Antimicrobial activity of the essential oil from Bixa orellana L.

The application of natural substances with bactericidal action is the target of research aimed at a more sustainable production, implying in various sources of substances for new therapeutic formulations. This study presents the chemical characterization, toxicity and antimicrobial activity of essential oil (EO) of Bixa orellana Labill leaves. EO was extracted by hydrodistillation at 100°C for 3h. Physicochemical parameters were determined and chemical characterization used Gas chromatography coupled with Mass Spectrometry (GC/MS). The toxicity assay was performed through the bioassay with Artemia salina Leach. The methods of Disc Diffusion and Dilution in Broth in front of the bacteria Escherichia coli and Staphylococcus aureus were used to evaluate the antimicrobial activity. LC50 in the toxicity assay was verified in 355 mg L-1 and was classified as nontoxic. The OE presented antimicrobial activity against the microorganisms tested and totaled 320 mg EAT g-1 of total phenolics. The results obtained highlight the importance of pointing out this product as a therapeutic alternative, encouraging its application potential.

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Chemical Characterization, Antioxidant, Cytotoxic and Microbiological Activities of the Essential Oil of Leaf of Tithonia Diversifolia (Hemsl) A. Gray (Asteraceae)

Pharmaceuticals ◽

10.3390/ph12010034 ◽

2019 ◽

Vol 12 (1) ◽

pp. 34 ◽

Cited By ~ 2

Author(s):

Ana Ferreira Farias ◽

Alex Lobato Rodrigues ◽

Rosany Lopes Martins ◽

Érica de Menezes Rabelo ◽

Carlos Ferreira Farias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Cytotoxic Activity ◽

Chemical Constituents ◽

Larval Mortality ◽

Chemical Characterization ◽

Artemia Salina ◽

Antioxidant Potential ◽

Tithonia Diversifolia ◽

Bacterial Strains

The present study aimed to evaluate the chemical composition, antioxidant potential, and the cytotoxic and antimicrobial activity of the essential oil of the plant species Tithonia diversifolia (Hemsl) A. Gray. The essential oil obtained was used to identify the chemical compounds present through the techniques of GC-MS and NMR. The antioxidant potential was calculated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl. For cytotoxic activity, the larval mortality of Artemia salina was evaluated. The main chemical constituents identified are αpinene (9.9%), Limonene (5.40%), (Z)-β-ocimene (4.02%), p-cymen-8-ol (3.0%), Piperitone (11.72%), (E)-nerolidol (3.78%) and Spathulenol (10.8%). In the evaluation of the antimicrobial activity, bacterial strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used. The results showed that the bacterium E. coli were more susceptible to the presence of the essential oil, presenting minimal inhibitory concentration at the concentrations that were exposed. The essential oil presented antioxidant activity of 54.6% at the concentration of 5 mg·mL−1 and provided a CI50 of 4.30. It was observed that the essential oil of this species was highly toxic against A. salina lavas, as its cytotoxic activity showed an LC50 of 3.11. Thus, it is concluded that T. diversifolia oils are effective in inhibiting bacterial growth and reducing oxidative stress.

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Evaluation of the Larvicidal Potential of the Essential Oil Pogostemon cablin (Blanco) Benth in the Control of Aedes aegypti

Pharmaceuticals ◽

10.3390/ph12020053 ◽

2019 ◽

Vol 12 (2) ◽

pp. 53 ◽

Cited By ~ 1

Author(s):

Lizandra Lima Santos ◽

Lethicia Barreto Brandão ◽

Rosany Lopes Martins ◽

Erica de Menezes Rabelo ◽

Alex Lobato Rodrigues ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Chemical Constituents ◽

Chemical Characterization ◽

Artemia Salina ◽

World Health ◽

Pogostemon Cablin

The objective of this work was to collect information on the chemical constituents that demonstrate the larvicidal activity against Aedes aegypti, as well as the antioxidant, microbiological, and cytotoxicity potential of the essential oil of Pogostemon cablin leaves. The chemical characterization was performed by gas chromatography coupled to mass spectrometer (GC-MS). The larvicidal activity was performed according to the protocol of the World Health Organization. The antioxidant activity was evaluated through the sequestering capacity of 2,2-diphenyl-1-picryl-hydrazine (DPPH). As for the microbiological evaluation, the microdilution technique was used, according to the protocol of the Clinical and Laboratory Standards Institute. The cytotoxic activity was evaluated against the larvae of Artemia salina. The species P. cablin presented the following compounds: Patchouli alcohol (33.25%), Seyshellene (6.12%), α-bulnesene (4.11%), Pogostol (6.33%), and Norpatchoulenol (5.72%), which was in synergy with the other substances may significantly potentiate the larvicidal action of the species with the LC50 of 28.43 μg·mL−1. There was no antioxidant activity, however, it presented antimicrobial activity against all bacteria tested with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of 62.5 μg·mL−1. The species demonstrated significant toxic action with LC50 of 24.25 μg·mL−1. Therefore, the P. cablin species showed significant larvicidal potential, antimicrobial activity, the absence of antioxidant action, and high toxicity.

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ANTIMICROBIAL ACTIVITY AND CHEMICAL CHARACTERIZATION OF LAUREL ESSENTIAL OIL

Arquivos de Ciências Veterinárias e Zoologia da UNIPAR ◽

10.25110/arqvet.v24i2cont.2021.8512 ◽

2021 ◽

Vol 24 (2cont) ◽

Author(s):

Juliana Olívia Nicolao ◽

Franciana Aparecida Volpato Bellaver ◽

Volmir Kist ◽

Daniel Radin ◽

Marcelo Mendes De Haro ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Salmonella Enterica Serovar Typhimurium ◽

Chemical Characterization ◽

Serovar Typhimurium ◽

Total Composition ◽

And Staphylococcus Aureus

The factors that arouse interest in the study of essential oils as biocidal agents are numerous, such as the fact that they have antibacterial, antifungal, insecticidal, antioxidant, anti-inflammatory and larvicidal properties. The objective of this work was to evaluate the antimicrobial activity, in vitro, of the laurel (Laurus nobilis L) essential oil on the growth of pathogenic bacteria Salmonella enterica serovar Typhimurium ATCC 14028 and Staphylococcus aureus ATCC 25923, at different exposure times, as well as to perform the chemical characterization. Twenty compounds were identified and quantified, representing 96.57% of the total composition. The class of oxygenated monoterpenes represented the majority class of the essential oil, with 1,8-cineol (33.8%) as the substance found in greater quantity, followed by linalool (17.79%). The third constituent in greater quantity was sabinene (12.23%), belonging to the group of monoterpene hydrocarbons. Terpinyl acetate (9.41%) was also considered to be quantitatively representative. Laurel essential oil showed bacteriostatic activity against S. Typhimurium ATCC 14028 and S. aureus ATCC 25923.

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Chemical profile, antimicrobial activity, toxicity on Artemia salina and anti-acetylcholinesterase enzyme essential oil from Bauhinia ungulata L. (Fabaceae) leaves

Journal of Medicinal Plants Research ◽

10.5897/jmpr2016.6120 ◽

2016 ◽

Vol 10 (29) ◽

pp. 442-449 ◽

Cited By ~ 1

Author(s):

R N A Medeiros Sandra ◽

A de Melo Filho Antonio ◽

N R da Costa Habdel ◽

dos Santos Silva Francisco ◽

C dos Santos Ricardo ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Artemia Salina ◽

Chemical Profile ◽

Acetylcholinesterase Enzyme

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Chemical characterization, antioxidant capacity and antimicrobial activity against food related microorganisms of Citrus limon var. pompia leaf essential oil

LWT ◽

10.1016/j.lwt.2016.02.018 ◽

2016 ◽

Vol 69 ◽

pp. 579-585 ◽

Cited By ~ 25

Author(s):

Francesco Fancello ◽

Giacomo Luigi Petretto ◽

Severino Zara ◽

Maria Lina Sanna ◽

Roberta Addis ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Antioxidant Capacity ◽

Chemical Characterization ◽

Citrus Limon ◽

Leaf Essential Oil

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Monitoring of Antimicrobial Activity of Essential Oils Using Molecular Markers

The Open Biotechnology Journal ◽

10.2174/1874070700903010103 ◽

2009 ◽

Vol 3 (1) ◽

pp. 103-107 ◽

Cited By ~ 3

Author(s):

Hend A. Hamedo

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Agents ◽

Random Amplified Polymorphic Dna ◽

Antimicrobial Effect ◽

E Coli ◽

Specific Effects ◽

And Staphylococcus Aureus ◽

Rosemary Essential Oil

Technological application of essential oils, as natural antimicrobial agents, to reduce the effect of pathogenic microorganisms, requires new methods of detection. The present work evaluated the parameters of antimicrobial activity of the essential oils of rosemary (Rosmarinus officinalis) on two pathogenic strains Escherichia coli and Staphylococcus aureus. The MBC and MIC values were of 2.5, 25 μl ml-1, and values of 1.25 and 5 μl ml-1 for the two strains respectively. In this study, an attempt has been made to evaluate randomly amplified polymorphic DNA (RAPD) analysis for its potential to establish antimicrobial effect of rosemary essential oil. For the preliminary assessment, this study compared the effects occurring at molecular levels in E. coli and Staph. aureus exposed to rosemary essential oil at the MIC concentrations for the two organisms. The qualitative modifications arising in random amplified polymorphic DNA (RAPD) profiles as a measure of DNA effects were compared with control which showed many differences. In conclusion, the measurement of parameters at molecular levels is valuable for investigating the specific effects of agents interacting with DNA.

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Chemical Characterization and Antimicrobial Activity of Essential oil from the Leaves of Bienertia cycloptera

Chemistry of Natural Compounds ◽

10.1007/s10600-016-1824-z ◽

2016 ◽

Vol 52 (5) ◽

pp. 936-938 ◽

Cited By ~ 1

Author(s):

Mohammad Ali Farboodniay Jahromi ◽

Hamed Etemadfard ◽

Zahra Zebarjad

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Chemical Characterization

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Brocchia cinerea essential oil from Brezina (Algerian Sahara): Chemical characterization and antibacterial activity

10.47890/jcsce/2020/acheriti/10082113 ◽

2020 ◽

Vol 1 (1) ◽

Author(s):

Cheriti A ◽

Boukhobza Z ◽

Boulenouar N ◽

Djeradi H ◽

M Kacimi El Hassani ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Chemical Characterization ◽

Klebsiella Pneumonia ◽

Gram Positive Bacteria ◽

Oxygenated Monoterpenes ◽

Algerian Sahara ◽

Total Oil ◽

Diffusion Assay ◽

And Staphylococcus Aureus

The essential oil of wild-growing medicinal specie Brocchia cinerea (Asteraceae) from Brezina (Algerian Sahara) was studied for chemical composition by GC/MS analysis and antibacterial activity. Twenty-nine identified components constituted 94.07% of the oil. The most important major components in the oil were trans-Thujone (36.11 %), Camphor (12.08 %), Santolina triene (11.25 %), 1,8-Cineol (6.98 %) and Cis-Verbenyl acetate (6.33 %), together reaching 72.75% of the total oil which is characterized by the presence of high content of oxygenated monoterpenes. The antibacterial activity of the essential oil was tested using the disc diffusion assay. The results showed that the essential oil of Brocchia cinerea had antibacterial activity against all tested Gram-positive bacteria (Enterococcus faecalis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Klebsiella pneumonia, and Pseudomonas aereginosa). Keywords: Essential Oil; Brocchia Cinerea; GC/MS; Oxygenated Monoterpenes; Antibacterial activity; Sahara;

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