scholarly journals Influence of Cold-TRP Receptors on Cold-Influenced Behaviour

2021 ◽  
Vol 15 (1) ◽  
pp. 42
Author(s):  
Dibesh Thapa ◽  
Brentton Barrett ◽  
Fulye Argunhan ◽  
Susan D. Brain
Keyword(s):  
Body Temperature ◽  
Cold Exposure ◽  
Transient Receptor Potential ◽  
Trp Channels ◽  
Core Body Temperature ◽  
Physical Restraint ◽  
Receptor Potential ◽  
Whole Body ◽  
Direct Role ◽  
Core Body

The transient receptor potential (TRP) channels, TRPA1 and TRPM8, are thermo-receptors that detect cold and cool temperatures and play pivotal roles in mediating the cold-induced vascular response. In this study, we investigated the role of TRPA1 and TRPM8 in the thermoregulatory behavioural responses to environmental cold exposure by measuring core body temperature and locomotor activity using a telemetry device that was surgically implanted in mice. The core body temperature of mice that were cooled at 4 °C over 3 h was increased and this was accompanied by an increase in UCP-1 and TRPM8 level as detected by Western blot. We then established an effective route, by which the TRP antagonists could be administered orally with palatable food. This avoids the physical restraint of mice, which is crucial as that could influence the behavioural results. Using selective pharmacological antagonists A967079 and AMTB for TRPA1 and TRPM8 receptors, respectively, we show that TRPM8, but not TRPA1, plays a direct role in thermoregulation response to whole body cold exposure in the mouse. Additionally, we provide evidence of increased TRPM8 levels after cold exposure which could be a protective response to increase core body temperature to counter cold.

Transient receptor potential ion channels as participants in thermosensation and thermoregulation

2007 ◽  
Vol 292 (1) ◽  
pp. R64-R76 ◽  
Author(s):  
Michael J. Caterina
Keyword(s):  
Ion Channels ◽  
Molecular Mechanisms ◽  
Transient Receptor Potential ◽  
Trp Channels ◽  
Core Body Temperature ◽  
Expression Patterns ◽  
Receptor Potential ◽  
Genetic Ablation ◽  
Transient Receptor ◽  
Living Organisms

Living organisms must evaluate changes in environmental and internal temperatures to mount appropriate physiological and behavioral responses conducive to survival. Classical physiology has provided a wealth of information regarding the specialization of thermosensory functions among subclasses of peripheral sensory neurons and intrinsically thermosensitive neurons within the hypothalamus. However, until recently, the molecular mechanisms by which these cells carry out thermometry have remained poorly understood. The demonstration that certain ion channels of the transient receptor potential (TRP) family can be activated by increases or decreases in ambient temperature, along with the recognition of their heterogeneous expression patterns and heterogeneous temperature sensitivities, has led investigators to evaluate these proteins as candidate endogenous thermosensors. Much of this work has involved one specific channel, TRP vanilloid 1 (TRPV1), which is both a receptor for capsaicin and related pungent vanilloid compounds and a “heat receptor,” capable of directly depolarizing neurons in response to temperatures >42°C. Evidence for a contribution of TRPV1 to peripheral thermosensation has come from pharmacological, physiological, and genetic approaches. In contrast, although capsaicin-sensitive mechanisms clearly influence core body temperature regulation, the specific contribution of TRPV1 to this process remains a matter of debate. Besides TRPV1, at least six additional thermally sensitive TRP channels have been identified in mammals, and many of these also appear to participate in thermosensation. Moreover, the identification of invertebrate TRP channels, whose genetic ablation alters thermally driven behaviors, makes it clear that thermosensation represents an evolutionarily conserved role of this ion channel family.

Infrared thermal imaging as a method to study thermogenesis in the neonatal lamb

2014 ◽  
Vol 54 (9) ◽  
pp. 1497 ◽  
Author(s):  
S. A. McCoard ◽  
H. V. Henderson ◽  
F. W. Knol ◽  
S. K. Dowling ◽  
J. R. Webster
Keyword(s):  
Body Temperature ◽  
Heat Loss ◽  
Cold Exposure ◽  
Thermal Imaging ◽  
Core Body Temperature ◽  
Recovery Period ◽  
Exposure Period ◽  
Skin Surface ◽  
Infrared Thermal Imaging ◽  
Core Body

The combination of heat generation and reducing heat loss from the skin surface is important for maintaining core body temperature in a neonate. Thermogenesis studies traditionally focus on measurement of core body temperature but not the contribution of radiated heat loss at the skin surface. This study aimed to evaluate the utility of using thermal imaging to measure radiated heat loss in newborn lambs. Continuous thermal images of newborn lambs were captured for 30 min each during the baseline (11−18°C), cold-exposure (0°C) and recovery (11−18°C) periods by using an infrared camera. Core body temperature measured by rectal thermometer was also recorded at the end of each period. In all, 7 of the 10 lambs evaluated had reduced rectal temperatures (0.4−1°C) between the baseline and recovery periods, while three maintained body temperature despite cold exposure. During the baseline period, infrared heat loss was relatively stable, followed by a rapid decrease of 5°C within 5 min of cold exposure. Heat loss continued to decrease linearly in the cold-exposure period by a further 10°C, but increased rapidly to baseline levels during the recovery period. A temperature change of between 20°C and 35°C was observed during the study, which was likely to be due to changes in vasoconstriction in the skin to conserve heat. The present study has highlighted the sensitivity of infrared thermal imaging to estimate heat loss from the skin in the newborn lamb and shown that rapid changes in heat loss occur in response to cold exposure.

Central Neural Circuits Orchestrating Thermogenesis, Sleep-Wakefulness States and General Anesthesia States

2021 ◽  
Vol 19 ◽  
Author(s):  
Jiayi Wu ◽  
Daiqiang Liu ◽  
Jiayan Li ◽  
Jia Sun ◽  
Yujie Huang ◽  
...  
Keyword(s):  
General Anesthesia ◽  
Transient Receptor Potential ◽  
Neural Circuits ◽  
Trp Channels ◽  
Core Body Temperature ◽  
Receptor Potential ◽  
Neural Pathways ◽  
General Anesthetics ◽  
Environmental Light ◽  
Transient Receptor

: Great progress has been made in specifically identifying the central neural circuits (CNCs) of the core body temperature (Tcore), sleep-wakefulness states (SWs), and general anesthesia states (GAs), mainly utilizing optogenetic or chemogenetic manipulations. We summarize the neuronal populations and neural pathways of these three CNCs, which gives evidence for the orchestration within these three CNCs, and the integrative regulation of these three CNCs by different environmental light signals. We also outline some transient receptor potential (TRP) channels that function in the CNCs-Tcore and are modulated by some general anesthetics, which makes TRP channels possible targets for addressing the general-anesthetics-induced-hypothermia (GAIH). We suggest this review will provide new orientations for further consummating these CNCs and elucidating the central mechanisms of GAIH.

scholarly journals Thermal conditions experienced during differentiation affect metabolic and contractile phenotypes of mouse myotubes

2016 ◽  
Vol 311 (3) ◽  
pp. R457-R465 ◽  
Author(s):  
Alex G. Little ◽  
Frank Seebacher
Keyword(s):  
Body Temperature ◽  
Cold Exposure ◽  
Metabolic Flux ◽  
Core Body Temperature ◽  
Thermal Conditions ◽  
Glycolytic Flux ◽  
Peripheral Tissues ◽  
Atp Production ◽  
Metabolic Role ◽  
Core Body

Central pathways regulate metabolic responses to cold in endotherms to maintain relatively stable internal core body temperatures. However, peripheral muscles routinely experience temperatures lower than core body temperature, so that it would be advantageous for peripheral tissues to respond to temperature changes independently from core body temperature regulation. Early developmental conditions can influence offspring phenotypes, and here we tested whether developing muscle can compensate locally for the effects of cold exposure independently from central regulation. Muscle myotubes originate from undifferentiated myoblasts that are laid down during embryogenesis. We show that in a murine myoblast cell line (C2C12), cold exposure (32°C) increased myoblast metabolic flux compared with 37°C control conditions. Importantly, myotubes that differentiated at 32°C compensated for the thermodynamic effects of low temperature by increasing metabolic rates, ATP production, and glycolytic flux. Myotube responses were also modulated by the temperatures experienced by “parent” myoblasts. Myotubes that differentiated under cold exposure increased activity of the AMP-stimulated protein kinase (AMPK), which may mediate metabolic changes in response cold exposure. Moreover, cold exposure shifted myosin heavy chains from slow to fast, presumably to overcome slower contractile speeds resulting from low temperatures. Adjusting thermal sensitivities locally in peripheral tissues complements central thermoregulation and permits animals to maintain function in cold environments. Muscle also plays a major metabolic role in adults, so that developmental responses to cold are likely to influence energy expenditure later in life.

Whole-body cryostimulation increases parasympathetic outflow and decreases core body temperature

2014 ◽  
Vol 45 ◽  
pp. 75-80 ◽  
Author(s):  
Pawel Zalewski ◽  
Anna Bitner ◽  
Joanna Słomko ◽  
Justyna Szrajda ◽  
Jacek J. Klawe ◽  
...  
Keyword(s):  
Body Temperature ◽  
Core Body Temperature ◽  
Whole Body ◽  
Core Body

Abstract 3516: Evaluation of whole body hyperthermia system: changes of core body temperature and their effects on immune system

2012 ◽  
Author(s):  
Yasunobu Kobayashi ◽  
Yusuke Ito ◽  
Valentina V. Ostapenko ◽  
Norimasa Matsushita ◽  
Kenichiro Imai ◽  
...  
Keyword(s):  
Immune System ◽  
Body Temperature ◽  
Core Body Temperature ◽  
Whole Body ◽  
Whole Body Hyperthermia ◽  
System Changes ◽  
Core Body

scholarly journals Liver Derived FGF21 Maintains Core Body Temperature During Acute Cold Exposure

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Magdalene Ameka ◽  
Kathleen R. Markan ◽  
Donald A. Morgan ◽  
Lucas D. BonDurant ◽  
Sharon O. Idiga ◽  
...  
Keyword(s):  
Body Temperature ◽  
Cold Exposure ◽  
Core Body Temperature ◽  
Acute Cold Exposure ◽  
Core Body

scholarly journals Changes in TRPV1-Mediated Physiological Function in Rats Systemically Treated With Capsaicin on the Neonate

2020 ◽  
Vol 21 (9) ◽  
pp. 3143 ◽  
Author(s):  
Keun-Yeong Jeong
Keyword(s):  
Body Temperature ◽  
Pain Perception ◽  
Transient Receptor Potential ◽  
Core Body Temperature ◽  
Receptor Potential ◽  
The Body ◽  
Transient Receptor Potential Vanilloid ◽  
Temperature Rhythm ◽  
Transient Receptor ◽  
Body Temperature Increase

Capsaicin is the active component of chili peppers and is a hydrophobic, colorless, odorless, and crystalline to waxy compound. The transient receptor potential vanilloid 1 (TRPV1) is the capsaicin receptor channels that are involved in a variety of functions like transduction and transmission of the physiological stimulus. Subcutaneous injection of capsaicin to a newborn rat leads to involuntary lifelong TRPV1 desensitization. Various physiological changes including sensory and homeostatic actions in the body associated with neonatal capsaicin treatment are induced by direct TRPV1 channel targeting. Interesting changes include unique phenomena such as the reduction in pain perception, abnormal body temperature, increase in infection, infectious or neuropathological itching, and irregular circadian core body temperature rhythm. These symptoms are associated with relatively higher fever or loss of sensory c-fiber related to TRPV1 desensitization. The aforementioned outcomes not only provide a warning about the risk of capsaicin exposure in newborns but also indicate the possible occurrence of relatively rare diseases that are difficult to diagnose. Therefore, Therefore, the present review aims to summarize the unique phenomena caused by systemic capsaicin administration in neonatal rats.

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