scholarly journals Larvicidal Evaluation against Aedes aegypti and Antioxidant and Cytotoxic Potential of the Essential Oil of Tridax procumbens L. Leaves

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Lethicia B. Brandão ◽  
Lizandra L. Santos ◽  
Rosany L. Martins ◽  
Alex B. L. Rodrigues ◽  
Erica de M. Rabelo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Cytotoxic Activity ◽  
Artemia Salina ◽  
Stage Of Development ◽  
Tridax Procumbens ◽  
Third Stage ◽  
Low Toxicity ◽  
Larvicidal Bioassay

The present study evaluated the antioxidant, cytotoxic, and larvicidal potential of the essential oil of Tridax procumbens leaves, as well as identified the compounds present in the essential oil. The antioxidant activity was evaluated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl radical, the cytotoxic activity was evaluated using Artemia salina, the larvicidal bioassay was performed with larvae in the third stage of development of the Aedes aegypti mosquito, and the identification of the metabolites was performed by gas chromatography coupled to the mass spectrometer (GC-MS). The phytochemical oil analysis showed the presence of 20 compounds, with thymol and γ-terpinene being the main ones. It presented antioxidant activity with an IC50 of 194.51 μg mL−1, demonstrating antioxidant activity in the highest concentrations tested. It presented low cytotoxic activity against A. salina, with an LC50 of 1238.67 μg mL−1, demonstrating atoxicity in the concentrations tested. The essential oil presented good larvicidal activity when compared to the literature, with an LC50 = 79.0 μg mL−1 in 24 hours and LC50 of 69.15 μg mL−1 in 48 hours. In this way, it was possible to identify that the essential oil of the leaves of T. procumbens presented potential for the development of a natural larvicide, as well as antioxidant activity satisfactory to the radical DPPH and low toxicity to A. salina.

scholarly journals Chemical Composition andIn VitroAntioxidant, Cytotoxic, Antimicrobial, and Larvicidal Activities of the Essential Oil ofMentha piperitaL. (Lamiaceae)

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Ryan da Silva Ramos ◽  
Alex Bruno Lobato Rodrigues ◽  
Ana Luzia Ferreira Farias ◽  
Ranggel Carvalho Simões ◽  
Mayara Tânia Pinheiro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Artemia Salina ◽  
Significant Inhibition ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Stage Of Development ◽  
Third Stage ◽  
Larvicidal Activities

The essential oil was obtained by hydrodistillation and the identification and quantification of components were achieved with the use of GC-MS analysis. The antioxidant activity was evaluated by the method of sequestration of DPPH. Essential oils were used for study the cytotoxic front larvae ofArtemia salina. In the evaluation of the antimicrobial activity of essential oils, we employed the disk-diffusion method. The potential larvicide in mosquito larvae of the third stage of development ofAedes aegyptito different concentrations of essential oils was evaluated. The major compounds found in the essential oils ofM. piperitawere linalool (51.8%) and epoxyocimene (19.3%). The percentage of antioxidant activity was79.9±1.6%. The essential oil showed LC50= 414.6 μg/mL front ofA. salineand is considered highly toxic. It shows sensitivity and halos significant inhibition againstE. coli. The essential possessed partial larvicidal efficiency againstA. aegypti.

scholarly journals Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

2019 ◽  
Author(s):  
Renata do Socorro Barbosa Chaves ◽  
Rosany Lopes Martins ◽  
Alex Bruno Lobato Rodrigues ◽  
Érica de Menezes Rabelo ◽  
Ana Luzia Ferreira Farias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Artemia Salina ◽  
High Cytotoxic Activity ◽  
Origanum Majorana ◽  
Main Component

ABSTRACTThis study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

scholarly journals Evaluation of the Larvicidal Potential of the Essential Oil Pogostemon cablin (Blanco) Benth in the Control of Aedes aegypti

2019 ◽  
Vol 12 (2) ◽  
pp. 53 ◽  
Author(s):  
Lizandra Lima Santos ◽  
Lethicia Barreto Brandão ◽  
Rosany Lopes Martins ◽  
Erica de Menezes Rabelo ◽  
Alex Lobato Rodrigues ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Chemical Constituents ◽  
Chemical Characterization ◽  
Artemia Salina ◽  
World Health ◽  
Pogostemon Cablin

The objective of this work was to collect information on the chemical constituents that demonstrate the larvicidal activity against Aedes aegypti, as well as the antioxidant, microbiological, and cytotoxicity potential of the essential oil of Pogostemon cablin leaves. The chemical characterization was performed by gas chromatography coupled to mass spectrometer (GC-MS). The larvicidal activity was performed according to the protocol of the World Health Organization. The antioxidant activity was evaluated through the sequestering capacity of 2,2-diphenyl-1-picryl-hydrazine (DPPH). As for the microbiological evaluation, the microdilution technique was used, according to the protocol of the Clinical and Laboratory Standards Institute. The cytotoxic activity was evaluated against the larvae of Artemia salina. The species P. cablin presented the following compounds: Patchouli alcohol (33.25%), Seyshellene (6.12%), α-bulnesene (4.11%), Pogostol (6.33%), and Norpatchoulenol (5.72%), which was in synergy with the other substances may significantly potentiate the larvicidal action of the species with the LC50 of 28.43 μg·mL−1. There was no antioxidant activity, however, it presented antimicrobial activity against all bacteria tested with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of 62.5 μg·mL−1. The species demonstrated significant toxic action with LC50 of 24.25 μg·mL−1. Therefore, the P. cablin species showed significant larvicidal potential, antimicrobial activity, the absence of antioxidant action, and high toxicity.

scholarly journals Thymol Chemotype Origanum vulgare L. Essential Oil as a Potential Selective Bio-Based Herbicide on Monocot Plant Species

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 595 ◽  
Author(s):  
Daniela Gruľová ◽  
Lucia Caputo ◽  
Hazem S. Elshafie ◽  
Beáta Baranová ◽  
Laura De Martino ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Cytotoxic Activity ◽  
Plant Species ◽  
Pseudomonas Syringae ◽  
Penicillium Expansum ◽  
Chemical Pollution ◽  
Origanum Vulgare ◽  
Promising Source ◽  
Dicot Species

Searching for new bio-based herbicides is crucial for decreasing chemical pollution, protecting the environment, and sustaining biodiversity. Origanum vulgare is considered a promising source of essential oil with herbicidal effect. The mode of action is not known. The present study focused on (1) comparison of phytotoxic activity of Origanum vulgare EO on monocot (Triticum aestivum and Hordeum vulgare) and dicot species (Lepidium sativum and Sinapis alba); (2) and evaluating other antimicrobial biological activities against phytopatogen bacteria (Clavibacter michiganensis, Pseudomonas syringae pv. phaseolicola, Pseudomonas savastanoi, and Xanthomonas campestris); antifungal activity against Monilinia fructicola, Aspergillus niger, Penicillium expansum, and Botrytis cinerea; cytotoxic activity and antioxidant activity. According to the GC/MS analyses, the EO belongs to the thymol chemotype O. vulgare with its high content of thymol (76%). Germination of all four species was not influenced by EO. The phytotoxic effect was statistically significant in the monocot species, while in the dicot species the opposite was observed—a stimulation effect, which was also statistically significant. Strong biological activity of O. vulgare EO was noted on all phytopatogen bacteria and fungi in the highest dose. Cytotoxic activity showed an IC50 = 50.5 μg/mL. Antioxidant activity showed an IC50 = 106.6 μg/mL after 45 min experimental time. Based on the presented results, it is possible to conclude that thymol chemotype O. vulgare essential oil could be potentially used as a herbicide with selective effects on monocot plant species.

scholarly journals Chemical Characterization, Antioxidant, Cytotoxic and Microbiological Activities of the Essential Oil of Leaf of Tithonia Diversifolia (Hemsl) A. Gray (Asteraceae)

2019 ◽  
Vol 12 (1) ◽  
pp. 34 ◽  
Author(s):  
Ana Ferreira Farias ◽  
Alex Lobato Rodrigues ◽  
Rosany Lopes Martins ◽  
Érica de Menezes Rabelo ◽  
Carlos Ferreira Farias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Cytotoxic Activity ◽  
Chemical Constituents ◽  
Larval Mortality ◽  
Chemical Characterization ◽  
Artemia Salina ◽  
Antioxidant Potential ◽  
Tithonia Diversifolia ◽  
Bacterial Strains

The present study aimed to evaluate the chemical composition, antioxidant potential, and the cytotoxic and antimicrobial activity of the essential oil of the plant species Tithonia diversifolia (Hemsl) A. Gray. The essential oil obtained was used to identify the chemical compounds present through the techniques of GC-MS and NMR. The antioxidant potential was calculated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl. For cytotoxic activity, the larval mortality of Artemia salina was evaluated. The main chemical constituents identified are αpinene (9.9%), Limonene (5.40%), (Z)-β-ocimene (4.02%), p-cymen-8-ol (3.0%), Piperitone (11.72%), (E)-nerolidol (3.78%) and Spathulenol (10.8%). In the evaluation of the antimicrobial activity, bacterial strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used. The results showed that the bacterium E. coli were more susceptible to the presence of the essential oil, presenting minimal inhibitory concentration at the concentrations that were exposed. The essential oil presented antioxidant activity of 54.6% at the concentration of 5 mg·mL−1 and provided a CI50 of 4.30. It was observed that the essential oil of this species was highly toxic against A. salina lavas, as its cytotoxic activity showed an LC50 of 3.11. Thus, it is concluded that T. diversifolia oils are effective in inhibiting bacterial growth and reducing oxidative stress.

scholarly journals Leaf Essential Oil of Manekia naranjoana (Piperaceae) from Costa Rica and its Cytotoxic Activity

2011 ◽  
Vol 6 (1) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Carlos Chaverri ◽  
Cecilia Díaz ◽  
José F. Cicció
Keyword(s):  
Costa Rica ◽  
Chemical Composition ◽  
Essential Oil ◽  
Tumor Cell ◽  
Cytotoxic Activity ◽  
Tumor Cell Lines ◽  
Capillary Gc ◽  
Leaf Oil ◽  
Leaf Essential Oil ◽  
Low Toxicity

The chemical composition of the leaf oil of Manekia naranjoana (C. DC.) Callejas (Piperaceae) from Costa Rica was analyzed by capillary GC/FID and GC/MS. Fifty-five compounds were identified. Major compounds from the leaf oil were β-pinene (30.6%), α-pinene (18.8%), limonene (13.7%), and β-caryophyllene (6.1%). The oil presented very low toxicity to tumor and non-tumor cell lines, even though it contains components, such as α- and β-pinene, limonene and others, which have been shown to be cytotoxic. This is the first report of the chemical composition of the essential oil obtained from this species.

scholarly journals PHYTOCHEMICAL, ANTI-INFLAMMATORY, ANTIOXIDANT, CYTOTOXIC AND ANTIBACTERIAL STUDY OF CAPPARIS CARTILAGINEA DECNEFROM YEMEN

2018 ◽  
Vol 10 (6) ◽  
pp. 38
Author(s):  
Bushra Abdulkarim Moharram ◽  
Hassan M. Al-mahbashi ◽  
Riyadh Saif Ali ◽  
Faten Ali Aqlan
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Cytotoxic Activity ◽  
Human Lung ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Paw Edema ◽  
Anti Inflammatory

Objective: To investigate phytochemicals and biological activities of Capparis cartilaginea extracts.Methods: The methanolic extracts of leaves, stem and twigs of C. cartilaginea were screened for their phytochemicals. The essential oil of the leaves was hydrodistilled by a Clevenger apparatus and analyzed by gas chromatography-mass spectrometry (GC-MS). The leaves extract of C. cartilaginea was evaluated for its anti-inflammatory effect, using formalin-induced paw edema. The leaves, stem and twig extracts were assessed for their antioxidant activity, using free radical scavenging assay, cytotoxic activity, using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and antibacterial activity, using the microdilution method.Results: All extracts of C. cartilaginea contained alkaloids, carbohydrates, protein, coumarin, phytosterols, bitter principles, phenols and tannins. The essential oil of the leaves was mainly composed of isopropyl isothiocyanate (69.4%), butane,1-isothiocyanate (26.97%) and isobutyl isothiocyanate (3.26%). The leaves extract at doses of 200 and 400 mg/kg, significantly inhibited paw edema at the 3rd h (49.1%, 54.0%, respectively) and this effect was comparable to that of diclofenac (58.87%). The leaves extract showed the highest antioxidant activity with IC50 value of 91.71 µg/ml. The twigs extract exhibited the highest cytotoxic activity against human lung carcinoma (A549) with IC50 of 57.5 µg/ml. The leaves and stem extracts exhibited antibacterial activity against Staphylococcus aureus with minimum inhibitory concentration (MIC) of 5.0 mg/ml. Conclusion: The leaves extract of C. cartilaginea is a potential source of bioactive compounds that could have a role in anti-inflammation. Twigs extract of the C. cartilaginea possesses a potential cytotoxic effect on human lung cell line.

scholarly journals Triagem fitoquímica e avaliação das atividades antioxidante e citotóxica de extratos etanólicos de Malvaviscus arboreus Cav. (MALVACEAE)

2017 ◽  
Vol 2 (1) ◽  
pp. 9-24
Author(s):  
Jhonatan Da Cruz Ribeiro ◽  
Marco Aurélio Sivero Mayworm
Keyword(s):  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Folk Medicine ◽  
Artemia Salina ◽  
Ethanol Solution ◽  
Cytotoxic Activities ◽  
Total Phenols ◽  
Phytochemical Screening ◽  
Root Extracts ◽  
Sedative Activity

Resumo: Malvaviscus arboreus pertencente à família Malvaceae, é utilizada em jardinagem, medicina popular e na culinária. Estudos anteriores demonstraram atividade moluscicida e alelopática de extrato das raízes; e atividade antitussígena, emoliente e sedativa de extrato das fl ores. Este trabalho visou avaliar as atividades antioxidante e citotóxica, e realizar triagem fi toquímica de extratos etanólicos produzidos a partir de segmentos de caule, folhas e fl ores de M. arboreus. A triagem fi toquímica avaliou a presença de fenóis totais, fl avonoides, quinonas, saponinas, alcaloides e antocianinas. A atividade antioxidante foi avaliada utilizando-se solução etanólica de DPPH (2,2-difenil-1-picrilidrazila) e os valores de absorbância foram interpretados em percentuais de atividade antioxidante (AA%). A atividade citotóxica foi avaliada através do teste de letalidade contra Artemia salina Leach. Demonstrou-seque todos os extratos apresentaram fenóis totais e fl avonoides, porém somente o extrato de fl ores apresentou antocianinas. Quanto à atividade antioxidante, o extrato etanólico de folhas mostrou-se o mais ativo nas concentrações de 100 μg/mL, 50 μg/mL e 10 μg/mL, comparado aos demais extratos. Na avaliação citotóxica, o extrato de fl ores demonstrou maior toxicidade sobre Artemia nas duas concentrações testadas (1mg/mL e 0,5 mg/mL). Conclui-se que os extratos de caule, folhas e fl ores de Malvaviscus arboreus têm potenciais antioxidante e citotóxico nas concentrações testadas.Palavras-chaves: Malvaviscus arboreus; Triagem fi toquímica; Atividade antioxidante; Atividade citotóxica.Abstract: Malvaviscus arboreus (family Malvaceae), is used in gardening, folk medicine and in cooking. Previous studies have shown molluscicidal and allelopathic activity of the root extracts, antitussive, emollient and sedative activity from extracts of fl owers. Th is study evaluated the antioxidant and cytotoxic activities and performing phytochemical screening in ethanolic extracts from segments of stem, leaves and fl owers of M. arboreus. Th e phytochemical screening aimed to evaluate the presence of total phenols, fl avonoids, quinones, saponins, alkaloids and anthocyanins. Th e antioxidant activity was evaluated using ethanol solution of DPPH (2,2-diphenyl-1-picrylhydrazyl) and absorbance values were interpreted as a percentage of antioxidant activity (AA%). Th e cytotoxic activity of the extracts was assessed using the lethality test against Artemia salina Leach. Was shown that all extracts showed total phenols and fl avonoids, but only the fl ower extract showed anthocyanins. As the antioxidant activity, the leaves extract was found to be the most active in the concentrations of 100 μg/mL, 50 μg/mL and 10 μg/mL, when compared with other extracts. In the cytotoxic evaluation , the fl ower extract showed greater toxicity on Artemia in the two concentrations tested (1mg/mL and 0,5 mg/mL). It’s concluded that the extracts of stem, leaves and fl owers of Malvaviscus arboreus have antioxidant and cytotoxic potential, at the concentrations tested.Key-words: Malvaviscus arboreus; Phytochemical screening; Antioxidant activity; Cytotoxic activity.

scholarly journals Physicochemical properties, toxicity and larvicidal activity of the essential oil of Cymbopogon winterianus in front of Aedes aegypti

2020 ◽  
Vol 42 ◽  
pp. e39
Author(s):  
Gabriela Morais da Costa ◽  
Maria Conceição Carneiro dos Santos ◽  
Gustavo Oliveira Everton ◽  
Paulina De Cássia Duarte de Sousa ◽  
Maurício Eduardo Salgado Rangel
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Physicochemical Properties ◽  
Larvicidal Activity ◽  
Artemia Salina ◽  
Toxicity Assay ◽  
Toxicity Effect ◽  
Highly Active ◽  
Cymbopogon Winterianus ◽  
Current Context

Due to the increasing rate of morbidity and mortality caused by vector diseases in the current context, especially by Aedes aegypti, substances of plants have been studied as alternatives to chemical insecticides, among them, the essential oil (EO) of the species Cymbopogon winterianus. Thus, this study evaluated the larvicidal activity of the EO of C. winterianus against the A. aegypti larvae. EO was extracted through the hydro-distillation technique and physicochemical properties were determined. To evaluate larvicidal activity, tests were performed with larvae in the third instar at the final concentrations of 19.54; 26.50; 55.59; 138.98; 208.47 and 277.97 mg L-1 of C. winterianus EO. In addition, Artemia salina Leach bioassay was used to verify toxicity effect. EO obtained presented satisfactory results  in 2.64%. In the larvicidal assay, 100% mortality of larvae was observed after 24 hours at concentrations of 208.47 mg L-1 and 277.97 mg L-1 of the EO, showed effective in the other concentrations and with LC50 of 46.18 mg L-1, considered highly active. In the toxicity assay, the EO presented LC50 at 532.34 mg L-1, showed considered nontoxic. These results reinforce the use of EO front A. aegypt larvae control. 

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