scholarly journals Assessment of the Substance Antioxidative Profile by Hyaluronan, Cu(II) and Ascorbate

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1815
Author(s):  
Katarína Valachová ◽  
Ladislav Šoltés
Keyword(s):  
Antioxidant Activity ◽  
Experimental Approach ◽  
Molar Mass ◽  
Oxidative Degradation ◽  
Inhibitory Effect ◽  
Water Soluble ◽  
High Molar Mass ◽  
Initiation And Propagation ◽  
Chain Breaking ◽  
Exogenous Substances

In the minireview presented here, the authors discuss the evaluation of inhibitory effect of substances in the phases of initiation and propagation of high-molar-mass hyaluronan oxidative degradation. The experimental approach should be considered as original since on using a simple experimental assay it is possible to prove both the so-called “preventive” and “chain-breaking” antioxidant activity of investigated water-soluble endo- or exogenous substances.

Aurothiomalate as Preventive and Chain-Breaking Antioxidant in Radical Degradation of High-Molar-Mass Hyaluronan

2011 ◽  
Vol 8 (7) ◽  
pp. 1274-1283 ◽  
Author(s):  
Katarína Valachová ◽  
Andrea Vargová ◽  
Peter Rapta ◽  
Eva Hrabárová ◽  
František Dráfi ◽  
...  
Keyword(s):  
Molar Mass ◽  
High Molar Mass ◽  
Radical Degradation ◽  
Chain Breaking

Novel hydrogels based on a high-molar-mass water-soluble dimethacrylate monomer

2018 ◽  
Vol 67 (5) ◽  
pp. 606-614
Author(s):  
Silvana V Asmussen ◽  
Maria L Gomez ◽  
Claudia I Vallo
Keyword(s):  
Molar Mass ◽  
Water Soluble ◽  
High Molar Mass

Manganese Porphyrins as Pro-Oxidants in High-Molar-Mass Hyaluronan Oxidative Degradation

2020 ◽  
pp. 223-241
Author(s):  
Katarína Valachová ◽  
Peter Rapta ◽  
Ines Batinic-Haberle ◽  
Ladislav Šoltés
Keyword(s):  
Molar Mass ◽  
Oxidative Degradation ◽  
High Molar Mass ◽  
Manganese Porphyrins

scholarly journals Oxidative Degradation of High-Molar-Mass Hyaluronan: Effects of Some Indole Derivatives to Hyaluronan Decay

2020 ◽  
Vol 21 (16) ◽  
pp. 5609
Author(s):  
Katarína Valachová ◽  
Mojmír Mach ◽  
Ladislav Šoltés
Keyword(s):  
Molar Mass ◽  
Radical Scavenging ◽  
Oxidative Degradation ◽  
Protective Effects ◽  
Indole Derivatives ◽  
High Molar Mass ◽  
Positive Effects ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Effective Compound

Indole derivatives such as isatin (a natural compound), cemtirestat, stobadine, and its derivatives (synthetic compounds) are known to have numerous positive effects on human health due to regulation of oxidative status. The aim of the study was to assess radical scavenging capacities of these compounds and explore their potential protective effects against reactive oxygen species formed during Cu(II) ions and ascorbate-induced degradation of high-molar-mass hyaluronan. Based on the IC50 values determined by the ABTS assay, the most effective compound was SM1M3EC2·HCl reaching the value ≈ 11 µmol/L. The lowest IC50 value reached in the DPPH assay was reported for cemtirestat ≈ 3 µmol/L. Great potency of inhibition of hyaluronan degradation was shown by cemtirestat, followed by isatin even at low concentration 10 µmol/L. On the other hand, stobadine·2HCl had also a protective effect on hyaluronan degradation, however at greater concentrations compared to cemtirestat or isatin. SME1i-ProC2·HCl reported to be a less effective compound and SM1M3EC2·HCl can be considered almost ineffective compared to stobadine·2HCl. In conclusion, our results showed that both isatin and cemtirestat were capable of attenuating the degradation of high-molar-mass hyaluronan due to their ability to complex/sequester cupric ions.

Dithiols as more effective than monothiols in protecting biomacromolecules from free-radical-mediated damage: in vitro oxidative degradation of high-molar-mass hyaluronan

2014 ◽  
Vol 68 (10) ◽  
Author(s):  
Mária Baňasová ◽  
Katarína Valachová ◽  
Ivo Juránek ◽  
Ladislav Šoltés
Keyword(s):  
Free Radical ◽  
Molar Mass ◽  
Radical Scavenging ◽  
Oxidative Degradation ◽  
Free Radical Scavenging ◽  
Dpph Assay ◽  
High Molar Mass ◽  
High Concentrations ◽  
Free Radical Scavenging Activities

AbstractOxidative stress and the resulting damage to cellular and extracellular components has been observed in a variety of degenerative processes, including degenerative joint disorders, where high-molar-mass hyaluronan (HA) is often found to be massively degraded. The present study sought to test the hypothesis that dithiols are more effective in protecting biomacromolecules from free-radicalmediated damage than monothiols. The materials/thiols tested included bucillamine (BUC), dithioerythritol (DTE), dithiothreitol (DTT) and glutathione (GSH), as a reference, for their effectiveness in protecting HA from oxidative degradation induced in vitro. Since HA degradation results in a decrease in its dynamic viscosity, rotational viscometry was applied to follow HA oxidative degradation. The free-radical-scavenging activities of the thiols tested were determined by 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assays. It was found that all the dithiols in the concentration range tested protected HA from the oxidative degradation. On the other hand, monothiol GSH exerted protection only at high concentrations (10 μmol L−1 and 100 μmol L−1) and 1 μmol L−1 of GSH even exhibited a prodegradative effect. The ABTS assay revealed free-radical scavenging activities in the following order: BUC, DTT, DTE, GSH, and that of the DPPH assay: BUC, DTE, DTT, GSH. In conclusion, it was demonstrated that dithiols may be more effective than monothiols in affording biomacromolecule protection from oxidative degradation.

Bucillamine as An Efficient H Atom Donor Protects High-Molar-Mass Hyaluronan from Oxidative Degradation by Effective Scavenging of Free Radicals

2021 ◽  
pp. 123-138
Author(s):  
Katarína Valachová ◽  
Jozef Rychlý ◽  
Ivica Janigová ◽  
Katarína Csomorová ◽  
Ivo Juránek ◽  
...  
Keyword(s):  
Free Radicals ◽  
Molar Mass ◽  
Oxidative Degradation ◽  
High Molar Mass

Poly(azomethine) Rotaxanes: Novel Water Soluble Supramolecular Polymers with High Molar Mass

2007 ◽  
Vol 28 (21) ◽  
pp. 2074-2079 ◽  
Author(s):  
Gabriela Horvath ◽  
Dhriti Nepal ◽  
Kurt E. Geckeler
Keyword(s):  
Molar Mass ◽  
Water Soluble ◽  
Supramolecular Polymers ◽  
High Molar Mass

Synthesis of defined high molar mass poly(2-methyl-2-oxazoline)

2019 ◽  
Vol 10 (11) ◽  
pp. 1286-1290 ◽  
Author(s):  
Ondrej Sedlacek ◽  
Bryn D. Monnery ◽  
Richard Hoogenboom
Keyword(s):  
Molar Mass ◽  
Water Soluble ◽  
Soluble Polymer ◽  
Water Soluble Polymer ◽  
High Molar Mass ◽  
First Time

In this communication, we report for the first time the synthesis of defined high molar mass poly(2-methyl-2-oxazoline) (PMeOx), a water-soluble polymer with excellent anti-fouling properties.

scholarly journals Protective effects of manganese(II) chloride on hyaluronan degradation by oxidative system ascorbate plus cupric chloride

2010 ◽  
Vol 3 (1) ◽  
pp. 26-34 ◽  
Author(s):  
Katarína Valachová ◽  
Grigorij Kogan ◽  
Peter Gemeiner ◽  
Ladislav Šoltés
Keyword(s):  
Ascorbic Acid ◽  
Synovial Fluid ◽  
Molar Mass ◽  
Oxidative Degradation ◽  
Joint Inflammation ◽  
Transition Metal Ions ◽  
Cupric Chloride ◽  
Protective Effects ◽  
High Molar Mass ◽  
Oxidative System

Protective effects of manganese(II) chloride on hyaluronan degradation by oxidative system ascorbatepluscupric chlorideThe degradation of several high-molar-mass hyaluronan samples was investigated in the presence of ascorbic acid itself and further by an oxidative system composed of ascorbic acid plus transition metal ions, i.e. Fe(II) or Cu(II) ions. The latter oxidative system imitates conditions in a joint synovial fluid during early phase of acute joint inflammation and can be used as a model for monitoring oxidative degradation of hyaluronan under pathophysiological conditions. The system Cu(II)plusascorbate (the Weissberger oxidative system) resulted in a more significant decrease of hyaluronan molar mass compared to the oxidative system Fe(II) plus ascorbate. Addition of manganese(II) chloride was found to decrease the rate of the oxidative damage of hyaluronan initiated by ascorbate itself and by the Weissberger system.

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