Anti-cancer effects of newly developed chemotherapeutic agent, glycoconjugated palladium (II) complex, against cisplatin-resistant gastric cancer cells

Afzelin demonstrates anti-inflammatory and anti-cancer properties. Our purpose was to assess its influence on apoptosis, Bax, caspases, MUC1, cancer-related carbohydrate antigens, enzymes participating in their formation, and galectin-3 in AGS gastric cancer cells. A total of 60 and 120 μM afzelin was used in all experiments. Flow cytometry was applied to determine apoptotic response. Western blotting and RT PCR were used to detect the expression of mentioned factors. Flavonoid at higher concentration revealed slight apoptotic respond. Bax, caspase-3, -8, -9 increased upon afzelin action. Stimulatory effect of the flavonoid on MUC1 cytoplasmic tail and extracellular domain in cell lysates and on MUC1 gene was revealed. MUC1 release into the culture medium was inhibited by the flavonoid. The 60 μM afzelin dose stimulated GalNAcTL5 protein expression and inhibited C1GalT1. ST6GalNAcT mRNA was inhibited by both flavonoid doses. ST3GalT was inhibited by 120 μM afzelin on protein and mRNA level. Lewisa/b protein was reduced by both afzelin concentrations. FUT3 and FUT4 mRNA was inhibited by 120 μM dose of afzelin. Galectin-3 protein increased in cell lysates and decreased in culture supernatant by 60 and 120 μM flavonoid. Galectin-3 gene expression was stimulated by two used concentrations of afzelin in comparison to control. We conclude that afzelin can be considered as the potential anti-cancer agent, supporting conventional cancer treatment.

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Chrysophanol Inhibits the Progression of Gastric Cancer by Activating NLRP3

10.21203/rs.3.rs-1073406/v1 ◽

2021 ◽

Author(s):

Hou Binfen ◽

Li Zhao ◽

Min Deng

Keyword(s):

Gastric Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Migration And Invasion ◽

Gastric Cancer Cells ◽

Antitumor Effects ◽

Ags Cells ◽

Anti Cancer ◽

Colony Forming Assay

Abstract AimGastric cancer is one of the most common malignant tumors.Chrysophanol has been reported to have antitumor effects on a variety of cancers, but the role of chrysophanol in gastric cancer remains unclear. The aim of this study was to investigate the effects of chrysophanol on proliferation, pyroptosis, migration and invasion of gastric cancer cells.MethodsMKN 28 and AGS cells were treatde with different concentrations of chrysophanol, then cell proliferation, migration,invasion and pyroptosis were decteed by CCK-8, Colony-forming assay, Wound Healing assay, Transwell and flow cytometry, respectively.Subsequently, NLRP3 siRNA was transfected into MKN 28 cells, cell proliferation pyroptosis, migration and invasion were reassessed in these transfected cells. The expression of caspase-1 and IL-1β in the downstream of NLRP3 was detected by qRT PCR and Western blot.ResultsChrysophanol significantly inhibited the proliferation of GC cells, promoted pyroptosis, inhibited cell migration and invasion, and up-regulated the expression level of NLRP3 inflammasome in GC cells. Silencing NLRP3 inhibited the effects of chrysophanol on proliferation, pyroptosis, migration and invasion of MKN 28 cells. Chrysophanol plays an anti-cancer role through high expression of NLRP3.CoclusionsChrysophanol can inhibit the proliferation, migration and invasion of gastric cancer cells by regulating NLRP3, promote the death of gastric cancer cells, and play an anti-tumor role,which is a clinical strategy with great potential for the treatment of gastric cancer.

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The Anti-Cancer Effect of COX-2 Inhibitors on Gastric Cancer Cells

Digestive Diseases and Sciences ◽

10.1007/s10620-007-9787-3 ◽

2007 ◽

Vol 52 (7) ◽

pp. 1713-1721 ◽

Cited By ~ 30

Author(s):

Soo-Jeong Cho ◽

Nayoung Kim ◽

Joo Sung Kim ◽

Hyun Chae Jung ◽

In Sung Song

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Gastric Cancer Cells ◽

Anti Cancer ◽

Cox 2 ◽

Cox 2 Inhibitors

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Anti-cancer effect of combined action of anti-MUC1 and rosmarinic acid in AGS gastric cancer cells

European Journal of Pharmacology ◽

10.1016/j.ejphar.2021.174119 ◽

2021 ◽

Vol 902 ◽

pp. 174119

Author(s):

Iwona Radziejewska ◽

Katarzyna Supruniuk ◽

Anna Bielawska

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Rosmarinic Acid ◽

Gastric Cancer Cells ◽

Anti Cancer ◽

Combined Action

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Bax overexpression enhances cytochrome c release from mitochondria and sensitizes KATOIII gastric cancer cells to chemotherapeutic agent-induced apoptosis.

International Journal of Oncology ◽

10.3892/ijo.16.4.745 ◽

2000 ◽

Cited By ~ 6

Author(s):

H Sawa ◽

T Kobayashi ◽

K Mukai ◽

W Zhang ◽

H Shiku

Keyword(s):

Gastric Cancer ◽

Cytochrome C ◽

Cancer Cells ◽

Chemotherapeutic Agent ◽

Cytochrome C Release ◽

Gastric Cancer Cells ◽

Induced Apoptosis

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Novel anti-cancer role of naphthazarin in human gastric cancer cells

International Journal of Oncology ◽

10.3892/ijo.2011.1195 ◽

2011 ◽

Author(s):

Tae Son

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Human Gastric Cancer ◽

Gastric Cancer Cells ◽

Anti Cancer

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The Herb Medicine Formula “Chong Lou Fu Fang” Increases the Cytotoxicity of Chemotherapeutic Agents and Down-Regulates the Expression of Chemotherapeutic Agent Resistance-Related Genes in Human Gastric Cancer CellsIn Vitro

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nep175 ◽

2011 ◽

Vol 2011 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Yongping Liu ◽

Yang Ling ◽

Wenjing Hu ◽

Li Xie ◽

Lixia Yu ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Cell Lines ◽

Chemotherapeutic Agent ◽

Chemotherapeutic Agents ◽

Human Gastric Cancer ◽

Gastric Cancer Cells ◽

Herb Medicine

The herb medicine formula “Chong Lou Fu Fang” (CLFF) has efficacy in inhibiting the proliferation of human gastric cancerin vitroandin vivo. To explore the potentially useful combination of CLFF with chemotherapeutic agents commonly used in gastric cancer therapy, we assess the interaction between CLFF and these chemotherapeutic agents in both SGC-7901 cell lines and BGC-823 cell lines using a median effect analysis and apoptosis analysis, and we also investigate the influence of CLFF on chemotherapeutic agent-associated gene expression. The synergistic analysis indicated that CLFF had a synergistic effect on the cytotoxicity of 5-fluorouracil (5-FU) in a relative broad dose inhibition range (20–95% fraction affected in SGC-7901cell lines and 5–65% fraction affected in BGC-823 cell lines), while the synergistic interaction between CLFF and oxaliplatin or docetaxel only existed in a low dose inhibition range (≤50% fraction affected in both cell lines). Combination of CLFF and chemotherapeutic agents could also induce apoptosis in a synergistic manner. After 24 h, CLFF alone or CLFF combination with chemotherapeutic agents could significantly suppress the levels of expression of chemotherapeutic agent resistance related genes in gastric cancer cells. Our findings indicate that there are useful synergistic interactions between CLFF and chemotherapeutic agents in gastric cancer cells, and the possible mechanisms might be partially due to the down-regulation of chemotherapeutic agent resistance related genes and the synergistic apoptotic effect.

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