Identification and Characterization of Edible Cricket Peptides on Hypertensive and Glycemic In Vitro Inhibition and Their Anti-Inflammatory Activity on RAW 264.7 Macrophage Cells

Recent studies continue to demonstrate the potential of edible insects as a protein base to obtain bioactive peptides applicable for functional food development. This study aimed at identifying antihypertensive, anti-glycemic, and anti-inflammatory peptides derived from the in vitro gastrointestinal digests of cricket protein hydrolysates. After sequential fractionation, the protein digest subfraction containing the lowest molecular weight (<0.5 kDa), hydrophobic (C18) and cationic peptides (IEX) was found responsible for the most bioactivity. The cationic peptide fraction significantly reduced (p < 0.05) α-amylase, α-glucosidase, and angiotensin converting enzyme (ACE) activity in vitro, and also inhibited the expression of NF-κB in RAW 264.7 macrophage cells. A total of 28 peptides were identified with mass spectrometry (LC–MS/MS) and de novo sequencing from the potent fraction. Three novel peptides YKPRP, PHGAP, and VGPPQ were chosen for the molecular docking studies. PHGAP and VGPPQ exhibited a higher degree of non-covalent interactions with the enzyme active site residues and binding energies comparable to captopril. Results from this study demonstrate the bioactive potential of edible cricket peptides, especially as ACE inhibitors.

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Optimization of an anti-inflammatory screening model on the RAW 264.7 macrophage cell

Science and Technology Development Journal - Natural Sciences ◽

10.32508/stdjns.v1it5.531 ◽

2018 ◽

Vol 1 (T5) ◽

pp. 18-26

Author(s):

To Dinh Le ◽

Kien Trung Nguyen ◽

Thuoc Linh Tran ◽

Thao Thi Phuong Dang

Keyword(s):

New Drugs ◽

No Production ◽

Raw 264.7 ◽

Macrophage Cells ◽

Raw 264.7 Macrophage ◽

Model Cell ◽

Raw 264.7 Macrophage Cells ◽

Anti Inflammatory ◽

Living Tissues

Inflammation is the response of living tissues to the injury. Prolonged or inappropriate inflammation has been involved with many diseases such as, cancer, diabetes, heart disease… These days, inflammation has been treated by nonsteroidal and steroidal anti-inflammatory drugs, which have a lot of side effects. It opens the need and interest of new drugs discovery. Particularly, scientific and pharmaceutical communities show great interest in finding new anti-inflammatory compounds in traditional medicinal plants. This study aimed to optimize an in vitro anti-inflammatory model. Cell heterogeneity, cell density, LPS concentration and LPS incubation time were chosen to optimize the production of nitric oxide (NO) in RAW 264.7 macrophage cells. Our results show that 104 cells/well, FBS 1 %, starvation for 6 h, LPS 0.5 µg/mL in 24 h were optimized parameters in the model. Then, extracts from Hedyotis capitellata, a traditional medicinal plant used by K’ho minority, Bidoup Nuiba national park, Lam Dong province, Vietnam, was chosen to evaluate the in vitro antiinflammatory model. The anti-inflammatory activity was tested by measuring the production of NO in lipopolysaccharide-activated RAW 264.7 macrophage cells. The experimental data indicate that the extracts of this plant decreased NO production in LPS-stimulated RAW 264.7, particularly, the petroleum ether fraction at the concentration of 23.8 µg/mL inhibited NO production by 128.20 %; whereas dexamethasone 50 µM inhibited NO accumulation to 112 %.

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