scholarly journals Assessing the Effects of VEGF Releasing Microspheres on the Angiogenic and Foreign Body Response to a 3D Printed Silicone-Based Macroencapsulation Device

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2077
Author(s):  
Ruth E. Levey ◽  
Fergal B. Coulter ◽  
Karina C. Scheiner ◽  
Stefano Deotti ◽  
Scott T. Robinson ◽  
...  
Keyword(s):  
Foreign Body ◽  
Blood Vessels ◽  
Foreign Body Response ◽  
Islet Cell Transplantation ◽  
Clinical Implementation ◽  
Multi Scale ◽  
Significant Difference ◽  
Body Response

Macroencapsulation systems have been developed to improve islet cell transplantation but can induce a foreign body response (FBR). The development of neovascularization adjacent to the device is vital for the survival of encapsulated islets and is a limitation for long-term device success. Previously we developed additive manufactured multi-scale porosity implants, which demonstrated a 2.5-fold increase in tissue vascularity and integration surrounding the implant when compared to a non-textured implant. In parallel to this, we have developed poly(ε-caprolactone-PEG-ε-caprolactone)-b-poly(L-lactide) multiblock copolymer microspheres containing VEGF, which exhibited continued release of bioactive VEGF for 4-weeks in vitro. In the present study, we describe the next step towards clinical implementation of an islet macroencapsulation device by combining a multi-scale porosity device with VEGF releasing microspheres in a rodent model to assess prevascularization over a 4-week period. An in vivo estimation of vascular volume showed a significant increase in vascularity (* p = 0.0132) surrounding the +VEGF vs. −VEGF devices, however, histological assessment of blood vessels per area revealed no significant difference. Further histological analysis revealed significant increases in blood vessel stability and maturity (** p = 0.0040) and vessel diameter size (*** p = 0.0002) surrounding the +VEGF devices. We also demonstrate that the addition of VEGF microspheres did not cause a heightened FBR. In conclusion, we demonstrate that the combination of VEGF microspheres with our multi-scale porous macroencapsulation device, can encourage the formation of significantly larger, stable, and mature blood vessels without exacerbating the FBR.

In vivo modulation of foreign body response on polyurethane by surface entrapment technique

2010 ◽  
Vol 95A (2) ◽  
pp. 413-423 ◽  
Author(s):  
Anand P. Khandwekar ◽  
Deepak P. Patil ◽  
Anand A. Hardikar ◽  
Yogesh S. Shouche ◽  
Mukesh Doble
Keyword(s):  
Foreign Body ◽  
Foreign Body Response ◽  
Body Response

10 Analysis of the in vivo course of foreign body response to a phycogenic bone substitute using FTIR spectroscopy

2021 ◽  
pp. 117-122
Author(s):  
Žarko Mitić ◽  
Sanja Stojanović ◽  
Stevo Najman ◽  
Mike Barbeck ◽  
Miroslav Trajanović
Keyword(s):  
Foreign Body ◽  
Ftir Spectroscopy ◽  
Bone Substitute ◽  
Foreign Body Response ◽  
Body Response

scholarly journals Immune‐Informed Mucin Hydrogels Evade Fibrotic Foreign Body Response In Vivo

2019 ◽  
Vol 29 (46) ◽  
pp. 1902581 ◽  
Author(s):  
Hongji Yan ◽  
Cédric Seignez ◽  
Morgan Hjorth ◽  
Benjamin Winkeljann ◽  
Matthew Blakeley ◽  
...  
Keyword(s):  
Foreign Body ◽  
Foreign Body Response ◽  
Body Response

scholarly journals Application of Epigallocatechin-3-gallate (EGCG) Modified 1-Ethyl-3-(3-dimethylaminopropylcarbodiimide hydrochloride/N-hydroxy-succinimide (EDC/NHS) Cross-Linked Collagen Membrane to Promote Macrophage Adhesion

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4660
Author(s):  
Shengan Rung ◽  
Xiwen Zhao ◽  
Chenyu Chu ◽  
Renli Yang ◽  
Yili Qu ◽  
...  
Keyword(s):  
Foreign Body Response ◽  
Collagen Membrane ◽  
Subcutaneous Implantation ◽  
Natural Polymers ◽  
Implant Dentistry ◽  
Macrophage Adhesion ◽  
In Vitro Angiogenesis ◽  
Body Response

The chemically cross-linking 1-ethyl-3-(3-dimethylaminopropylcarbodiimide hydrochloride/N-hydroxy-succinimide (EDC/NHS) collagen membrane endows such natural polymers with promising mechanical properties. Nevertheless, it is inadequate to advance the modulation of foreign body response (FBR) after implantation or guidance of tissue regeneration. In previous research, macrophages have a strong regulatory effect on regeneration, and such enhanced membranes underwent the modification with Epigallocatechin-3-gallate (EGCG) could adjust the recruitment and phenotypes of macrophages. Accordingly, we develop EGCG-EDC/NHS membranes, prepared with physical immersion, while focusing on the surface morphology through SEM, the biological activity of collagen was determined by FTIR, the activity and adhesion of cell culture in vitro, angiogenesis and monocyte/macrophage recruitment after subcutaneous implantation in vivo, are characterized. It could be concluded that it is hopeful EGCG-EDC/NHS collagen membrane can be used in implant dentistry for it not only retains the advantages of the collagen membrane itself, but also improves cell viability, adhesion, vascularization, and immunoregulation tendency.

scholarly journals Dexamethasone-Enhanced Microdialysis and Penetration Injury

2020 ◽  
Vol 8 ◽  
Author(s):  
Andrea Jaquins-Gerstl ◽  
Adrian C. Michael
Keyword(s):  
Immune Response ◽  
Foreign Body ◽  
Brain Tissue ◽  
Macrophage Polarization ◽  
Foreign Body Response ◽  
Mechanical Trauma ◽  
Microdialysis Probe ◽  
The Impact ◽  
Body Response

Microdialysis probes, electrochemical microsensors, and neural prosthetics are often used for in vivo monitoring, but these are invasive devices that are implanted directly into brain tissue. Although the selectivity, sensitivity, and temporal resolution of these devices have been characterized in detail, less attention has been paid to the impact of the trauma they inflict on the tissue or the effect of any such trauma on the outcome of the measurements they are used to perform. Factors affecting brain tissue reaction to the implanted devices include: the mechanical trauma during insertion, the foreign body response, implantation method, and physical properties of the device (size, shape, and surface characteristics. Modulation of the immune response is an important step toward making these devices with reliable long-term performance. Local release of anti-inflammatory agents such as dexamethasone (DEX) are often used to mitigate the foreign body response. In this article microdialysis is used to locally deliver DEX to the surrounding brain tissue. This work discusses the immune response resulting from microdialysis probe implantation. We briefly review the principles of microdialysis and the applications of DEX with microdialysis in (i) neuronal devices, (ii) dopamine and fast scan cyclic voltammetry, (iii) the attenuation of microglial cells, (iv) macrophage polarization states, and (v) spreading depolarizations. The difficulties and complexities in these applications are herein discussed.

In vivo fluorescence imaging of apoptosis during foreign body response

Biomaterials ◽  
2012 ◽  
Vol 33 (29) ◽  
pp. 6926-6932 ◽  
Author(s):  
Ivonne Bartsch ◽  
Elmar Willbold ◽  
Sergey Yarmolenko ◽  
Frank Witte
Keyword(s):  
Foreign Body ◽  
Fluorescence Imaging ◽  
Foreign Body Response ◽  
In Vivo Fluorescence ◽  
In Vivo Fluorescence Imaging ◽  
Body Response
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