Anti-Cancer Potential of Afzelin towards AGS Gastric Cancer Cells

Afzelin demonstrates anti-inflammatory and anti-cancer properties. Our purpose was to assess its influence on apoptosis, Bax, caspases, MUC1, cancer-related carbohydrate antigens, enzymes participating in their formation, and galectin-3 in AGS gastric cancer cells. A total of 60 and 120 μM afzelin was used in all experiments. Flow cytometry was applied to determine apoptotic response. Western blotting and RT PCR were used to detect the expression of mentioned factors. Flavonoid at higher concentration revealed slight apoptotic respond. Bax, caspase-3, -8, -9 increased upon afzelin action. Stimulatory effect of the flavonoid on MUC1 cytoplasmic tail and extracellular domain in cell lysates and on MUC1 gene was revealed. MUC1 release into the culture medium was inhibited by the flavonoid. The 60 μM afzelin dose stimulated GalNAcTL5 protein expression and inhibited C1GalT1. ST6GalNAcT mRNA was inhibited by both flavonoid doses. ST3GalT was inhibited by 120 μM afzelin on protein and mRNA level. Lewisa/b protein was reduced by both afzelin concentrations. FUT3 and FUT4 mRNA was inhibited by 120 μM dose of afzelin. Galectin-3 protein increased in cell lysates and decreased in culture supernatant by 60 and 120 μM flavonoid. Galectin-3 gene expression was stimulated by two used concentrations of afzelin in comparison to control. We conclude that afzelin can be considered as the potential anti-cancer agent, supporting conventional cancer treatment.

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Water soluble polysaccharides from Spirulina platensis exhibited cyto/DNA protection and suppress the growth of gastric cancer cells via modulation of galectin-3

10.1101/2021.12.16.473001 ◽

2021 ◽

Author(s):

Vinayak Uppin ◽

Shylaja M Dharmesh ◽

Sarada R

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

In Silico ◽

Average Molecular Weight ◽

Haemagglutination Inhibition ◽

Water Soluble ◽

Gastric Cancer Cells ◽

Galectin 3 ◽

Nih 3T3

Polysaccharides from natural sources play a significant role in the management of different cancer types including gastric cancer. In this study, we reported the effect of spirulina polysaccharide (Sp) on galectin-3 modulatory activity on gastric cancer cells. The polysaccharide was isolated from the spirulina biomass, characterized, and the in silico, in vitro studies are carried out to assess the bioactivities. The isolated Sp possessed average molecular weight of 1457 kDa, and galactose (42%) as major sugar along with Rhamnose, Arabinose, Xylose, and Mannose. Further, characterization of Sp by FT-IR and NMR spectrum indicated the presence of (β1-4D) galactose sugar with galactoarabinorhamnoglycan backbone. Among the monosaccharides, galactose showed highest binding affinity with galectin-3 protein as evidenced by the in silico interaction study. The obtained Sp, inhibited the proliferation of AGS gastric cancer cells by 48 % without affecting normal NIH/3T3 cells as opposed to doxorubicin, a known anticancer drug. Also, Sp exhibited galectin-3 mediated haemagglutination inhibition with MIC of 9.37 μg/mL compared to galactose 6.25 μg/mL, sugar specific to galectin-3. The Sp treatment significantly (p<0.05) lowered the expression of galectin-3 by 32 % compared to untreated control cells. In addition, Sp exhibited the potent cytoprotection in RBCs, Buccal cells, and DNA exposed to oxidants. Thus, the findings suggest that the polysaccharide from spirulina offer a promising therapeutic strategy in the management of gastric cancer in addition to its currently known nutritional and pharmaceutical applications.

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Ocimum Gratissimum Extract Induces Apoptosis in Gastric Cancer Cells via Modulation of Reactive Oxygen Species and Mitogen-Activated Protein Kinase

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.19:514-519 ◽

2020 ◽

Vol 19 (4) ◽

pp. 514-519

Author(s):

Ming-Jen Sheu ◽

Jen-Ning Tsai ◽

Wai-Lun Tam ◽

Jer-Yuh Liu ◽

Li-Sung Hsu

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Caspase 3 ◽

Mitogen Activated Protein Kinase ◽

High Rate ◽

Anticancer Activities ◽

Gastric Cancer Cells ◽

Cancer Proliferation ◽

Ocimum Gratissimum ◽

Cancer Agent

Given the increasing incidence of gastric cancer and its high rate of metastasis, drug resistance and the mortality rate remain high. Ocimum gratissimum, a botanical species of Ocimum known to exhibit general anti-inflammatory, antioxidant, and anticancer activities has not yet been evaluated for gastric cancer proliferation. In this study, we have demonstrated that O. gratissimum extract significantly reduces the viability of gastric cancer cells by triggering apoptosis, elevating levels of ROS, and enhanced cleavage of poly-ADP-ribose polymerase and caspase-3. Western blot analysis indicated that O. gratissimum extract enhanced the cleavage of PARP and caspase-3. Moreover, O. gratissimum extract inhibited extracellular signal-regulated kinase 1/2 and increased activities of p38, a stress stimulated kinase. In conclusion, our findings show that O. gratissimum extract may be a potential antigastric cancer agent.

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Inhibition of endogenous hydrogen sulfide biosynthesis enhances the anti-cancer effect of 3,3′-diindolylmethane in human gastric cancer cells

Life Sciences ◽

10.1016/j.lfs.2020.118348 ◽

2020 ◽

Vol 261 ◽

pp. 118348 ◽

Cited By ~ 1

Author(s):

Fen Ye ◽

Xue Li ◽

Kang Sun ◽

Wenrong Xu ◽

Haifeng Shi ◽

...

Keyword(s):

Gastric Cancer ◽

Hydrogen Sulfide ◽

Cancer Cells ◽

Human Gastric Cancer ◽

Gastric Cancer Cells ◽

Anti Cancer

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Anti-cancer effects of newly developed chemotherapeutic agent, glycoconjugated palladium (II) complex, against cisplatin-resistant gastric cancer cells

BMC Cancer ◽

10.1186/1471-2407-13-237 ◽

2013 ◽

Vol 13 (1) ◽

Cited By ~ 31

Author(s):

Mamoru Tanaka ◽

Hiromi Kataoka ◽

Shigenobu Yano ◽

Hiromi Ohi ◽

Keisuke Kawamoto ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Chemotherapeutic Agent ◽

Gastric Cancer Cells ◽

Anti Cancer

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Apigenin Induces Autophagy and Cell Death by Targeting EZH2 under Hypoxia Conditions in Gastric Cancer Cells

International Journal of Molecular Sciences ◽

10.3390/ijms222413455 ◽

2021 ◽

Vol 22 (24) ◽

pp. 13455

Author(s):

Tae Woo Kim ◽

Hee Gu Lee

Keyword(s):

Gastric Cancer ◽

Cell Death ◽

Er Stress ◽

Fruits And Vegetables ◽

Molecular Mechanisms ◽

Autophagic Cell Death ◽

Gastric Cancer Cells ◽

Er Stress Response ◽

Anti Cancer ◽

Cancer Agent

Hypoxia is a major obstacle to gastric cancer (GC) therapy and leads to chemoresistance as GC cells are frequently exposed to the hypoxia environment. Apigenin, a flavonoid found in traditional medicine, fruits, and vegetables and an HDAC inhibitor, is a powerful anti-cancer agent against various cancer cell lines. However, detailed mechanisms involved in the treatment of GC using APG are not fully understood. In this study, we investigated the biological activity of and molecular mechanisms involved in APG-mediated treatment of GC under hypoxia. APG promoted autophagic cell death by increasing ATG5, LC3-II, and phosphorylation of AMPK and ULK1 and down-regulating p-mTOR and p62 in GC. Furthermore, our results show that APG induces autophagic cell death via the activation of the PERK signaling, indicating an endoplasmic reticulum (ER) stress response. The inhibition of ER stress suppressed APG-induced autophagy and conferred prolonged cell survival, indicating autophagic cell death. We further show that APG induces ER stress- and autophagy-related cell death through the inhibition of HIF-1α and Ezh2 under normoxia and hypoxia. Taken together, our findings indicate that APG activates autophagic cell death by inhibiting HIF-1α and Ezh2 under hypoxia conditions in GC cells.

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Chrysophanol Inhibits the Progression of Gastric Cancer by Activating NLRP3

10.21203/rs.3.rs-1073406/v1 ◽

2021 ◽

Author(s):

Hou Binfen ◽

Li Zhao ◽

Min Deng

Keyword(s):

Gastric Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Migration And Invasion ◽

Gastric Cancer Cells ◽

Antitumor Effects ◽

Ags Cells ◽

Anti Cancer ◽

Colony Forming Assay

Abstract AimGastric cancer is one of the most common malignant tumors.Chrysophanol has been reported to have antitumor effects on a variety of cancers, but the role of chrysophanol in gastric cancer remains unclear. The aim of this study was to investigate the effects of chrysophanol on proliferation, pyroptosis, migration and invasion of gastric cancer cells.MethodsMKN 28 and AGS cells were treatde with different concentrations of chrysophanol, then cell proliferation, migration,invasion and pyroptosis were decteed by CCK-8, Colony-forming assay, Wound Healing assay, Transwell and flow cytometry, respectively.Subsequently, NLRP3 siRNA was transfected into MKN 28 cells, cell proliferation pyroptosis, migration and invasion were reassessed in these transfected cells. The expression of caspase-1 and IL-1β in the downstream of NLRP3 was detected by qRT PCR and Western blot.ResultsChrysophanol significantly inhibited the proliferation of GC cells, promoted pyroptosis, inhibited cell migration and invasion, and up-regulated the expression level of NLRP3 inflammasome in GC cells. Silencing NLRP3 inhibited the effects of chrysophanol on proliferation, pyroptosis, migration and invasion of MKN 28 cells. Chrysophanol plays an anti-cancer role through high expression of NLRP3.CoclusionsChrysophanol can inhibit the proliferation, migration and invasion of gastric cancer cells by regulating NLRP3, promote the death of gastric cancer cells, and play an anti-tumor role,which is a clinical strategy with great potential for the treatment of gastric cancer.

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