Evaluation of the Larvicidal Potential of the Essential Oil Pogostemon cablin (Blanco) Benth in the Control of Aedes aegypti

The objective of this work was to collect information on the chemical constituents that demonstrate the larvicidal activity against Aedes aegypti, as well as the antioxidant, microbiological, and cytotoxicity potential of the essential oil of Pogostemon cablin leaves. The chemical characterization was performed by gas chromatography coupled to mass spectrometer (GC-MS). The larvicidal activity was performed according to the protocol of the World Health Organization. The antioxidant activity was evaluated through the sequestering capacity of 2,2-diphenyl-1-picryl-hydrazine (DPPH). As for the microbiological evaluation, the microdilution technique was used, according to the protocol of the Clinical and Laboratory Standards Institute. The cytotoxic activity was evaluated against the larvae of Artemia salina. The species P. cablin presented the following compounds: Patchouli alcohol (33.25%), Seyshellene (6.12%), α-bulnesene (4.11%), Pogostol (6.33%), and Norpatchoulenol (5.72%), which was in synergy with the other substances may significantly potentiate the larvicidal action of the species with the LC50 of 28.43 μg·mL−1. There was no antioxidant activity, however, it presented antimicrobial activity against all bacteria tested with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of 62.5 μg·mL−1. The species demonstrated significant toxic action with LC50 of 24.25 μg·mL−1. Therefore, the P. cablin species showed significant larvicidal potential, antimicrobial activity, the absence of antioxidant action, and high toxicity.

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Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

10.1101/595900 ◽

2019 ◽

Cited By ~ 1

Author(s):

Renata do Socorro Barbosa Chaves ◽

Rosany Lopes Martins ◽

Alex Bruno Lobato Rodrigues ◽

Érica de Menezes Rabelo ◽

Ana Luzia Ferreira Farias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Artemia Salina ◽

High Cytotoxic Activity ◽

Origanum Majorana ◽

Main Component

ABSTRACTThis study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

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Chemical Characterization, Antioxidant, Cytotoxic and Microbiological Activities of the Essential Oil of Leaf of Tithonia Diversifolia (Hemsl) A. Gray (Asteraceae)

Pharmaceuticals ◽

10.3390/ph12010034 ◽

2019 ◽

Vol 12 (1) ◽

pp. 34 ◽

Cited By ~ 2

Author(s):

Ana Ferreira Farias ◽

Alex Lobato Rodrigues ◽

Rosany Lopes Martins ◽

Érica de Menezes Rabelo ◽

Carlos Ferreira Farias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Cytotoxic Activity ◽

Chemical Constituents ◽

Larval Mortality ◽

Chemical Characterization ◽

Artemia Salina ◽

Antioxidant Potential ◽

Tithonia Diversifolia ◽

Bacterial Strains

The present study aimed to evaluate the chemical composition, antioxidant potential, and the cytotoxic and antimicrobial activity of the essential oil of the plant species Tithonia diversifolia (Hemsl) A. Gray. The essential oil obtained was used to identify the chemical compounds present through the techniques of GC-MS and NMR. The antioxidant potential was calculated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl. For cytotoxic activity, the larval mortality of Artemia salina was evaluated. The main chemical constituents identified are αpinene (9.9%), Limonene (5.40%), (Z)-β-ocimene (4.02%), p-cymen-8-ol (3.0%), Piperitone (11.72%), (E)-nerolidol (3.78%) and Spathulenol (10.8%). In the evaluation of the antimicrobial activity, bacterial strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used. The results showed that the bacterium E. coli were more susceptible to the presence of the essential oil, presenting minimal inhibitory concentration at the concentrations that were exposed. The essential oil presented antioxidant activity of 54.6% at the concentration of 5 mg·mL−1 and provided a CI50 of 4.30. It was observed that the essential oil of this species was highly toxic against A. salina lavas, as its cytotoxic activity showed an LC50 of 3.11. Thus, it is concluded that T. diversifolia oils are effective in inhibiting bacterial growth and reducing oxidative stress.

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Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina

Eclética Química Journal ◽

10.26850/1678-4618eqj.v44.4.2019.p85-95 ◽

2019 ◽

Vol 44 (4) ◽

pp. 85 ◽

Cited By ~ 1

Author(s):

Paulo Roberto Barros Gomes ◽

Marlucy Bezerra Oliveira ◽

Dionney Andrade De Sousa ◽

Jeremias Caetano Da Silva ◽

Romer Pessôa Fernandes ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Biological Activities ◽

Biomphalaria Glabrata ◽

Artemia Salina ◽

World Health ◽

Lethal Concentration ◽

Major Constituent ◽

Citrus Limon

In this present work, we tested the larvicidal activity, molluscicide and toxicity of the oil extracted from Citrus limon peels, respectively against third stage larvae of Aedes aegypti, snail Biomphalaria glabrata, and Artemia salina. For this, we extract the essential oil by hydrodistillation. Then, we identified and quantified the components by gas chromatography coupled to mass spectrometry (GC-MS). We tested the larvicidal and molluscicidal activity, respectively, using the method adopted by the Brazilian Ministry of Health and the World Health Organization. We calculated the lethal concentration (LC50) from the Probit method for the three biological activities with 95 %. The results of the chromatographic analysis showed that the oil has 58.81% of Limonene (major constituent) and 0.11% α-Mulene (minority component). The essential oil presented lethal concentration (LC50) for larvicidal activity, molluscicide and toxicity, respectively at 15.48, 13.05 and 743.35 mg∙L-1. Therefore, the essential oil is active against larvae of A. aegypti and snail B. glabrata and non-toxic against larvae of A. salina.

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Larvicidal Evaluation against Aedes aegypti and Antioxidant and Cytotoxic Potential of the Essential Oil of Tridax procumbens L. Leaves

The Scientific World JOURNAL ◽

10.1155/2021/2172919 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Lethicia B. Brandão ◽

Lizandra L. Santos ◽

Rosany L. Martins ◽

Alex B. L. Rodrigues ◽

Erica de M. Rabelo ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Aedes Aegypti ◽

Cytotoxic Activity ◽

Artemia Salina ◽

Stage Of Development ◽

Tridax Procumbens ◽

Third Stage ◽

Low Toxicity ◽

Larvicidal Bioassay

The present study evaluated the antioxidant, cytotoxic, and larvicidal potential of the essential oil of Tridax procumbens leaves, as well as identified the compounds present in the essential oil. The antioxidant activity was evaluated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl radical, the cytotoxic activity was evaluated using Artemia salina, the larvicidal bioassay was performed with larvae in the third stage of development of the Aedes aegypti mosquito, and the identification of the metabolites was performed by gas chromatography coupled to the mass spectrometer (GC-MS). The phytochemical oil analysis showed the presence of 20 compounds, with thymol and γ-terpinene being the main ones. It presented antioxidant activity with an IC50 of 194.51 μg mL−1, demonstrating antioxidant activity in the highest concentrations tested. It presented low cytotoxic activity against A. salina, with an LC50 of 1238.67 μg mL−1, demonstrating atoxicity in the concentrations tested. The essential oil presented good larvicidal activity when compared to the literature, with an LC50 = 79.0 μg mL−1 in 24 hours and LC50 of 69.15 μg mL−1 in 48 hours. In this way, it was possible to identify that the essential oil of the leaves of T. procumbens presented potential for the development of a natural larvicide, as well as antioxidant activity satisfactory to the radical DPPH and low toxicity to A. salina.

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Chemical Composition and Larvicidal Activity of Eugenia triquetra Essential Oil from Venezuelan Andes

Natural Product Communications ◽

10.1177/1934578x1000500633 ◽

2010 ◽

Vol 5 (6) ◽

pp. 1934578X1000500 ◽

Cited By ~ 1

Author(s):

Flor D. Mora ◽

Jorge L. Avila ◽

Luis B. Rojas ◽

Rosslyn Ramírez ◽

Alfredo Usubillaga ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Chemical Constituents ◽

Ms Analysis ◽

Third Instar Larvae

The chemical constituents of the essential oil obtained by hydrodistillation of the leaves of Eugenia triquetra O. Berg, collected in Táchira State, Venezuela, were identified by GC-MS analysis. Twenty-six components, which made up 88.5% of the oil, were identified. The major constituents were linalool (17.5%), limonene (16.9%), α-pinene (11.6%), β-pinene (8.7%), and p-cymene (3.7%). The essential oil was tested against third-instar larvae of Aedes aegypti, showing a LC50 value of 64.8 ± 5.6 ppm.

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Physicochemical properties, toxicity and larvicidal activity of the essential oil of Cymbopogon winterianus in front of Aedes aegypti

Ciência e Natura ◽

10.5902/2179460x44044 ◽

2020 ◽

Vol 42 ◽

pp. e39

Author(s):

Gabriela Morais da Costa ◽

Maria Conceição Carneiro dos Santos ◽

Gustavo Oliveira Everton ◽

Paulina De Cássia Duarte de Sousa ◽

Maurício Eduardo Salgado Rangel

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Physicochemical Properties ◽

Larvicidal Activity ◽

Artemia Salina ◽

Toxicity Assay ◽

Toxicity Effect ◽

Highly Active ◽

Cymbopogon Winterianus ◽

Current Context

Due to the increasing rate of morbidity and mortality caused by vector diseases in the current context, especially by Aedes aegypti, substances of plants have been studied as alternatives to chemical insecticides, among them, the essential oil (EO) of the species Cymbopogon winterianus. Thus, this study evaluated the larvicidal activity of the EO of C. winterianus against the A. aegypti larvae. EO was extracted through the hydro-distillation technique and physicochemical properties were determined. To evaluate larvicidal activity, tests were performed with larvae in the third instar at the final concentrations of 19.54; 26.50; 55.59; 138.98; 208.47 and 277.97 mg L-1 of C. winterianus EO. In addition, Artemia salina Leach bioassay was used to verify toxicity effect. EO obtained presented satisfactory results in 2.64%. In the larvicidal assay, 100% mortality of larvae was observed after 24 hours at concentrations of 208.47 mg L-1 and 277.97 mg L-1 of the EO, showed effective in the other concentrations and with LC50 of 46.18 mg L-1, considered highly active. In the toxicity assay, the EO presented LC50 at 532.34 mg L-1, showed considered nontoxic. These results reinforce the use of EO front A. aegypt larvae control.

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Antimicrobial activity of the essential oil from Bixa orellana L.

Research Society and Development ◽

10.33448/rsd-v9i10.8447 ◽

2020 ◽

Vol 9 (10) ◽

pp. e8129108447

Author(s):

Yuri Nascimento Fróes ◽

Ana Patrícia Matos Pereira ◽

Paulo Victor Serra Rosa ◽

Laiane Araujo da Silva Souto ◽

Mariana Oliveira Arruda ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Total Phenolics ◽

Chemical Characterization ◽

Artemia Salina ◽

Bixa Orellana ◽

Toxicity Assay ◽

Natural Substances ◽

Application Potential ◽

And Staphylococcus Aureus

The application of natural substances with bactericidal action is the target of research aimed at a more sustainable production, implying in various sources of substances for new therapeutic formulations. This study presents the chemical characterization, toxicity and antimicrobial activity of essential oil (EO) of Bixa orellana Labill leaves. EO was extracted by hydrodistillation at 100°C for 3h. Physicochemical parameters were determined and chemical characterization used Gas chromatography coupled with Mass Spectrometry (GC/MS). The toxicity assay was performed through the bioassay with Artemia salina Leach. The methods of Disc Diffusion and Dilution in Broth in front of the bacteria Escherichia coli and Staphylococcus aureus were used to evaluate the antimicrobial activity. LC50 in the toxicity assay was verified in 355 mg L-1 and was classified as nontoxic. The OE presented antimicrobial activity against the microorganisms tested and totaled 320 mg EAT g-1 of total phenolics. The results obtained highlight the importance of pointing out this product as a therapeutic alternative, encouraging its application potential.

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Antitumor and Aedes aegypti Larvicidal Activities of Essential Oils From Piper klotzschianum, P. hispidum, and P. arboreum

Natural Product Communications ◽

10.1177/1934578x19863932 ◽

2019 ◽

Vol 14 (7) ◽

pp. 1934578X1986393 ◽

Cited By ~ 1

Author(s):

Rafaely N. Lima ◽

Adauto S. Ribeiro ◽

Gilvandete M.P. Santiago ◽

Cinara O. d´S. Costa ◽

Milena B. Soares ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Cytotoxic Activity ◽

Larvicidal Activity ◽

Chemical Constituents ◽

Promyelocytic Leukemia ◽

Germacrene D ◽

Larvicidal Activities

The chemical constituents of essential oils extracted by hydrodistillation from Piper klotzschianum, P. hispidum, and P. arboreum, collected from the remaining Atlantic forest in Sergipe State, were characterized and submitted to cytotoxic and larvicidal activity assays. The major constituents identified (stems, fresh, and dried leaves) were ( E)-caryophyllene (16.8%) and bicyclogermacrene (21.6%). Germacrene D (22.8%) was identified only in P. klotzschianum and P. hispidum. A high percentage of β-pinene (27.2%) and α-pinene (7.2%) were present in P. klotzschianum stems and δ-3-carene in the fresh and dried leaves of P. hispidum (17.4% and 19.1%, respectively). Piper klotzschianum showed the best cytotoxic activity, inhibiting human hepatocellular carcinoma (27.3 µg/mL), human promyelocytic leukemia (33.8 µg/mL), and murine melanoma (37.9 µg/mL) cell lines. Larvicidal activity was also tested and the essential oil from the dried leaves of P. klotzschianum was the most potent against Aedes aegypti larvae, with an LC50 value of 122.4 µg/mL.

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