scholarly journals Intestinal Protozoan Parasitosis in 4- and 11-year-old Children in Cunduana Community, Riobamba Canton, Chimborazo, June 2019 June

2021 ◽  
Author(s):  
Silvia Marcela Inca Martínez ◽  
Martha Cecilia Bonilla Caicedo ◽  
Silvana Paola Ocaña Coello
Keyword(s):  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Intellectual Development ◽  
Public Health Problem ◽  
Oral Route ◽  
School Age Children ◽  
Intestinal Protozoan ◽  
Intestinal Protozoa ◽  
Intestinal Parasitosis ◽  
Physiological Serum

Introduction: Parasitosis is a public health problem that affects millions of people, school-age children are more vulnerable, affects their physical and intellectual development, are transmitted by fecal-oral route, and is associated with poor hygienic and sanitary conditions. In Ecuador they constitute the second reason for medical consultation. Objective: To contribute to the epidemiological knowledge of intestinal parasitosis by protozoa in our environment. Methodology: An experimental descriptive study, with a universe of 80 children, obtaining 55 samples, a direct examination was carried out in preparations with physiological serum and lugol, identifying the parasites by their microscopic morphology. Results: The prevalence of intestinal parasitosis by protozoa was 78.18%, while for the parasites Endolimax nana 30.9% Entamoeba histolytica, 34.5% and Giardia lamblia 14.5%. 40%  of the participants were monoparasitized and 38.2% had polyparasitosis. Conclusion: High prevalence of parasitosis by intestinal protozoa was determined in the children of the Unit with 78.18%. The most prevalent ameboid protozoan was E. nana, followed by the pathogenic protozoan E. histolytica, while G. lamblia was flagellated protozoan identified. The lack of adequate sanitary infrastructure, habits of hygiene, environmental pollution, influence the prevalence of intestinal parasitosis. Keywords: protozoan infections, intestinal diseases, child. RESUMEN Introducción: Parasitosis es un problema de salud pública que afecta a millones de personas, los niños en edad escolar son más vulnerables, afecta su desarrollo físico e intelectual, se transmiten por vía fecal-oral, y está asociada a deficientes condiciones higiénicas y sanitarias. En Ecuador constituyen el segundo motivo de consulta médica. Objetivo: contribuir al conocimiento epidemiológico de las parasitosis intestinales por protozoos en nuestro medio. Metodología: estudio descriptivo experimental, con un universo de 80 niños y niñas, obteniendo 55 muestras, se realizó examen directo en preparaciones con suero fisiológico y lugol, identificando los parásitos por su morfología microscópica. Resultados: La prevalencia de parasitosis intestinal por protozoos fue de 78,18%, mientras que para los parásitos Endolimax nana 30,9%, Entamoeba histolytica 34,5% y Giardia lamblia 14,5%. El 40% de los participantes fue monoparasitado y el 38,2% presentó poliparasitosis. Conclusiones: Se determinó alta prevalencia de parasitosis por protozoos intestinales en los niños y niñas de la Unidad con un 78,18%. El protozoo ameboideo de mayor prevalencia fue E. nana, seguido del protozoo patógeno E. histolytica, mientras que G. lambia fue protozoo flagelado identificado. La falta de una adecuada infraestructura sanitaria, hábitos de higiene, contaminación ambiental, influyen en la prevalencia de la parasitosis intestinal. Palabras clave: infecciones por protozoos, parasitosis intestinales, niños.

scholarly journals Prevalence Des Infections Parasitaires Dues Aux Protozoaires Identifies Au Laboratoire National De Biologie Clinique Et De Sante Publique, Bangui Republique Centrafricaine

2021 ◽  
Vol 17 (21) ◽  
pp. 115
Author(s):  
Ernest Lango-Yaya ◽  
Donatien Clotaire Rafai ◽  
Tatiana Ngalema ◽  
Freddy Marcelin Agboko ◽  
Romaric Lebon Bondom ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Santé Publique ◽  
Public Health Problem ◽  
Giardia Intestinalis ◽  
Age Group ◽  
Intestinal Protozoa ◽  
Intestinal Parasitosis ◽  
Fresh State ◽  
The World ◽  
Sante Publique

Les Protozoaires sont des parasites de petite taille, de forme diverses parmi lesquels on distingue les Protozoaires intestinaux (Amibes, Flagellés, Coccidies, Ciliés et Microsporidies) qui sont les plus répandus. Selon l’OMS, les parasitoses intestinales constituent un problème de santé publique dans le monde en général et en Afrique en particulier. Les formes graves sont généralement provoquées par certains protozoaires intestinaux à savoir Entamoeba histolytica, Giardia intestinalis et Trichomonas intestinalis qui jouent un rôle dans la survenue du syndrome dysentérique. C’est dans ce contexte que cette étude est menée dans l’objectif est d’évaluer la prévalence des Protozoaires digestifs selon leur degré de pathogénicité chez les patients. La mise en évidence des parasites a été effectuée par la technique de Formolether et de l’examen à l’état frais. Cette étude a montré que sur 11500 patients, 3922 soit 34,1% étaient parasités. Parmi les patients parasités le sexe féminin était prédominant avec un pourcentage de 57,51%. La tranche d’âge la plus touchée est celle comprises entre 20 à 29 ans (39,4%), les enfants de moins de 1 an étaient moins touchés (1,2%). Le parasite le plus représentatif est le kyste d’Entamoeba histolytica 30,40% suivi de Trichomonas intestinalis 1,4%. L’amibiase, la giardiose et la trichomonose restent les protozooses les plus pathogènes associées à l’hygiène défectueuse et du péril fécal. Protozoa are parasites of small size, of various shapes, among which we distinguish the intestinal Protozoa (Amoeba, Flagellates, Coccidia, Ciliates and Microsporidia) which are the most widespread. According to WHO, intestinal parasitosis is a public health problem in the world in general and in Africa in particular. The severe forms are generally caused by certain intestinal protozoa namely Entamoeba histolytica, Giardia intestinalis and Trichomonas intestinalis which play a role in the occurrence of dysenteric syndrome. It is in this context that this study is being carried out with the objective of assessing the prevalence of digestive protozoa according to their degree of pathogenicity in patients. The detection of the parasites was carried out by the formalin-ether technique and the examination in the fresh state. This study showed that out of 11,500 patients, 3,922 or 34.1% were parasitized. Among the parasitized patients the female sex was predominant with a percentage of 57.51%. The most affected age group is between 20 to 29 years old (39.4%), children under 1 year old were less affected (1.2%). The most representative parasite is the Entamoeba histolytica cyst 30.40% followed by Trichomonas intestinalis 1.4%. Amebiasis, giardiasis and trichomoniasis remain the most pathogenic protozoa associated with poor hygiene and faecal hazard.

scholarly journals Evaluation of Intestinal Protozoan Morphology in Human Fecal Specimens Preserved in EcoFix: Comparison of Wheatley’s Trichrome Stain and EcoStain

1998 ◽  
Vol 36 (7) ◽  
pp. 1974-1976 ◽  
Author(s):  
Lynne S. Garcia ◽  
Robyn Y. Shimizu
Keyword(s):  
Polyvinyl Alcohol ◽  
Giardia Lamblia ◽  
Blastocystis Hominis ◽  
Intestinal Protozoan ◽  
Entamoeba Dispar ◽  
Intestinal Protozoa ◽  
Mercury Compounds ◽  
Dientamoeba Fragilis ◽  
Color Differences

As a result of disposal problems related to the use of mercury compounds, many laboratories have switched from mercuric chloride-based Schaudinn’s and polyvinyl alcohol (PVA) stool preservatives to other, non-mercury-based preservatives. A comparison of organism recoveries and morphologies of the intestinal protozoa was undertaken with PVA containing the EcoFix zinc-based Schaudinn’s preservative (Meridian Diagnostics, Inc.); both Wheatley’s modification of Gomori’s trichrome stain (WT) and EcoStain (ES) were used to stain 51 human fecal specimens. Morphology, clarity of nuclear and cytoplasmic detail, overall color differences, and the ease or difficulty in detecting intestinal protozoa in fecal debris were assessed for the two permanent stained smears. Overall, organism morphology of the intestinal protozoa stained with WT and that of protozoa stained with ES were not equal in nuclear and cytoplasmic detail or range of color. However, the same organisms were identified in stained fecal smears with either WT or ES, with the exception of situations in which organism numbers were characterized as rare. Included were 67 protozoan challenges (number of organisms): Entamoeba histolytica-Entamoeba dispar (5), Entamoeba coli (9),Entamoeba hartmanni (6), Endolimax nana (12),Iodamoeba bütschlii (8), Blastocystis hominis (19), Giardia lamblia (6), Dientamoeba fragilis (2), yeast (2), and leukocytes (2). Five specimens were negative for parasites but contained fecal debris that was compared for morphologic detail and color range. The ES produces a more gray-green monotone with very little pink or red tone; contrast among the various colors is less than that seen with WT. Stain intensity for all organisms was acceptable, and there were no problems with stain deposition. The quality of the protozoan morphology with ES was often comparable to that with WT (36 of 67 [53.7%]) and, in some cases, better (24 of 67 [35.8%]). Organisms on the WT-stained smear exhibited better morphology in a few instances (4 of 67 [6%]), and in three instances, there were discrepant organism numbers.

Prevalence and pre-disposing factors associated to gastro intestinal protozoan infections among diarrheal children under 5 years in kisii county, kenya.

2019 ◽  
Author(s):  
Caleb Okeri Ondara ◽  
Benson Omweri Nyachong’i ◽  
Vincent Obino Orucho
Keyword(s):  
Giardia Lamblia ◽  
Health Issues ◽  
Diarrheal Diseases ◽  
Intestinal Protozoan ◽  
Intestinal Protozoa ◽  
Mortality And Morbidity ◽  
Protozoan Infection ◽  
High Prevalence ◽  
Protozoan Infections ◽  
Children Under 5

Abstract ABSTRACT. Objectives: Mortality and morbidity due to diarrheal diseases among children below the age of five has been increasing especially in the developing countries. This infection has not got attention like other health issues at national or regional levels. Documenting the risk prevalence and factors that influence the occurrence of the diarrheal diseases help to inform the and to develop preventive strategies for the county of study and the country at large. Results: There was high prevalence of gastro intestinal protozoan infection with 34(28.3%) children infected with either Entamoeba histolytica, Giardia lamblia or both (co infections). Female children were 2.1 times less likely to be infected with gastro intestinal protozoa than their male counterparts, though not statistic ally different (P=0.392). Keywords: Gastro intestinal protozoans, diarrhea, children under 5 years

scholarly journals Virtual Screening of FDA-Approved Drugs against Triose Phosphate Isomerase from Entamoeba histolytica and Giardia lamblia Identifies Inhibitors of Their Trophozoite Growth Phase

2021 ◽  
Vol 22 (11) ◽  
pp. 5943
Author(s):  
Alfredo Juárez-Saldivar ◽  
Elizabeth Barbosa-Cabrera ◽  
Edgar E. Lara-Ramírez ◽  
Alma D. Paz-González ◽  
Ana V. Martínez-Vázquez ◽  
...  
Keyword(s):  
Virtual Screening ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Triosephosphate Isomerase ◽  
Glycolytic Enzyme ◽  
Public Health Issue ◽  
Intestinal Protozoan ◽  
Approved Drugs ◽  
Fda Approved Drugs

Infectious diseases caused by intestinal protozoan, such as Entamoeba histolytica (E. histolytica) and Giardia lamblia (G. lamblia) are a worldwide public health issue. They affect more than 70 million people every year. They colonize intestines causing primarily diarrhea; nevertheless, these infections can lead to more serious complications. The treatment of choice, metronidazole, is in doubt due to adverse effects and resistance. Therefore, there is a need for new compounds against these parasites. In this work, a structure-based virtual screening of FDA-approved drugs was performed to identify compounds with antiprotozoal activity. The glycolytic enzyme triosephosphate isomerase, present in both E. histolytica and G. lamblia, was used as the drug target. The compounds with the best average docking score on both structures were selected for the in vitro evaluation. Three compounds, chlorhexidine, tolcapone, and imatinib, were capable of inhibit growth on G. lamblia trophozoites (0.05–4.935 μg/mL), while folic acid showed activity against E. histolytica (0.186 μg/mL) and G. lamblia (5.342 μg/mL).

scholarly journals Prevalence of Intestinal Parasitosis in Children of a Primary School in Pedro Juan Caballero - PY

2020 ◽  
Vol 5 (10) ◽  
pp. 424-426
Author(s):  
Kayque Wellek Delgado do Amaral ◽  
Cristiane Venturini Garlet ◽  
Daniel Contreira Júnior ◽  
Marcus Tadeu Fernandes Gomes da Silva ◽  
Laura Simões Cogorno ◽  
...  
Keyword(s):  
Public Health ◽  
Giardia Lamblia ◽  
Preventive Measures ◽  
Intestinal Parasites ◽  
Public Health Problem ◽  
Important Public Health Problem ◽  
Sedimentation Technique ◽  
Intestinal Parasitosis ◽  
Stool Samples ◽  
Sanitary Conditions

Introduction: Parasitosis is a type of endoparasitism, which is directly related to sanitary conditions and is an important public health problem in developing countries. Objective: To determine the prevalence of intestinal parasites in children attending a primary public school in Pedro Juan Caballero - PY. Materials and Methods: 85 (eighty-five) stool samples were analyzed using the centrifugal sedimentation technique. Results: Cysts and/or parasite eggs were observed in the stool samples of 67 (78.8%) children. Giardia lamblia cysts were those that showed the highest incidence in the children. Conclusion: The high Giardia lamblia index demonstrates the lack of access to drinking water for consumption, inadequate sanitation and the absence of a public health network. This study highlights the importance of encouraging the use of serial analysis of stool, strengthening preventive measures for parasitosis, developing protocols for their approach, and designing a strategy that includes the preschool population in the programs that periodically evaluate the development of children.

scholarly journals Comparative Pathobiology of the Intestinal Protozoan Parasites Giardia lamblia, Entamoeba histolytica, and Cryptosporidium parvum

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 116 ◽  
Author(s):  
Hemphill ◽  
Müller ◽  
Müller
Keyword(s):  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Giardia Lamblia ◽  
Intestinal Tract ◽  
Protozoan Parasites ◽  
Intestinal Protozoan ◽  
Colon Wall ◽  
Causative Agents ◽  
Life Threatening ◽  
Human Intestinal Tract

Protozoan parasites can infect the human intestinal tract causing serious diseases. In the following article, we focused on the three most prominent intestinal protozoan pathogens, namely, Giardia lamblia, Entamoeba histolytica, and Cryptosporidium parvum. Both C. parvum and G. lamblia colonize the duodenum, jejunum, and ileum and are the most common causative agents of persistent diarrhea (i.e., cryptosporidiosis and giardiasis). Entamoeba histolytica colonizes the colon and, unlike the two former pathogens, may invade the colon wall and disseminate to other organs, mainly the liver, thereby causing life-threatening amebiasis. Here, we present condensed information concerning the pathobiology of these three diseases.

scholarly journals An analytical study of intestinal parasitosis in children

2017 ◽  
Vol 4 (7) ◽  
pp. 2543 ◽  
Author(s):  
Rituparna Bhattacharya ◽  
Kanai Lal Barik ◽  
Promukh Bhattacharya ◽  
Uttam Kumar Paul
Keyword(s):  
Pediatric Population ◽  
Public Health Problem ◽  
Mass Scale ◽  
School Age Children ◽  
Deficiency Anemia ◽  
Important Public Health Problem ◽  
Positive Stool ◽  
Intestinal Parasitosis ◽  
Ancylostoma Duodenale ◽  
The Government

Background: Intestinal parasitosis (IP) is an important public health problem worldwide, most commonly seen in school age children and lead to nutritional deficiency, anemia and impaired cognition. Dearth of data regarding parasitic prevalence in the pediatric population triggered such a retrospective study planned by the Departments of Pediatrics and Microbiology, of two Medical Colleges whereby all the symptomatic children (>1 year and <12 years, divided into less than 5 year and 5-12 year groups) were examined for IP.Methods: Total 1000 parasite positive stool samples were collected for a period of two years and included for the study. Routine macroscopic and microscopic (saline and Lugol’s iodine wet mounts) examinations were carried out for the presence of ova, cysts and parasites.Results: Out of the 1000 positive samples, Enterobius vermicularis was predominant with 287 (28.7%) of all cases. The next in order were Giardia lamblia 243 (24.3%), Entamoeba histolytica 219 (21.9%), Ascaris lumbricoides 143 (14.3%), Ancylostoma duodenale 39 (3.9%), Taenia 27 (2.7%) and others 42 (4.2%). The distribution of parasites were heavier among the 5 to 12 year group (74.6%) than the below 5 year one (25.4%), with single parasitic isolate. Lack of hygienic practices like open field defecation, faecal contamination of water and improper hand washing aggravate infestation.Conclusions: Thus our study revealed that steps are to be taken by both the government and society to promote healthcare awareness in mothers, and mass scale deworming through school campaigns in order to lower the parasitic burden in children. 

scholarly journals GAMBARAN INFEKSI PROTOZOA INTESTINAL PADA ANAK BINAAN RUMAH SINGGAH AMANAH KOTA PADANG

2015 ◽  
Vol 34 (1) ◽  
pp. 60
Author(s):  
Nurhayati Nurhayati
Keyword(s):  
Public Health ◽  
Public Health Problem ◽  
Stool Examination ◽  
Intestinal Protozoan ◽  
Intestinal Protozoa ◽  
Fecal Examination ◽  
Examination Method ◽  
Protozoan Infection ◽  
Protozoan Pathogen ◽  
Frequent Infection

AbstrakInfeksi protozoa intestinal masih merupakan masalah kesehatan masyarakat di negara tropis dan negara berkembang. Yang termasuk ke dalam protozoa intestinal patogen di antaranya adalah G. lamblia dan E. histolitika.Telah dilakukan penelitian terhadap anak binaan Rumah Singgah “Amanah”, Kelurahan Rimbo Kaluang, Kecamatan Padang Barat, Kota Padang. Pemeriksaan tinja dilakukan terhadap 66 anak dengan metode langsung menggunakan eosin dan lugol. Tujuan penelitian ini adalah untuk mengetahui gambaran infeksi protozoa intestinal pada anak binaan rumah singgah Amanah.Telah dilakukan penelitian di Rumah Singgah “Amanah”, Kelurahan Rimbo Kaluang, Kecamatan Padang Barat, Kota Padang, terhadap anak biaHasil penelitian ini menunjukkan bahwa anak-anak yang terinfeksi protozoa intestinal sebesar 40,91%. Berdasarkan jenis spesies, distribusi frekuensi terbanyak yang menginfeksi anak adalah G. lamblia yaitu 37,88%, sedangkan infeksi oleh E. histolitika adalah 3,03%. Frekuensi infeksi G. Lamblia lebih tinggi pada umur < 10 tahun yaitu 27,27%, tetapi pada infeksi E. histolitika terlihat tidak ada perbedaan. Distribusi infeksi berdasarkan jenis kelamin hampir sama pada G. lamblia maupun E. histolitika. Berdasarkan pekerjaan, lebih separuh anak binaan yang terinfeksi protozoa intestinal bekerja sebagai penjaja makanan.Kata kunci: Protozoa intestinal, G. lamblia, E. histolitikaAbstractPrevalence of intestinal protozoan infection in Rumah Singgah Amanah, Kota Padang. Intestinal protozoan infection is still a public health problem in tropical countries and developing countries. The intestinal protozoan pathogen of which is G. lamblia and E.histolitika.This research is descriptif study and it was conducted in Rumah Singgah Amanah, Kelurahan Rimbo Kaluang, Kecamatan Padang Barat, Kota Padang. Stool examination has been carried out to 66 children by direct fecal examination method using eosin and Lugol. The purpose of this research is to know the description of intestinal protozoan in fection in Rumah Singgah Amanah.Prevalence of intestinal protozoa infection was 40,91%, the highest frequent infection was G. lamblia which was 37.88%, E. histolitika was 3.03%. FrequencyARTIKEL PENELITIAN61of G. lamblia infection was higher in age <10 years is 27.27%. There was no different in age in E.histolytica infection. There was no different in sex in both infection. Half of children with intestinal protozoa infection were food seller.Key words : Intestinal Protozoa, G. lamblia, E. histolitika

scholarly journals Study the infection with intestinal protozoa Entamoeba histolytica and Giardia lamblia among Patients who attending Bint Al- Huda for maternity and children hospital and Al hussin hospital in Nassriyia city in Thi-Qar province

2015 ◽  
Vol 12 (3) ◽  
pp. 468-473
Author(s):  
Baghdad Science Journal
Keyword(s):  
Drinking Water ◽  
Family Member ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Parasitic Infection ◽  
Family Members ◽  
Age Group ◽  
Prevalence Rates ◽  
Intestinal Protozoa ◽  
One Year

The aims of this study the infection with parasites Entamoeba histolytica and Giardia lamblia and prevalence rates and their relationship with gende , age group , the number of family members and source of drinking water among the patients at attending the Bint Al Huda and Al- Hussin Teaching hospital in Nassriyia city in Thi- Qar province , it was noticed that the percentage of parasitic infection at females was 38.55% and at males was 61.45% and the percentage of parasitic infection with Entamoeba histolytica 97% was higher than the percentage of parasitic infection with Giardia lamblia 3%,and the reduction of percentage of infection is associated with increasing of the number of family member ,the percentage of infection was 49% at the age group less than one year while it was 4% at the age group more than 15 year ,and the percentage of infection was increasing with number of family members .It was noticed that the higher percentage of infection was (45%) at families with members more than 15 person

scholarly journals Spatial Distribution of Common Pathogenic Human Intestinal Protozoa in Iran: A Systematic Review

2021 ◽  
Author(s):  
Mehdi MOHEBALI ◽  
Hossein KESHAVARZ ◽  
Mohammad Javad ABBASZADEH AFSHAR ◽  
Ahmad Ali HANAFI-BOJD ◽  
Gholamreza HASSANPOUR
Keyword(s):  
Public Health ◽  
Spatial Distribution ◽  
General Population ◽  
Public Health Problem ◽  
Analysis Model ◽  
Intestinal Protozoan ◽  
Public Health Importance ◽  
Intestinal Protozoa ◽  
Cryptosporidium Spp ◽  
Protozoan Infections

Background: Pathogenic intestinal protozoa are considered as a serious public health problem in developing countries. This study aimed to elucidate the overall prevalence and spatial distribution of three common human pathogenic intestinal protozoan infections in Iran. Methods: Six English and Persian databases were explored for published papers on the prevalence of Entamoeba histolytica/dispar, Giardia lamblia, and Cryptosporidium spp. in the general population of Iran from 2000 to 2015. All eligible data were collected using a pre-designed data extraction form, and the overall prevalence was estimated using a random-effects meta-analysis model. We used ArcMap for mapping the prevalence of the studied protozoa and clustering analysis. Results: Altogether, 118 eligible papers from 24 provinces of Iran were included and analyzed. The weighted prevalence of E. histolytica/dispar, G. lamblia, and Cryptosporidium spp. infection among Iranian general population were calculated 1.3% (95% CI 1.1-1.5%), 10.6% (95% CI 9.6-11.5%) and 2% (95% CI 1.5-2.5%), respectively. Conclusion: Our findings indicated human intestinal protozoan infections caused by E. histolytica/dispar, G. lamblia, and Cryptosporidium spp. have still public health importance in some parts of Iran.  

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