Antifungal Activity of Extracts, Fractions, and Constituents from Coccoloba cowellii Leaves

Coccoloba cowellii Britton (Polygonaceae, order Caryophyllales) is an endemic and critically endangered plant species that only grows in the municipality of Camagüey, a province of Cuba. A preliminary investigation of its total methanolic extract led to the discovery of promising antifungal activity. In this study, a bioassay-guided fractionation allowed the isolation of quercetin and four methoxyflavonoids: 3-O-methylquercetin, myricetin 3,3′,4′-trimethyl ether, 6-methoxymyricetin 3,4′-dimethyl ether, and 6-methoxymyricetin 3,3′,4′-trimethyl ether. The leaf extract, fractions, and compounds were tested against various fungi and showed strong in vitro antifungal activity against Cryptococcus neoformans and various Candida spp. with no cytotoxicity (CC50 > 64.0 µg/mL) on MRC-5 SV2 cells, determined by a resazurin assay. A Candida albicans SC5314 antibiofilm assay indicated that the antifungal activity of C. cowellii extracts and constituents is mainly targeted to planktonic cells. The total methanolic extract showed higher and broader activity compared with the fractions and mixture of compounds.

Download Full-text

Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro

Pharmaceutics ◽

10.3390/pharmaceutics13070998 ◽

2021 ◽

Vol 13 (7) ◽

pp. 998

Author(s):

Virgínia Barreto Lordello ◽

Andréia Bagliotti Meneguin ◽

Sarah Raquel de Annunzio ◽

Maria Pía Taranto ◽

Marlus Chorilli ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Enterococcus Faecium ◽

Potato Starch ◽

Oral Candidiasis ◽

Candida Spp ◽

Sem Images ◽

Control Film ◽

In Vitro Antifungal Activity

Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.

Download Full-text

In-vitro antifungal activity of sertaconazole, econazole, and bifonazole against Candida spp

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/36.4.713 ◽

1995 ◽

Vol 36 (4) ◽

pp. 713-716 ◽

Cited By ~ 12

Author(s):

A. J. Carrillo-Muñioz ◽

J. M. Torres-Rodriguez

Keyword(s):

Antifungal Activity ◽

Candida Spp ◽

In Vitro Antifungal Activity

Download Full-text

Activity of Armillarisin B in vitro against Plant Pathogenic Fungi

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-11-1206 ◽

2009 ◽

Vol 64 (11-12) ◽

pp. 790-792 ◽

Cited By ~ 2

Author(s):

Jin-Wen Shen ◽

Bing-Ji Ma ◽

Wen Li ◽

Hai-You Yu ◽

Ting-Ting Wu ◽

...

Keyword(s):

Antifungal Activity ◽

Methanolic Extract ◽

Pathogenic Fungi ◽

2D Nmr ◽

Spectroscopic Studies ◽

Gibberella Zeae ◽

Plant Pathogenic Fungi ◽

Fruiting Bodies ◽

Bioassay Guided Fractionation

The methanolic extract of the fruiting bodies of the mushroom Armillariella tabescens was found to show antifungal activity against Gibberella zeae. The active compound was isolated from the fruiting bodies of A. tabescens by bioassay-guided fractionation of the extract and identifi ed as armillarisin B. Armillarisin B eventually corresponds to 2-hydroxy-2- phenylpropanediamide and its structure was confi rmed on the basis of spectroscopic studies including 2D NMR experiments.

Download Full-text

Essential oils against Candida spp: in vitro antifungal activity of Origanum vulgare

African Journal of Microbiology Research ◽

10.5897/ajmr12.1149 ◽

2013 ◽

Vol 7 (20) ◽

pp. 2245-2250 ◽

Cited By ~ 6

Author(s):

Brum Cleff Marlete ◽

Madrid Isabel ◽

Raquel Meinerz Ana ◽

Carlos Arauacute jo Meireles Maacute rio ◽

Roberto Braga de Mello Joatilde o ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Origanum Vulgare ◽

Candida Spp ◽

In Vitro Antifungal Activity

Download Full-text

1,3,4-Thiadiazole Derivatives. Part 91. Synthesis and Biological Activity of Metal Complexes of 5-(2-Aminoethyl)-2-Amino-1,3,4-Thiadiazole

Metal-Based Drugs ◽

10.1155/mbd.1996.227 ◽

1996 ◽

Vol 3 (5) ◽

pp. 227-232 ◽

Cited By ~ 20

Author(s):

Mihai Barboiu ◽

Marilena Cimpoesu ◽

Cornelia Guran ◽

Claudiu T. Supuran

Keyword(s):

Biological Activity ◽

Antifungal Activity ◽

Metal Complexes ◽

Elemental Analysis ◽

Electronic Spectroscopy ◽

Candida Spp ◽

Thiadiazole Derivatives ◽

In Vitro Antifungal Activity

Metal complexes of the title ligand (L) containing Co(II), Ni(II) and Cu(II) were prepared and characterized by elemental analysis, IR, electronic spectroscopy and conductimetry. The new derivatives, possessing the following formulae, CuL2(OH)2, NiL2Cl2, and [Co2LCl4]n showed in vitro antifungal activity against Aspergillus and Candida spp.

Download Full-text

Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis

Brazilian Dental Science ◽

10.14295/bds.2018.v21i1.1489 ◽

2018 ◽

Vol 21 (1) ◽

pp. 111 ◽

Cited By ~ 1

Author(s):

Erica Crastechini ◽

Cristiane Yumi Koga-Ito ◽

Suzan De Fátima Machado ◽

Guilherme Rodrigues Teodoro ◽

Graziella Nuernberg Back-Brito ◽

...

Keyword(s):

Antifungal Activity ◽

Olive Oil ◽

In Vitro Tests ◽

Candida Spp ◽

Isolation And Identification ◽

Denture Stomatitis ◽

Viable Cells ◽

In Vitro Antifungal Activity

Objective: The aim of this study was to evaluate the effect of ozonized oil (OZ) on the oral levels of Candida spp. in patients with denture stomatitis. Material and Methods: In vitro tests were performed to validate antifungal activity and to standardize OZ conditions. Antifungal activity was screened against C. albicans and five non-albicans species (C. tropicalis, C. dubliniensis, C. krusei, C. guilliermondii, and C. parapsilosis). Also, the effects on C. albicans planktonic and biofilm were evaluated. After validation, OZ was included in a therapeutic protocol of denture stomatitis in vivo. Thirty patients used OZ and 20 used sodium bicarbonate (SB) for 14 days. After 7 and 14 days, clinical evaluation, isolation and identification of yeasts were performed. Isolates were identified by phenotypic and genotypic tests. Ozonized oil showed in vitro antifungal activity against all species of Candida. Ozonized oil reduced the number of viable cells in C. albicans biofilms. Oral candidal levels were lower in relation to baseline both after after 14 days of treatment with SB and OZ. Results: A total of 493 Candida spp. isolates was obtained and 80% were identified as C. albicans. Remission of denture stomatitis was observed in all patients after 7 days of treatment in both groups. Conclusion: Within the limits of the study we can conclude that ozonized olive oil can be a new alternative for the control of biofilm in patients with denture stomatitis. KeywordsOzone; Candida; Antifungal Agents; Stomatitis; Denture.

Download Full-text

Evaluation of the antifungal activity in vitro of midazolam against fluconazole-resistant Candida spp. isolates

Future Microbiology ◽

10.2217/fmb-2020-0080 ◽

2021 ◽

Vol 16 (2) ◽

pp. 71-81

Author(s):

Maria AV Holanda ◽

Cecília R da Silva ◽

João B de A Neto ◽

Lívia G do AV Sá ◽

Francisca BSA do Nascimento ◽

...

Keyword(s):

Antifungal Activity ◽

Probable Mechanism ◽

Cytotoxicity Test ◽

Broth Microdilution ◽

Planktonic Cells ◽

Candida Spp ◽

Antifungal Potential ◽

Fluconazole Resistant ◽

Scanning Electron

Aim: The purpose of this study was to evaluate the antifungal activity of midazolam, alone and in association with azoles, against isolates of clinical Candida spp. in planktonic and biofilm form. Materials & methods: The antifungal activity was observed using the broth microdilution technique. Flow cytometry tests were performed to investigate the probable mechanism of action and the comet test and cytotoxicity test were applied to evaluate DNA damage. Results: Midazolam (MIDAZ) showed antifungal activity against planktonic cells (125–250 μg/ml) and reduced the viability of Candida spp. biofilms (125 a 2500 μg/ml). The interaction of MIDAZ against Candida spp. biofilms was observed through scanning electron microscopy, causing alteration of their appearance. Therefore, MIDAZ has antifungal potential against Candida spp.

Download Full-text

Rutaceae: conservation at Eka Karya Bali Botanic Garden and its in vitro antifungal activity screening

Ilmu Pertanian (Agricultural Science) ◽

10.22146/ipas.60369 ◽

2021 ◽

Vol 6 (2) ◽

pp. 108

Author(s):

I Putu Agus Hendra Wibawa ◽

Arrohmatus Syafaqoh Li'aini ◽

Putri Sri Andila ◽

Frelyta Ainuz Zahro'

Keyword(s):

Antifungal Activity ◽

Methanolic Extract ◽

Pathogenic Fungi ◽

Inhibitory Effect ◽

Diffusion Method ◽

Botanic Garden ◽

Murraya Paniculata ◽

Activity Screening ◽

In Vitro Antifungal Activity

Several species of Rutaceae have been widely used and commercialized in all regions in Indonesia. Some species of Rutaceae are consumed as fresh fruit and traditional medicine for various kinds of diseases, as well as to add aroma to various Indonesian culinary. Since 1959, Eka Karya Bali Botanic Garden (Eka Karya BBG) has successfully collected dozens of Rutaceae species with unknown potential. In addition to reporting the conservation of Rutaceae in Eka Karya BBG, this study aimed to screen the antifungal activity of Rutaceae methanolic extract toward Aspergillus niger, Cladosporium sp., and Fusarium solani. Leaves of 13 species of Rutaceae (Boenninghausenia sp., Citrus aurantifolia, C. maxima, C. medica, Clausena sp., Melicope sp., Micromelum sp., Murraya paniculata, Toddalia sp., Zanthoxylum sp., Z. alatum, Z. limonella, and Z. ovalifolium) were collected, cleaned, air-dried, soaked in methanol for three days, then evaporated using a rotary evaporator to obtain the plant crude extract. The in vitro inhibitory assay was conducted by the diffusion method. As a result, only C. medica, Clausena sp., and Z. limonella exhibited antifungal activity against those tested fungi. Their antifungal activity increased on day 2 post-treatment but slowly decreased on day 3. Thus, the result of this experiment can be used as preliminary data to researchRutaceae plant extracts as an alternative method to control pathogenic fungi. However, further research is needed to maintain and increase its inhibitory effect.

Download Full-text