scholarly journals Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1250
Author(s):  
Sílvia da Silva Fontes ◽  
Fernanda de Moraes Maia ◽  
Laura Santa’Anna Ataides ◽  
Fernando Paiva Conte ◽  
Josué da Costa Lima-Junior ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
B Cell ◽  
Outer Membrane ◽  
Q Fever ◽  
Outer Membrane Proteins ◽  
Humoral Response ◽  
Neglected Diseases ◽  
B Cell Epitopes ◽  
Wide Range ◽  
Linear B

Coxiella burnetii is a global, highly infectious intracellular bacterium, able to infect a wide range of hosts and to persist for months in the environment. It is the etiological agent of Q fever—a zoonosis of global priority. Currently, there are no national surveillance data on C. burnetii’s seroprevalence for any South American country, reinforcing the necessity of developing novel and inexpensive serological tools to monitor the prevalence of infections among humans and animals—especially cattle, goats, and sheep. In this study, we used immunoinformatics and computational biology tools to predict specific linear B-cell epitopes in three C. burnetii outer membrane proteins: OMP-H (CBU_0612), Com-1 (CBU_1910), and OMP-P1 (CBU_0311). Furthermore, predicted epitopes were tested by ELISA, as synthetic peptides, against samples of patients reactive to C. burnetii in indirect immunofluorescence assay, in order to evaluate their natural immunogenicity. In this way, two linear B-cell epitopes were identified in each studied protein (OMP-H(51–59), OMP-H(91–106), Com-1(57–76), Com-1(191–206), OMP-P1(197–209), and OMP-P1(215–227)); all of them were confirmed as naturally immunogenic by the presence of specific antibodies in 77% of studied patients against at least one of the identified epitopes. Remarkably, a higher frequency of endocarditis cases was observed among patients who presented an intense humoral response to OMP-H and Com-1 epitopes. These data confirm that immunoinformatics applied to the identification of specific B-cell epitopes can be an effective strategy to improve and accelerate the development of surveillance tools against neglected diseases.

scholarly journals In Silico Epitope-Based Peptide Vaccine Design against Invasive Non Typhoidal Salmonella (iNTS) Through Immunoinformatic Approaches

2020 ◽  
Author(s):  
Md. Chayan Ali ◽  
Sultana Israt Jahan ◽  
Mst. Shanzeda Khatun ◽  
Raju Das ◽  
Md Mafizur Rahman ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
B Cell ◽  
Outer Membrane ◽  
Vaccine Candidate ◽  
Binding Free Energy ◽  
Outer Membrane Proteins ◽  
Peptide Vaccine ◽  
Dynamics Simulation ◽  
Mhc I ◽  
B Cell Epitopes

Salmonella, especially invasive non-typhoidal Salmonella (iNTS) are responsible for developing various invasive diseases, and possess higher mortality rate, due to their higher antibiotic resistance profile than the other bacteria. Therefore, the present study was concerned to develop epitope based peptide vaccine against iNTS species as a successive and substitute protective measures. The study considered comprehensive Immunoinformatic approaches, followed by molecular docking and molecular dynamics simulation to predict the efficient vaccine candidate T cell and B cell epitopes, based on the outer membrane proteins. The study identified two best epitopes YGIFAITAL and KVLYGIFAI from total iNTS outer membrane proteins, which showed higher immunity, non-allergenicity, non-toxicity and also showed higher conservancy and population coverage values. Both epitopes showed higher binding affinity and stability towards HLA-C* 03:03. The MM-PBSA binding free energy showed the YGIFAITAL epitope binds more tightly with both MHC-I and MHC-II molecules. The total contact, H-bond analysis and RMSF results also validate the efficiency of these epitopes as vaccine candidate. The projected B cell epitopes AAPVQVGEAAGS, TGGGDGSNT and TGGGDGSNTGTTTT showed higher antigenicity. Overall, the study concluded that these epitopes can be considered as the potential vaccine candidate to make a successive vaccine against iNTS species. However, this result further needs to be validate by wet lab research to make successive vaccine with these projected epitopes.

scholarly journals Characterization of the Outer Membrane Proteome of Leptospira interrogans Expressed during Acute Lethal Infection

2006 ◽  
Vol 75 (2) ◽  
pp. 766-773 ◽  
Author(s):  
Jarlath E. Nally ◽  
Julian P. Whitelegge ◽  
Sara Bassilian ◽  
David R. Blanco ◽  
Michael A. Lovett
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Disease Transmission ◽  
Guinea Pigs ◽  
Outer Membrane Proteins ◽  
Acute Infection ◽  
Hydrophobic Membrane ◽  
Lethal Infection ◽  
Wide Range

ABSTRACT Pathogenic Leptospira species adapt to a wide range of environmental conditions during disease transmission and infection. While the proteome of in vitro cultivated Leptospira has been characterized in several studies to date, relatively little is known of the proteome as expressed by Leptospira during disease processes. Isolates of Leptospira obtained from patients suffering the severe pulmonary form of leptospirosis cause acute lethal infection in guinea pigs and chronic asymptomatic infection in rats. Recent studies have demonstrated that protein and lipopolysaccharide constituents of Leptospira recovered from acutely infected guinea pig tissue differ from that of Leptospira in chronically infected rat tissue and in vitro cultivated Leptospira (J. E. Nally, E. Chow, M. C. Fishbein, D. R. Blanco, and M. A. Lovett, Infect. Immun. 73:3251-3260, 2005). In the current study, the proteome of Leptospira expressed during disease processes was characterized relative to that of in vitro cultivated Leptospira (IVCL) after enrichment for hydrophobic membrane proteins with Triton X-114. Protein samples were separated by two-dimensional gel electrophoresis, and antigens expressed during infection were identified by immunoblotting with monospecific antiserum and convalescent rat serum in addition to mass spectrometry. Results suggest a significant increase in the expression of the outer membrane protein Loa22 during acute infection of guinea pigs relative to other outer membrane proteins, whose expression is generally diminished relative to expression in IVCL. Significant amounts of LipL32 are also expressed by Leptospira during acute infection of guinea pigs.

scholarly journals Computational identification of significant immunogenic epitopes of the putative outer membrane proteins from Mycobacterium tuberculosis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Shobana Sundar ◽  
Lokesh Thangamani ◽  
Shanmughavel Piramanayagam
Keyword(s):  
Membrane Proteins ◽  
B Cell ◽  
Outer Membrane ◽  
In Silico ◽  
Outer Membrane Proteins ◽  
Cell Binding ◽  
Immunogenic Peptides ◽  
Antigenic Properties ◽  
Mhc Alleles ◽  
Immunodominant Epitopes

AbstractNovel vaccines are required to effectively combat the epidemic spread of tuberculosis. Using in silico approaches, this study focuses on prediction of potential B cell and T cell binding immunogenic epitopes for 30 putative outer membrane proteins of Mtb. Among these, certain immunodominant epitopes of Rv0172, Rv0295c, Rv1006, Rv2264c, and Rv2525c were found, which are capable of binding B-cell and a maximum number of MHC alleles. The selected immunodominant epitopes were screened for their allergenic and antigenic properties, their percentage identity against the human proteome and their structural properties. Further, the binding efficacy of the immunodominant epitopes of Rv0295c and Rv1006 with HLA-DRB1*04:01 was analyzed using molecular docking and molecular dynamics studies. Hence, the in silico-derived immunogenic peptides (epitopes) could potentially be used for the design of subunit vaccines against tuberculosis.

Use of synthetic peptides to identify surface-exposed, linear B-cell epitopes within outer membrane protein F of Pseudomonas aeruginosa

1995 ◽  
Vol 31 (5) ◽  
pp. 279-286 ◽  
Author(s):  
Harry E. Gilleland ◽  
Eileen E. Hughes ◽  
Linda B. Gilleland ◽  
Janice M. Matthews-Greer ◽  
John Staczek
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Protein ◽  
B Cell ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Synthetic Peptides ◽  
B Cell Epitopes ◽  
Protein F ◽  
Outer Membrane Protein F ◽  
Linear B

scholarly journals Helicobacter pylori Physiology Predicted from Genomic Comparison of Two Strains

1999 ◽  
Vol 63 (3) ◽  
pp. 675-707 ◽  
Author(s):  
Peter Doig ◽  
Boudewijn L. de Jonge ◽  
Richard A. Alm ◽  
Eric D. Brown ◽  
Maria Uria-Nickelsen ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Sulfur Metabolism ◽  
Outer Membrane Proteins ◽  
Anaerobic Respiration ◽  
Phase Variation ◽  
Carbohydrate Utilization ◽  
Wide Range ◽  
H Pylori

SUMMARY Helicobacter pylori is a gram-negative bacteria which colonizes the gastric mucosa of humans and is implicated in a wide range of gastroduodenal diseases. This paper reviews the physiology of this bacterium as predicted from the sequenced genomes of two unrelated strains and reconciles these predictions with the literature. In general, the predicted capabilities are in good agreement with reported experimental observations. H. pylori is limited in carbohydrate utilization and will use amino acids, for which it has transporter systems, as sources of carbon. Energy can be generated by fermentation, and the bacterium possesses components necessary for both aerobic and anaerobic respiration. Sulfur metabolism is limited, whereas nitrogen metabolism is extensive. There is active uptake of DNA via transformation and ample restriction-modification activities. The cell contains numerous outer membrane proteins, some of which are porins or involved in iron uptake. Some of these outer membrane proteins and the lipopolysaccharide may be regulated by a slipped-strand repair mechanism which probably results in phase variation and plays a role in colonization. In contrast to a commonly held belief that H. pylori is a very diverse species, few differences were predicted in the physiology of these two unrelated strains, indicating that host and environmental factors probably play a significant role in the outocme of H. pylori-related disease.

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