotube ◽  
Field Effect ◽  
High Performance ◽  
Field Effect Transistor ◽  
Dissociation Constants ◽  
High Sensitivity ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Effect Transistor ◽  
Folding Structure

Carbon nanotube field effect transistor (CNT FET) aptasensors have been investigated for the detection of adenosine using two different aptamer sequences, a 35-mer and a 27-mer. We found limits of detection for adenosine of 100 pM and 320 nM for the 35-mer and 27-mer aptamers, with dissociation constants of 1.2 nM and 160 nM, respectively. Upon analyte recognition the 35-mer adenosine aptamer adopts a compact G-quadruplex structure while the 27-mer adenosine aptamer changes to a folded duplex. Using the CNT FET aptasensor platform adenosine could be detected with high sensitivity over the range of 100 pM to 10 µM, highlighting the suitability of the CNT FET aptasensor platform for high performance adenosine detection. The aptamer restructuring format is critical for high sensitivity with the G-quadraplex aptasensor having a 130-fold smaller dissociation constant than the duplex forming aptasensor.

scholarly journals Real-Time Monitoring of a Botulinum Neurotoxin Using All-Carbon Nanotube-Based Field-Effect Transistor Devices

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4235 ◽  
Author(s):  
Nam Lee ◽  
Seung-Hoon Nahm ◽  
Insung Choi
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Field Effect ◽  
Botulinum Neurotoxin ◽  
Field Effect Transistor ◽  
Detection System ◽  
High Sensitivity ◽  
Real Time Monitoring ◽  
Unique Peptide ◽  
Effect Transistor

The possibility of exposure to botulinum neurotoxin (BoNT), a powerful and potential bioterrorism agent, is considered to be ever increasing. The current gold-standard assay, live-mouse lethality, exhibits high sensitivity but has limitations including long assay times, whereas other assays evince rapidity but lack factors such as real-time monitoring or portability. In this study, we aimed to devise a novel detection system that could detect BoNT at below-nanomolar concentrations in the form of a stretchable biosensor. We used a field-effect transistor with a p-type channel and electrodes, along with a channel comprising aligned carbon nanotube layers to detect the type E light chain of BoNT (BoNT/E-Lc). The detection of BoNT/E-Lc entailed observing the cleavage of a unique peptide and the specific bonding between BoNT/E-Lc and antibody BoNT/E-Lc (Anti-BoNT/E-Lc). The unique peptide was cleaved by 60 pM BoNT/E-Lc; notably, 52 fM BoNT/E-Lc was detected within 1 min in the device with the antibody in the bent state. These results demonstrated that an all-carbon nanotube-based device (all-CNT-based device) could be produced without a complicated fabrication process and could be used as a biosensor with high sensitivity, suggesting its potential development as a wearable BoNT biosensor.

Analytical modeling of high performance single-walled carbon nanotube field-effect-transistor

2010 ◽  
Vol 41 (9) ◽  
pp. 579-584 ◽  
Author(s):  
Desmond C.Y. Chek ◽  
Michael L.P. Tan ◽  
Mohammad Taghi Ahmadi ◽  
Razali Ismail ◽  
Vijay K. Arora
Keyword(s):  
Carbon Nanotube ◽  
Field Effect ◽  
High Performance ◽  
Analytical Modeling ◽  
Field Effect Transistor ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Effect Transistor
Sign in / Sign up

Export Citation Format

Share Document