Physicochemical properties, toxicity and larvicidal activity of the essential oil of Cymbopogon winterianus in front of Aedes aegypti

Due to the increasing rate of morbidity and mortality caused by vector diseases in the current context, especially by Aedes aegypti, substances of plants have been studied as alternatives to chemical insecticides, among them, the essential oil (EO) of the species Cymbopogon winterianus. Thus, this study evaluated the larvicidal activity of the EO of C. winterianus against the A. aegypti larvae. EO was extracted through the hydro-distillation technique and physicochemical properties were determined. To evaluate larvicidal activity, tests were performed with larvae in the third instar at the final concentrations of 19.54; 26.50; 55.59; 138.98; 208.47 and 277.97 mg L-1 of C. winterianus EO. In addition, Artemia salina Leach bioassay was used to verify toxicity effect. EO obtained presented satisfactory results in 2.64%. In the larvicidal assay, 100% mortality of larvae was observed after 24 hours at concentrations of 208.47 mg L-1 and 277.97 mg L-1 of the EO, showed effective in the other concentrations and with LC50 of 46.18 mg L-1, considered highly active. In the toxicity assay, the EO presented LC50 at 532.34 mg L-1, showed considered nontoxic. These results reinforce the use of EO front A. aegypt larvae control.

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Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

10.1101/595900 ◽

2019 ◽

Cited By ~ 1

Author(s):

Renata do Socorro Barbosa Chaves ◽

Rosany Lopes Martins ◽

Alex Bruno Lobato Rodrigues ◽

Érica de Menezes Rabelo ◽

Ana Luzia Ferreira Farias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Artemia Salina ◽

High Cytotoxic Activity ◽

Origanum Majorana ◽

Main Component

ABSTRACTThis study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

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Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina

Eclética Química Journal ◽

10.26850/1678-4618eqj.v44.4.2019.p85-95 ◽

2019 ◽

Vol 44 (4) ◽

pp. 85 ◽

Cited By ~ 1

Author(s):

Paulo Roberto Barros Gomes ◽

Marlucy Bezerra Oliveira ◽

Dionney Andrade De Sousa ◽

Jeremias Caetano Da Silva ◽

Romer Pessôa Fernandes ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Biological Activities ◽

Biomphalaria Glabrata ◽

Artemia Salina ◽

World Health ◽

Lethal Concentration ◽

Major Constituent ◽

Citrus Limon

In this present work, we tested the larvicidal activity, molluscicide and toxicity of the oil extracted from Citrus limon peels, respectively against third stage larvae of Aedes aegypti, snail Biomphalaria glabrata, and Artemia salina. For this, we extract the essential oil by hydrodistillation. Then, we identified and quantified the components by gas chromatography coupled to mass spectrometry (GC-MS). We tested the larvicidal and molluscicidal activity, respectively, using the method adopted by the Brazilian Ministry of Health and the World Health Organization. We calculated the lethal concentration (LC50) from the Probit method for the three biological activities with 95 %. The results of the chromatographic analysis showed that the oil has 58.81% of Limonene (major constituent) and 0.11% α-Mulene (minority component). The essential oil presented lethal concentration (LC50) for larvicidal activity, molluscicide and toxicity, respectively at 15.48, 13.05 and 743.35 mg∙L-1. Therefore, the essential oil is active against larvae of A. aegypti and snail B. glabrata and non-toxic against larvae of A. salina.

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Evaluation of the Larvicidal Potential of the Essential Oil Pogostemon cablin (Blanco) Benth in the Control of Aedes aegypti

Pharmaceuticals ◽

10.3390/ph12020053 ◽

2019 ◽

Vol 12 (2) ◽

pp. 53 ◽

Cited By ~ 1

Author(s):

Lizandra Lima Santos ◽

Lethicia Barreto Brandão ◽

Rosany Lopes Martins ◽

Erica de Menezes Rabelo ◽

Alex Lobato Rodrigues ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Chemical Constituents ◽

Chemical Characterization ◽

Artemia Salina ◽

World Health ◽

Pogostemon Cablin

The objective of this work was to collect information on the chemical constituents that demonstrate the larvicidal activity against Aedes aegypti, as well as the antioxidant, microbiological, and cytotoxicity potential of the essential oil of Pogostemon cablin leaves. The chemical characterization was performed by gas chromatography coupled to mass spectrometer (GC-MS). The larvicidal activity was performed according to the protocol of the World Health Organization. The antioxidant activity was evaluated through the sequestering capacity of 2,2-diphenyl-1-picryl-hydrazine (DPPH). As for the microbiological evaluation, the microdilution technique was used, according to the protocol of the Clinical and Laboratory Standards Institute. The cytotoxic activity was evaluated against the larvae of Artemia salina. The species P. cablin presented the following compounds: Patchouli alcohol (33.25%), Seyshellene (6.12%), α-bulnesene (4.11%), Pogostol (6.33%), and Norpatchoulenol (5.72%), which was in synergy with the other substances may significantly potentiate the larvicidal action of the species with the LC50 of 28.43 μg·mL−1. There was no antioxidant activity, however, it presented antimicrobial activity against all bacteria tested with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of 62.5 μg·mL−1. The species demonstrated significant toxic action with LC50 of 24.25 μg·mL−1. Therefore, the P. cablin species showed significant larvicidal potential, antimicrobial activity, the absence of antioxidant action, and high toxicity.

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Repellent and Larvicidal Activity of the Essential Oil From Eucalyptus nitens Against Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

Journal of Medical Entomology ◽

10.1093/jme/tjw222 ◽

2017 ◽

Vol 54 (3) ◽

pp. 670-676 ◽

Cited By ~ 15

Author(s):

Agustín Alvarez Costa ◽

Cecilia V. Naspi ◽

Alejandro Lucia ◽

Héctor M. Masuh

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Aedes Albopictus ◽

Eucalyptus Nitens

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Larvicidal activity against Aedes aegypti of essential oil of Laurus nobilis leaves obtained at different seasons

Journal of Essential Oil Research ◽

10.1080/10412905.2018.1473294 ◽

2018 ◽

Vol 30 (5) ◽

pp. 379-387 ◽

Cited By ~ 2

Author(s):

Carla Maria Mariano Fernandez ◽

Maurício Ferreira da Rosa ◽

Ana Claudia Aparecida Mariano Fernandez ◽

Fabiana Brusco Lorenzetti ◽

Keila Fernanda Raimundo ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Laurus Nobilis ◽

Different Seasons

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Development of an Environmentally Friendly Larvicidal Formulation Based on Essential Oil Compound Blend to Control Aedes aegypti Larvae: Correlations between Physicochemical Properties and Insecticidal Activity

ACS Sustainable Chemistry & Engineering ◽

10.1021/acssuschemeng.0c03778 ◽

2020 ◽

Author(s):

Alejandro Lucia ◽

Clemence Girard ◽

Micaela Fanucce ◽

Carlos Coviella ◽

Ramón G. Rubio ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Physicochemical Properties ◽

Insecticidal Activity ◽

Environmentally Friendly

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LARVICIDAL ACTIVITY OF CROTON RHAMNIFOLIOIDES(PAX HOFFM) ESSENTIAL OIL ENCAPSULATED IN A POLYMERIC NANOSYSTEM AGAINST THE MOSQUITO AEDES AEGYPTI

Periódico Tchê Química ◽

10.52571/ptq.v17.n34.2020.19_p34_pgs_1_9.pdf ◽

2020 ◽

Vol 17 (34) ◽

pp. 1-9

Author(s):

Andréia Gregório da Silva SANTOS ◽

Daniela do Amaral Ferraz NAVARRO ◽

Nereide Stela Santos MAGALHÃES ◽

Maria Alice Varjal MELO-SANTOS ◽

Sofia Suely Ferreira BRANDÃO

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Aqueous Suspension ◽

New Products ◽

Wide Distribution ◽

Average Content ◽

Plant Origin ◽

Insecticidal Action ◽

Time Of Collection

Aedes aegypti is a species of mosquito with wide distribution worldwide, involved in the cycles of transmission of dengue, Zika and chikungunya. The lack of vaccines for most of these arboviruses highlights the importance of vector control as a measure to reduce the incidence of these diseases. Several researches have been carried out in order to find substances of plant origin that are candidates for the control of A. aegypti. In this sense, medicinal plants that also have an insecticidal action have shown promise for the development of new products, environmentally safer than traditional chemical insecticides. The objective of this work was to evaluate the larvicidal activity of the essential oil of the leaves of Croton rhamnifolioides and its formulation in nanocapsules for A. aegypti. The essential oil was extracted by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer (CG/MS). Formulations were produced containing concentrations of pure oil, which ranged from 40 to 80 μg/mL and polycaprolactone nanocapsules with concentrations from 30 to 120 μg/mL. Larvicide tests were performed using varying concentrations of an aqueous suspension, in triplicates, containing 20 larvae / concentration, in addition to an untreated control. As a result, the essential oil had an average content that varied between 1.4% and 0.58%, depending on the time of collection and, as main constituents, eucalyptol (16.57%) and (E) -cariophylene (11, 32%). LC50 values ranged from 26.3 μg/mL to 52.2 μg/mL and CL90 from 37.8 μg/mL to 68.7 μg/mL for pure oil. In encapsulated oil, the LC50 = 63.4 μg/mL and the LC90 = 104.8 μg/mL. The pure oil of Croton rhamnifolioides was considered more active against the larvae of A. aegypti than the encapsulated one, although both have great potential for application as a larvicide.

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Larvicidal Evaluation against Aedes aegypti and Antioxidant and Cytotoxic Potential of the Essential Oil of Tridax procumbens L. Leaves

The Scientific World JOURNAL ◽

10.1155/2021/2172919 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Lethicia B. Brandão ◽

Lizandra L. Santos ◽

Rosany L. Martins ◽

Alex B. L. Rodrigues ◽

Erica de M. Rabelo ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Aedes Aegypti ◽

Cytotoxic Activity ◽

Artemia Salina ◽

Stage Of Development ◽

Tridax Procumbens ◽

Third Stage ◽

Low Toxicity ◽

Larvicidal Bioassay

The present study evaluated the antioxidant, cytotoxic, and larvicidal potential of the essential oil of Tridax procumbens leaves, as well as identified the compounds present in the essential oil. The antioxidant activity was evaluated by the sequestration method of 2,2-diphenyl-1-picrylhydrazyl radical, the cytotoxic activity was evaluated using Artemia salina, the larvicidal bioassay was performed with larvae in the third stage of development of the Aedes aegypti mosquito, and the identification of the metabolites was performed by gas chromatography coupled to the mass spectrometer (GC-MS). The phytochemical oil analysis showed the presence of 20 compounds, with thymol and γ-terpinene being the main ones. It presented antioxidant activity with an IC50 of 194.51 μg mL−1, demonstrating antioxidant activity in the highest concentrations tested. It presented low cytotoxic activity against A. salina, with an LC50 of 1238.67 μg mL−1, demonstrating atoxicity in the concentrations tested. The essential oil presented good larvicidal activity when compared to the literature, with an LC50 = 79.0 μg mL−1 in 24 hours and LC50 of 69.15 μg mL−1 in 48 hours. In this way, it was possible to identify that the essential oil of the leaves of T. procumbens presented potential for the development of a natural larvicide, as well as antioxidant activity satisfactory to the radical DPPH and low toxicity to A. salina.

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